##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553686_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	445741
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.895125196021905	33.0	31.0	34.0	30.0	34.0
2	32.037741648176855	33.0	31.0	34.0	30.0	34.0
3	32.087620389418966	34.0	31.0	34.0	30.0	34.0
4	35.682358140714	37.0	35.0	37.0	33.0	37.0
5	35.58097190969644	37.0	35.0	37.0	33.0	37.0
6	35.548628014923466	37.0	35.0	37.0	33.0	37.0
7	35.673720837885675	37.0	35.0	37.0	35.0	37.0
8	35.570566764107404	37.0	35.0	37.0	35.0	37.0
9	37.341850985213384	39.0	37.0	39.0	34.0	39.0
10	36.855095223459365	39.0	37.0	39.0	32.0	39.0
11	36.811621547041895	39.0	37.0	39.0	33.0	39.0
12	36.72036900352447	39.0	35.0	39.0	33.0	39.0
13	36.60005249685356	39.0	35.0	39.0	32.0	39.0
14	37.70442701030419	40.0	36.0	41.0	33.0	41.0
15	37.82847662656116	40.0	36.0	41.0	33.0	41.0
16	37.93387864253008	40.0	36.0	41.0	33.0	41.0
17	37.86360016242616	40.0	36.0	41.0	33.0	41.0
18	37.811666416147496	39.0	36.0	41.0	33.0	41.0
19	37.73730260397854	39.0	36.0	41.0	33.0	41.0
20	37.66655748517637	39.0	36.0	41.0	33.0	41.0
21	37.53915165982039	39.0	35.0	41.0	33.0	41.0
22	37.51104116516093	39.0	35.0	41.0	33.0	41.0
23	37.43156900531923	39.0	35.0	41.0	33.0	41.0
24	37.404939191144635	39.0	35.0	41.0	32.0	41.0
25	37.32427800000449	39.0	35.0	41.0	32.0	41.0
26	37.29196775706072	39.0	35.0	41.0	32.0	41.0
27	37.23338889624244	39.0	35.0	41.0	32.0	41.0
28	37.12822244307793	39.0	35.0	41.0	32.0	41.0
29	37.07807224374693	39.0	35.0	41.0	32.0	41.0
30	36.95116670891841	39.0	35.0	41.0	31.0	41.0
31	36.838035989509606	39.0	35.0	41.0	31.0	41.0
32	36.73057448159357	39.0	35.0	41.0	31.0	41.0
33	36.618735992426096	39.0	35.0	41.0	31.0	41.0
34	36.53757451075849	39.0	35.0	41.0	30.0	41.0
35	36.37259978328222	39.0	35.0	41.0	30.0	41.0
36	36.24946100986896	39.0	35.0	40.0	30.0	41.0
37	36.119365281632156	38.0	35.0	40.0	30.0	41.0
38	36.04293973406081	38.0	35.0	40.0	30.0	41.0
39	36.01490102997032	38.0	35.0	40.0	30.0	41.0
40	35.853206234113536	38.0	35.0	40.0	29.0	41.0
41	35.660533807749346	38.0	35.0	40.0	28.0	41.0
42	35.59190426727629	38.0	35.0	40.0	28.0	41.0
43	35.530545765365986	38.0	35.0	40.0	28.0	41.0
44	35.44259783147613	38.0	34.0	40.0	27.0	41.0
45	35.38928436020021	38.0	34.0	40.0	27.0	41.0
46	35.2431142748816	38.0	34.0	40.0	27.0	41.0
47	35.09635416082433	37.0	34.0	40.0	26.0	41.0
48	34.93828254524488	37.0	34.0	40.0	26.0	41.0
49	34.93410747496865	37.0	34.0	40.0	26.0	41.0
50	34.84230752836288	37.0	34.0	40.0	26.0	41.0
51	33.47861650599788	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	3.0
13	8.0
14	14.0
15	24.0
16	54.0
17	104.0
18	204.0
19	351.0
20	638.0
21	947.0
22	1365.0
23	2039.0
24	2639.0
25	3403.0
26	4281.0
27	5052.0
28	5980.0
29	7274.0
30	8981.0
31	11403.0
32	14523.0
33	19520.0
34	32532.0
35	41904.0
36	38524.0
37	54971.0
38	85508.0
39	103462.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.56489306570408	21.682770936485536	19.873424253097653	13.878911744712738
2	30.21822089509379	26.553985386132307	24.215183256644554	19.012610462129352
3	29.762350782180686	27.924960907791746	25.20073316118553	17.11195514884204
4	26.47209926840923	27.43095205511721	26.575298211293102	19.52165046518045
5	21.92910232623878	31.517181502262524	25.828451948553084	20.72526422294561
6	21.256514433269544	36.743086231690604	26.348933573532612	15.651465761507243
7	75.89407301549555	8.894627148949727	10.063916040929598	5.14738379462513
8	76.52695175000729	6.643992811969282	9.852133862489652	6.976921575533774
9	70.48375626204455	10.144231739956613	11.955373187568567	7.416638810430272
10	37.79459372146605	24.94453056819992	20.960378336298433	16.300497374035594
11	28.254748833964115	27.36634054305079	23.666658440663973	20.712252182321123
12	24.395332715635313	22.272350984091656	32.17675735460727	21.155558945665756
13	22.294336845836487	27.371949181250994	30.719184459136585	19.61452951377594
14	18.836499222642743	29.549671221628703	29.90054762743387	21.71328192829468
15	17.17297713246033	27.9637726841372	34.528122833663495	20.335127349738976
16	18.950466750871023	24.946325332423985	32.787874572902204	23.31533334380279
17	18.576931446737007	26.586739833221536	27.066166226575522	27.77016249346594
18	20.48140063400046	25.20207923435358	30.652105146262066	23.66441498538389
19	21.364200286713587	28.93990007650182	26.15263123652525	23.543268400259343
20	22.29770202875661	26.81871310918224	27.133694230506055	23.749890631555097
21	21.898591334429636	29.087519433931362	27.507678225696086	21.506211005942912
22	19.15753767322279	26.352747447508758	26.018023919720196	28.47169095954826
23	19.429668798697	29.05745713317824	26.511808426866722	25.00106564125804
24	21.169917059458296	25.95924539138199	29.592745563006318	23.27809198615339
25	19.907524773354933	28.42031583363433	26.42655712622353	25.245602266787216
26	18.09997285419111	27.76253474551365	27.94088944028034	26.196602960014896
27	21.497461530350584	26.923033779706152	26.29823148420271	25.28127320574055
28	18.16121918333741	26.34624142719651	29.435479347872416	26.05706004159366
29	19.55732140413379	25.23393629933078	27.53572141669714	27.67302087983829
30	20.13837632167559	26.104845639059455	26.166091968205752	27.5906860710592
31	20.981691161459235	28.401246463753616	22.77353889366246	27.843523481124688
32	20.794586991100214	27.631516957156734	24.52163924790405	27.052256803839004
33	20.06793182588095	27.285127461911742	23.602719965181574	29.04422074702574
34	18.542830926479727	27.067512299743573	26.04584276519324	28.34381400858346
35	18.71669871068625	27.572065392234503	25.03651223468337	28.674723662395873
36	20.48364408928055	28.53226425211053	24.00429846031664	26.979793198292285
37	18.526229357407104	29.742159684659924	27.282659661103647	24.448951296829325
38	18.711314418014048	29.908624066442176	25.801306139664064	25.578755375879712
39	21.081749266950986	27.498480059047743	26.373387236085527	25.046383437915743
40	21.025662884948883	26.578214703157215	27.89557164362264	24.50055076827126
41	17.834347749029146	29.41685866904772	25.067247572020523	27.681546009902615
42	19.296407555060004	27.67010438797418	26.936494511386655	26.09699354557916
43	21.36038641273744	25.46725564845953	26.89476624317709	26.277591695625937
44	20.175842024852997	25.042345218411587	27.562418534530142	27.219394222205274
45	20.771928092771365	25.197143632737394	26.40143042708658	27.62949784740466
46	22.7638920359581	25.748360595054077	26.48062439847355	25.007122970514267
47	20.17000904112478	26.410628593734923	28.00774440762685	25.411617957513442
48	19.27711383965128	26.041355854633068	27.469315140406646	27.212215165309
49	21.269302128366025	24.534202597472525	29.27843747826653	24.918057795894928
50	20.29766164656157	22.91263312102768	30.189055976452693	26.600649255958057
51	19.18580520975185	24.90863528371857	26.18022573647028	29.725333770059294
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3055.0
1	2926.0
2	2797.0
3	2620.5
4	2444.0
5	1895.0
6	1346.0
7	1844.0
8	2342.0
9	2520.0
10	2698.0
11	2328.5
12	1959.0
13	1870.5
14	1782.0
15	1797.5
16	1813.0
17	1722.0
18	1631.0
19	1686.5
20	1742.0
21	1657.5
22	1573.0
23	1535.5
24	1498.0
25	1871.5
26	2514.5
27	2784.0
28	3415.0
29	4046.0
30	4747.0
31	5448.0
32	6968.5
33	8489.0
34	9439.5
35	10390.0
36	10943.5
37	11497.0
38	11525.5
39	11554.0
40	12971.0
41	14388.0
42	18419.0
43	22450.0
44	26430.5
45	30411.0
46	35475.0
47	40539.0
48	43652.5
49	46766.0
50	45059.5
51	43353.0
52	37840.0
53	32327.0
54	28957.5
55	25588.0
56	23470.0
57	21352.0
58	19851.0
59	18350.0
60	16929.5
61	15509.0
62	14067.0
63	12625.0
64	10779.0
65	8933.0
66	8045.0
67	7157.0
68	6613.0
69	6069.0
70	5361.0
71	4653.0
72	4337.5
73	4022.0
74	3586.5
75	2474.0
76	1797.0
77	1613.0
78	1429.0
79	1157.0
80	885.0
81	662.0
82	439.0
83	295.5
84	152.0
85	138.5
86	125.0
87	91.0
88	57.0
89	46.0
90	35.0
91	22.5
92	10.0
93	19.0
94	28.0
95	18.0
96	8.0
97	4.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	445741.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.011225121933343
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.47857041377625	23.406829389833128
2	9.9230170909509	6.15449833792541
3	3.7082343263046753	3.449906684937454
4	1.8385549461530548	2.2806336533678566
5	1.1432029441096763	1.772606192992108
6	0.794715968058362	1.478706947607183
7	0.5893796417810627	1.2794169327489877
8	0.44928860924448233	1.1146392164800787
9	0.35793275799860746	0.9989940003128374
>10	4.958543616516771	37.44230357776581
>50	0.6795883329794898	13.31626017665137
>100	0.06882824267915942	3.878404588806033
>500	0.006520570359078261	1.5475872325162758
>1k	0.0036225390883768117	1.8792130680554777
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2254	0.5056748201309729	No Hit
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	1901	0.42648084874400155	No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	1773	0.39776462115892414	No Hit
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT	1270	0.2849188205706901	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1166	0.26158688565781474	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCC	897	0.20123793862355044	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	846	0.18979631669512118	No Hit
TCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	809	0.1814955321588097	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCG	787	0.17655993054262453	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	787	0.17655993054262453	No Hit
AAAACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC	781	0.175213857374574	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC	754	0.16915652811834675	No Hit
ACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	644	0.14447852003742084	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	583	0.13079344282890737	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTT	453	0.10162852418781311	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2434552800841743E-4	0.0	0.0	0.2947900238030605	0.0
2	2.2434552800841743E-4	0.0	0.0	1.4995255092082622	0.0
3	2.2434552800841743E-4	0.0	0.0	1.9410375083288278	0.0
4	2.2434552800841743E-4	0.0	0.0	2.597921214337474	0.0
5	2.2434552800841743E-4	0.0	0.0	4.692186718296051	0.0
6	2.2434552800841743E-4	0.0	0.0	6.116107784565476	0.0
7	2.2434552800841743E-4	0.0	0.0	7.422247448630483	0.0
8	4.4869105601683487E-4	0.0	0.0	9.292840461164667	0.0
9	4.4869105601683487E-4	0.0	0.0	9.99571500041504	0.0
10	4.4869105601683487E-4	0.0	0.0	11.576004899706332	0.0
11	4.4869105601683487E-4	0.0	0.0	13.270711018281917	0.0
12	4.4869105601683487E-4	0.0	0.0	14.738155116984975	0.0
13	4.4869105601683487E-4	0.0	0.0	15.27344354681306	0.0
14	4.4869105601683487E-4	0.0	0.0	15.480738814692838	0.0
15	4.4869105601683487E-4	0.0	0.0	15.861004484667104	0.0
16	4.4869105601683487E-4	0.0	0.0	16.639034775800297	0.0
17	4.4869105601683487E-4	0.0	0.0	17.5586270951068	0.0
18	4.4869105601683487E-4	0.0	0.0	18.710192690374008	0.0
19	8.973821120336697E-4	0.0	0.0	19.22820651454544	0.0
20	8.973821120336697E-4	0.0	0.0	19.72445882250006	0.0
21	8.973821120336697E-4	0.0	0.0	20.27500274823272	0.0
22	8.973821120336697E-4	0.0	0.0	20.89419640553595	0.0
23	8.973821120336697E-4	0.0	0.0	21.545471473344385	0.0
24	0.0017947642240673395	0.0	0.0	22.045088964219133	0.0
25	0.0017947642240673395	0.0	0.0	22.478748869859402	0.0
26	0.0017947642240673395	0.0	0.0	22.863501450393837	0.0
27	0.0031408373921178443	0.0	0.0	23.261490417080772	0.0
28	0.0031408373921178443	0.0	0.0	23.678548753648418	0.0
29	0.0031408373921178443	0.0	0.0	24.087979342263782	0.0
30	0.0031408373921178443	0.0	0.0	24.552374585241203	0.0
31	0.0031408373921178443	0.0	0.0	24.96988161286487	0.0
32	0.0031408373921178443	0.0	0.0	25.39658680713688	0.0
33	0.0031408373921178443	0.0	0.0	25.79502446487983	0.0
34	0.0031408373921178443	0.0	0.0	26.165867622677744	0.0
35	0.0031408373921178443	0.0	0.0	26.6098474226064	0.0
36	0.004711256088176767	0.0	0.0	26.98697225518855	0.0
37	0.004711256088176767	0.0	0.0	27.358264104042483	0.0
38	0.004711256088176767	0.0	0.0	27.72035778624807	0.0
39	0.004711256088176767	0.0	0.0	28.064728171740988	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCCC	20	7.028635E-4	45.0	6
CTATGCG	25	3.886949E-5	45.0	1
ACACGTG	40	6.7993824E-9	45.0	42
CCGGGAT	40	6.7993824E-9	45.0	5
ACCGGCT	20	7.028635E-4	45.0	35
CTCGTCA	40	6.7993824E-9	45.0	36
GGTCGAT	20	7.028635E-4	45.0	26
ACCGCAG	25	3.886949E-5	45.0	1
CGGTTAT	20	7.028635E-4	45.0	9
GCTATAA	20	7.028635E-4	45.0	9
CGACCTA	20	7.028635E-4	45.0	10
CACGACC	40	6.7993824E-9	45.0	27
TACGGGT	25	3.886949E-5	45.0	4
ATCACGC	40	6.7993824E-9	45.0	42
TAGCCGT	25	3.886949E-5	45.0	44
CGCCTAT	25	3.886949E-5	45.0	6
ACGATTG	25	3.886949E-5	45.0	24
TCGCTTG	20	7.028635E-4	45.0	40
CGAATAT	95	0.0	45.0	14
CATACCC	20	7.028635E-4	45.0	44
>>END_MODULE