##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553683_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1427091
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23658056844308	33.0	31.0	34.0	30.0	34.0
2	32.392753510462896	34.0	31.0	34.0	30.0	34.0
3	32.4094854497716	34.0	31.0	34.0	30.0	34.0
4	35.9306953796219	37.0	35.0	37.0	35.0	37.0
5	35.85455797843305	37.0	35.0	37.0	35.0	37.0
6	35.813639774898725	37.0	35.0	37.0	35.0	37.0
7	35.942022618039076	37.0	35.0	37.0	35.0	37.0
8	35.86095630902304	37.0	35.0	37.0	35.0	37.0
9	37.641743939244236	39.0	38.0	39.0	35.0	39.0
10	37.15420670440778	39.0	37.0	39.0	34.0	39.0
11	37.05896190221927	39.0	37.0	39.0	33.0	39.0
12	36.92597668964348	39.0	37.0	39.0	33.0	39.0
13	36.89228157139243	39.0	37.0	39.0	33.0	39.0
14	38.06247534319816	40.0	37.0	41.0	33.0	41.0
15	38.1557356888944	40.0	37.0	41.0	33.0	41.0
16	38.22367459398174	40.0	37.0	41.0	33.0	41.0
17	38.14629760821139	40.0	37.0	41.0	33.0	41.0
18	38.08379143306208	40.0	37.0	41.0	33.0	41.0
19	37.99840724943259	40.0	37.0	41.0	33.0	41.0
20	37.92728354393658	40.0	36.0	41.0	33.0	41.0
21	37.82435177574521	40.0	36.0	41.0	33.0	41.0
22	37.777157868699334	40.0	36.0	41.0	33.0	41.0
23	37.67110436545392	40.0	36.0	41.0	33.0	41.0
24	37.630910712771644	40.0	35.0	41.0	33.0	41.0
25	37.53853117986169	40.0	35.0	41.0	33.0	41.0
26	37.44790346235804	40.0	35.0	41.0	33.0	41.0
27	37.377892510008124	39.0	35.0	41.0	33.0	41.0
28	37.26050546181008	39.0	35.0	41.0	32.0	41.0
29	37.16536226491513	39.0	35.0	41.0	32.0	41.0
30	36.99143572484165	39.0	35.0	41.0	31.0	41.0
31	36.85883170729827	39.0	35.0	41.0	31.0	41.0
32	36.70339382702294	39.0	35.0	41.0	31.0	41.0
33	36.57915998349089	39.0	35.0	41.0	30.0	41.0
34	36.394830462808606	39.0	35.0	41.0	30.0	41.0
35	36.22241538906769	39.0	35.0	41.0	30.0	41.0
36	36.08887379991886	39.0	35.0	41.0	29.0	41.0
37	36.01556102589113	39.0	35.0	41.0	29.0	41.0
38	35.885836292149556	38.0	35.0	41.0	29.0	41.0
39	35.808169205747916	38.0	35.0	41.0	28.0	41.0
40	35.676560219355316	38.0	35.0	40.0	28.0	41.0
41	35.514346317088396	38.0	34.0	40.0	27.0	41.0
42	35.39025752387199	38.0	34.0	40.0	27.0	41.0
43	35.28930951144671	38.0	34.0	40.0	26.0	41.0
44	35.159270852384324	38.0	34.0	40.0	26.0	41.0
45	35.06709733296615	38.0	34.0	40.0	26.0	41.0
46	34.91858823298584	37.0	34.0	40.0	25.0	41.0
47	34.80123341819127	37.0	34.0	40.0	25.0	41.0
48	34.62892555555322	37.0	34.0	40.0	24.0	41.0
49	34.573648772222654	37.0	33.0	40.0	24.0	41.0
50	34.478542013088166	36.0	33.0	40.0	24.0	41.0
51	33.05900044215821	35.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	5.0
11	9.0
12	21.0
13	35.0
14	55.0
15	85.0
16	201.0
17	391.0
18	678.0
19	1288.0
20	2118.0
21	3216.0
22	4729.0
23	6684.0
24	9220.0
25	12343.0
26	15387.0
27	18190.0
28	20294.0
29	23836.0
30	28107.0
31	34596.0
32	43734.0
33	59196.0
34	97668.0
35	115318.0
36	120423.0
37	168773.0
38	265145.0
39	375222.0
40	118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.26087754740237	18.670778527788347	22.983607912880117	14.084736011929161
2	32.97189877870437	23.90415187258556	24.582524870523322	18.541424478186745
3	32.6195736641882	25.12103292642165	25.064133962024847	17.195259447365306
4	27.728645195015595	27.163159181860163	25.567745855029568	19.540449768094675
5	24.528288665544103	31.486219168924755	24.454572273246768	19.530919892284373
6	24.720988360237715	36.25781397261982	24.680276170195175	14.340921496947287
7	82.47392773130795	6.284392515964293	7.404363141523561	3.837316611204191
8	84.35215413733252	4.921620275091077	7.2186006358389205	3.5076249517374856
9	78.8442362820591	7.55284701536202	8.850591868353174	4.752324834225708
10	40.75591535508247	25.953495607498052	16.555496460982518	16.735092576436962
11	31.646615387526094	27.51590473207385	21.921727486193944	18.915752394206116
12	27.391595910842405	24.36326765427012	28.179702625831148	20.065433809056323
13	25.393965766724058	24.746634937786027	28.846513642087295	21.01288565340262
14	22.431996277742623	27.027919032493376	29.051476044625048	21.488608645138957
15	22.92923156266839	25.380091388706116	30.21138806144808	21.479288987177412
16	25.924976052683395	24.030843162769578	29.07908465542842	20.965096129118603
17	25.34113101406988	24.22424358362571	27.279970233152618	23.15465516915179
18	26.05222792379743	23.561286561263437	27.552132274676243	22.834353240262885
19	25.964216717784637	25.80332999086954	25.818535748596272	22.41391754274955
20	27.055457570680495	25.566834911018287	25.785601618957728	21.59210589934349
21	26.837111298438572	26.11045826790303	25.774810436054885	21.277619997603516
22	25.71503849439174	23.61278993420882	26.091538661514928	24.580632909884514
23	24.417013350935573	26.866471724648257	25.956508729996898	22.760006194419276
24	23.834569764647103	26.12972823737239	28.028275702110093	22.00742629587041
25	24.919854445161523	26.195876787114486	26.137716515625144	22.74655225209885
26	23.503967161169122	26.27533913394451	25.947329217267857	24.27336448761852
27	23.70458506149923	25.576504932061095	27.358241345506347	23.360668660933325
28	22.466051569241205	27.37603979003441	27.830881142127588	22.327027498596795
29	24.051935020261496	26.681760308207398	26.513866319667073	22.752438351864036
30	24.17848616521301	25.303151656061175	28.011528346825816	22.506833831899996
31	25.6587701835412	26.844188632680044	24.913968345396334	22.583072838382414
32	25.145207979028665	26.766968609570096	25.583021685372554	22.504801726028685
33	24.94823385474367	26.367834987397444	24.31337595149854	24.37055520636035
34	23.374543038951266	27.53615571817074	26.47126216898572	22.618039073892273
35	24.19957802270493	27.437283256638857	25.463057366348746	22.90008135430747
36	24.032384760327126	29.474224138474703	24.50733695328469	21.986054147913485
37	24.10841354896079	27.9479024112688	24.525485760894014	23.4181982788764
38	24.34175536108069	27.778677043019684	24.754062635108763	23.12550496079087
39	24.536837524726874	26.20043150717088	24.71293000936871	24.549800958733535
40	24.81930024083958	25.766962303034635	26.397265486223375	23.016471969902412
41	22.88179240146564	26.43132077772195	26.357884675889625	24.329002144922786
42	23.59232873026317	26.92596337584639	25.78665270820151	23.69505518568893
43	23.264879394516537	25.983276469405244	26.115012987959425	24.636831148118794
44	23.234537951679325	25.96772034859725	26.535799048554015	24.261942651169406
45	23.849425159292576	25.95216422778926	25.982715888475227	24.21569472444294
46	24.30062273534063	26.049705309612353	25.916497266116878	23.733174688930138
47	22.75412009465409	27.63229534766879	26.649036396417607	22.96454816125951
48	22.98374805811262	27.629072007321188	26.451221400737584	22.935958533828607
49	24.25472517169543	26.71154117011459	25.905005357051515	23.12872830113847
50	22.739965426171143	26.56845288772755	26.95721576269488	23.734365923406425
51	23.094883227488648	26.437417095335896	25.61644632332486	24.851253353850595
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3617.0
1	3730.5
2	3844.0
3	7022.5
4	10201.0
5	7404.0
6	4607.0
7	4994.0
8	5381.0
9	6206.0
10	7031.0
11	6338.0
12	5645.0
13	5583.5
14	5522.0
15	5434.0
16	5346.0
17	5011.5
18	4677.0
19	4759.0
20	4841.0
21	5235.5
22	5630.0
23	5881.5
24	6133.0
25	7238.5
26	10633.0
27	12922.0
28	15502.5
29	18083.0
30	20291.0
31	22499.0
32	25296.0
33	28093.0
34	31865.0
35	35637.0
36	38298.5
37	40960.0
38	43525.5
39	46091.0
40	49934.5
41	53778.0
42	59749.0
43	65720.0
44	69653.5
45	73587.0
46	79504.0
47	85421.0
48	91074.0
49	96727.0
50	97409.5
51	98092.0
52	95443.0
53	92794.0
54	89550.5
55	86307.0
56	82401.5
57	78496.0
58	76754.0
59	75012.0
60	70501.5
61	65991.0
62	62461.5
63	58932.0
64	54602.0
65	50272.0
66	44425.5
67	38579.0
68	35613.5
69	32648.0
70	28826.0
71	25004.0
72	23022.5
73	21041.0
74	18726.0
75	13755.0
76	11099.0
77	9098.0
78	7097.0
79	5837.5
80	4578.0
81	3344.0
82	2110.0
83	1640.0
84	1170.0
85	890.0
86	610.0
87	421.5
88	233.0
89	169.0
90	105.0
91	84.0
92	63.0
93	69.0
94	75.0
95	48.5
96	22.0
97	14.5
98	7.0
99	6.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1427091.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.693707199774312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.18664901460336	17.289575052693827
2	7.881746795992845	3.5773210802204183
3	2.860078775996896	1.947173709322874
4	1.5660206446315776	1.4215525591228337
5	0.9897454275700615	1.1230496467795206
6	0.7518605675024411	1.023750214437393
7	0.5857376966510217	0.930479184856796
8	0.481876827023099	0.8748457295054801
9	0.4343195346541148	0.8870688315524443
>10	6.17507314411126	35.051004693002284
>50	1.916222523750875	29.064201836889037
>100	0.16569508620082157	5.441054105286513
>500	0.0027978532378393835	0.3995867805739287
>1k	0.0018652354918929225	0.5989568797797767
>5k	3.108725819821537E-4	0.37037969597689374
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5250	0.36788123532416644	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2772	0.19424129225115988	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1547	0.10840233734218771	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03699834138117331	0.0
2	0.0	0.0	0.0	0.1531787391273577	0.0
3	0.0	0.0	0.0	0.22290099229831875	0.0
4	0.0	0.0	0.0	0.3164479349950354	0.0
5	0.0	0.0	0.0	0.6060580579654696	0.0
6	7.007261625222217E-5	0.0	0.0	0.906739654303755	0.0
7	7.007261625222217E-5	0.0	0.0	1.1396610307261414	0.0
8	7.007261625222217E-5	0.0	0.0	1.5203655548244646	0.0
9	7.007261625222217E-5	0.0	0.0	1.7134156125993367	0.0
10	7.007261625222217E-5	0.0	0.0	2.105191610065511	0.0
11	7.007261625222217E-5	0.0	0.0	2.584908740928224	0.0
12	7.007261625222217E-5	0.0	0.0	2.9591665843313426	0.0
13	7.007261625222217E-5	0.0	0.0	3.112555541307457	0.0
14	7.007261625222217E-5	0.0	0.0	3.1869025871510646	0.0
15	7.007261625222217E-5	0.0	0.0	3.2931326733894335	0.0
16	7.007261625222217E-5	0.0	0.0	3.513230761037663	0.0
17	7.007261625222217E-5	0.0	0.0	3.7835709145387364	0.0
18	7.007261625222217E-5	0.0	0.0	4.083341566865743	0.0
19	7.007261625222217E-5	0.0	0.0	4.256280783776227	0.0
20	7.007261625222217E-5	0.0	0.0	4.423403973537777	0.0
21	7.007261625222217E-5	0.0	0.0	4.6395079220596305	0.0
22	7.007261625222217E-5	0.0	0.0	4.854841071802709	0.0
23	7.007261625222217E-5	0.0	0.0	5.068212188290726	0.0
24	7.007261625222217E-5	0.0	0.0	5.257057889090464	0.0
25	7.007261625222217E-5	0.0	0.0	5.432519720186029	0.0
26	7.007261625222217E-5	0.0	0.0	5.588921799660989	0.0
27	7.007261625222217E-5	0.0	0.0	5.765014284302823	0.0
28	7.007261625222217E-5	0.0	0.0	5.934239652551939	0.0
29	1.4014523250444435E-4	0.0	0.0	6.115937946493951	0.0
30	2.1021784875666652E-4	0.0	0.0	6.338488575711009	0.0
31	2.1021784875666652E-4	0.0	0.0	6.5321692870321515	0.0
32	2.1021784875666652E-4	0.0	0.0	6.734959438466082	0.0
33	2.802904650088887E-4	0.0	0.0	6.932213853216088	0.0
34	2.802904650088887E-4	0.0	0.0	7.129328122733589	0.0
35	2.802904650088887E-4	0.0	0.0	7.335762050212636	0.0
36	2.802904650088887E-4	0.0	0.0	7.526079275953671	0.0
37	2.802904650088887E-4	0.0	0.0	7.716396501694706	0.0
38	2.802904650088887E-4	0.0	0.0	7.924372026731302	0.0
39	2.802904650088887E-4	0.0	0.0	8.183991069945785	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAC	25	3.8912804E-5	45.000004	14
GTCGGTA	25	3.8912804E-5	45.000004	1
ACGCGTC	20	7.0338603E-4	45.0	15
CGAATAT	225	0.0	41.0	14
CATGACG	50	1.0822987E-9	40.500004	1
TTAACGG	100	0.0	40.500004	2
CGTAAGG	205	0.0	39.512196	2
GCGTACG	40	3.4590084E-7	39.375	1
CATCGTT	155	0.0	39.19355	36
TACGAAT	230	0.0	39.130432	12
GCGTAAG	150	0.0	39.0	1
ACACGAC	665	0.0	38.571426	26
ATAACGC	165	0.0	38.181816	11
CAATGCG	65	9.094947E-12	38.07692	1
CGGCATC	160	0.0	37.96875	33
CGTATGG	155	0.0	37.74194	2
CGAGCAT	60	1.5643309E-10	37.500004	6
TATCGAG	60	1.5643309E-10	37.500004	1
GCGAGAC	690	0.0	37.5	21
GCTACGA	255	0.0	37.058823	10
>>END_MODULE