##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553666_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1646702
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10447366918847	33.0	31.0	34.0	30.0	34.0
2	32.24641313364531	34.0	31.0	34.0	30.0	34.0
3	32.27335911415666	34.0	31.0	34.0	30.0	34.0
4	35.80396878123668	37.0	35.0	37.0	35.0	37.0
5	35.74389051570958	37.0	35.0	37.0	35.0	37.0
6	35.72140982399973	37.0	35.0	37.0	33.0	37.0
7	35.94728554407537	37.0	35.0	37.0	35.0	37.0
8	35.87776841225674	37.0	35.0	37.0	35.0	37.0
9	37.655246668796174	39.0	38.0	39.0	35.0	39.0
10	37.032547479750434	39.0	37.0	39.0	33.0	39.0
11	36.9711052758787	39.0	37.0	39.0	33.0	39.0
12	36.93227675681453	39.0	37.0	39.0	33.0	39.0
13	36.90066144329697	39.0	37.0	39.0	33.0	39.0
14	38.06099889354601	40.0	37.0	41.0	33.0	41.0
15	38.157244601634055	40.0	37.0	41.0	33.0	41.0
16	38.21655162864926	40.0	37.0	41.0	33.0	41.0
17	38.16978906930337	40.0	37.0	41.0	33.0	41.0
18	38.140543340567994	40.0	37.0	41.0	33.0	41.0
19	38.06446825230066	40.0	37.0	41.0	34.0	41.0
20	38.02997870895888	40.0	37.0	41.0	33.0	41.0
21	37.917846094800396	40.0	36.0	41.0	33.0	41.0
22	37.903610975149114	40.0	36.0	41.0	33.0	41.0
23	37.8180599768507	40.0	36.0	41.0	33.0	41.0
24	37.80319086270619	40.0	36.0	41.0	33.0	41.0
25	37.74037014590375	40.0	36.0	41.0	33.0	41.0
26	37.67107649107125	40.0	36.0	41.0	33.0	41.0
27	37.62333986355758	40.0	36.0	41.0	33.0	41.0
28	37.54837365837899	40.0	36.0	41.0	33.0	41.0
29	37.48092490323082	40.0	36.0	41.0	33.0	41.0
30	37.38696801242726	40.0	36.0	41.0	32.0	41.0
31	37.264808083065425	39.0	35.0	41.0	32.0	41.0
32	37.151551403957725	39.0	35.0	41.0	32.0	41.0
33	37.06593603457092	39.0	35.0	41.0	31.0	41.0
34	36.97787092017864	39.0	35.0	41.0	31.0	41.0
35	36.840686414421064	39.0	35.0	41.0	31.0	41.0
36	36.741128024378426	39.0	35.0	41.0	31.0	41.0
37	36.67514583695168	39.0	35.0	41.0	30.0	41.0
38	36.58666473958251	39.0	35.0	41.0	30.0	41.0
39	36.54227055046997	39.0	35.0	41.0	30.0	41.0
40	36.431030022432715	39.0	35.0	41.0	30.0	41.0
41	36.30923445772216	39.0	35.0	41.0	30.0	41.0
42	36.22057057075293	39.0	35.0	41.0	30.0	41.0
43	36.10330345138343	39.0	35.0	40.0	29.0	41.0
44	36.000960708130556	38.0	35.0	40.0	29.0	41.0
45	35.9165483493674	38.0	35.0	40.0	29.0	41.0
46	35.73991711918732	38.0	35.0	40.0	28.0	41.0
47	35.63082209167172	38.0	34.0	40.0	28.0	41.0
48	35.51318574945558	38.0	34.0	40.0	28.0	41.0
49	35.43129297225606	38.0	34.0	40.0	27.0	41.0
50	35.32740532288174	38.0	34.0	40.0	27.0	41.0
51	33.862353965684136	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	7.0
11	10.0
12	13.0
13	20.0
14	56.0
15	108.0
16	186.0
17	364.0
18	577.0
19	995.0
20	1627.0
21	2600.0
22	3868.0
23	5836.0
24	8051.0
25	10790.0
26	13585.0
27	16533.0
28	19598.0
29	23637.0
30	29257.0
31	36707.0
32	47323.0
33	64439.0
34	103119.0
35	126332.0
36	136366.0
37	196912.0
38	328343.0
39	469270.0
40	167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.685193799485276	18.64308174763861	24.683701118963842	10.988023333912269
2	33.072711395261564	23.006773538867385	25.658437288592594	18.262077777278463
3	32.289388122441096	25.459676371316732	25.59843857601436	16.652496930227816
4	27.312592077983748	27.361113304046512	25.760823755603624	19.565470862366112
5	25.00075909302351	31.453110520300577	24.057965557824062	19.48816482885185
6	25.51742816854537	36.42547346150062	23.669795749321977	14.387302620632028
7	83.91032500112345	5.566580960003692	6.552976798473555	3.970117240399295
8	84.84455596701771	4.560327248038807	6.567065564989901	4.02805121995358
9	79.36997708146343	7.599917896498577	8.284680531146497	4.745424490891491
10	35.20831334388371	33.95210548113745	16.123075091910984	14.716506083067854
11	28.119356143370204	27.832965527460342	25.64416633975061	18.40351198941885
12	25.257636172179303	25.04843013489994	30.43380040833132	19.26013328458944
13	23.86612756892261	26.633234185663223	30.62399875630199	18.876639489112176
14	21.870016554300655	28.536189304440025	29.3735600005344	20.220234140724916
15	20.874572326990553	27.74904020278107	31.505821939853114	19.87056553037526
16	23.762951645167128	27.305972786818742	29.59236097363093	19.338714594383198
17	23.7276082739925	25.184763241922337	28.657279823550347	22.430348660534815
18	25.48943281783832	24.446013911442385	28.889683743628176	21.17486952709112
19	23.44874786087586	28.07356765219208	27.70234079997474	20.775343686957324
20	25.63487504114284	27.311195346820494	26.579429672156834	20.474499939879834
21	24.109644610864624	28.23819974713093	28.304939205757933	19.34721643624651
22	23.89473019404847	24.625463502200155	27.791002865120706	23.688803438630668
23	21.439701901133297	28.28435260296034	28.200609460606717	22.075336035299646
24	20.71668097810047	27.436293877094947	29.428275425669003	22.41874971913558
25	22.03981048179938	28.322914528554648	28.00348818426163	21.63378680538434
26	22.442494148910974	28.255446340625078	27.455423021287395	21.84663648917655
27	22.807526802056476	27.06488484255196	27.832661890250936	22.294926465140627
28	20.527818633851176	27.99583652658465	28.904987058982133	22.571357780582034
29	22.03762429389167	27.209537609112033	28.094214982431552	22.65862311456475
30	23.994505381058627	26.892358180168603	27.386861739403972	21.7262746993688
31	23.34350720409643	28.485421163027674	26.35127667301066	21.81979495986523
32	23.05584131190707	27.785598122793314	26.871103575510325	22.287456989789288
33	22.496359389859247	27.41546436452983	25.69736357883819	24.39081266677274
34	21.89825481477523	27.921627592606313	27.539591255734187	22.64052633688427
35	22.648360176886893	27.142312330949984	27.213606347718045	22.995721144445078
36	23.11845130448618	29.226963955834144	26.373017097203988	21.28156764247569
37	21.88082603895544	28.278219131330378	27.453358288263452	22.38759654145073
38	20.83254893720904	29.434591079624607	27.18676481840673	22.54609516475962
39	22.50826196846788	26.626129074963167	27.370283147770515	23.495325808798434
40	23.207052642190266	25.599288760200693	29.41042155775605	21.78323703985299
41	21.026573114018202	27.745518011151987	28.285081332262912	22.942827542566903
42	22.693966485739374	27.688009123690865	27.568072425976286	22.049951964593472
43	22.15039515346432	26.617505778216096	27.579610639933637	23.652488428385947
44	22.233531021399138	27.29589203146653	26.60177737076897	23.868799576365365
45	22.99329204676985	25.40016347827354	27.078184152323857	24.528360322632754
46	22.72991713133281	26.784202606178898	27.125551556990885	23.360328705497412
47	21.440977177412794	27.17528733189126	29.262246599566893	22.121488891129058
48	21.314724825742605	27.740416906034	27.827317875365427	23.117540392857965
49	22.37854815261049	26.825497266657838	28.38267033136536	22.41328424936631
50	21.310716814578473	26.327957335328435	28.655215090526397	23.706110759566698
51	21.05001390658419	25.76507467653528	27.526352673404176	25.658558743476355
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3516.0
1	3708.5
2	3901.0
3	5005.0
4	6109.0
5	4716.0
6	3323.0
7	3373.5
8	3424.0
9	3611.0
10	3798.0
11	3728.0
12	3658.0
13	3764.5
14	3871.0
15	4151.0
16	4431.0
17	4561.0
18	4691.0
19	4908.0
20	5125.0
21	6203.0
22	7281.0
23	8121.0
24	8961.0
25	10699.0
26	14188.0
27	15939.0
28	19078.5
29	22218.0
30	27575.0
31	32932.0
32	36291.0
33	39650.0
34	45706.0
35	51762.0
36	54825.5
37	57889.0
38	62591.5
39	67294.0
40	70660.5
41	74027.0
42	84921.5
43	95816.0
44	100672.5
45	105529.0
46	119051.5
47	132574.0
48	140978.5
49	149383.0
50	146757.0
51	144131.0
52	134392.0
53	124653.0
54	112266.0
55	99879.0
56	89094.0
57	78309.0
58	71368.0
59	64427.0
60	58145.5
61	51864.0
62	47337.0
63	42810.0
64	37155.0
65	31500.0
66	27518.0
67	23536.0
68	21115.5
69	18695.0
70	15748.0
71	12801.0
72	12077.5
73	11354.0
74	10186.5
75	7752.0
76	6485.0
77	4937.0
78	3389.0
79	2672.0
80	1955.0
81	1658.5
82	1362.0
83	939.0
84	516.0
85	343.5
86	171.0
87	116.0
88	61.0
89	57.5
90	54.0
91	60.5
92	67.0
93	51.5
94	36.0
95	49.0
96	62.0
97	44.5
98	27.0
99	13.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1646702.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.95284152961481
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.43022036673908	16.85432211562456
2	10.790837726366332	4.953607766101508
3	3.982348282347198	2.742186271213469
4	1.9834117109415288	1.8209973875689234
5	1.132508991398097	1.2997149705212212
6	0.7994512314687476	1.1009806455934543
7	0.5477005929930011	0.8799899441651087
8	0.4291373356725416	0.787993700810637
9	0.3578685059536114	0.7392689195033931
>10	4.299462388745882	24.051842822010947
>50	1.7945606443151505	29.5318819447412
>100	0.4453055818865074	13.496706166699845
>500	0.0047910941149641685	0.6959219057169991
>1k	0.0023955470574820842	1.0445854397286674
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2804	0.17027974703376808	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2628	0.15959171726274698	No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	2405	0.1460494977233282	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	2085	0.12661671632147165	TruSeq Adapter, Index 20 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCC	1807	0.10973448747860877	No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	1754	0.10651593305892627	TruSeq Adapter, Index 23 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.214548837616035E-4	0.0	0.0	0.13335746237024063	0.0
2	1.214548837616035E-4	0.0	0.0	0.5001512113302832	0.0
3	1.214548837616035E-4	0.0	0.0	0.7171303611703879	0.0
4	1.214548837616035E-4	0.0	0.0	1.0001809677768048	0.0
5	1.214548837616035E-4	0.0	0.0	1.767472195940735	0.0
6	1.214548837616035E-4	0.0	0.0	2.2990194947233924	0.0
7	1.214548837616035E-4	0.0	0.0	2.791701230702337	0.0
8	1.214548837616035E-4	0.0	0.0	3.6297399286574015	0.0
9	1.214548837616035E-4	0.0	0.0	3.9954405836635893	0.0
10	1.8218232564240524E-4	0.0	0.0	4.807609391377432	0.0
11	1.8218232564240524E-4	0.0	0.0	5.870461079175224	0.0
12	1.8218232564240524E-4	0.0	0.0	6.602894755699574	0.0
13	1.8218232564240524E-4	0.0	0.0	6.9105399762677155	0.0
14	1.8218232564240524E-4	0.0	0.0	7.025861388399358	0.0
15	1.8218232564240524E-4	0.0	0.0	7.2613016805712265	0.0
16	1.8218232564240524E-4	0.0	0.0	7.781128583070889	0.0
17	1.8218232564240524E-4	0.0	0.0	8.402188131185849	0.0
18	2.42909767523207E-4	0.0	0.0	9.10735518630572	0.0
19	5.465469769272157E-4	0.0	0.0	9.479250040383748	0.0
20	6.072744188080174E-4	0.0	0.0	9.844950695389937	0.0
21	6.680018606888193E-4	0.0	0.0	10.305264704846413	0.0
22	6.680018606888193E-4	0.0	0.0	10.789566053845808	0.0
23	6.680018606888193E-4	0.0	0.0	11.294028913549628	0.0
24	7.894567444504228E-4	0.0	0.0	11.682137994609832	0.0
25	8.501841863312245E-4	0.0	0.0	12.03490370449541	0.0
26	8.501841863312245E-4	0.0	0.0	12.372791191120191	0.0
27	8.501841863312245E-4	0.0	0.0	12.708128125185977	0.0
28	9.71639070092828E-4	0.0	0.0	13.053849451813383	0.0
29	9.71639070092828E-4	0.0	0.0	13.434428330080367	0.0
30	9.71639070092828E-4	0.0	0.0	13.842334557193713	0.0
31	9.71639070092828E-4	0.0	0.0	14.235058923836856	0.0
32	0.0010323665119736298	0.0	0.0	14.601063216052449	0.0
33	0.0010323665119736298	0.0	0.0	14.97459771106126	0.0
34	0.0010930939538544315	0.0	0.0	15.336958356764004	0.0
35	0.0010930939538544315	0.0	0.0	15.733083460152475	0.0
36	0.0010930939538544315	0.0	0.0	16.10090957562449	0.0
37	0.0010930939538544315	0.0	0.0	16.464545497606732	0.0
38	0.0010930939538544315	0.0	0.0	16.845185103315597	0.0
39	0.0010930939538544315	0.0	0.0	17.24070293228526	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGAC	25	3.8915437E-5	45.0	8
GCGCCTA	20	7.034178E-4	45.0	9
ATTCGGA	25	3.8915437E-5	45.0	10
TAGATCG	20	7.034178E-4	45.0	41
GATTACG	20	7.034178E-4	45.0	1
CCGCACG	20	7.034178E-4	45.0	1
CGCATCG	70	0.0	41.785717	21
ACGCATT	195	0.0	41.538464	17
TTGAGCG	135	0.0	40.000004	1
GTATGCG	45	1.9292202E-8	40.0	1
ATCGCCA	75	0.0	39.0	24
TAACCCG	35	6.249935E-6	38.57143	37
TACGAAT	140	0.0	38.57143	12
TTACGCG	35	6.249935E-6	38.57143	1
GCCGCAT	95	0.0	37.894737	19
TAGTGCG	90	0.0	37.5	1
ATCGATC	30	1.1401365E-4	37.499996	19
ACGCGCT	30	1.1401365E-4	37.499996	26
CCCGCTA	85	0.0	37.058823	24
ACAACGA	1055	0.0	36.895733	13
>>END_MODULE