Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553674_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 523137 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 48 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2870 | 0.5486134607187028 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1538 | 0.29399564550012713 | No Hit |
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC | 1068 | 0.20415302301309218 | No Hit |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1044 | 0.1995653146307755 | No Hit |
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC | 898 | 0.1716567553050157 | TruSeq Adapter, Index 18 (95% over 23bp) |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCC | 861 | 0.16458403821561082 | No Hit |
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC | 664 | 0.12692659857742808 | RNA PCR Primer, Index 34 (96% over 25bp) |
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 632 | 0.12080965406767252 | No Hit |
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT | 632 | 0.12080965406767252 | RNA PCR Primer, Index 34 (96% over 26bp) |
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 535 | 0.10226766602247596 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTCGACG | 25 | 3.8878803E-5 | 45.000004 | 1 |
CGGCCTA | 25 | 3.8878803E-5 | 45.000004 | 44 |
CGGTCTA | 25 | 3.8878803E-5 | 45.000004 | 31 |
TACCGTT | 25 | 3.8878803E-5 | 45.000004 | 14 |
ACCGGGT | 35 | 1.2102282E-7 | 45.0 | 4 |
GCGCGTA | 20 | 7.0297596E-4 | 45.0 | 6 |
CGTTGTA | 35 | 1.2102282E-7 | 45.0 | 20 |
TACGATT | 35 | 1.2102282E-7 | 45.0 | 11 |
AACGCCC | 20 | 7.0297596E-4 | 45.0 | 27 |
CGGGATA | 40 | 6.8030204E-9 | 45.0 | 6 |
TTAACGG | 40 | 6.8030204E-9 | 45.0 | 2 |
ATTGGTA | 20 | 7.0297596E-4 | 45.0 | 4 |
ATACGTA | 20 | 7.0297596E-4 | 45.0 | 41 |
AATAGCG | 45 | 3.8380676E-10 | 45.0 | 1 |
CGCGTAT | 20 | 7.0297596E-4 | 45.0 | 7 |
TTCGCCC | 20 | 7.0297596E-4 | 45.0 | 36 |
TATCGTG | 20 | 7.0297596E-4 | 45.0 | 1 |
ACGGTTT | 20 | 7.0297596E-4 | 45.0 | 29 |
ACGCATT | 130 | 0.0 | 43.26923 | 17 |
TACGCAT | 130 | 0.0 | 43.26923 | 16 |