##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553682_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	597707
Sequences flagged as poor quality	0
Sequence length	51
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23507671819135	33.0	31.0	34.0	30.0	34.0
2	32.379580630643446	34.0	31.0	34.0	30.0	34.0
3	32.41620727212497	34.0	31.0	34.0	30.0	34.0
4	35.92652085386318	37.0	35.0	37.0	35.0	37.0
5	35.829851415492875	37.0	35.0	37.0	35.0	37.0
6	35.80082716113414	37.0	35.0	37.0	35.0	37.0
7	35.92156022934314	37.0	35.0	37.0	35.0	37.0
8	35.829332766723496	37.0	35.0	37.0	35.0	37.0
9	37.63324003232353	39.0	38.0	39.0	35.0	39.0
10	37.151248019514576	39.0	37.0	39.0	34.0	39.0
11	37.04380909040717	39.0	37.0	39.0	33.0	39.0
12	36.89921985186722	39.0	37.0	39.0	33.0	39.0
13	36.8338015114429	39.0	37.0	39.0	33.0	39.0
14	38.032427259510094	40.0	37.0	41.0	33.0	41.0
15	38.13262518257273	40.0	37.0	41.0	33.0	41.0
16	38.20808523239648	40.0	37.0	41.0	33.0	41.0
17	38.13934419372703	40.0	37.0	41.0	33.0	41.0
18	38.120644396000046	40.0	37.0	41.0	33.0	41.0
19	38.049060827462284	40.0	37.0	41.0	34.0	41.0
20	37.93431396988826	40.0	36.0	41.0	33.0	41.0
21	37.84417615989105	40.0	36.0	41.0	33.0	41.0
22	37.7964872420768	40.0	36.0	41.0	33.0	41.0
23	37.69766624784384	40.0	36.0	41.0	33.0	41.0
24	37.64751123878422	40.0	36.0	41.0	33.0	41.0
25	37.55548454343014	40.0	35.0	41.0	33.0	41.0
26	37.46614478331357	40.0	35.0	41.0	33.0	41.0
27	37.36215737811336	39.0	35.0	41.0	32.0	41.0
28	37.25915707863552	39.0	35.0	41.0	32.0	41.0
29	37.175823605880474	39.0	35.0	41.0	32.0	41.0
30	37.05430252615412	39.0	35.0	41.0	31.0	41.0
31	36.96765806657777	39.0	35.0	41.0	31.0	41.0
32	36.82111302025909	39.0	35.0	41.0	31.0	41.0
33	36.69386505428245	39.0	35.0	41.0	31.0	41.0
34	36.56026949659281	39.0	35.0	41.0	30.0	41.0
35	36.39105447987057	39.0	35.0	41.0	30.0	41.0
36	36.29391993066837	39.0	35.0	41.0	30.0	41.0
37	36.159829147057	39.0	35.0	41.0	30.0	41.0
38	36.05738263062002	38.0	35.0	41.0	29.0	41.0
39	35.95554176210083	38.0	35.0	41.0	29.0	41.0
40	35.833880145288575	38.0	35.0	40.0	29.0	41.0
41	35.70957341975249	38.0	35.0	40.0	28.0	41.0
42	35.59671042835369	38.0	35.0	40.0	28.0	41.0
43	35.49798981775351	38.0	34.0	40.0	28.0	41.0
44	35.36673319870773	38.0	34.0	40.0	27.0	41.0
45	35.27795725999528	37.0	34.0	40.0	27.0	41.0
46	35.10661578331858	37.0	34.0	40.0	26.0	41.0
47	34.96948170257333	37.0	34.0	40.0	26.0	41.0
48	34.83278429063069	37.0	34.0	40.0	26.0	41.0
49	34.750389404842174	37.0	34.0	40.0	26.0	41.0
50	34.621503512590614	36.0	34.0	40.0	26.0	41.0
51	33.17607289190188	35.0	31.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	4.0
11	5.0
12	5.0
13	17.0
14	16.0
15	48.0
16	93.0
17	163.0
18	309.0
19	550.0
20	885.0
21	1422.0
22	2117.0
23	2839.0
24	3764.0
25	4603.0
26	5675.0
27	6711.0
28	7888.0
29	9205.0
30	11070.0
31	13442.0
32	17383.0
33	24203.0
34	42051.0
35	51129.0
36	50858.0
37	72362.0
38	112175.0
39	156660.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.74065051940165	18.146181992180114	23.68016436146808	13.43300312695016
2	30.129310849630336	25.74605952414812	25.92340394206526	18.201225684156285
3	33.03424587632402	25.05341245794344	25.582099590602088	16.330242075130457
4	27.901630732114562	26.353547808541645	26.446068056756904	19.298753402586886
5	25.491587015042487	31.977038917061368	23.821036059473958	18.710338008422188
6	25.252172050854348	36.84765277970644	23.70308529095359	14.197089878485613
7	83.96036854177716	5.977176108026174	6.5167381342363395	3.5457172159603285
8	86.02040799254485	5.174441657869157	5.413522009948018	3.391628339637983
9	80.86654497939627	6.837965759142858	7.154006896355572	5.14148236510531
10	43.45205928657352	25.402747500029278	14.800060899403888	16.34513231399331
11	33.297585606325505	26.507134766700073	20.318985723774357	19.87629390320006
12	27.918528643633085	23.20986704187838	27.469646499037154	21.401957815451382
13	25.77567269581919	24.31325047222134	28.227208314441693	21.68386851751778
14	22.28215496890617	27.90514415926198	26.725636474058362	23.087064397773492
15	22.2809838265237	25.610876231999956	28.94695896149786	23.161180979978486
16	24.3303156897945	22.804819083597817	29.86162116220824	23.003244064399446
17	23.810328471977073	23.169211670601147	26.971074456213493	26.049385401208287
18	24.727834875616313	24.57909979304241	26.473840861826947	24.219224469514327
19	26.43904120246208	26.057416091830948	23.459320369344848	24.044222336362132
20	27.932582352222745	23.77987877003281	24.70742353695038	23.580115340794066
21	26.68431187856257	25.4054243969035	25.199637949697763	22.710625774836167
22	25.375978531287068	23.100783494253875	24.892464033380904	26.630773941078157
23	25.316584881890293	26.98027628921863	23.888627705548036	23.814511123343042
24	23.899669905154198	25.757603641918198	27.163476419048127	23.179250033879477
25	25.45210278614773	25.41094549670658	25.159317190529805	23.977634526615883
26	24.172880692379376	26.097067626780344	25.104775416717555	24.62527626412272
27	24.7833804857564	25.24598172683271	25.99651668794242	23.97412109946847
28	23.25872040983291	26.597480036205027	27.207645217472777	22.936154336489285
29	24.444920337222083	26.458783316909457	25.203653295009094	23.892643050859366
30	24.314923532767725	25.545459564636186	26.00638774516611	24.133229157429977
31	26.413276070047697	25.819841494243832	23.312927571535884	24.45395486417258
32	26.33347108198499	26.432181654221885	23.956386657676752	23.277960606116373
33	26.16081123359773	25.688506241352364	22.74057355861651	25.410108966433388
34	24.316596593314117	26.447406505194017	24.506488965329165	24.7295079361627
35	25.679639020456513	24.254860659152396	25.165340208496804	24.90016011189429
36	26.33029226694685	26.706730889884174	24.029499403553913	22.933477439615064
37	24.614066758461924	27.273731109055106	24.649870254154628	23.462331878328342
38	24.970763266951867	26.543272874502055	25.156640293655585	23.32932356489049
39	24.848797153120174	25.045716379430054	24.79609574590895	25.30939072154082
40	24.447429928041668	25.14944613330612	27.175522454982122	23.227601483670092
41	22.63383229575695	25.429349162716854	26.73065565569752	25.206162885828675
42	23.520888997451927	26.129190389270995	25.45277201036628	24.89714860291079
43	24.122186957823814	24.563205717851723	25.873546737782892	25.441060586541564
44	23.75996934953079	24.86653159491189	26.007558887548583	25.365940168008738
45	25.216033943052363	25.320767533256262	24.850972131830478	24.612226391860894
46	24.86469122831086	25.58544571169486	25.672277554052403	23.877585505941877
47	23.98081334165402	26.305865582969584	26.57087837351746	23.142442701858936
48	24.543630909458983	25.838245160254104	25.645508585310196	23.97261534497672
49	24.040876215269353	26.040016262148512	25.88107551024164	24.038032012340494
50	23.68685660365363	25.081352569068123	26.772147557247948	24.4596432700303
51	23.656741513818645	24.845785644136676	25.40375133635711	26.09372150568757
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1066.0
1	1256.0
2	1446.0
3	1905.5
4	2365.0
5	1851.0
6	1337.0
7	1483.0
8	1629.0
9	1757.0
10	1885.0
11	1790.0
12	1695.0
13	1656.0
14	1617.0
15	1614.5
16	1612.0
17	1550.5
18	1489.0
19	1506.5
20	1524.0
21	1608.0
22	1692.0
23	1987.0
24	2282.0
25	2783.5
26	3638.5
27	3992.0
28	5110.5
29	6229.0
30	7429.0
31	8629.0
32	9830.0
33	11031.0
34	12366.5
35	13702.0
36	14518.5
37	15335.0
38	15926.5
39	16518.0
40	19053.0
41	21588.0
42	24007.5
43	26427.0
44	28938.0
45	31449.0
46	33879.0
47	36309.0
48	38715.0
49	41121.0
50	41414.0
51	41707.0
52	40043.5
53	38380.0
54	37004.0
55	35628.0
56	35262.0
57	34896.0
58	33167.0
59	31438.0
60	31618.0
61	31798.0
62	30561.0
63	29324.0
64	25601.5
65	21879.0
66	20008.0
67	18137.0
68	16226.0
69	14315.0
70	13602.0
71	12889.0
72	11964.5
73	11040.0
74	9237.0
75	6077.0
76	4720.0
77	3904.0
78	3088.0
79	2484.0
80	1880.0
81	1417.0
82	954.0
83	705.5
84	457.0
85	335.5
86	214.0
87	170.5
88	127.0
89	100.0
90	73.0
91	52.5
92	32.0
93	29.0
94	26.0
95	19.5
96	13.0
97	8.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	597707.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.868072856495772
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.12624627947697	17.93118719633601
2	7.333042155515228	3.5005116885518452
3	2.716767174265323	1.945319905485026
4	1.55427633704708	1.4839032340706833
5	1.0821468187739223	1.2914379555960551
6	0.8886029767992649	1.2725544354446325
7	0.7046528324880043	1.1773093601051858
8	0.6366323538196418	1.2156149923015682
9	0.4929969700845009	1.059019884000768
>10	8.267147159699196	49.06791413777872
>50	1.1377199695866094	16.7692486541348
>100	0.054846821772407614	2.4448056401291236
>500	0.0042189862901852015	0.6725019702064527
>1k	7.031643816975335E-4	0.1686709458591178
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1005	0.16814258491200537	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	949	0.15877344585223194	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	814	0.1361871284759924	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCG	607	0.10155477516575848	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	598	0.10004902067400917	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.673060546388113E-4	0.0	0.0	0.02777280507004268	0.0
2	1.673060546388113E-4	0.0	0.0	0.14070439195124032	0.0
3	1.673060546388113E-4	0.0	0.0	0.19691922630988093	0.0
4	1.673060546388113E-4	0.0	0.0	0.27990302941073136	0.0
5	1.673060546388113E-4	0.0	0.0	0.591426903148198	0.0
6	1.673060546388113E-4	0.0	0.0	0.877018338416649	0.0
7	1.673060546388113E-4	0.0	0.0	1.1202813418614805	0.0
8	1.673060546388113E-4	0.0	0.0	1.5444021903708673	0.0
9	1.673060546388113E-4	0.0	0.0	1.755709737379686	0.0
10	1.673060546388113E-4	0.0	0.0	2.2582971255146753	0.0
11	1.673060546388113E-4	0.0	0.0	2.7183887757714063	0.0
12	1.673060546388113E-4	0.0	0.0	3.1393308092426557	0.0
13	1.673060546388113E-4	0.0	0.0	3.2773583043196752	0.0
14	1.673060546388113E-4	0.0	0.0	3.3400980748092293	0.0
15	1.673060546388113E-4	0.0	0.0	3.4319490988059367	0.0
16	1.673060546388113E-4	0.0	0.0	3.6276971827333457	0.0
17	1.673060546388113E-4	0.0	0.0	3.8833408342214497	0.0
18	1.673060546388113E-4	0.0	0.0	4.219626004045461	0.0
19	3.346121092776226E-4	0.0	0.0	4.3790686741162475	0.0
20	3.346121092776226E-4	0.0	0.0	4.524792247706652	0.0
21	3.346121092776226E-4	0.0	0.0	4.701969359569153	0.0
22	3.346121092776226E-4	0.0	0.0	4.883663734906903	0.0
23	3.346121092776226E-4	0.0	0.0	5.075898391686897	0.0
24	3.346121092776226E-4	0.0	0.0	5.227477677189659	0.0
25	3.346121092776226E-4	0.0	0.0	5.3743723931625365	0.0
26	3.346121092776226E-4	0.0	0.0	5.508886461092141	0.0
27	3.346121092776226E-4	0.0	0.0	5.649590853043381	0.0
28	3.346121092776226E-4	0.0	0.0	5.799162465890478	0.0
29	3.346121092776226E-4	0.0	0.0	5.951912893775713	0.0
30	3.346121092776226E-4	0.0	0.0	6.127082332982548	0.0
31	3.346121092776226E-4	0.0	0.0	6.288198063599723	0.0
32	3.346121092776226E-4	0.0	0.0	6.446469591288039	0.0
33	3.346121092776226E-4	0.0	0.0	6.61243719748974	0.0
34	3.346121092776226E-4	0.0	0.0	6.77991055818319	0.0
35	3.346121092776226E-4	0.0	0.0	6.966122196996187	0.0
36	3.346121092776226E-4	0.0	0.0	7.129245600269028	0.0
37	3.346121092776226E-4	0.0	0.0	7.299897776000615	0.0
38	3.346121092776226E-4	0.0	0.0	7.454823182596155	0.0
39	3.346121092776226E-4	0.0	0.0	7.622129237234966	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCCTC	45	3.8380676E-10	45.000004	45
GCGAAGT	30	2.163746E-6	45.000004	1
AATCTCC	25	3.8885504E-5	45.000004	24
GATCTCG	25	3.8885504E-5	45.000004	13
ATTACGG	20	7.0305663E-4	45.0	2
TGCGACC	20	7.0305663E-4	45.0	13
CCGACCG	40	6.8048394E-9	45.0	15
CGGTCTA	20	7.0305663E-4	45.0	31
GCTCGAC	20	7.0305663E-4	45.0	21
GGTTAGT	20	7.0305663E-4	45.0	9
GCGTATG	55	1.8189894E-12	45.0	1
TACACCG	20	7.0305663E-4	45.0	1
AAGCTAC	85	0.0	42.352943	8
CTAACGG	60	3.6379788E-12	41.250004	2
ACGTAGG	60	3.6379788E-12	41.250004	2
CGTTTAT	60	3.6379788E-12	41.250004	39
GCGAATG	55	6.002665E-11	40.909092	1
CGTAAGG	105	0.0	40.714287	2
ATGGTCA	305	0.0	40.573772	13
CGGTAGT	50	1.0804797E-9	40.500004	12
>>END_MODULE