FastQCFastQC Report
Sat 18 Jun 2016
SRR3553682_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553682_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597707
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10050.16814258491200537No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC9490.15877344585223194No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT8140.1361871284759924No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCG6070.10155477516575848No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5980.10004902067400917No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCTC453.8380676E-1045.00000445
GCGAAGT302.163746E-645.0000041
AATCTCC253.8885504E-545.00000424
GATCTCG253.8885504E-545.00000413
ATTACGG207.0305663E-445.02
TGCGACC207.0305663E-445.013
CCGACCG406.8048394E-945.015
CGGTCTA207.0305663E-445.031
GCTCGAC207.0305663E-445.021
GGTTAGT207.0305663E-445.09
GCGTATG551.8189894E-1245.01
TACACCG207.0305663E-445.01
AAGCTAC850.042.3529438
CTAACGG603.6379788E-1241.2500042
ACGTAGG603.6379788E-1241.2500042
CGTTTAT603.6379788E-1241.25000439
GCGAATG556.002665E-1140.9090921
CGTAAGG1050.040.7142872
ATGGTCA3050.040.57377213
CGGTAGT501.0804797E-940.50000412