FastQCFastQC Report
Sat 18 Jun 2016
SRR3553683_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553683_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427091
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52500.36788123532416644No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27720.19424129225115988No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT15470.10840233734218771No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAC253.8912804E-545.00000414
GTCGGTA253.8912804E-545.0000041
ACGCGTC207.0338603E-445.015
CGAATAT2250.041.014
CATGACG501.0822987E-940.5000041
TTAACGG1000.040.5000042
CGTAAGG2050.039.5121962
GCGTACG403.4590084E-739.3751
CATCGTT1550.039.1935536
TACGAAT2300.039.13043212
GCGTAAG1500.039.01
ACACGAC6650.038.57142626
ATAACGC1650.038.18181611
CAATGCG659.094947E-1238.076921
CGGCATC1600.037.9687533
CGTATGG1550.037.741942
CGAGCAT601.5643309E-1037.5000046
TATCGAG601.5643309E-1037.5000041
GCGAGAC6900.037.521
GCTACGA2550.037.05882310
Produced by FastQC (version 0.11.5)