FastQCFastQC Report
Sat 18 Jun 2016
SRR3553672_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553672_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1712224
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161830.945145027753378No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26990.1576312445100641No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACTA207.034257E-445.035
CGTTTTT121350.042.571081
TATACGG1350.041.6666682
TACGGGT2000.041.6254
TAGCACG1250.041.3999981
ACACGAC7050.039.25531826
AACACGT7000.038.8928641
CAACGTC356.2500803E-638.5714320
TCTAGCG1050.038.571431
TATTACG356.2500803E-638.571431
ATATGCG1000.038.251
TCGCTAA659.094947E-1238.0769215
GTACGAG1300.038.076921
TAACGCG601.5643309E-1037.5000041
CGTTCGA301.1401551E-437.50000414
TATGCGG3450.037.1739122
AAGCGAT850.037.05882313
ATAGCGG1950.036.923082
ACGACCA7400.036.7905428
CACGACC7600.036.71052627
Produced by FastQC (version 0.11.5)