SRR3553678_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553678_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	562041
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4214	0.749767365725988	No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	2248	0.3999708206340819	TruSeq Adapter, Index 22 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1993	0.35460046509062504	No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	1677	0.29837680880932177	Illumina Paired End PCR Primer 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	1524	0.27115459548324766	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCC	1205	0.21439717031319777	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1045	0.1859294962467151	No Hit
TCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	995	0.17703334810093924	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCG	718	0.12774868737334108	No Hit
ACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	698	0.12419022811503075	No Hit
AAAACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC	649	0.11547200293217043	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	614	0.10924469923012735	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC	596	0.10604208589764803	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATC	20	7.030205E-4	45.000004	23
GATCGAC	25	3.8882514E-5	45.000004	9
CGAAACA	20	7.030205E-4	45.000004	43
TCCGAAT	25	3.8882514E-5	45.000004	14
TTTCGTT	25	3.8882514E-5	45.000004	28
GACAATC	20	7.030205E-4	45.000004	10
TTCGTTA	25	3.8882514E-5	45.000004	29
TAACGCA	20	7.030205E-4	45.000004	16
CGTTAGT	25	3.8882514E-5	45.000004	31
ATCCGGT	25	3.8882514E-5	45.000004	18
AATGCGA	20	7.030205E-4	45.000004	45
CCCGTAA	20	7.030205E-4	45.000004	27
TACGCGG	50	2.1827873E-11	45.000004	2
CCGTAAT	20	7.030205E-4	45.000004	28
CGTAGTG	25	3.8882514E-5	45.000004	28
TTACGCG	20	7.030205E-4	45.000004	1
TACTAAC	20	7.030205E-4	45.000004	13
ACGTATG	20	7.030205E-4	45.000004	1
ACCCGTC	25	3.8882514E-5	45.000004	3
ATAGTAG	20	7.030205E-4	45.000004	1