FastQCFastQC Report
Sat 18 Jun 2016
SRR3553675_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553675_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945598
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31430.3323822596917506No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29840.3155675033153623No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCC18180.192259289888515No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13400.141709267574593No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC12310.13018217043606267Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC9570.10120579781260113Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG207.032651E-445.0000041
GTGGCGT207.032651E-445.00000413
CGATCTA207.032651E-445.00000410
GACGTTG302.1650922E-644.9999961
ACGGGTC1700.041.029415
TCGATAG556.002665E-1140.9090921
TCGTAAG403.4573532E-739.3750041
TCATCGA403.4573532E-739.37500416
GCTACGA403.4573532E-739.37500410
GCTAGCG1150.039.1304321
GTACGAG750.039.01
CTACGAA356.2471154E-638.57142611
TCACCGC1000.038.2524
ACCGCTA1000.038.2526
TACGGGT2000.038.254
CGCTACA1000.038.2528
GCGTAAG950.037.8947371
CGGGATA1250.037.8000036
AACGGGA3650.037.6027374
ACGGGAA4200.037.55
Produced by FastQC (version 0.11.5)