##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553672_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1712224
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10812370344067	33.0	31.0	34.0	30.0	34.0
2	32.284084325415364	34.0	31.0	34.0	30.0	34.0
3	32.25868227521633	34.0	31.0	34.0	30.0	34.0
4	35.8302091315155	37.0	35.0	37.0	35.0	37.0
5	35.77036415796064	37.0	35.0	37.0	35.0	37.0
6	35.75133393761564	37.0	35.0	37.0	35.0	37.0
7	35.95319128805577	37.0	35.0	37.0	35.0	37.0
8	35.90614545760368	37.0	35.0	37.0	35.0	37.0
9	37.700677014222435	39.0	38.0	39.0	35.0	39.0
10	37.11673939858336	39.0	37.0	39.0	33.0	39.0
11	37.0096365896051	39.0	37.0	39.0	33.0	39.0
12	36.94220908012036	39.0	37.0	39.0	33.0	39.0
13	36.930039527538455	39.0	37.0	39.0	33.0	39.0
14	38.07140654493804	40.0	37.0	41.0	33.0	41.0
15	38.17290669912348	40.0	37.0	41.0	33.0	41.0
16	38.241568276113405	40.0	37.0	41.0	33.0	41.0
17	38.185789943371894	40.0	37.0	41.0	33.0	41.0
18	38.055130053077164	40.0	37.0	41.0	33.0	41.0
19	37.901276935728035	40.0	37.0	41.0	33.0	41.0
20	37.757201744631544	40.0	35.0	41.0	33.0	41.0
21	37.65405636178444	39.0	35.0	41.0	33.0	41.0
22	37.62967754219074	39.0	35.0	41.0	33.0	41.0
23	37.55588287513783	39.0	35.0	41.0	33.0	41.0
24	37.50771686414862	39.0	35.0	41.0	33.0	41.0
25	37.417072182144395	39.0	35.0	41.0	33.0	41.0
26	37.375146592969145	39.0	35.0	41.0	33.0	41.0
27	37.304839203281816	39.0	35.0	41.0	33.0	41.0
28	37.17576905825406	39.0	35.0	41.0	32.0	41.0
29	37.055527781411776	39.0	35.0	41.0	32.0	41.0
30	36.86703141645018	39.0	35.0	41.0	31.0	41.0
31	36.646140925486385	39.0	35.0	41.0	31.0	41.0
32	36.38172633954436	39.0	35.0	41.0	30.0	41.0
33	36.101882697590966	39.0	35.0	41.0	29.0	41.0
34	35.85978002878128	39.0	35.0	41.0	27.0	41.0
35	35.65187849253369	39.0	35.0	41.0	26.0	41.0
36	35.51827097389127	39.0	35.0	41.0	25.0	41.0
37	35.4250822322313	39.0	35.0	41.0	25.0	41.0
38	35.31871706038462	39.0	35.0	41.0	24.0	41.0
39	35.24620318369559	38.0	35.0	41.0	24.0	41.0
40	35.12933296110789	38.0	34.0	40.0	23.0	41.0
41	34.99592401461491	38.0	34.0	40.0	23.0	41.0
42	34.889676233950695	38.0	34.0	40.0	23.0	41.0
43	34.79283493281253	38.0	34.0	40.0	23.0	41.0
44	34.66350255574037	38.0	34.0	40.0	22.0	41.0
45	34.595867129534454	38.0	34.0	40.0	23.0	41.0
46	34.481778669146095	38.0	34.0	40.0	22.0	41.0
47	34.368729208327885	38.0	33.0	40.0	22.0	41.0
48	34.20285897172332	37.0	33.0	40.0	21.0	41.0
49	34.15344137215691	37.0	33.0	40.0	20.0	41.0
50	34.05253751845553	37.0	33.0	40.0	20.0	41.0
51	32.7341866484759	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	5.0
11	17.0
12	20.0
13	26.0
14	50.0
15	93.0
16	214.0
17	426.0
18	907.0
19	1622.0
20	2737.0
21	4410.0
22	6536.0
23	9947.0
24	15312.0
25	22964.0
26	29809.0
27	31356.0
28	30615.0
29	31484.0
30	34454.0
31	40107.0
32	49532.0
33	66437.0
34	105420.0
35	127083.0
36	136307.0
37	191348.0
38	318862.0
39	453980.0
40	138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.59712747864766	18.657371932644327	23.945640290055508	15.799860298652513
2	37.61744958603547	22.500502270730934	23.532902237090475	16.34914590614312
3	31.186281701459624	22.936017717308015	30.393745210906985	15.483955370325377
4	26.436727904760126	25.514769095632346	29.934809931410843	18.113693068196685
5	23.576296092100097	29.803109873474497	28.58930840824565	18.031285626179752
6	23.591539424748163	34.97632319135814	27.694565664305603	13.73757171958809
7	79.72549152447344	5.325062608630646	11.699170202029642	3.250275664866279
8	81.47678107537331	4.238172108322276	11.156250584035734	3.128796232268675
9	76.29001812846917	6.6115181191246	12.844989907862523	4.253473844543705
10	36.748462817949054	28.173825387332496	20.894871231801446	14.182840562917
11	28.167050572822248	26.756020240342387	27.964273366101633	17.112655820733735
12	24.809370736539144	24.206412245126806	32.469817033285366	18.514399985048684
13	23.941376829199918	24.261837236249463	33.433125572355024	18.3636603621956
14	20.930672622273722	26.62694834320743	32.946915824097786	19.495463210421065
15	20.824319715177452	26.26805838488422	34.457582652737024	18.4500392472013
16	23.879235427140376	24.741797802156725	33.16114013119778	18.217826639505112
17	24.43465340983423	23.98821649503803	31.477306707533593	20.099823387594146
18	24.338637935223428	24.122661520922495	31.873458145663186	19.66524239819089
19	23.75687994094231	26.17163408525987	30.54495206234698	19.526533911450837
20	24.159338964995232	25.800596183676898	30.495425832134117	19.54463901919375
21	23.85797652643579	25.858590932027585	31.512407255125495	18.771025286411124
22	22.835154746107985	23.737840376025567	31.602348758106412	21.82465611976003
23	21.51336507372867	26.40921982170557	31.55381538864074	20.52359971592502
24	20.96174332330349	25.89964864410264	32.591179658736245	20.547428373857628
25	22.375752238024933	26.412432018240604	30.88696338796793	20.324852355766534
26	21.514883566636144	26.500329396153777	30.708190049900015	21.27659698731007
27	21.637998299287943	25.739389238791187	31.297890930158673	21.3247215317622
28	20.587434821612124	26.31565729717607	32.062043284056294	21.03486459715551
29	21.801528304707794	25.932529855906704	30.927845889322892	21.33809595006261
30	22.09453903227615	25.14472405479657	31.134302521165452	21.626434391761826
31	22.33159913656157	26.320504793765302	30.004485394434372	21.343410675238754
32	22.24037275496664	26.55178294428766	29.459346440652624	21.74849786009307
33	22.060781766871624	26.528187900648515	28.272585829891415	23.138444502588445
34	21.104072831592127	27.07315164371017	29.46594604444278	22.35682948025492
35	22.078711663894442	26.732191582409776	28.367141215168107	22.821955538527668
36	22.133202197843275	28.180424991122656	27.58523417496776	22.10113863606631
37	21.95378642046835	28.044227858037267	27.839231315528814	22.162754405965572
38	21.993734464649485	28.723286205543204	27.688550096249088	21.594429233558227
39	22.763960790176988	26.65206187975405	27.479757321471958	23.104220008597007
40	23.09878847627413	25.79615751210122	29.539709757601806	21.56534425402284
41	21.093151363373018	26.678460294914686	28.570093632608817	23.65829470910348
42	21.735473863232848	27.22067907002822	28.371579886743792	22.67226717999514
43	21.528082774216458	26.18979759657615	29.27414870951464	23.00797091969275
44	21.906946754630237	26.8276814248603	28.621313566449253	22.64405825406022
45	22.388250602724877	26.26455417048237	27.832865326032106	23.514329900760647
46	21.83803053806044	26.97053656530921	27.7657596202366	23.425673276393745
47	21.195532827480516	27.17600033640458	28.812877287083932	22.815589549030967
48	20.901646046311697	27.825506475788213	28.51741360943428	22.755433868465808
49	21.73372175603192	26.978129029846563	28.9056805651597	22.38246864896182
50	20.58761003233222	27.29409236174706	28.95801016689405	23.160287439026668
51	21.06488403386473	26.482983534864594	27.949964490627394	24.50216794064328
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3137.0
1	3340.0
2	3543.0
3	15176.0
4	26809.0
5	18416.0
6	10023.0
7	10273.0
8	10523.0
9	10815.5
10	11108.0
11	11075.5
12	11043.0
13	10925.5
14	10808.0
15	10654.0
16	10500.0
17	10373.0
18	10246.0
19	10353.0
20	10460.0
21	10705.5
22	10951.0
23	11062.0
24	11173.0
25	13233.0
26	16482.5
27	17672.0
28	21172.5
29	24673.0
30	28347.0
31	32021.0
32	35224.0
33	38427.0
34	43839.5
35	49252.0
36	54460.5
37	59669.0
38	65441.5
39	71214.0
40	75717.5
41	80221.0
42	83744.0
43	87267.0
44	92543.5
45	97820.0
46	103501.0
47	109182.0
48	114195.0
49	119208.0
50	123482.0
51	127756.0
52	126114.5
53	124473.0
54	114753.5
55	105034.0
56	96051.5
57	87069.0
58	79105.5
59	71142.0
60	66708.0
61	62274.0
62	55062.0
63	47850.0
64	43063.0
65	38276.0
66	33300.0
67	28324.0
68	25171.0
69	22018.0
70	18880.0
71	15742.0
72	14469.0
73	13196.0
74	11815.0
75	9049.0
76	7664.0
77	5936.0
78	4208.0
79	3256.0
80	2304.0
81	1708.0
82	1112.0
83	811.0
84	510.0
85	407.5
86	305.0
87	205.0
88	105.0
89	93.0
90	81.0
91	57.5
92	34.0
93	41.0
94	48.0
95	29.5
96	11.0
97	10.0
98	9.0
99	5.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1712224.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.168558039150042
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.11900398693933	20.952121357406046
2	8.3939823714428	4.56104794476292
3	3.183001826366108	2.594327095750446
4	1.7504359860962697	1.9022728672829337
5	1.0678166124177313	1.450551880481986
6	0.7634541878834111	1.2445169648245569
7	0.5977016440455828	1.136708426444347
8	0.4774309474297054	1.0376888323944276
9	0.3832606725032131	0.93713758425245
>10	4.709229125634935	30.671830611786582
>50	1.397835899977966	25.93262823984586
>100	0.15273059649466564	5.6356863551125675
>500	0.0028163082099174497	0.5288365938612568
>1k	0.0010831954653528653	0.46215079966595307
>5k	0.0	0.0
>10k+	2.1663909307057306E-4	0.9524944461276257
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16183	0.945145027753378	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2699	0.1576312445100641	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.504214401853963E-4	0.0	0.0	0.037378286953108936	0.0
2	3.504214401853963E-4	0.0	0.0	0.17649559870671128	0.0
3	3.504214401853963E-4	0.0	0.0	0.2864695273515615	0.0
4	3.504214401853963E-4	0.0	0.0	0.44264068252751976	0.0
5	3.504214401853963E-4	0.0	0.0	0.8581821070140355	0.0
6	3.504214401853963E-4	0.0	0.0	1.2787462388098754	0.0
7	3.504214401853963E-4	0.0	0.0	1.6202903358439082	0.0
8	3.504214401853963E-4	0.0	0.0	2.1921781262264752	0.0
9	3.504214401853963E-4	0.0	0.0	2.5035275758311997	0.0
10	3.504214401853963E-4	0.0	0.0	3.030444614723307	0.0
11	3.504214401853963E-4	0.0	0.0	3.641521202833274	0.0
12	3.504214401853963E-4	0.0	0.0	4.085797185414992	0.0
13	3.504214401853963E-4	0.0	0.0	4.291260956510363	0.0
14	3.504214401853963E-4	0.0	0.0	4.394051245631413	0.0
15	3.504214401853963E-4	0.0	0.0	4.525751303567757	0.0
16	3.504214401853963E-4	0.0	0.0	4.809417459397836	0.0
17	3.504214401853963E-4	0.0	0.0	5.13414132730297	0.0
18	3.504214401853963E-4	0.0	0.0	5.523868372362495	0.0
19	3.504214401853963E-4	0.0	0.0	5.743874633225559	0.0
20	3.504214401853963E-4	0.0	0.0	5.969078812118041	0.0
21	3.504214401853963E-4	0.0	0.0	6.260395836058834	0.0
22	4.08825013549629E-4	0.0	0.0	6.572854953557479	0.0
23	4.6722858691386173E-4	0.0	0.0	6.901258246584559	0.0
24	6.424393070065599E-4	0.0	0.0	7.167228119685275	0.0
25	6.424393070065599E-4	0.0	0.0	7.401368045302484	0.0
26	6.424393070065599E-4	0.0	0.0	7.632821406544938	0.0
27	7.008428803707926E-4	0.0	0.0	7.880335750462557	0.0
28	7.592464537350253E-4	0.0	0.0	8.140523669800213	0.0
29	7.592464537350253E-4	0.0	0.0	8.42045199693498	0.0
30	8.17650027099258E-4	0.0	0.0	8.749088904255517	0.0
31	8.760536004634908E-4	0.0	0.0	9.03789457454165	0.0
32	8.760536004634908E-4	0.0	0.0	9.326233016240865	0.0
33	9.344571738277235E-4	0.0	0.0	9.615447511540546	0.0
34	9.928607471919562E-4	0.0	0.0	9.90472041041359	0.0
35	9.928607471919562E-4	0.0	0.0	10.214960192124394	0.0
36	9.928607471919562E-4	0.0	0.0	10.501079298035771	0.0
37	0.001051264320556189	0.0	0.0	10.804252247369503	0.0
38	0.001051264320556189	0.0	0.0	11.141474479974583	0.0
39	0.001051264320556189	0.0	0.0	11.57249285140262	5.8403573364232716E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGACTA	20	7.034257E-4	45.0	35
CGTTTTT	12135	0.0	42.57108	1
TATACGG	135	0.0	41.666668	2
TACGGGT	200	0.0	41.625	4
TAGCACG	125	0.0	41.399998	1
ACACGAC	705	0.0	39.255318	26
AACACGT	700	0.0	38.89286	41
CAACGTC	35	6.2500803E-6	38.57143	20
TCTAGCG	105	0.0	38.57143	1
TATTACG	35	6.2500803E-6	38.57143	1
ATATGCG	100	0.0	38.25	1
TCGCTAA	65	9.094947E-12	38.07692	15
GTACGAG	130	0.0	38.07692	1
TAACGCG	60	1.5643309E-10	37.500004	1
CGTTCGA	30	1.1401551E-4	37.500004	14
TATGCGG	345	0.0	37.173912	2
AAGCGAT	85	0.0	37.058823	13
ATAGCGG	195	0.0	36.92308	2
ACGACCA	740	0.0	36.79054	28
CACGACC	760	0.0	36.710526	27
>>END_MODULE