FastQCFastQC Report
Sat 18 Jun 2016
SRR3553671_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553671_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1403689
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186041.3253648065917734No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23380.1665611114712732No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCC21400.15245542281801738No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCT15440.1099958751546817No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT15200.1082860947118628TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG351.2121927E-745.021
CGTTCTA207.033821E-445.027
TCGTCGG207.033821E-445.02
CGTTTTT176750.043.5360681
TGCGACG659.094947E-1238.076921
GCGAGAC4350.037.7586221
ACACGAC4250.037.58823426
GACACGA4250.037.58823425
TAGCACG601.5643309E-1037.4999961
ATACGCG301.1400509E-437.4999961
TATCGCG601.5643309E-1037.4999961
GTTTTTT207700.037.4819452
TACGCAT850.037.05882316
CGAGACA4500.036.522
TACGAAT1050.036.4285712
CGACCAA4450.036.40449529
CACGACC4450.036.40449527
ACGACCA4550.036.098928
GCAACCG504.8803486E-836.0000041
CGCGTAA504.8803486E-836.00000431
Produced by FastQC (version 0.11.5)