##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553685_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	655452
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08686067019401	33.0	31.0	34.0	30.0	34.0
2	32.217236349877645	34.0	31.0	34.0	30.0	34.0
3	32.25832860377266	34.0	31.0	34.0	30.0	34.0
4	35.80479882584842	37.0	35.0	37.0	35.0	37.0
5	35.733580796152886	37.0	35.0	37.0	35.0	37.0
6	35.7080396428724	37.0	35.0	37.0	33.0	37.0
7	35.87045580759537	37.0	35.0	37.0	35.0	37.0
8	35.7723692963024	37.0	35.0	37.0	35.0	37.0
9	37.5887555457913	39.0	37.0	39.0	35.0	39.0
10	37.05480645417208	39.0	37.0	39.0	33.0	39.0
11	36.99206959472242	39.0	37.0	39.0	33.0	39.0
12	36.87614653704619	39.0	35.0	39.0	33.0	39.0
13	36.79593166242532	39.0	35.0	39.0	33.0	39.0
14	37.90251460061149	40.0	37.0	41.0	33.0	41.0
15	38.02962688343311	40.0	37.0	41.0	33.0	41.0
16	38.12686359947029	40.0	37.0	41.0	33.0	41.0
17	38.06337458730769	40.0	37.0	41.0	33.0	41.0
18	37.997145481286196	40.0	37.0	41.0	33.0	41.0
19	37.91627457083051	40.0	36.0	41.0	33.0	41.0
20	37.82491166401201	40.0	36.0	41.0	33.0	41.0
21	37.72859034681411	40.0	36.0	41.0	33.0	41.0
22	37.70262658440282	40.0	36.0	41.0	33.0	41.0
23	37.621789238571246	39.0	35.0	41.0	33.0	41.0
24	37.58428534812618	39.0	35.0	41.0	33.0	41.0
25	37.499644520117414	39.0	35.0	41.0	33.0	41.0
26	37.41272587466359	39.0	35.0	41.0	33.0	41.0
27	37.355530229521	39.0	35.0	41.0	33.0	41.0
28	37.27385071675729	39.0	35.0	41.0	32.0	41.0
29	37.17996893746606	39.0	35.0	41.0	32.0	41.0
30	37.04508339283426	39.0	35.0	41.0	32.0	41.0
31	36.9357985024075	39.0	35.0	41.0	31.0	41.0
32	36.80931784478497	39.0	35.0	41.0	31.0	41.0
33	36.673977652063	39.0	35.0	41.0	31.0	41.0
34	36.524657793400586	39.0	35.0	41.0	30.0	41.0
35	36.35580790050225	39.0	35.0	41.0	30.0	41.0
36	36.26754972141362	39.0	35.0	41.0	30.0	41.0
37	36.11322263110037	38.0	35.0	40.0	30.0	41.0
38	36.03241274723397	38.0	35.0	40.0	30.0	41.0
39	35.99552980233488	38.0	35.0	40.0	29.0	41.0
40	35.82489640736469	38.0	35.0	40.0	29.0	41.0
41	35.7213663243075	38.0	35.0	40.0	29.0	41.0
42	35.570176000683496	38.0	35.0	40.0	28.0	41.0
43	35.43990864319584	38.0	34.0	40.0	27.0	41.0
44	35.30635500387519	38.0	34.0	40.0	27.0	41.0
45	35.232303814772095	38.0	34.0	40.0	27.0	41.0
46	35.15366495181951	37.0	34.0	40.0	26.0	41.0
47	35.03770832951917	37.0	34.0	40.0	26.0	41.0
48	34.86605579050792	37.0	34.0	40.0	26.0	41.0
49	34.790626926151724	37.0	34.0	40.0	26.0	41.0
50	34.70530260034297	37.0	34.0	40.0	26.0	41.0
51	33.3363663548208	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	3.0
11	3.0
12	11.0
13	11.0
14	26.0
15	35.0
16	77.0
17	166.0
18	296.0
19	510.0
20	930.0
21	1360.0
22	2034.0
23	2780.0
24	3833.0
25	5073.0
26	6392.0
27	7477.0
28	8900.0
29	10208.0
30	12628.0
31	15738.0
32	20296.0
33	27722.0
34	46179.0
35	58160.0
36	56632.0
37	79967.0
38	126136.0
39	161801.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.893752708054905	19.761935275199406	20.889706645185306	14.454605371560389
2	29.071236337672325	26.95422395537736	26.503237460561568	17.47130224638875
3	31.89615715567273	26.4901167438653	25.860169775971393	15.753556324490582
4	28.346545589913525	27.199550844302863	26.363181438152605	18.09072212763101
5	23.19391809011186	32.735120191867594	24.445420869873004	19.625540848147537
6	23.787401670908014	36.90811836717258	24.170343518671086	15.134136443248321
7	81.35515644166163	7.136144218035797	7.65837925584177	3.8503200844607997
8	82.68523095512715	5.645112075331222	7.420222991157248	4.249433978384382
9	77.55289479626273	7.967478930570049	8.888065029933543	5.591561243233676
10	39.409445695489524	26.56624741399828	17.231162617552467	16.79314427295973
11	30.61658214484051	27.67006584768984	21.804952917986366	19.90839908948329
12	27.848110921928686	22.780920647125953	29.203358903474243	20.16760952747112
13	24.1828539694745	25.74116792686574	28.842234061380545	21.23374404227922
14	20.149911816578484	29.934762576054386	28.27575474634298	21.639570861024147
15	20.692285628848488	27.278732843900087	31.236154592555977	20.792826934695448
16	22.18362290449949	24.192007957867244	30.96962096385395	22.654748173779314
17	22.18484343628519	24.084601160725725	28.2722457174591	25.458309685529983
18	22.96811970975754	26.04477520855837	28.527489427143404	22.45961565454068
19	24.90373055540299	27.479357756174366	25.803109914989964	21.813801773432683
20	26.39430499868793	25.875273856819415	25.695703117848446	22.03471802664421
21	24.452744060587197	27.517957073897097	26.60149026931034	21.427808596205367
22	22.2798923490965	26.023873601728276	25.344800229459974	26.351433819715247
23	22.525066671548792	28.379194815180973	25.589059153072995	23.50667936019724
24	22.300336256506963	26.6539731360954	27.91005291005291	23.135637697344734
25	23.39210193881474	27.353795548720576	26.220379219225816	23.033723293238864
26	20.3497738964867	28.304894942726545	26.689673690827092	24.65565746995966
27	21.864911541958833	28.314659197012137	26.598591506319302	23.221837754709725
28	20.568401652600038	28.579819727455252	28.38148331227916	22.47029530766555
29	22.51209852132574	29.087255817359626	25.650543441777586	22.75010221953705
30	22.917620207124244	27.813173199563053	27.238912994391658	22.030293598921048
31	24.410025448087733	29.167505782269338	24.2469318882237	22.175536881419234
32	24.224657183134692	29.381251411239877	24.797544290047174	21.596547115578257
33	24.278208015232234	28.175671139915657	23.57090984541965	23.975210999432452
34	23.19895278372787	27.445793132067642	26.29254316105527	23.062710923149215
35	24.07819336885081	27.9567382508559	24.7349920360301	23.230076344263196
36	25.227781744506082	28.666630050713092	24.478375228086875	21.627212976693947
37	23.561755857026906	29.817286391680852	25.963762411282598	20.657195340009643
38	24.20406070925102	29.17681233713529	25.186436230265524	21.432690723348163
39	26.23426276828814	25.975204896773523	24.794035261163287	22.996497073775043
40	25.784649371731263	25.292469929148133	26.98443211707341	21.938448582047197
41	23.115193789934274	26.02753519708537	26.492862940383127	24.36440807259723
42	23.480437926804708	27.659386194564973	25.9564392205684	22.90373665806192
43	23.24624839042371	26.740020626987178	26.623612407926135	23.39011857466298
44	23.820813728541527	26.192612121101167	26.388812605652284	23.59776154470503
45	24.701274845450165	26.559687055650144	25.683497799991457	23.055540298908234
46	24.849722023885807	26.85719167841428	25.844455429230518	22.44863086846939
47	24.380885251704168	27.384308843363055	26.92630429077949	21.308501614153286
48	23.882603150192537	26.38988057096477	26.983669284707346	22.743846994135346
49	23.886264745549635	26.33571947297437	27.28147904041791	22.49653674105808
50	22.608825665342387	25.322983223790608	27.998388898042876	24.069802212824126
51	23.430090990644622	25.456021188431798	25.610876158742364	25.503011662181212
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3587.0
1	3336.0
2	3085.0
3	3361.0
4	3637.0
5	2771.5
6	1906.0
7	2187.5
8	2469.0
9	2734.5
10	3000.0
11	2840.5
12	2681.0
13	2635.0
14	2589.0
15	2523.5
16	2458.0
17	2386.0
18	2314.0
19	2316.5
20	2319.0
21	2675.0
22	3031.0
23	2768.5
24	2506.0
25	3120.5
26	4220.5
27	4706.0
28	6003.0
29	7300.0
30	9114.5
31	10929.0
32	11212.0
33	11495.0
34	13653.5
35	15812.0
36	16557.0
37	17302.0
38	18731.0
39	20160.0
40	22896.0
41	25632.0
42	29134.0
43	32636.0
44	36617.5
45	40599.0
46	44686.5
47	48774.0
48	54146.0
49	59518.0
50	56239.5
51	52961.0
52	47591.0
53	42221.0
54	38839.0
55	35457.0
56	33890.0
57	32323.0
58	31763.0
59	31203.0
60	29696.5
61	28190.0
62	25685.0
63	23180.0
64	21062.0
65	18944.0
66	16071.5
67	13199.0
68	12262.5
69	11326.0
70	10591.5
71	9857.0
72	9040.0
73	8223.0
74	6825.0
75	4479.5
76	3532.0
77	2899.5
78	2267.0
79	1724.5
80	1182.0
81	1040.5
82	899.0
83	638.0
84	377.0
85	293.0
86	209.0
87	164.5
88	120.0
89	107.0
90	94.0
91	79.0
92	64.0
93	38.0
94	12.0
95	6.5
96	1.0
97	2.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	655452.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.174549837667445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.02292963100814	20.387143402256484
2	9.666921138822534	5.253884605274677
3	3.3880207991417692	2.7620382017199567
4	1.733390370582165	1.884164120540715
5	1.0616342448153042	1.442471634755396
6	0.7408726833241456	1.2079728993815042
7	0.6007341454089821	1.1427275981523461
8	0.4835933757502564	1.0513145832392945
9	0.4214823962172546	1.0308234943534753
>10	5.624951585180408	37.44800558352275
>50	1.1510353305118282	20.31377558069482
>100	0.09822469225554578	4.232388127799741
>500	0.00225803890242634	0.37384305427502607
>1k	0.003951568079246094	1.4694471140338428
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2619	0.3995715933432197	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1388	0.21176226481878155	No Hit
GCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	1182	0.18033357133703154	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1134	0.17301038062283738	No Hit
CCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	1128	0.1720949817835631	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCC	1073	0.16370382575688228	No Hit
CTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGCT	1055	0.16095762923905946	Illumina Single End Adapter 2 (95% over 21bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	802	0.12235831151632766	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1678231205336165	0.0
2	0.0	0.0	0.0	0.6639692914202718	0.0
3	0.0	0.0	0.0	0.8783251862836637	0.0
4	0.0	0.0	0.0	1.1791862714584744	0.0
5	0.0	0.0	0.0	2.1461525786785303	0.0
6	1.5256647321237863E-4	0.0	0.0	2.8102744365720143	0.0
7	1.5256647321237863E-4	0.0	0.0	3.3985707572789465	0.0
8	1.5256647321237863E-4	0.0	0.0	4.190238186777979	0.0
9	1.5256647321237863E-4	0.0	0.0	4.545107803469972	0.0
10	1.5256647321237863E-4	0.0	0.0	5.473627359440508	0.0
11	1.5256647321237863E-4	0.0	0.0	6.614672012595888	0.0
12	1.5256647321237863E-4	0.0	0.0	7.5000152566473215	0.0
13	1.5256647321237863E-4	0.0	0.0	7.844510353160872	0.0
14	1.5256647321237863E-4	0.0	0.0	7.988380537400145	0.0
15	1.5256647321237863E-4	0.0	0.0	8.25552443199502	0.0
16	1.5256647321237863E-4	0.0	0.0	8.79942390899715	0.0
17	1.5256647321237863E-4	0.0	0.0	9.49848348925627	0.0
18	1.5256647321237863E-4	0.0	0.0	10.343549184379635	0.0
19	1.5256647321237863E-4	0.0	0.0	10.735645020535447	0.0
20	3.0513294642475725E-4	0.0	0.0	11.122248463655614	0.0
21	3.0513294642475725E-4	0.0	0.0	11.570488761953584	0.0
22	3.0513294642475725E-4	0.0	0.0	12.054734747929674	0.0
23	3.0513294642475725E-4	0.0	0.0	12.575138988057097	0.0
24	3.0513294642475725E-4	0.0	0.0	12.983712003319846	0.0
25	3.0513294642475725E-4	0.0	0.0	13.311272221306824	0.0
26	3.0513294642475725E-4	0.0	0.0	13.61060764174951	0.0
27	3.0513294642475725E-4	0.0	0.0	13.93328573259369	0.0
28	3.0513294642475725E-4	0.0	0.0	14.269084540134136	0.0
29	3.0513294642475725E-4	0.0	0.0	14.60091661937106	0.0
30	3.0513294642475725E-4	0.0	0.0	14.960515796732636	0.0
31	3.0513294642475725E-4	0.0	0.0	15.301654430835516	0.0
32	4.576994196371359E-4	0.0	0.0	15.639741735474146	0.0
33	4.576994196371359E-4	0.0	0.0	15.97294691296998	0.0
34	4.576994196371359E-4	0.0	0.0	16.310576518188974	0.0
35	6.102658928495145E-4	0.0	0.0	16.68360154519324	0.0
36	6.102658928495145E-4	0.0	0.0	17.00490653777851	0.0
37	6.102658928495145E-4	0.0	0.0	17.36008128741693	0.0
38	6.102658928495145E-4	0.0	0.0	17.677419551698677	0.0
39	6.102658928495145E-4	0.0	0.0	17.996283480712545	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	45	3.8380676E-10	45.0	1
CGTTCTA	25	3.8889655E-5	45.0	27
TCGATAC	20	7.031065E-4	45.0	25
GAGCGTA	20	7.031065E-4	45.0	8
CGGATAG	25	3.8889655E-5	45.0	29
TACGAAG	20	7.031065E-4	45.0	1
TCATCGA	25	3.8889655E-5	45.0	16
CGTCGTT	20	7.031065E-4	45.0	20
AGTTAGT	25	3.8889655E-5	45.0	30
GATTCGC	20	7.031065E-4	45.0	11
TCGAACG	20	7.031065E-4	45.0	26
TAGTACG	25	3.8889655E-5	45.0	1
TCGATAG	30	2.164068E-6	44.999996	1
CGTTAGG	60	0.0	44.999996	2
TATACGG	30	2.164068E-6	44.999996	2
ATAACGC	100	0.0	42.75	11
TAGGGCA	280	0.0	42.589283	5
GTAGGGT	220	0.0	41.93182	4
CGCATCG	70	0.0	41.785713	21
TCGTTGC	60	3.6379788E-12	41.249996	16
>>END_MODULE