FastQCFastQC Report
Sat 18 Jun 2016
SRR3553676_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553676_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences582865
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46080.7905775779983357No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC31720.544208350132535TruSeq Adapter, Index 23 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20870.35805889871582613No Hit
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC20130.3453629914302626TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT15470.26541308879414616Illumina Single End Adapter 1 (95% over 22bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC14510.2489427225858475No Hit
AAAACTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC12210.2094824702117986TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCC11940.20485017971571462No Hit
TCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC10310.17688487042454085TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10060.1725957125577964No Hit
ACTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC9670.16590462628567507Illumina Single End Adapter 1 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT7130.12232678235955152No Hit
CGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG6410.10997400770332752No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC6320.10842991087129954No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACA207.0304226E-445.013
CGAGTTA207.0304226E-445.021
CTCCGAT207.0304226E-445.041
TCTCTAT207.0304226E-445.036
CCCTATA253.8884315E-545.040
GCAACCG207.0304226E-445.01
CGTTGTA207.0304226E-445.012
GACCCGT207.0304226E-445.020
GCACCGT253.8884315E-545.024
ATCGTGT207.0304226E-445.020
CGCCTAG207.0304226E-445.015
CCGTAGT253.8884315E-545.027
CCCCGCA351.2105556E-745.029
GCCCTAT253.8884315E-545.039
AACGCCC253.8884315E-545.027
AAATTCG207.0304226E-445.030
AGCGATC207.0304226E-445.019
CGAATGC253.8884315E-545.026
TTAACGG453.8380676E-1045.02
GTATACT253.8884315E-545.013