##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553675_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	945598
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.106021797846445	33.0	31.0	34.0	30.0	34.0
2	32.251701039976815	34.0	31.0	34.0	30.0	34.0
3	32.26765390789743	34.0	31.0	34.0	30.0	34.0
4	35.811896810272444	37.0	35.0	37.0	35.0	37.0
5	35.747669728573875	37.0	35.0	37.0	35.0	37.0
6	35.72496557733836	37.0	35.0	37.0	33.0	37.0
7	35.94161895435481	37.0	35.0	37.0	35.0	37.0
8	35.87836585948786	37.0	35.0	37.0	35.0	37.0
9	37.69341834479345	39.0	38.0	39.0	35.0	39.0
10	37.0024365533768	39.0	37.0	39.0	33.0	39.0
11	36.97273154131037	39.0	37.0	39.0	33.0	39.0
12	36.97639060150296	39.0	37.0	39.0	33.0	39.0
13	36.98269772144188	39.0	37.0	39.0	33.0	39.0
14	38.166179496995554	40.0	37.0	41.0	33.0	41.0
15	38.258077957017676	40.0	38.0	41.0	33.0	41.0
16	38.302318744328986	40.0	38.0	41.0	34.0	41.0
17	38.254193642541544	40.0	37.0	41.0	34.0	41.0
18	38.17793290594946	40.0	37.0	41.0	34.0	41.0
19	38.08412560094247	40.0	37.0	41.0	34.0	41.0
20	38.01640337648768	40.0	37.0	41.0	34.0	41.0
21	37.93506860209095	40.0	36.0	41.0	33.0	41.0
22	37.90886719303552	40.0	36.0	41.0	33.0	41.0
23	37.81747423323653	40.0	36.0	41.0	33.0	41.0
24	37.78296802658212	40.0	36.0	41.0	33.0	41.0
25	37.76110144056988	40.0	36.0	41.0	33.0	41.0
26	37.68518757442381	40.0	36.0	41.0	33.0	41.0
27	37.65522346705471	40.0	36.0	41.0	33.0	41.0
28	37.54158320977836	40.0	36.0	41.0	33.0	41.0
29	37.4922683846624	40.0	36.0	41.0	33.0	41.0
30	37.36868627048703	40.0	36.0	41.0	32.0	41.0
31	37.235732309078486	39.0	36.0	41.0	32.0	41.0
32	37.08052258993779	39.0	35.0	41.0	31.0	41.0
33	36.95692461278471	39.0	35.0	41.0	31.0	41.0
34	36.84216231421809	39.0	35.0	41.0	31.0	41.0
35	36.708219560532065	39.0	35.0	41.0	30.0	41.0
36	36.60503723569635	39.0	35.0	41.0	30.0	41.0
37	36.56099843696793	39.0	35.0	41.0	30.0	41.0
38	36.490884075473936	39.0	35.0	41.0	30.0	41.0
39	36.421214934887765	39.0	35.0	41.0	30.0	41.0
40	36.335057815266104	39.0	35.0	41.0	30.0	41.0
41	36.20520982489388	39.0	35.0	41.0	29.0	41.0
42	36.12225808430221	39.0	35.0	41.0	29.0	41.0
43	36.05301618658246	39.0	35.0	41.0	29.0	41.0
44	35.9512488393588	39.0	35.0	41.0	29.0	41.0
45	35.879927834026724	39.0	35.0	40.0	28.0	41.0
46	35.740516583156904	38.0	35.0	40.0	28.0	41.0
47	35.64540005372262	38.0	35.0	40.0	27.0	41.0
48	35.524804409484794	38.0	35.0	40.0	27.0	41.0
49	35.48734557391196	38.0	35.0	40.0	27.0	41.0
50	35.40062690487924	38.0	34.0	40.0	27.0	41.0
51	34.059515777317635	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	1.0
10	4.0
11	9.0
12	8.0
13	18.0
14	32.0
15	43.0
16	88.0
17	168.0
18	312.0
19	631.0
20	1071.0
21	1728.0
22	2629.0
23	3899.0
24	5597.0
25	7865.0
26	9787.0
27	10941.0
28	11529.0
29	13087.0
30	16071.0
31	20068.0
32	25637.0
33	33949.0
34	54084.0
35	69630.0
36	74797.0
37	109418.0
38	193101.0
39	279309.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.92363774035055	19.368590035088907	23.224668410889194	11.483103813671349
2	34.25863844889689	24.25258936672878	23.838565648404504	17.650206535969833
3	32.25884572513901	24.379704694806883	27.020678977747416	16.34077060230669
4	26.285800096869917	27.9473941357744	26.80124111937631	18.965564647979374
5	23.716209213640468	32.68503105971036	26.163337908921125	17.43542181772804
6	22.889007802469973	38.11207299507824	25.539499872038647	13.459419330413136
7	82.2689980308757	6.40388410296976	8.139293864834741	3.1878240013198003
8	84.14368473706585	5.01439300844545	7.422921791289745	3.419000463198949
9	78.52438351180946	7.756467336013824	9.413513987973749	4.30563516420297
10	34.28401921323861	30.655733197405237	20.226354116654225	14.83389347270193
11	27.874953204215746	29.03548865374081	24.871351250742915	18.218206891300532
12	23.29372524053562	26.5218412052479	31.39103509102177	18.79339846319472
13	23.07322985031694	27.799445430299137	31.636911245582162	17.490413473801762
14	21.912694400791878	29.511589491517537	30.253553835773765	18.32216227191682
15	21.939238450165927	28.481341965613293	31.917474444742904	17.661945139477876
16	23.31561614978035	27.723620396828252	31.090907552680946	17.869855900710448
17	23.153179257993354	27.238107525608136	29.769310002770734	19.83940321362778
18	24.55007307545067	26.317631805481824	30.454379133627608	18.677915985439903
19	23.322913119528593	28.578000376481338	28.15435311834416	19.944733385645907
20	23.54488905433387	28.192318511671978	29.138703762063795	19.124088671930355
21	23.450239953976215	28.379607401876903	29.699724407200524	18.470428236946358
22	23.158043904492185	26.175711031537713	28.98409260594883	21.68215245802127
23	20.813284292056455	29.518463448526756	29.19760828597353	20.470643973443263
24	20.89862711215548	28.043206521164382	30.906050985725436	20.152115380954697
25	21.168297733286238	29.40234645166339	28.922544252420163	20.506811562630208
26	20.997083327164397	29.38859853764496	30.057910443973018	19.55640769121762
27	22.1538116620382	28.42984016463656	29.246572010516097	20.169776162809143
28	20.024365533768048	29.42148777810444	30.220770348499045	20.33337633962847
29	21.15116571735558	27.84301574241908	29.855604601532576	21.150213938692765
30	21.977626856232778	27.875376216954773	29.88310042956944	20.263896497243014
31	22.0666710377983	28.733351804889608	28.027660803005084	21.17231635430701
32	21.58094666020867	28.934705868667237	28.16429391771133	21.32005355341276
33	21.718531553577737	28.490225233132893	27.036224695906718	22.75501851738265
34	20.208481828430262	28.949934327272263	29.11099642765742	21.730587416640052
35	21.48037538150461	27.969179291834372	28.545428395576135	22.00501693108488
36	22.27109194393389	29.19507020953936	26.946545995232647	21.5872918512941
37	20.872400322335707	29.334241400679783	27.654986579920855	22.138371697063658
38	20.928238003887486	29.051140125084867	27.2213985224165	22.799223348611143
39	21.478048811439958	28.358985530849264	27.617655705701576	22.545309952009205
40	22.556519789593462	28.39874872831795	28.20342259607148	20.841308886017103
41	20.860238706088634	28.449616010186148	28.39779694965514	22.29234833407008
42	21.789703446919304	28.372944951237205	28.343439812689958	21.49391178915353
43	22.206688254416783	27.418839718358118	28.60232360897548	21.772148418249614
44	21.288962117094158	28.151392029170957	27.315413103665616	23.24423275006927
45	21.673692203240698	27.65689013724648	27.22975302401232	23.4396646355005
46	21.85495316191447	28.2065951916142	27.647055091064065	22.291396555407267
47	21.207955177570174	28.81435874441359	28.759049828785592	21.218636249230645
48	21.40306980344713	28.030939151732554	28.319222333380566	22.246768711439746
49	22.1330840378258	27.166618372712293	28.598410741139467	22.101886848322437
50	20.559159389085004	27.53643725980808	28.233773760096785	23.670629591010133
51	20.289171508399974	26.867336859849534	27.62019378213575	25.223297849614738
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3587.0
1	3307.0
2	3027.0
3	4735.0
4	6443.0
5	4695.5
6	2948.0
7	3028.5
8	3109.0
9	3329.0
10	3549.0
11	3611.0
12	3673.0
13	3721.5
14	3770.0
15	3872.5
16	3975.0
17	3929.0
18	3883.0
19	3979.5
20	4076.0
21	4405.5
22	4735.0
23	5424.0
24	6113.0
25	7181.0
26	9440.5
27	10632.0
28	11847.5
29	13063.0
30	16777.0
31	20491.0
32	23285.5
33	26080.0
34	28896.0
35	31712.0
36	34072.0
37	36432.0
38	40005.5
39	43579.0
40	46401.0
41	49223.0
42	52411.0
43	55599.0
44	59460.5
45	63322.0
46	65919.0
47	68516.0
48	72164.0
49	75812.0
50	79819.0
51	83826.0
52	82086.0
53	80346.0
54	71428.0
55	62510.0
56	54750.0
57	46990.0
58	40064.5
59	33139.0
60	29133.5
61	25128.0
62	21491.5
63	17855.0
64	15243.0
65	12631.0
66	10184.5
67	7738.0
68	6611.0
69	5484.0
70	4779.5
71	4075.0
72	3668.5
73	3262.0
74	3101.5
75	2326.0
76	1711.0
77	1289.0
78	867.0
79	752.5
80	638.0
81	587.0
82	536.0
83	351.0
84	166.0
85	119.0
86	72.0
87	48.0
88	24.0
89	18.0
90	12.0
91	12.5
92	13.0
93	15.0
94	17.0
95	14.0
96	11.0
97	9.0
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	945598.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.50358658667474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.79690771425823	21.319801354488792
2	9.410669455596647	5.364756633323485
3	3.3720385842613747	2.8834558128030654
4	1.76446775488552	2.0117463772310002
5	1.1002373887624701	1.5680355838243991
6	0.832656756368951	1.4240222371285276
7	0.696935048027002	1.3905603940708178
8	0.5915385957050684	1.348877726563152
9	0.4973345171248029	1.2758235724268
>10	5.941039598032887	39.160970470519935
>50	0.9102007976477218	16.878614623749577
>100	0.0814882003207695	3.6959792440108488
>500	0.002616593588281577	0.5569167029445833
>1k	0.0018689954202011265	1.120439266915102
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3143	0.3323822596917506	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2984	0.3155675033153623	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCC	1818	0.192259289888515	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1340	0.141709267574593	No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	1231	0.13018217043606267	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	957	0.10120579781260113	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0575318475715896E-4	0.0	0.0	0.09359156851008568	0.0
2	1.0575318475715896E-4	0.0	0.0	0.4157157692803919	0.0
3	1.0575318475715896E-4	0.0	0.0	0.6007838426054201	0.0
4	2.115063695143179E-4	0.0	0.0	0.8447564398401858	0.0
5	2.115063695143179E-4	0.0	0.0	1.527499000632404	0.0
6	2.115063695143179E-4	0.0	0.0	2.040296193519868	0.0
7	2.115063695143179E-4	0.0	0.0	2.488901203259736	0.0
8	2.115063695143179E-4	0.0	0.0	3.2413351128069223	0.0
9	2.115063695143179E-4	0.0	0.0	3.577841746704202	0.0
10	2.115063695143179E-4	0.0	0.0	4.3571369651797065	0.0
11	2.115063695143179E-4	0.0	0.0	5.395104473571221	0.0
12	2.115063695143179E-4	0.0	0.0	6.073616906973154	0.0
13	3.172595542714769E-4	0.0	0.0	6.36147707588214	0.0
14	3.172595542714769E-4	0.0	0.0	6.469451077519199	0.0
15	3.172595542714769E-4	0.0	0.0	6.669959115818773	0.0
16	3.172595542714769E-4	0.0	0.0	7.132840805500858	0.0
17	3.172595542714769E-4	0.0	0.0	7.721357278674447	0.0
18	3.172595542714769E-4	0.0	0.0	8.328169052811026	0.0
19	5.287659237857948E-4	0.0	0.0	8.674616486075479	1.0575318475715896E-4
20	5.287659237857948E-4	0.0	0.0	9.007633264875771	1.0575318475715896E-4
21	5.287659237857948E-4	0.0	0.0	9.424935331927522	1.0575318475715896E-4
22	5.287659237857948E-4	0.0	0.0	9.894162212695035	1.0575318475715896E-4
23	5.287659237857948E-4	0.0	0.0	10.39278847882504	1.0575318475715896E-4
24	6.345191085429538E-4	0.0	0.0	10.77286542484227	1.0575318475715896E-4
25	6.345191085429538E-4	0.0	0.0	11.12534078963788	1.0575318475715896E-4
26	6.345191085429538E-4	0.0	0.0	11.472422742010876	1.0575318475715896E-4
27	6.345191085429538E-4	0.0	0.0	11.805968286734956	1.0575318475715896E-4
28	6.345191085429538E-4	0.0	0.0	12.158972417454352	1.0575318475715896E-4
29	6.345191085429538E-4	0.0	0.0	12.503093280654147	1.0575318475715896E-4
30	6.345191085429538E-4	0.0	0.0	12.927586564269383	1.0575318475715896E-4
31	7.402722933001128E-4	0.0	0.0	13.32035389245747	1.0575318475715896E-4
32	7.402722933001128E-4	0.0	0.0	13.70307466809363	1.0575318475715896E-4
33	7.402722933001128E-4	0.0	0.0	14.080084771752901	1.0575318475715896E-4
34	7.402722933001128E-4	0.0	0.0	14.463968832421388	1.0575318475715896E-4
35	7.402722933001128E-4	0.0	0.0	14.844468791177647	1.0575318475715896E-4
36	8.460254780572716E-4	0.0	0.0	15.224862996749147	1.0575318475715896E-4
37	8.460254780572716E-4	0.0	0.0	15.593730105182118	1.0575318475715896E-4
38	8.460254780572716E-4	0.0	0.0	15.964818030494989	1.0575318475715896E-4
39	8.460254780572716E-4	0.0	0.0	16.336328968546887	1.0575318475715896E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGTCG	20	7.032651E-4	45.000004	1
GTGGCGT	20	7.032651E-4	45.000004	13
CGATCTA	20	7.032651E-4	45.000004	10
GACGTTG	30	2.1650922E-6	44.999996	1
ACGGGTC	170	0.0	41.02941	5
TCGATAG	55	6.002665E-11	40.909092	1
TCGTAAG	40	3.4573532E-7	39.375004	1
TCATCGA	40	3.4573532E-7	39.375004	16
GCTACGA	40	3.4573532E-7	39.375004	10
GCTAGCG	115	0.0	39.130432	1
GTACGAG	75	0.0	39.0	1
CTACGAA	35	6.2471154E-6	38.571426	11
TCACCGC	100	0.0	38.25	24
ACCGCTA	100	0.0	38.25	26
TACGGGT	200	0.0	38.25	4
CGCTACA	100	0.0	38.25	28
GCGTAAG	95	0.0	37.894737	1
CGGGATA	125	0.0	37.800003	6
AACGGGA	365	0.0	37.602737	4
ACGGGAA	420	0.0	37.5	5
>>END_MODULE