##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553678_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 562041 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.90825580340224 33.0 31.0 34.0 30.0 34.0 2 32.03743143293816 33.0 31.0 34.0 30.0 34.0 3 32.078085050734735 33.0 31.0 34.0 30.0 34.0 4 35.66078453351268 37.0 35.0 37.0 33.0 37.0 5 35.57953957095657 37.0 35.0 37.0 33.0 37.0 6 35.543424767943975 37.0 35.0 37.0 33.0 37.0 7 35.72055419444489 37.0 35.0 37.0 35.0 37.0 8 35.587732923398825 37.0 35.0 37.0 35.0 37.0 9 37.43479390293591 39.0 37.0 39.0 35.0 39.0 10 36.850699504128706 39.0 37.0 39.0 32.0 39.0 11 36.79580493238038 39.0 37.0 39.0 32.0 39.0 12 36.68454258675079 39.0 35.0 39.0 33.0 39.0 13 36.5566444440886 39.0 35.0 39.0 32.0 39.0 14 37.64547248332417 39.0 36.0 41.0 33.0 41.0 15 37.76293722344099 40.0 36.0 41.0 33.0 41.0 16 37.86723566430207 40.0 36.0 41.0 33.0 41.0 17 37.79330689398105 39.0 36.0 41.0 33.0 41.0 18 37.72982398081279 39.0 36.0 41.0 33.0 41.0 19 37.60007543933628 39.0 36.0 41.0 33.0 41.0 20 37.55497018900756 39.0 35.0 41.0 33.0 41.0 21 37.4673022074902 39.0 35.0 41.0 33.0 41.0 22 37.412720780156604 39.0 35.0 41.0 33.0 41.0 23 37.32072393295151 39.0 35.0 41.0 32.0 41.0 24 37.32500120098 39.0 35.0 41.0 32.0 41.0 25 37.23534404073724 39.0 35.0 41.0 32.0 41.0 26 37.18595974315041 39.0 35.0 41.0 32.0 41.0 27 37.136251625771074 39.0 35.0 41.0 32.0 41.0 28 37.04534188786939 39.0 35.0 41.0 32.0 41.0 29 36.96586903802392 39.0 35.0 41.0 32.0 41.0 30 36.845913376426275 39.0 35.0 41.0 31.0 41.0 31 36.65514437558826 39.0 35.0 40.0 31.0 41.0 32 36.51428454507767 39.0 35.0 40.0 31.0 41.0 33 36.42163294136905 39.0 35.0 40.0 30.0 41.0 34 36.26888607770608 38.0 35.0 40.0 30.0 41.0 35 36.126086886899714 38.0 35.0 40.0 30.0 41.0 36 36.01678703155108 38.0 35.0 40.0 30.0 41.0 37 35.88264557212018 38.0 35.0 40.0 29.0 41.0 38 35.795651918632274 38.0 35.0 40.0 29.0 41.0 39 35.68555318918015 38.0 35.0 40.0 29.0 41.0 40 35.54678573271345 38.0 34.0 40.0 28.0 41.0 41 35.41916337064378 38.0 34.0 40.0 27.0 41.0 42 35.37392111963362 38.0 34.0 40.0 27.0 41.0 43 35.27958992315507 38.0 34.0 40.0 27.0 41.0 44 35.156716324965615 38.0 34.0 40.0 26.0 41.0 45 35.038310372374966 37.0 34.0 40.0 26.0 41.0 46 34.86909495926454 37.0 34.0 40.0 26.0 41.0 47 34.7099606612329 37.0 34.0 40.0 25.0 41.0 48 34.6117756533776 37.0 33.0 40.0 25.0 41.0 49 34.589713205976075 37.0 34.0 40.0 25.0 41.0 50 34.462350611432264 36.0 33.0 40.0 24.0 41.0 51 32.948347184636 35.0 31.0 39.0 21.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 6.0 12 8.0 13 9.0 14 10.0 15 38.0 16 68.0 17 154.0 18 283.0 19 517.0 20 862.0 21 1302.0 22 2050.0 23 2769.0 24 3695.0 25 4675.0 26 5571.0 27 6624.0 28 8006.0 29 9464.0 30 11768.0 31 14975.0 32 19150.0 33 26176.0 34 42631.0 35 54479.0 36 50185.0 37 70218.0 38 107802.0 39 118513.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.68232744586249 19.833428522118492 24.51191283198201 10.972331200037008 2 31.33454676794042 25.92622246419745 24.888753667437072 17.850477100425056 3 31.214448767972442 26.725808259539786 26.030663243428858 16.02907972905891 4 26.749294090644632 28.34152668577559 26.042050313055455 18.867128910524322 5 23.674785291464502 32.67946644461881 24.561197492709606 19.08455077120708 6 22.972167510911127 38.1970354475919 24.073866497284005 14.756930544212969 7 78.79193866639622 8.415578222941031 8.165240614118899 4.627242496543847 8 79.36200383957754 7.413160249875009 7.704775986093542 5.52005992445391 9 74.18480146466183 9.165879357555767 10.26419780763325 6.385121370149152 10 37.98192658542704 26.611403794385108 19.118534057123945 16.288135563063904 11 30.50133353260705 27.786940810367927 21.522984977964242 20.18874067906078 12 26.375477945559133 23.719977724045044 29.78199099353962 20.122553336856207 13 23.459854352262557 27.606526925971593 30.23943093119541 18.69418779057044 14 19.77400225250471 30.693668255518723 27.927499951071184 21.604829540905378 15 18.681911106129267 27.392129755658395 33.57228387252887 20.353675265683467 16 20.599386877469794 26.354838881860932 31.566202465656417 21.479571775012854 17 20.161874311660537 26.225310964858433 27.394442754176296 26.218371969304734 18 22.887298257600424 25.630158653906033 29.62488501728522 21.857658071208327 19 22.646034719886984 28.687053079757526 26.640761083266167 22.026151117089324 20 24.51778428975822 27.12328815869305 26.94073919874173 21.418188352807004 21 22.970922050170717 29.420985301784036 26.44468997813327 21.16340266991198 22 21.128529769180542 26.09898566118842 24.943553940015054 27.82893062961599 23 19.61956512069404 30.573036486662 25.917148393088763 23.890249999555195 24 21.92633633489372 26.57475166402451 28.573182383491595 22.925729617590175 25 20.700625043368724 28.641860647176987 27.007994078723797 23.6495202307305 26 19.840901286560943 28.2819224931989 28.158799802861356 23.718376417378803 27 21.988431448951236 28.241533980617074 26.66264560770478 23.10738896272692 28 19.486478744433235 28.17267779396877 28.481729980553023 23.85911348104498 29 20.899364992945355 27.23591339421857 26.86654532320596 24.998176289630116 30 24.447682642369507 26.603041415128075 26.030663243428858 22.918612699073552 31 24.86740291188721 27.7269807718654 24.113899163939998 23.291717152307395 32 23.819258737351902 28.827434297497867 24.467254168290214 22.886052796860014 33 25.363630055458586 27.670045423732432 23.2447454900977 23.721579030711283 34 21.769230358639316 26.05023476934957 27.29800850827609 24.882526363735032 35 22.287519949612218 25.88618979754146 26.774737074341555 25.051553178504772 36 25.372882049530194 28.263952273944426 25.7399371220249 20.62322855450047 37 23.041913312374007 28.423905017605477 27.661149275586656 20.873032394433856 38 23.472842728555392 30.019518149031832 25.171651178472743 21.335987943940033 39 24.02315845285308 26.888429847644566 26.560339904028353 22.528071795473995 40 23.704498426271392 24.100554941721335 29.913120217208355 22.281826414798918 41 20.060458222798694 25.98226819751584 28.04083687844837 25.9164367012371 42 22.731259819123515 26.049345154534993 26.79982421211264 24.419570814228855 43 23.532268998169172 25.831923293852228 26.9919810120614 23.643826695917202 44 23.43209837004774 25.58727921984339 25.95024206419105 25.030380345917823 45 23.117174725687274 25.36469759323608 26.23224996041214 25.28587772066451 46 24.83217416522994 25.512551575418875 27.30032150679399 22.354952752557196 47 21.416764969103678 25.612188434651568 30.12627192678114 22.844774669463614 48 21.512131677226396 25.932805613825327 28.540800404240972 24.014262304707305 49 22.888187872415003 24.059276814324935 29.9136539860971 23.138881327162967 50 21.99021067858039 23.856444636601246 29.395898164012944 24.75744652080542 51 22.186459706676203 23.839364032161356 26.427431450730467 27.546744810431978 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4667.0 1 4146.0 2 3625.0 3 3369.5 4 3114.0 5 2341.0 6 1568.0 7 1644.5 8 1721.0 9 1859.0 10 1997.0 11 2097.0 12 2197.0 13 2208.5 14 2220.0 15 2200.0 16 2180.0 17 2314.5 18 2449.0 19 2403.0 20 2357.0 21 2425.0 22 2493.0 23 2362.0 24 2231.0 25 2491.5 26 3520.0 27 4288.0 28 4534.5 29 4781.0 30 5995.0 31 7209.0 32 7847.5 33 8486.0 34 9241.0 35 9996.0 36 11556.5 37 13117.0 38 14710.5 39 16304.0 40 17569.5 41 18835.0 42 23833.5 43 28832.0 44 33311.0 45 37790.0 46 43134.5 47 48479.0 48 53849.0 49 59219.0 50 57944.0 51 56669.0 52 50072.0 53 43475.0 54 40563.0 55 37651.0 56 32934.5 57 28218.0 58 25843.5 59 23469.0 60 20906.5 61 18344.0 62 16859.0 63 15374.0 64 13632.5 65 11891.0 66 10145.0 67 8399.0 68 7789.0 69 7179.0 70 6417.0 71 5655.0 72 5006.5 73 4358.0 74 3766.0 75 2652.5 76 2131.0 77 1831.5 78 1532.0 79 1152.0 80 772.0 81 632.0 82 492.0 83 366.5 84 241.0 85 145.5 86 50.0 87 36.5 88 23.0 89 14.0 90 5.0 91 12.0 92 19.0 93 12.0 94 5.0 95 5.5 96 6.0 97 3.5 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 562041.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.55631606284925 #Duplication Level Percentage of deduplicated Percentage of total 1 74.4124282672845 22.73769677139243 2 11.114409090825056 6.792307940621107 3 4.1095235560978205 3.7671570194354755 4 1.9468433684769686 2.3795344516817734 5 1.1840221063754486 1.808967685390436 6 0.7728288086928795 1.4168880804536927 7 0.5791676442864268 1.2388060714534332 8 0.4486127933922853 1.0966363443785887 9 0.3433234392499147 0.9441629569354336 >10 3.978842271088892 28.897082462376456 >50 0.9971867846846607 20.56641769038871 >100 0.10521314258103101 5.107981654442737 >500 0.003507104752701034 0.7626518991737643 >1k 0.004091622211484539 2.483708971875964 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4214 0.749767365725988 No Hit GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC 2248 0.3999708206340819 TruSeq Adapter, Index 22 (95% over 22bp) GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1993 0.35460046509062504 No Hit CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT 1677 0.29837680880932177 Illumina Paired End PCR Primer 2 (95% over 21bp) CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC 1524 0.27115459548324766 TruSeq Adapter, Index 22 (95% over 22bp) CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCC 1205 0.21439717031319777 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1045 0.1859294962467151 No Hit TCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC 995 0.17703334810093924 TruSeq Adapter, Index 22 (95% over 22bp) GAATGATACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCG 718 0.12774868737334108 No Hit ACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC 698 0.12419022811503075 No Hit AAAACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC 649 0.11547200293217043 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 614 0.10924469923012735 No Hit GAATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC 596 0.10604208589764803 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.7792296291551683E-4 0.0 0.0 0.31011972436174584 0.0 2 1.7792296291551683E-4 0.0 0.0 1.331219608533897 0.0 3 1.7792296291551683E-4 0.0 0.0 1.7463138810157979 0.0 4 1.7792296291551683E-4 0.0 0.0 2.318158283826269 0.0 5 1.7792296291551683E-4 0.0 0.0 4.0041562804137065 0.0 6 1.7792296291551683E-4 0.0 0.0 5.054613453466918 0.0 7 1.7792296291551683E-4 0.0 0.0 6.03318974950226 0.0 8 1.7792296291551683E-4 0.0 0.0 7.5937520572342585 0.0 9 1.7792296291551683E-4 0.0 0.0 8.230004572620146 0.0 10 1.7792296291551683E-4 0.0 0.0 9.91066488032012 0.0 11 1.7792296291551683E-4 0.0 0.0 11.757683158346099 0.0 12 1.7792296291551683E-4 0.0 0.0 13.175729172782768 0.0 13 1.7792296291551683E-4 0.0 0.0 13.707362985974333 0.0 14 1.7792296291551683E-4 0.0 0.0 13.91144062443843 0.0 15 1.7792296291551683E-4 0.0 0.0 14.299490606557173 0.0 16 1.7792296291551683E-4 0.0 0.0 15.183233963358544 0.0 17 1.7792296291551683E-4 0.0 0.0 16.2628705023299 0.0 18 1.7792296291551683E-4 0.0 0.0 17.58964203679091 0.0 19 1.7792296291551683E-4 0.0 0.0 18.19653726329574 0.0 20 1.7792296291551683E-4 0.0 0.0 18.739736069076812 0.0 21 1.7792296291551683E-4 0.0 0.0 19.40570171926959 0.0 22 1.7792296291551683E-4 0.0 0.0 20.08483366871812 0.0 23 1.7792296291551683E-4 0.0 0.0 20.834245188518274 0.0 24 1.7792296291551683E-4 0.0 0.0 21.406089591328747 0.0 25 1.7792296291551683E-4 0.0 0.0 21.933987022299085 0.0 26 1.7792296291551683E-4 0.0 0.0 22.397476340694006 0.0 27 1.7792296291551683E-4 0.0 0.0 22.867015039828054 0.0 28 1.7792296291551683E-4 0.0 0.0 23.329436820445483 0.0 29 1.7792296291551683E-4 0.0 0.0 23.805202823281576 0.0 30 3.5584592583103367E-4 0.0 0.0 24.34911332091431 0.0 31 3.5584592583103367E-4 0.0 0.0 24.862243145962662 0.0 32 3.5584592583103367E-4 0.0 0.0 25.341567608057062 0.0 33 3.5584592583103367E-4 0.0 0.0 25.819468686448143 0.0 34 5.337688887465505E-4 0.0 0.0 26.293633382617994 0.0 35 5.337688887465505E-4 0.0 0.0 26.85693748320852 0.0 36 5.337688887465505E-4 0.0 0.0 27.331813871230036 0.0 37 5.337688887465505E-4 0.0 0.0 27.78765250221959 0.0 38 5.337688887465505E-4 0.0 0.0 28.261105506537778 0.0 39 5.337688887465505E-4 0.0 0.0 28.71907921308232 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGATC 20 7.030205E-4 45.000004 23 GATCGAC 25 3.8882514E-5 45.000004 9 CGAAACA 20 7.030205E-4 45.000004 43 TCCGAAT 25 3.8882514E-5 45.000004 14 TTTCGTT 25 3.8882514E-5 45.000004 28 GACAATC 20 7.030205E-4 45.000004 10 TTCGTTA 25 3.8882514E-5 45.000004 29 TAACGCA 20 7.030205E-4 45.000004 16 CGTTAGT 25 3.8882514E-5 45.000004 31 ATCCGGT 25 3.8882514E-5 45.000004 18 AATGCGA 20 7.030205E-4 45.000004 45 CCCGTAA 20 7.030205E-4 45.000004 27 TACGCGG 50 2.1827873E-11 45.000004 2 CCGTAAT 20 7.030205E-4 45.000004 28 CGTAGTG 25 3.8882514E-5 45.000004 28 TTACGCG 20 7.030205E-4 45.000004 1 TACTAAC 20 7.030205E-4 45.000004 13 ACGTATG 20 7.030205E-4 45.000004 1 ACCCGTC 25 3.8882514E-5 45.000004 3 ATAGTAG 20 7.030205E-4 45.000004 1 >>END_MODULE