FastQCFastQC Report
Sat 18 Jun 2016
SRR3553674_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553674_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523137
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28700.5486134607187028No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15380.29399564550012713No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC10680.20415302301309218No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10440.1995653146307755No Hit
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC8980.1716567553050157TruSeq Adapter, Index 18 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCC8610.16458403821561082No Hit
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC6640.12692659857742808RNA PCR Primer, Index 34 (96% over 25bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT6320.12080965406767252No Hit
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT6320.12080965406767252RNA PCR Primer, Index 34 (96% over 26bp)
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT5350.10226766602247596No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG253.8878803E-545.0000041
CGGCCTA253.8878803E-545.00000444
CGGTCTA253.8878803E-545.00000431
TACCGTT253.8878803E-545.00000414
ACCGGGT351.2102282E-745.04
GCGCGTA207.0297596E-445.06
CGTTGTA351.2102282E-745.020
TACGATT351.2102282E-745.011
AACGCCC207.0297596E-445.027
CGGGATA406.8030204E-945.06
TTAACGG406.8030204E-945.02
ATTGGTA207.0297596E-445.04
ATACGTA207.0297596E-445.041
AATAGCG453.8380676E-1045.01
CGCGTAT207.0297596E-445.07
TTCGCCC207.0297596E-445.036
TATCGTG207.0297596E-445.01
ACGGTTT207.0297596E-445.029
ACGCATT1300.043.2692317
TACGCAT1300.043.2692316