##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553676_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	582865
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87782248033421	33.0	31.0	34.0	30.0	34.0
2	32.03043071723298	33.0	31.0	34.0	30.0	34.0
3	32.06681993257444	33.0	31.0	34.0	30.0	34.0
4	35.63441277139646	37.0	35.0	37.0	33.0	37.0
5	35.562377222856064	37.0	35.0	37.0	33.0	37.0
6	35.53594228509174	37.0	35.0	37.0	33.0	37.0
7	35.7216010568485	37.0	35.0	37.0	35.0	37.0
8	35.610760639256085	37.0	35.0	37.0	35.0	37.0
9	37.41863038610999	39.0	37.0	39.0	34.0	39.0
10	36.8698274900706	39.0	37.0	39.0	32.0	39.0
11	36.80626903313804	39.0	37.0	39.0	33.0	39.0
12	36.74262479304813	39.0	35.0	39.0	33.0	39.0
13	36.644282981479414	39.0	35.0	39.0	32.0	39.0
14	37.692788209962856	40.0	36.0	41.0	33.0	41.0
15	37.807619260034485	40.0	36.0	41.0	33.0	41.0
16	37.92031774081477	40.0	36.0	41.0	33.0	41.0
17	37.84772631741484	39.0	36.0	41.0	33.0	41.0
18	37.799684317981004	39.0	36.0	41.0	33.0	41.0
19	37.67360194899334	39.0	36.0	41.0	33.0	41.0
20	37.59971863124394	39.0	35.0	41.0	33.0	41.0
21	37.4957511602172	39.0	35.0	41.0	33.0	41.0
22	37.460748200698276	39.0	35.0	41.0	33.0	41.0
23	37.38659209250856	39.0	35.0	41.0	33.0	41.0
24	37.36094635979172	39.0	35.0	41.0	33.0	41.0
25	37.26397364741407	39.0	35.0	41.0	32.0	41.0
26	37.22740943443164	39.0	35.0	41.0	32.0	41.0
27	37.1867773841284	39.0	35.0	41.0	32.0	41.0
28	37.11300901580984	39.0	35.0	41.0	32.0	41.0
29	37.02988685201547	39.0	35.0	41.0	32.0	41.0
30	36.921271649524336	39.0	35.0	41.0	31.0	41.0
31	36.804872483336624	39.0	35.0	41.0	31.0	41.0
32	36.69137107220369	39.0	35.0	41.0	31.0	41.0
33	36.592797646110164	39.0	35.0	41.0	31.0	41.0
34	36.499251113036465	39.0	35.0	41.0	30.0	41.0
35	36.36102871162276	39.0	35.0	41.0	30.0	41.0
36	36.26163176721882	39.0	35.0	40.0	30.0	41.0
37	36.15644445969478	38.0	35.0	40.0	30.0	41.0
38	36.043883231966234	38.0	35.0	40.0	30.0	41.0
39	35.994641983992864	38.0	35.0	40.0	30.0	41.0
40	35.82383570809707	38.0	35.0	40.0	29.0	41.0
41	35.68370892059053	38.0	35.0	40.0	29.0	41.0
42	35.56822934984945	38.0	35.0	40.0	28.0	41.0
43	35.4977533391094	38.0	35.0	40.0	28.0	41.0
44	35.367795287073335	38.0	34.0	40.0	27.0	41.0
45	35.284112101430004	38.0	34.0	40.0	27.0	41.0
46	35.133030804731796	38.0	34.0	40.0	26.0	41.0
47	35.00433033378226	37.0	34.0	40.0	26.0	41.0
48	34.87632642207029	37.0	34.0	40.0	26.0	41.0
49	34.84936306005679	37.0	34.0	40.0	26.0	41.0
50	34.77947037478661	37.0	34.0	40.0	26.0	41.0
51	33.323550050183144	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	3.0
11	1.0
12	8.0
13	5.0
14	10.0
15	29.0
16	73.0
17	147.0
18	309.0
19	523.0
20	894.0
21	1338.0
22	1971.0
23	2841.0
24	3795.0
25	4729.0
26	5698.0
27	6659.0
28	7968.0
29	9425.0
30	11680.0
31	14556.0
32	18736.0
33	25714.0
34	42267.0
35	54644.0
36	50703.0
37	70954.0
38	111145.0
39	135988.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.25946831599084	22.6380036543625	24.50859118320709	11.59393684643957
2	30.40343818894598	27.579799782110783	24.203203143094886	17.81355888584835
3	29.995796625290588	26.677532533262422	26.587631784375453	16.739039057071533
4	26.95272490199274	28.670275278151884	25.707325023804827	18.669674796050543
5	22.543642181294125	32.035033841455565	25.252845856244583	20.168478121005723
6	22.209602566632068	36.799430399835295	26.004477880812882	14.986489152719756
7	78.13284379744881	8.289054926955641	8.487385586713904	5.090715688881645
8	78.3383802424232	6.807236667152772	8.67679479810934	6.177588292314687
9	72.47784649961827	9.998026987381298	10.955881722182667	6.56824479081777
10	36.64879517555523	26.397707874036016	19.79995367709504	17.15354327331372
11	28.51981162018649	28.379298808471944	22.730134765340175	20.37075480600139
12	25.41960831410361	23.14240861949165	30.626302831702024	20.811680234702717
13	23.24294647988814	27.7959733385947	30.55132835219133	18.40975182932583
14	18.35622313914886	31.79501256723255	29.11737709418133	20.73138719943726
15	17.621233047103534	28.943923550050183	33.28815420380363	20.14668919904266
16	19.38373379770616	26.075849467715507	32.03348974462354	22.506926989954792
17	18.56296054832594	26.59844046219965	27.88844758220171	26.950151407272692
18	21.375275578392937	26.81118269239018	28.625324903708403	23.18821682550848
19	21.341648580717663	28.675936966535993	26.86900054043389	23.113413912312456
20	22.338277302634403	28.30535372684927	27.396395391728788	21.959973578787544
21	21.739682430751547	29.509749255831107	28.095871256637473	20.654697056779874
22	20.297667555952064	27.15585941856176	24.85790019987476	27.68857282561142
23	18.767467595412317	29.71974642498692	26.164720818714454	25.348065160886314
24	20.52447822394551	27.291053674521542	28.68451528226948	23.499952819263466
25	20.07634701002805	28.994192480248426	26.465819700959912	24.463640808763607
26	18.341811568716597	29.78168186458271	27.834575759395403	24.04193080730529
27	20.78663155276093	28.36625976855704	28.14322355948633	22.703885119195697
28	18.11791752807254	27.9430056702667	29.160611805478116	24.77846499618265
29	19.495423468556183	26.709958566735008	29.618179166702408	24.1764387980064
30	20.474037727432595	26.031585358530705	29.391540065023637	24.102836849013066
31	20.959227265318727	27.822222984739177	26.42927607593525	24.789273674006846
32	21.45333825156769	26.5771662391806	28.130184519571426	23.83931098968029
33	20.436636270834583	25.253875254132602	25.91028797405917	28.399200500973638
34	18.61906273322296	24.90885539533168	29.582664939565767	26.889416931879595
35	20.466317243272456	23.641323462551362	30.532284491262985	25.360074802913196
36	22.402614670635568	24.71550015869884	27.78945381863725	25.092431352028342
37	19.809904523345885	25.786245528552925	30.20562222813173	24.198227719969463
38	20.608202585504362	26.996645878548204	26.669812049102283	25.72533948684515
39	21.481303560858862	22.896554090569857	28.378955675842604	27.243186672728676
40	23.308999511036003	22.96638158064046	30.80027107477718	22.92434783354636
41	20.552615099551357	24.749813421632798	27.785507793399844	26.912063685416005
42	21.110720321172142	24.121022878368063	28.874953891552934	25.89330290890687
43	22.203940878247966	22.85520660873444	28.77630326061781	26.164549252399784
44	20.677172244001614	23.51951137913582	27.979377728976694	27.823938647885875
45	21.810539318710166	23.19851080438867	27.754797423073953	27.236152453827216
46	23.030547382326954	23.586079109227693	28.241702624106786	25.14167088433857
47	20.681289835553688	23.41228243246721	31.198648057440405	24.707779674538703
48	20.94035497070505	23.097801377677506	29.56516517546945	26.396678476147994
49	21.603801909533082	21.85343089737761	30.810736619972033	25.732030573117274
50	20.290976469679944	21.502063084933905	30.724095631063797	27.482864814322355
51	19.70559220402666	21.988110454393382	27.96256423013905	30.3437331114409
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5147.0
1	4608.5
2	4070.0
3	3617.5
4	3165.0
5	2492.5
6	1820.0
7	1820.0
8	1820.0
9	2000.0
10	2180.0
11	2256.0
12	2332.0
13	2427.0
14	2522.0
15	2636.0
16	2750.0
17	2648.0
18	2546.0
19	2496.0
20	2446.0
21	2381.5
22	2317.0
23	2511.5
24	2706.0
25	2775.0
26	3287.5
27	3731.0
28	4377.0
29	5023.0
30	6320.5
31	7618.0
32	8249.5
33	8881.0
34	9674.5
35	10468.0
36	11661.5
37	12855.0
38	14166.5
39	15478.0
40	18217.0
41	20956.0
42	26314.0
43	31672.0
44	36394.0
45	41116.0
46	47166.5
47	53217.0
48	60457.0
49	67697.0
50	65483.5
51	63270.0
52	54418.5
53	45567.0
54	38722.5
55	31878.0
56	27750.0
57	23622.0
58	21639.5
59	19657.0
60	19477.5
61	19298.0
62	17500.0
63	15702.0
64	13707.0
65	11712.0
66	10059.0
67	8406.0
68	7794.5
69	7183.0
70	6617.5
71	6052.0
72	5619.5
73	5187.0
74	4224.0
75	2589.0
76	1917.0
77	1539.5
78	1162.0
79	920.0
80	678.0
81	569.0
82	460.0
83	337.0
84	214.0
85	169.5
86	125.0
87	105.5
88	86.0
89	56.0
90	26.0
91	19.0
92	12.0
93	8.5
94	5.0
95	5.0
96	5.0
97	4.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	582865.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.144094661426625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.01955741066965	22.011084507227018
2	11.649622925690602	7.023346724838866
3	4.484120507053191	4.055092591135673
4	2.286055979863575	2.75644351433312
5	1.319274521929289	1.988416803672242
6	0.892406992442063	1.6140480514015547
7	0.6157430426141818	1.299271159457372
8	0.47423970796418813	1.1436421319263845
9	0.37760657310195744	1.0244347455926086
>10	3.745350897726181	26.434157962228078
>50	0.9890136311148175	20.256232797831792
>100	0.1378555726423776	6.370722813012793
>500	0.003432088945453384	0.6900596177680786
>1k	0.0057201482424223075	3.3330465795744524
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4608	0.7905775779983357	No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	3172	0.544208350132535	TruSeq Adapter, Index 23 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2087	0.35805889871582613	No Hit
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	2013	0.3453629914302626	TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT	1547	0.26541308879414616	Illumina Single End Adapter 1 (95% over 22bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1451	0.2489427225858475	No Hit
AAAACTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC	1221	0.2094824702117986	TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCC	1194	0.20485017971571462	No Hit
TCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	1031	0.17688487042454085	TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1006	0.1725957125577964	No Hit
ACTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	967	0.16590462628567507	Illumina Single End Adapter 1 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT	713	0.12232678235955152	No Hit
CGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG	641	0.10997400770332752	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC	632	0.10842991087129954	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2727904403249466	0.0
2	0.0	0.0	0.0	1.5382635773292272	0.0
3	0.0	0.0	0.0	2.13823097972944	0.0
4	0.0	0.0	0.0	2.8500596192943477	0.0
5	0.0	0.0	0.0	4.9906925274291645	0.0
6	0.0	0.0	0.0	6.4061146234548305	0.0
7	0.0	0.0	0.0	7.631098110197044	0.0
8	0.0	0.0	0.0	9.29340413303252	0.0
9	0.0	0.0	0.0	9.962512760244653	0.0
10	0.0	0.0	0.0	11.640088185085741	0.0
11	0.0	0.0	0.0	13.891038233553225	0.0
12	0.0	0.0	0.0	15.506335086169182	0.0
13	0.0	0.0	0.0	16.142502980964718	0.0
14	0.0	0.0	0.0	16.377548832062313	0.0
15	0.0	0.0	0.0	16.762372075866626	0.0
16	1.7156631466977773E-4	0.0	0.0	17.77495646504765	0.0
17	1.7156631466977773E-4	0.0	0.0	18.9872440445043	0.0
18	1.7156631466977773E-4	0.0	0.0	20.43286181191185	0.0
19	1.7156631466977773E-4	0.0	0.0	21.06679934461668	0.0
20	1.7156631466977773E-4	0.0	0.0	21.66059035968878	0.0
21	1.7156631466977773E-4	0.0	0.0	22.416168409494478	0.0
22	1.7156631466977773E-4	0.0	0.0	23.21172141061824	0.0
23	0.001200964202688444	0.0	0.0	23.971588618290685	0.0
24	0.001200964202688444	0.0	0.0	24.60930060991825	0.0
25	0.001200964202688444	0.0	0.0	25.14801883798135	0.0
26	0.001200964202688444	0.0	0.0	25.63406620744083	0.0
27	0.001200964202688444	0.0	0.0	26.124231168452386	0.0
28	0.001200964202688444	0.0	0.0	26.640474209293746	0.0
29	0.001200964202688444	0.0	0.0	27.143163511276196	0.0
30	0.001200964202688444	0.0	0.0	27.725802715894762	0.0
31	0.0013725305173582219	0.0	0.0	28.279275647019464	0.0
32	0.0013725305173582219	0.0	0.0	28.80615579937035	0.0
33	0.0013725305173582219	0.0	0.0	29.281566057320305	0.0
34	0.0013725305173582219	0.0	0.0	29.74805486690743	0.0
35	0.0013725305173582219	0.0	0.0	30.259322484623368	0.0
36	0.0013725305173582219	0.0	0.0	30.75051684352294	0.0
37	0.0013725305173582219	0.0	0.0	31.218892882571435	0.0
38	0.0013725305173582219	0.0	0.0	31.699450129961484	0.0
39	0.0013725305173582219	0.0	0.0	32.16645363849262	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACA	20	7.0304226E-4	45.0	13
CGAGTTA	20	7.0304226E-4	45.0	21
CTCCGAT	20	7.0304226E-4	45.0	41
TCTCTAT	20	7.0304226E-4	45.0	36
CCCTATA	25	3.8884315E-5	45.0	40
GCAACCG	20	7.0304226E-4	45.0	1
CGTTGTA	20	7.0304226E-4	45.0	12
GACCCGT	20	7.0304226E-4	45.0	20
GCACCGT	25	3.8884315E-5	45.0	24
ATCGTGT	20	7.0304226E-4	45.0	20
CGCCTAG	20	7.0304226E-4	45.0	15
CCGTAGT	25	3.8884315E-5	45.0	27
CCCCGCA	35	1.2105556E-7	45.0	29
GCCCTAT	25	3.8884315E-5	45.0	39
AACGCCC	25	3.8884315E-5	45.0	27
AAATTCG	20	7.0304226E-4	45.0	30
AGCGATC	20	7.0304226E-4	45.0	19
CGAATGC	25	3.8884315E-5	45.0	26
TTAACGG	45	3.8380676E-10	45.0	2
GTATACT	25	3.8884315E-5	45.0	13
>>END_MODULE