FastQCFastQC Report
Sat 18 Jun 2016
SRR3553686_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553686_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445741
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22540.5056748201309729No Hit
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC19010.42648084874400155No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC17730.39776462115892414No Hit
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT12700.2849188205706901No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11660.26158688565781474No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCC8970.20123793862355044No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8460.18979631669512118No Hit
TCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC8090.1814955321588097No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCG7870.17655993054262453No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC7870.17655993054262453No Hit
AAAACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC7810.175213857374574No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC7540.16915652811834675No Hit
ACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC6440.14447852003742084No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5830.13079344282890737No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTT4530.10162852418781311No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCC207.028635E-445.06
CTATGCG253.886949E-545.01
ACACGTG406.7993824E-945.042
CCGGGAT406.7993824E-945.05
ACCGGCT207.028635E-445.035
CTCGTCA406.7993824E-945.036
GGTCGAT207.028635E-445.026
ACCGCAG253.886949E-545.01
CGGTTAT207.028635E-445.09
GCTATAA207.028635E-445.09
CGACCTA207.028635E-445.010
CACGACC406.7993824E-945.027
TACGGGT253.886949E-545.04
ATCACGC406.7993824E-945.042
TAGCCGT253.886949E-545.044
CGCCTAT253.886949E-545.06
ACGATTG253.886949E-545.024
TCGCTTG207.028635E-445.040
CGAATAT950.045.014
CATACCC207.028635E-445.044