##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553674_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	523137
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04465751801153	33.0	31.0	34.0	30.0	34.0
2	32.194471046781246	34.0	31.0	34.0	30.0	34.0
3	32.21671378625484	34.0	31.0	34.0	30.0	34.0
4	35.78085472830253	37.0	35.0	37.0	35.0	37.0
5	35.70215450254904	37.0	35.0	37.0	33.0	37.0
6	35.662468913496845	37.0	35.0	37.0	33.0	37.0
7	35.820182858409936	37.0	35.0	37.0	35.0	37.0
8	35.712673735560664	37.0	35.0	37.0	35.0	37.0
9	37.52915966563252	39.0	37.0	39.0	35.0	39.0
10	36.96698379200859	39.0	37.0	39.0	33.0	39.0
11	36.89870722200877	39.0	37.0	39.0	33.0	39.0
12	36.72545814958605	39.0	35.0	39.0	33.0	39.0
13	36.62054872815343	39.0	35.0	39.0	32.0	39.0
14	37.72740410255822	40.0	36.0	41.0	33.0	41.0
15	37.86843790441127	40.0	36.0	41.0	33.0	41.0
16	37.97030796904061	40.0	36.0	41.0	33.0	41.0
17	37.9124760817912	40.0	36.0	41.0	33.0	41.0
18	37.87832823906548	40.0	36.0	41.0	33.0	41.0
19	37.756226380470125	39.0	36.0	41.0	33.0	41.0
20	37.66352217487962	39.0	35.0	41.0	33.0	41.0
21	37.568948478123325	39.0	35.0	41.0	33.0	41.0
22	37.53715948212419	39.0	35.0	41.0	33.0	41.0
23	37.43967067899995	39.0	35.0	41.0	33.0	41.0
24	37.41458738341964	39.0	35.0	41.0	33.0	41.0
25	37.32007485610844	39.0	35.0	41.0	33.0	41.0
26	37.266477041386864	39.0	35.0	41.0	32.0	41.0
27	37.21817420675655	39.0	35.0	41.0	32.0	41.0
28	37.15356206882709	39.0	35.0	41.0	32.0	41.0
29	37.07032956950091	39.0	35.0	41.0	32.0	41.0
30	36.8960520857825	39.0	35.0	41.0	31.0	41.0
31	36.77356982970044	39.0	35.0	41.0	31.0	41.0
32	36.63671466556562	39.0	35.0	41.0	31.0	41.0
33	36.520951490718495	39.0	35.0	41.0	31.0	41.0
34	36.41903554900533	39.0	35.0	41.0	30.0	41.0
35	36.23611596962172	39.0	35.0	41.0	30.0	41.0
36	36.09123996199848	38.0	35.0	40.0	30.0	41.0
37	36.023921076123465	38.0	35.0	40.0	30.0	41.0
38	35.91107874227975	38.0	35.0	40.0	29.0	41.0
39	35.88084765558544	38.0	35.0	40.0	29.0	41.0
40	35.6987423944397	38.0	35.0	40.0	28.0	41.0
41	35.59141869147088	38.0	35.0	40.0	28.0	41.0
42	35.49469450641037	38.0	34.0	40.0	28.0	41.0
43	35.406908706514734	38.0	34.0	40.0	27.0	41.0
44	35.29234407048249	38.0	34.0	40.0	27.0	41.0
45	35.21087592733834	38.0	34.0	40.0	27.0	41.0
46	35.04370748006736	37.0	34.0	40.0	26.0	41.0
47	34.9159608286166	37.0	34.0	40.0	26.0	41.0
48	34.77705457652584	37.0	34.0	40.0	25.0	41.0
49	34.74045422136075	37.0	34.0	40.0	26.0	41.0
50	34.62709959341434	37.0	34.0	40.0	25.0	41.0
51	33.24198823635109	35.0	31.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	1.0
10	1.0
11	8.0
12	7.0
13	7.0
14	21.0
15	38.0
16	82.0
17	125.0
18	283.0
19	456.0
20	721.0
21	1159.0
22	1652.0
23	2406.0
24	3467.0
25	4296.0
26	5127.0
27	6285.0
28	7184.0
29	8615.0
30	10418.0
31	12975.0
32	16865.0
33	22897.0
34	39150.0
35	49908.0
36	44257.0
37	61824.0
38	97422.0
39	125426.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.675748417718495	21.109193194134615	23.91629726056463	11.298761127582258
2	32.324993261803314	25.506511678585152	24.417504401332728	17.750990658278806
3	30.854059261723027	25.463693067016862	26.853768706858816	16.828478964401295
4	27.954818718614817	28.168911776456262	24.997276048148	18.878993456780922
5	24.050296576231464	32.23801795705523	24.35901111945819	19.352674347255117
6	24.599865809529817	37.104238469081714	23.912283015730104	14.383612705658363
7	81.26819552048508	7.484081607685941	6.9866975572364405	4.261025314592545
8	82.46807241697681	6.370606552394498	6.841611279645676	4.319709750983012
9	77.20577974794367	8.691031221267087	8.78163846181784	5.321550568971416
10	40.30837046509805	27.087741834356965	16.75335523964086	15.850532460904123
11	31.49366227202435	27.61150520800479	21.274733004929875	19.62009951504099
12	27.614945989291524	24.195574008338163	28.663046200134957	19.52643380223536
13	24.47007189321344	26.894484618751875	29.097731569359457	19.537711918675225
14	20.509923786694497	30.357439829337245	28.737022997799812	20.39561338616844
15	20.209237733136824	27.575759313525904	31.866987041635365	20.348015911701907
16	21.880692820427537	24.483261554812604	32.0854766533432	21.550568971416663
17	21.615752661348747	24.384625824592792	28.07964261751702	25.91997889654144
18	23.05094076695015	26.465916194037124	28.47074475710951	22.012398281903213
19	23.524048193876556	27.764237666232745	26.883588811343873	21.828125328546825
20	25.074311318067732	26.653438774164318	25.580297321734076	22.69195258603387
21	23.95758663600548	28.14138552616236	26.646366057074918	21.25466178075724
22	22.398339249565602	26.19734409915567	24.55437103473851	26.849945616540218
23	21.235546329164254	28.586966702794868	26.171920548537	24.005566419503875
24	21.886236301389502	27.976610333430823	28.26735635216014	21.869797013019536
25	22.057510747662658	27.200714153271516	26.91015928905812	23.831615810007705
26	20.79627325155743	28.99068504043874	26.414113320220135	23.798928387783697
27	22.077008508287506	27.471962411375987	28.179807583864264	22.271221496472243
28	21.162142995047187	27.650500729254478	28.821130984808953	22.366225290889385
29	22.961289298979043	26.907674280351035	27.019881981201866	23.111154439468056
30	22.809321458814804	26.85013677105615	27.808967822960334	22.531573947168717
31	24.95656013625494	28.508211042231768	24.679959551704428	21.855269269808865
32	25.271009314959564	28.10353693200825	25.081957498704927	21.54349625432726
33	24.247568036671083	27.144897034619998	24.08393977103512	24.523595157673803
34	22.78810330754659	27.857712224522444	24.886406428908682	24.467778039022285
35	22.911971433869137	27.082580662426857	25.69193155903712	24.313516344666887
36	25.192253654396456	26.549833026530333	26.036774305774586	22.221139013298618
37	23.494419243907426	28.12074083844194	25.947505146835343	22.437334770815294
38	23.356979146953858	28.44704159713421	24.598336573402378	23.597642682509555
39	23.471098392963984	26.608708617436733	25.35855808325544	24.561634906343848
40	22.903751789684154	27.27201478771335	27.462404685579493	22.361828737023
41	20.862221559553234	27.682614687930695	26.219709177519462	25.23545457499661
42	22.526221620722676	27.097873023701247	25.53652293758614	24.839382417989935
43	22.71928768181184	25.236028038544394	26.75972068502132	25.28496359462244
44	22.989197858304802	25.354543838420913	26.342621531262367	25.31363677201192
45	23.936750793769125	27.281954822541703	25.504217824393994	23.277076559295175
46	23.575659913177617	26.99789921186993	26.2692181971453	23.15722267780715
47	22.29377772935197	27.64228108506949	26.889896910369558	23.17404427520898
48	22.46543448465698	26.42118603730954	27.33624270506579	23.777136772967694
49	23.0461619040519	25.341545331337677	27.741107969805235	23.871184794805185
50	21.951802300353446	25.665552235838796	27.95539218216261	24.427253281645154
51	21.791041352456432	25.23583688402847	26.17306747563258	26.800054287882524
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3270.0
1	3160.5
2	3051.0
3	2959.0
4	2867.0
5	2160.0
6	1453.0
7	1504.0
8	1555.0
9	1665.0
10	1775.0
11	1802.5
12	1830.0
13	1963.0
14	2096.0
15	2045.5
16	1995.0
17	1930.5
18	1866.0
19	1901.0
20	1936.0
21	2118.0
22	2300.0
23	2356.0
24	2412.0
25	2813.0
26	3677.0
27	4140.0
28	4591.0
29	5042.0
30	6453.0
31	7864.0
32	9214.5
33	10565.0
34	11261.5
35	11958.0
36	13293.0
37	14628.0
38	15703.5
39	16779.0
40	18345.0
41	19911.0
42	22553.0
43	25195.0
44	27759.0
45	30323.0
46	31883.5
47	33444.0
48	36860.0
49	40276.0
50	43271.0
51	46266.0
52	45736.5
53	45207.0
54	41063.0
55	36919.0
56	33062.0
57	29205.0
58	26506.5
59	23808.0
60	22483.5
61	21159.0
62	18953.0
63	16747.0
64	14429.0
65	12111.0
66	10728.5
67	9346.0
68	8977.5
69	8609.0
70	7802.0
71	6995.0
72	6246.0
73	5497.0
74	4513.5
75	2808.5
76	2087.0
77	1824.5
78	1562.0
79	1381.5
80	1201.0
81	888.5
82	576.0
83	455.5
84	335.0
85	226.5
86	118.0
87	94.0
88	70.0
89	50.0
90	30.0
91	18.5
92	7.0
93	6.0
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	523137.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.865106862424778
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.11568995068342	21.20971834382985
2	9.562227018686215	5.329049554369138
3	3.380476389697347	2.8259200753446145
4	1.682107106939358	1.8748837715563758
5	1.0071707357108826	1.4032460089645362
6	0.6947135778477027	1.1614960851302214
7	0.5686477323923949	1.1091800881132727
8	0.44849419054607315	0.9997870837394424
9	0.34245315174741026	0.8588244301934179
>10	4.768668420189785	32.606854587878416
>50	1.3152235935522631	24.257206380496108
>100	0.10725294333201936	4.305904141654666
>500	0.004125113205077668	0.8088417550305215
>1k	0.0027500754700517786	1.2490876936994315
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2870	0.5486134607187028	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1538	0.29399564550012713	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1068	0.20415302301309218	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1044	0.1995653146307755	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	898	0.1716567553050157	TruSeq Adapter, Index 18 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCC	861	0.16458403821561082	No Hit
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	664	0.12692659857742808	RNA PCR Primer, Index 34 (96% over 25bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	632	0.12080965406767252	No Hit
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	632	0.12080965406767252	RNA PCR Primer, Index 34 (96% over 26bp)
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	535	0.10226766602247596	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.823090318597232E-4	0.0	0.0	0.1273089076092878	0.0
2	3.823090318597232E-4	0.0	0.0	0.5889470635799036	0.0
3	3.823090318597232E-4	0.0	0.0	0.8028489669054186	0.0
4	3.823090318597232E-4	0.0	0.0	1.0949330672462472	0.0
5	3.823090318597232E-4	0.0	0.0	1.9675534324660653	0.0
6	3.823090318597232E-4	0.0	0.0	2.5694990031291995	0.0
7	3.823090318597232E-4	0.0	0.0	3.1137159099815155	0.0
8	3.823090318597232E-4	0.0	0.0	3.954222316525117	0.0
9	3.823090318597232E-4	0.0	0.0	4.328120549683926	0.0
10	3.823090318597232E-4	0.0	0.0	5.222532529719748	0.0
11	3.823090318597232E-4	0.0	0.0	6.4973419964559955	0.0
12	3.823090318597232E-4	0.0	0.0	7.320262187534049	0.0
13	3.823090318597232E-4	0.0	0.0	7.660134916857343	0.0
14	3.823090318597232E-4	0.0	0.0	7.796619241231264	0.0
15	3.823090318597232E-4	0.0	0.0	8.029254287117906	0.0
16	3.823090318597232E-4	0.0	0.0	8.573280039454293	0.0
17	3.823090318597232E-4	0.0	0.0	9.240600454565438	0.0
18	3.823090318597232E-4	0.0	0.0	10.047845975337244	0.0
19	3.823090318597232E-4	0.0	0.0	10.406643001737594	0.0
20	3.823090318597232E-4	0.0	0.0	10.795642441654863	0.0
21	3.823090318597232E-4	0.0	0.0	11.25556020698211	0.0
22	3.823090318597232E-4	0.0	0.0	11.729049942940376	0.0
23	3.823090318597232E-4	0.0	0.0	12.227772075001386	0.0
24	3.823090318597232E-4	0.0	0.0	12.63512234844792	0.0
25	3.823090318597232E-4	0.0	0.0	12.968686978745529	0.0
26	3.823090318597232E-4	0.0	0.0	13.309324326132543	0.0
27	3.823090318597232E-4	0.0	0.0	13.638492402563765	0.0
28	3.823090318597232E-4	0.0	0.0	13.986775930587973	0.0
29	3.823090318597232E-4	0.0	0.0	14.314605925407685	0.0
30	3.823090318597232E-4	0.0	0.0	14.700929202101936	0.0
31	3.823090318597232E-4	0.0	0.0	15.072915890101445	0.0
32	3.823090318597232E-4	0.0	0.0	15.431139452954007	0.0
33	3.823090318597232E-4	0.0	0.0	15.797964969023411	0.0
34	3.823090318597232E-4	0.0	0.0	16.13114729028916	0.0
35	3.823090318597232E-4	0.0	0.0	16.550922607271136	0.0
36	3.823090318597232E-4	0.0	0.0	16.897103435620114	0.0
37	3.823090318597232E-4	0.0	0.0	17.2465338907399	0.0
38	3.823090318597232E-4	0.0	0.0	17.614697488420816	0.0
39	3.823090318597232E-4	0.0	0.0	17.979611459330922	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	25	3.8878803E-5	45.000004	1
CGGCCTA	25	3.8878803E-5	45.000004	44
CGGTCTA	25	3.8878803E-5	45.000004	31
TACCGTT	25	3.8878803E-5	45.000004	14
ACCGGGT	35	1.2102282E-7	45.0	4
GCGCGTA	20	7.0297596E-4	45.0	6
CGTTGTA	35	1.2102282E-7	45.0	20
TACGATT	35	1.2102282E-7	45.0	11
AACGCCC	20	7.0297596E-4	45.0	27
CGGGATA	40	6.8030204E-9	45.0	6
TTAACGG	40	6.8030204E-9	45.0	2
ATTGGTA	20	7.0297596E-4	45.0	4
ATACGTA	20	7.0297596E-4	45.0	41
AATAGCG	45	3.8380676E-10	45.0	1
CGCGTAT	20	7.0297596E-4	45.0	7
TTCGCCC	20	7.0297596E-4	45.0	36
TATCGTG	20	7.0297596E-4	45.0	1
ACGGTTT	20	7.0297596E-4	45.0	29
ACGCATT	130	0.0	43.26923	17
TACGCAT	130	0.0	43.26923	16
>>END_MODULE