##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553671_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1403689
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1363628268085	33.0	31.0	34.0	30.0	34.0
2	32.35627336254683	34.0	31.0	34.0	30.0	34.0
3	32.27509298712179	34.0	31.0	34.0	30.0	34.0
4	35.873801817924054	37.0	35.0	37.0	35.0	37.0
5	35.82763062188277	37.0	35.0	37.0	35.0	37.0
6	35.81000349792582	37.0	35.0	37.0	35.0	37.0
7	36.01096396708957	37.0	35.0	37.0	35.0	37.0
8	35.98877030453327	37.0	35.0	37.0	35.0	37.0
9	37.77422919179391	39.0	38.0	39.0	35.0	39.0
10	37.177049902079446	39.0	37.0	39.0	34.0	39.0
11	37.137901629207036	39.0	37.0	39.0	34.0	39.0
12	37.11361989728494	39.0	37.0	39.0	34.0	39.0
13	37.15057466433092	39.0	37.0	39.0	33.0	39.0
14	38.39011134232725	40.0	38.0	41.0	34.0	41.0
15	38.4726296209488	40.0	38.0	41.0	34.0	41.0
16	38.501019100384774	40.0	38.0	41.0	34.0	41.0
17	38.44968935426579	40.0	38.0	41.0	34.0	41.0
18	38.2398586866464	39.0	38.0	41.0	34.0	41.0
19	37.977609000284254	39.0	37.0	41.0	34.0	41.0
20	37.716593205475	39.0	35.0	41.0	34.0	41.0
21	37.64354568568964	39.0	35.0	41.0	33.0	41.0
22	37.59829207181933	39.0	35.0	41.0	33.0	41.0
23	37.543102496350684	39.0	35.0	41.0	33.0	41.0
24	37.493226063608105	39.0	35.0	41.0	33.0	41.0
25	37.422691921073685	39.0	35.0	41.0	33.0	41.0
26	37.35339024527513	39.0	35.0	41.0	33.0	41.0
27	37.2915567479691	39.0	35.0	41.0	33.0	41.0
28	37.176487099350354	39.0	35.0	41.0	33.0	41.0
29	37.02412642686521	39.0	35.0	41.0	32.0	41.0
30	36.7591589020075	39.0	35.0	41.0	31.0	41.0
31	36.432086452198455	39.0	35.0	41.0	30.0	41.0
32	35.97714094788803	39.0	35.0	41.0	28.0	41.0
33	35.537485867596025	39.0	35.0	41.0	24.0	41.0
34	35.18415119018529	39.0	35.0	41.0	22.0	41.0
35	34.90768325462407	39.0	35.0	41.0	20.0	41.0
36	34.75449476344119	39.0	35.0	41.0	18.0	41.0
37	34.667736229321456	39.0	34.0	41.0	18.0	41.0
38	34.56686345764624	39.0	34.0	41.0	18.0	41.0
39	34.48489872044306	38.0	34.0	41.0	18.0	41.0
40	34.357657572297	38.0	34.0	40.0	18.0	41.0
41	34.2193341972474	38.0	34.0	40.0	17.0	41.0
42	34.06192254837076	38.0	33.0	40.0	15.0	41.0
43	33.97802290963312	38.0	33.0	40.0	15.0	41.0
44	33.84737929840584	38.0	33.0	40.0	15.0	41.0
45	33.7670132059167	38.0	33.0	40.0	15.0	41.0
46	33.61651975615681	38.0	33.0	40.0	15.0	41.0
47	33.524567051533495	38.0	33.0	40.0	15.0	41.0
48	33.35394093705942	37.0	33.0	40.0	13.0	41.0
49	33.30150553292076	37.0	33.0	40.0	13.0	41.0
50	33.200068533699415	37.0	32.0	40.0	12.0	41.0
51	31.850796722065926	35.0	30.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	2.0
11	9.0
12	16.0
13	26.0
14	40.0
15	89.0
16	183.0
17	345.0
18	725.0
19	1339.0
20	2304.0
21	3711.0
22	6041.0
23	9853.0
24	16975.0
25	27984.0
26	36377.0
27	34978.0
28	29873.0
29	26891.0
30	27078.0
31	30642.0
32	37580.0
33	49132.0
34	77657.0
35	97361.0
36	104495.0
37	149847.0
38	255175.0
39	376822.0
40	135.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.554749663208874	19.05550303521649	23.231713007653404	21.158034293921233
2	41.32289987312004	21.879419159087234	21.472348931992773	15.325332035799951
3	28.41476993835529	21.626585376105393	34.52075210392046	15.437892581618861
4	23.954095244744384	23.875017899264012	35.04259134323914	17.12829551275247
5	21.675955286391787	27.702860106476578	33.75277572168764	16.868408885443998
6	22.0603709226189	32.47507104493944	32.94184110582899	12.522716926612661
7	75.36961534926895	4.814243041015496	16.792109933183205	3.024031676532337
8	77.45348150480626	3.566530762868413	16.088891485222153	2.8910962471031687
9	71.85587405757258	6.098786839534968	17.947921512528772	4.097417590363677
10	32.66193579916919	24.30524140318831	28.029784375313906	15.003038422328594
11	26.210221779895686	24.764673656344105	31.659933218825536	17.36517134493467
12	21.50654454084915	22.14215542046707	37.91594861824806	18.435351420435723
13	21.243665797765747	22.944398652408047	38.59921962770956	17.21271592211665
14	19.93354653345577	24.839832754976353	37.33212983787719	17.894490873690682
15	19.701871283453816	24.540264973224126	38.47184098471955	17.286022758602513
16	21.605996769939782	22.93186026249404	37.79897113961853	17.663171827947643
17	21.87899171397653	22.926873402869155	35.823462319644875	19.370672563509437
18	22.059017346435	23.077262840985433	36.18023650537975	18.683483307199815
19	21.812880203520866	25.13897309161787	35.22838748469212	17.81975922016914
20	22.108244775017827	24.758475702238886	35.34486627735916	17.788413245384127
21	21.746127525399146	24.656601284187595	35.56243583870786	18.0348353517054
22	20.760724063521195	23.518742399491625	35.326343655895286	20.394189881091897
23	19.763850824505997	25.034391521198785	35.69081185362285	19.51094580067237
24	19.82718394174208	25.036457505900522	36.413407813269174	18.72295073908822
25	20.778890480726144	25.500591655274068	34.90253182863155	18.817986035368232
26	19.79583796695707	25.789473309258675	35.020150474927135	19.394538248857117
27	20.50097991791629	24.788183137432863	35.34401138713775	19.366825557513096
28	19.639820501549845	24.929881191631477	36.2356618880678	19.194636418750875
29	20.503544588580517	24.751779062171178	35.22753259447071	19.51714375477759
30	20.629640896238413	24.83741056601569	35.39245516635095	19.140493371394946
31	20.905342992642957	25.056119980992943	34.11895369985802	19.919583326506086
32	20.813086089582523	25.550673974078304	33.64513079464183	19.99110914169734
33	21.066133595119716	25.206010733146726	32.13354240148637	21.594313270247184
34	20.722396485261335	25.649841239761802	32.00730361212491	21.620458662851956
35	21.145353422303657	25.777219882751805	31.772208801237312	21.305217893707223
36	21.173636040461954	26.968865610544785	30.816156570294417	21.041341778698843
37	21.823637572140267	26.750583640678244	31.124202013408954	20.30157677377254
38	22.083951644559445	26.79603530411651	30.407162840201785	20.712850211122262
39	22.709232600668667	25.66009992241871	29.73557533043288	21.89509214647974
40	22.04049472497113	25.77907214489819	31.199218630337633	20.981214499793047
41	20.66405022765014	26.11974589812986	31.217385047542585	21.998818826677418
42	20.66226920635554	27.092682210945583	31.06585575579776	21.179192826901115
43	21.264753089893844	26.07799875898436	30.61582729507747	22.041420856044322
44	21.297808845121676	26.470892056573785	30.190305687370923	22.040993410933616
45	21.922876078675547	25.976480545191986	29.826763620716555	22.27387975541591
46	21.474842361805216	26.51506138467994	30.211820424609726	21.79827582890512
47	21.46522484681436	25.859075621451762	31.16459557637055	21.511103955363332
48	20.973947932911067	26.029982424881865	30.98428498050494	22.01178466170213
49	21.287051476502274	25.69322691849833	30.87899100156801	22.140730603431386
50	20.677087303526637	25.472594000522907	30.896373769403336	22.95394492654712
51	20.742700128019813	25.07941573952635	30.534185278932867	23.643698853520974
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2750.0
1	2652.5
2	2555.0
3	16903.0
4	31251.0
5	22094.5
6	12938.0
7	13202.5
8	13467.0
9	13735.0
10	14003.0
11	14168.0
12	14333.0
13	14343.5
14	14354.0
15	13982.5
16	13611.0
17	13244.5
18	12878.0
19	12741.0
20	12604.0
21	12429.5
22	12255.0
23	12513.5
24	12772.0
25	13462.0
26	15880.5
27	17609.0
28	19647.0
29	21685.0
30	23707.5
31	25730.0
32	28667.0
33	31604.0
34	36227.0
35	40850.0
36	43130.0
37	45410.0
38	50106.0
39	54802.0
40	59316.5
41	63831.0
42	68702.5
43	73574.0
44	80220.0
45	86866.0
46	95176.5
47	103487.0
48	106651.0
49	109815.0
50	109762.5
51	109710.0
52	101662.5
53	93615.0
54	83744.0
55	73873.0
56	67360.0
57	60847.0
58	55120.5
59	49394.0
60	46753.5
61	44113.0
62	39115.0
63	34117.0
64	29195.0
65	24273.0
66	20228.5
67	16184.0
68	13871.0
69	11558.0
70	10193.0
71	8828.0
72	7700.5
73	6573.0
74	5427.5
75	3906.5
76	3531.0
77	2556.0
78	1581.0
79	1182.5
80	784.0
81	639.5
82	495.0
83	427.0
84	359.0
85	247.0
86	135.0
87	105.0
88	75.0
89	61.0
90	47.0
91	51.0
92	55.0
93	31.5
94	8.0
95	15.5
96	23.0
97	19.5
98	16.0
99	21.5
100	27.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1403689.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.31095927639148
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.70556753241135	25.884469956210427
2	7.83854084458032	5.222186297202926
3	3.0140608633676957	3.0120377602861996
4	1.7573701374868622	2.3415874033348545
5	1.2235595256799874	2.0378970766083473
6	0.8677732809076905	1.7343816252874023
7	0.6773934033511751	1.5795236851189054
8	0.5876665467207374	1.5660589124729674
9	0.4810493628833602	1.442179416324751
>10	5.259315610071309	38.73989549316184
>50	0.5439969346852476	11.527346157277082
>100	0.03918465232711546	2.100494014629716
>500	0.0023683028951638984	0.5239042487815607
>1k	0.0019377023687704624	0.9537854352561227
>5k	0.0	0.0
>10k+	2.1530026319671806E-4	1.3342525180468388
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18604	1.3253648065917734	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2338	0.1665611114712732	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCC	2140	0.15245542281801738	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCT	1544	0.1099958751546817	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT	1520	0.1082860947118628	TruSeq Adapter, Index 14 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.849634071364811E-4	0.0	0.0	0.0407497672205168	0.0
2	2.849634071364811E-4	0.0	0.0	0.19826329051520672	0.0
3	2.849634071364811E-4	0.0	0.0	0.3824208923771576	0.0
4	2.849634071364811E-4	0.0	0.0	0.6191542428557892	0.0
5	2.849634071364811E-4	0.0	0.0	1.2229917025779926	0.0
6	2.849634071364811E-4	0.0	0.0	1.8496262348711145	0.0
7	2.849634071364811E-4	0.0	0.0	2.3465311760653536	0.0
8	2.849634071364811E-4	0.0	0.0	2.9683213304371554	0.0
9	2.849634071364811E-4	0.0	0.0	3.3434044150805486	0.0
10	2.849634071364811E-4	0.0	0.0	4.028029000725945	0.0
11	2.849634071364811E-4	0.0	0.0	4.93293030008784	0.0
12	2.849634071364811E-4	0.0	0.0	5.573456798478865	0.0
13	2.849634071364811E-4	0.0	0.0	5.86091363542779	0.0
14	2.849634071364811E-4	0.0	0.0	5.985157680939296	0.0
15	2.849634071364811E-4	0.0	0.0	6.159341563551471	0.0
16	2.849634071364811E-4	0.0	0.0	6.518894142505926	0.0
17	2.849634071364811E-4	0.0	0.0	6.959376329087141	0.0
18	2.849634071364811E-4	0.0	0.0	7.425932667421344	0.0
19	2.849634071364811E-4	0.0	0.0	7.697289071867059	0.0
20	2.849634071364811E-4	0.0	0.0	7.969357884830615	0.0
21	2.849634071364811E-4	0.0	0.0	8.30988915635871	0.0
22	2.849634071364811E-4	0.0	0.0	8.664098671429356	0.0
23	3.5620425892060137E-4	0.0	0.0	9.049297957026093	0.0
24	3.5620425892060137E-4	0.0	0.0	9.351929095405037	0.0
25	3.5620425892060137E-4	0.0	0.0	9.623641704109671	0.0
26	3.5620425892060137E-4	0.0	0.0	9.890866139151907	0.0
27	4.2744511070472163E-4	0.0	0.0	10.162293784449405	0.0
28	4.2744511070472163E-4	0.0	0.0	10.4606504717213	0.0
29	4.2744511070472163E-4	0.0	0.0	10.76306788754489	0.0
30	4.2744511070472163E-4	0.0	0.0	11.097258723264199	0.0
31	4.2744511070472163E-4	0.0	0.0	11.416061534998137	0.0
32	4.2744511070472163E-4	0.0	0.0	11.723964496409105	0.0
33	4.2744511070472163E-4	0.0	0.0	12.024458409234525	0.0
34	4.2744511070472163E-4	0.0	0.0	12.33093655360981	0.0
35	4.2744511070472163E-4	0.0	0.0	12.638625792465424	0.0
36	4.986859624888419E-4	0.0	0.0	12.93612758951591	0.0
37	4.986859624888419E-4	0.0	0.0	13.24766383436787	0.0
38	5.699268142729622E-4	0.0	0.0	13.561408545625135	0.0
39	5.699268142729622E-4	0.0	0.0	13.884699531021473	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	35	1.2121927E-7	45.0	21
CGTTCTA	20	7.033821E-4	45.0	27
TCGTCGG	20	7.033821E-4	45.0	2
CGTTTTT	17675	0.0	43.536068	1
TGCGACG	65	9.094947E-12	38.07692	1
GCGAGAC	435	0.0	37.75862	21
ACACGAC	425	0.0	37.588234	26
GACACGA	425	0.0	37.588234	25
TAGCACG	60	1.5643309E-10	37.499996	1
ATACGCG	30	1.1400509E-4	37.499996	1
TATCGCG	60	1.5643309E-10	37.499996	1
GTTTTTT	20770	0.0	37.481945	2
TACGCAT	85	0.0	37.058823	16
CGAGACA	450	0.0	36.5	22
TACGAAT	105	0.0	36.42857	12
CGACCAA	445	0.0	36.404495	29
CACGACC	445	0.0	36.404495	27
ACGACCA	455	0.0	36.0989	28
GCAACCG	50	4.8803486E-8	36.000004	1
CGCGTAA	50	4.8803486E-8	36.000004	31
>>END_MODULE