##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553665_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	700016
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.873574318301294	33.0	31.0	34.0	30.0	34.0
2	32.013732543256154	33.0	31.0	34.0	30.0	34.0
3	32.07545684670065	33.0	31.0	34.0	30.0	34.0
4	35.6394639551096	37.0	35.0	37.0	33.0	37.0
5	35.56353569061279	37.0	35.0	37.0	33.0	37.0
6	35.531042147608055	37.0	35.0	37.0	33.0	37.0
7	35.75201709675208	37.0	35.0	37.0	35.0	37.0
8	35.67766165344792	37.0	35.0	37.0	35.0	37.0
9	37.47228634774062	39.0	37.0	39.0	35.0	39.0
10	36.8581418139014	39.0	37.0	39.0	32.0	39.0
11	36.81654133619803	39.0	37.0	39.0	33.0	39.0
12	36.70542387602569	39.0	35.0	39.0	33.0	39.0
13	36.60586472309205	39.0	35.0	39.0	32.0	39.0
14	37.69884974057736	40.0	36.0	41.0	33.0	41.0
15	37.821681218715	40.0	36.0	41.0	33.0	41.0
16	37.89637379717035	40.0	36.0	41.0	33.0	41.0
17	37.86654019336701	40.0	36.0	41.0	33.0	41.0
18	37.798651745102966	39.0	36.0	41.0	33.0	41.0
19	37.73564747091495	39.0	36.0	41.0	33.0	41.0
20	37.67471029233617	39.0	36.0	41.0	33.0	41.0
21	37.58973652030811	39.0	35.0	41.0	33.0	41.0
22	37.54756319855546	39.0	35.0	41.0	33.0	41.0
23	37.47507485543188	39.0	35.0	41.0	33.0	41.0
24	37.457649539439096	39.0	35.0	41.0	33.0	41.0
25	37.3806013005417	39.0	35.0	41.0	32.0	41.0
26	37.32285262051153	39.0	35.0	41.0	32.0	41.0
27	37.29643036730589	39.0	35.0	41.0	32.0	41.0
28	37.24013165413362	39.0	35.0	41.0	32.0	41.0
29	37.172507485543186	39.0	35.0	41.0	32.0	41.0
30	37.04029050764554	39.0	35.0	41.0	31.0	41.0
31	36.939394242417315	39.0	35.0	41.0	31.0	41.0
32	36.89314101391968	39.0	35.0	41.0	31.0	41.0
33	36.79406042147608	39.0	35.0	41.0	31.0	41.0
34	36.762159722063494	39.0	35.0	41.0	31.0	41.0
35	36.61076318255583	39.0	35.0	41.0	31.0	41.0
36	36.513563975680555	39.0	35.0	41.0	30.0	41.0
37	36.44426127402802	39.0	35.0	40.0	30.0	41.0
38	36.354211903727915	39.0	35.0	40.0	30.0	41.0
39	36.30054170190396	39.0	35.0	40.0	30.0	41.0
40	36.18957280976435	38.0	35.0	40.0	30.0	41.0
41	36.03989337386574	38.0	35.0	40.0	30.0	41.0
42	35.99392585312336	38.0	35.0	40.0	30.0	41.0
43	35.91052347374917	38.0	35.0	40.0	29.0	41.0
44	35.79931601563393	38.0	35.0	40.0	29.0	41.0
45	35.739514525382276	38.0	35.0	40.0	29.0	41.0
46	35.61844443555576	38.0	35.0	40.0	29.0	41.0
47	35.51454681035862	38.0	34.0	40.0	28.0	41.0
48	35.383509805490164	38.0	34.0	40.0	28.0	41.0
49	35.375828552490226	38.0	34.0	40.0	28.0	41.0
50	35.28169641836758	37.0	34.0	40.0	27.0	41.0
51	33.934322929761606	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	4.0
11	5.0
12	3.0
13	7.0
14	16.0
15	25.0
16	68.0
17	151.0
18	272.0
19	538.0
20	830.0
21	1243.0
22	1950.0
23	2705.0
24	3588.0
25	4718.0
26	5794.0
27	7091.0
28	8575.0
29	10461.0
30	13326.0
31	17253.0
32	22317.0
33	30638.0
34	50550.0
35	63716.0
36	60352.0
37	85382.0
38	137320.0
39	171058.0
40	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.52474229160476	21.875928550204566	24.597152065095653	11.002177093095016
2	30.949721149230875	24.401156544993256	25.377991360197484	19.27113094557839
3	30.71386939727092	25.766125345706385	26.57296404653608	16.947041210486617
4	27.872648625174286	27.1939498525748	25.359134648350896	19.574266873900026
5	21.938784256359856	31.193572718337865	25.75012571141231	21.11751731388997
6	23.366037347717768	36.98015473932024	25.04357043267583	14.610237480286164
7	79.4486126031405	7.15169367557313	8.348809170076112	5.050884551210258
8	79.73803455921008	5.407304975886265	8.369094420698955	6.485566044204703
9	74.05859294644695	8.172098923453177	10.846609220360676	6.922698909739205
10	37.40957349546296	25.828838197984044	19.5958378094215	17.165750497131494
11	29.538610546044662	26.781245000114286	23.25189709949487	20.428247354346183
12	25.62912847706338	23.204183904367902	30.75343992137323	20.413247697195494
13	22.986331740988778	26.81352997645768	30.98586318027017	19.214275102283377
14	19.918116157344976	29.79117620168682	28.85234051793102	21.43836712303719
15	18.49914859088935	27.34223217755023	33.64937372860049	20.50924550295993
16	20.66381339855089	25.310421476080545	32.14126534250646	21.884499782862104
17	20.306678704486757	25.466989325958263	27.116951612534574	27.109380357020413
18	21.323798313181413	26.776102260519757	29.715606500422847	22.18449292587598
19	22.42934447212635	28.436064318529862	26.474537724852006	22.660053484491783
20	22.84862060295765	27.183521519508126	27.242091609334647	22.725766268199585
21	22.610483188955683	28.54363328838198	28.037787707709537	20.8080958149528
22	20.083540947635484	26.75510274050879	25.970977806221573	27.190378505634154
23	20.15596786359169	28.44134991200201	27.009954058192953	24.39272816621334
24	21.259371214372244	26.173830312450004	29.290330506731276	23.276467966446482
25	20.91623620031542	27.636796873214326	26.33525519416699	25.11171173230326
26	18.995280107883247	28.977623368608718	26.802815935635753	25.22428058787228
27	21.05137596854929	27.569084135219768	27.486943155585013	23.892596740645928
28	18.502005668441864	26.19911544878974	30.615728783342096	24.6831500994263
29	19.7434058650088	26.025262279719318	28.44663550547416	25.78469634979772
30	20.337963703686775	26.946669790404794	28.658202098237755	24.05716440767068
31	21.555507302690224	26.74167447601198	26.76824529725035	24.934572924047448
32	21.296798930310164	26.412396288084842	27.324089735091768	24.966715046513222
33	19.17627597083495	27.37066009919773	28.3622088637974	25.090855066169915
34	18.773570889808234	24.42044181847272	29.700321135516905	27.105666156202147
35	18.89828232497543	23.636316884185504	28.79162761994011	28.673773170898954
36	21.15294507554113	25.915550501702818	29.435327192521317	23.49617723023474
37	18.647430915864778	25.70969806404425	31.60370620100112	24.03916481908985
38	19.362843135014057	28.0180738725972	28.492063038559117	24.127019953829627
39	21.300084569495553	24.924573152613654	28.749485726040547	25.025856551850246
40	21.404510754039908	23.852169093277865	28.805627299947428	25.937692852734795
41	18.54829032479258	24.6892928161642	28.1227857648968	28.639631094146417
42	20.379962743708717	24.059735777467946	29.701892528170788	25.858408950652557
43	20.896808072958333	23.07604397613769	30.654870745811525	25.372277205092452
44	20.609814632808394	23.720457818107015	29.472754908459237	26.196972640625354
45	21.438938538547692	23.847312061438597	27.499085735183197	27.214663664830518
46	22.151636534022078	24.936287170578957	27.61336883728372	25.298707458115246
47	19.487840277936506	23.268182441544194	31.19628694201275	26.047690338506545
48	19.447412630568444	23.28803913053416	30.08331238143128	27.181235857466113
49	20.471674933144385	22.038496262942562	32.59825489703093	24.89157390688213
50	19.608694658407806	22.17306461566593	31.43571004091335	26.78253068501291
51	19.293273296610362	22.7130522730909	27.633939795661817	30.35973463463692
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3606.0
1	3373.0
2	3140.0
3	2972.0
4	2804.0
5	2130.5
6	1457.0
7	1574.0
8	1691.0
9	1830.5
10	1970.0
11	1933.0
12	1896.0
13	2050.0
14	2204.0
15	2154.0
16	2104.0
17	2104.5
18	2105.0
19	2144.5
20	2184.0
21	2306.5
22	2429.0
23	2479.5
24	2530.0
25	2966.0
26	3769.0
27	4136.0
28	5870.0
29	7604.0
30	7793.5
31	7983.0
32	9278.0
33	10573.0
34	12719.5
35	14866.0
36	16316.5
37	17767.0
38	19438.0
39	21109.0
40	25549.0
41	29989.0
42	37152.0
43	44315.0
44	53941.0
45	63567.0
46	72339.0
47	81111.0
48	78667.5
49	76224.0
50	66490.0
51	56756.0
52	49600.0
53	42444.0
54	39066.5
55	35689.0
56	33683.5
57	31678.0
58	29489.5
59	27301.0
60	24645.0
61	21989.0
62	20152.5
63	18316.0
64	16600.0
65	14884.0
66	12700.5
67	10517.0
68	9298.5
69	8080.0
70	7193.5
71	6307.0
72	5514.5
73	4722.0
74	4260.5
75	2841.0
76	1883.0
77	1626.5
78	1370.0
79	1068.5
80	767.0
81	576.0
82	385.0
83	304.5
84	224.0
85	139.0
86	54.0
87	43.5
88	33.0
89	31.5
90	30.0
91	22.0
92	14.0
93	9.0
94	4.0
95	3.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	700016.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.283602883912053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.21655573912139	20.425443841692097
2	11.882473969072644	6.721583500393459
3	4.538219520660189	3.850715961671116
4	2.3288249673052706	2.6347024224560696
5	1.3709497508578412	1.938769916353067
6	0.8953714059865786	1.519459756832063
7	0.608576570184819	1.2048916624892458
8	0.4784792383926977	1.0826493413516598
9	0.37399659638171207	0.9520174090795576
>10	3.9554978473490543	26.059141705980053
>50	1.1735918490551835	22.885760279799037
>100	0.1662757949050532	6.851245519098802
>500	0.0055933753637785475	0.9972403633534382
>1k	0.0055933753637785475	2.876378319450356
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	3255	0.4649893716715046	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3021	0.4315615643071016	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	2579	0.3684201503965624	No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	2288	0.326849672007497	No Hit
TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1558	0.22256634134076936	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1556	0.22228063358551803	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1504	0.21485223194898403	No Hit
ACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1112	0.15885351191972755	No Hit
AAAACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTC	1058	0.15113940252794222	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCC	1053	0.15042513313981395	No Hit
ACTCTAGGGGGTTTTATTTTTCATGACTGTTGAGTTCTCTTTATCACAAAC	1016	0.14513953966766474	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	914	0.130568444149848	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	718	0.10256908413521977	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4285387762565427E-4	0.0	0.0	0.33813512833992365	0.0
2	1.4285387762565427E-4	0.0	0.0	1.59824918287582	0.0
3	1.4285387762565427E-4	0.0	0.0	2.1102374802861648	0.0
4	1.4285387762565427E-4	0.0	0.0	2.7802221663504834	0.0
5	1.4285387762565427E-4	0.0	0.0	4.818318438435693	0.0
6	1.4285387762565427E-4	0.0	0.0	6.113431692989875	0.0
7	1.4285387762565427E-4	0.0	0.0	7.2725480560444336	0.0
8	1.4285387762565427E-4	0.0	0.0	9.056792987588855	0.0
9	1.4285387762565427E-4	0.0	0.0	9.71177801650248	0.0
10	1.4285387762565427E-4	0.0	0.0	11.29059907202121	0.0
11	8.571232657539256E-4	0.0	0.0	13.21169801833101	0.0
12	8.571232657539256E-4	0.0	0.0	14.604666179058764	0.0
13	8.571232657539256E-4	0.0	0.0	15.153082215263652	0.0
14	8.571232657539256E-4	0.0	0.0	15.349506296998925	0.0
15	8.571232657539256E-4	0.0	0.0	15.740925921693218	0.0
16	8.571232657539256E-4	0.0	0.0	16.654476469109277	0.0
17	9.9997714337958E-4	0.0	0.0	17.7727366231629	0.0
18	9.9997714337958E-4	0.0	0.0	19.11099174876003	0.0
19	9.9997714337958E-4	0.0	0.0	19.692549884574067	0.0
20	9.9997714337958E-4	0.0	0.0	20.257679824461157	0.0
21	9.9997714337958E-4	0.0	0.0	20.98923453178213	0.0
22	9.9997714337958E-4	0.0	0.0	21.759931201572535	0.0
23	0.0012856848986308885	0.0	0.0	22.533627802793077	0.0
24	0.0012856848986308885	0.0	0.0	23.13418550433133	0.0
25	0.0012856848986308885	0.0	0.0	23.683172956046718	0.0
26	0.0012856848986308885	0.0	0.0	24.19916116203058	0.0
27	0.0012856848986308885	0.0	0.0	24.702578226783388	0.0
28	0.0012856848986308885	0.0	0.0	25.22842335032342	0.0
29	0.0012856848986308885	0.0	0.0	25.75312564284245	0.0
30	0.0012856848986308885	0.0	0.0	26.31625562844278	0.0
31	0.0012856848986308885	0.0	0.0	26.882956960983748	0.0
32	0.0012856848986308885	0.0	0.0	27.397230920436105	0.0
33	0.0012856848986308885	0.0	0.0	27.89964800804553	0.0
34	0.0014285387762565428	0.0	0.0	28.413921967497885	0.0
35	0.0015713926538821969	0.0	0.0	28.972480629014193	0.0
36	0.0017142465315078512	0.0	0.0	29.501039976229116	0.0
37	0.0017142465315078512	0.0	0.0	29.9887431144431	0.0
38	0.0017142465315078512	0.0	0.0	30.45744668693287	0.0
39	0.0017142465315078512	0.0	0.0	30.9381499851432	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCT	20	7.0313935E-4	45.000004	34
CTCGTCA	60	0.0	45.000004	36
GAATCGT	20	7.0313935E-4	45.000004	10
CCGTAGT	60	0.0	45.000004	27
GTTCGCG	20	7.0313935E-4	45.000004	2
ACGTGAC	20	7.0313935E-4	45.000004	25
ATAACGC	40	6.8084773E-9	45.000004	11
GCTAACC	20	7.0313935E-4	45.000004	29
ATCGCGT	20	7.0313935E-4	45.000004	32
TCGCGAC	20	7.0313935E-4	45.000004	17
GTTATCG	20	7.0313935E-4	45.000004	42
CTTACGT	20	7.0313935E-4	45.000004	22
CAGCGGA	25	3.889238E-5	45.0	37
GTACCAT	35	1.2110104E-7	45.0	9
ATATGCG	25	3.889238E-5	45.0	1
CGTTCGG	35	1.2110104E-7	45.0	2
CCTCGCA	25	3.889238E-5	45.0	22
ACGATAG	25	3.889238E-5	45.0	1
TCGCTAG	70	0.0	45.0	1
CATTTCG	35	1.2110104E-7	45.0	39
>>END_MODULE