FastQCFastQC Report
Sat 18 Jun 2016
SRR3553666_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553666_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1646702
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28040.17027974703376808No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26280.15959171726274698No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC24050.1460494977233282TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT20850.12661671632147165TruSeq Adapter, Index 20 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCC18070.10973448747860877No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC17540.10651593305892627TruSeq Adapter, Index 23 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAC253.8915437E-545.08
GCGCCTA207.034178E-445.09
ATTCGGA253.8915437E-545.010
TAGATCG207.034178E-445.041
GATTACG207.034178E-445.01
CCGCACG207.034178E-445.01
CGCATCG700.041.78571721
ACGCATT1950.041.53846417
TTGAGCG1350.040.0000041
GTATGCG451.9292202E-840.01
ATCGCCA750.039.024
TAACCCG356.249935E-638.5714337
TACGAAT1400.038.5714312
TTACGCG356.249935E-638.571431
GCCGCAT950.037.89473719
TAGTGCG900.037.51
ATCGATC301.1401365E-437.49999619
ACGCGCT301.1401365E-437.49999626
CCCGCTA850.037.05882324
ACAACGA10550.036.89573313
Produced by FastQC (version 0.11.5)