SRR3553664_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553664_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	934705
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3283	0.3512338117373931	No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	2675	0.28618655083689504	RNA PCR Primer, Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	2522	0.26981774998528946	TruSeq Adapter, Index 10 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	2400	0.25676550355459743	Illumina PCR Primer Index 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1660	0.17759613995859655	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCC	1494	0.1598365259627369	No Hit
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1459	0.15609202903589903	Illumina PCR Primer Index 2 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1394	0.1491379633146287	No Hit
ACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1120	0.11982390165881213	TruSeq Adapter, Index 10 (95% over 23bp)
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	961	0.10281318704832006	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	20	7.032608E-4	45.000004	1
CGAACTT	20	7.032608E-4	45.000004	6
CTATCGG	20	7.032608E-4	45.000004	2
GCACGAC	20	7.032608E-4	45.000004	22
CGATTGT	40	6.8121153E-9	45.000004	10
CGACATA	20	7.032608E-4	45.000004	25
ATCGTAG	20	7.032608E-4	45.000004	1
TCGTCGA	20	7.032608E-4	45.000004	42
ACAGCGA	20	7.032608E-4	45.000004	26
ACTCGAC	20	7.032608E-4	45.000004	35
CGTAACA	20	7.032608E-4	45.000004	17
ATCGGTT	20	7.032608E-4	45.000004	17
CGCGTAC	20	7.032608E-4	45.000004	43
CCGTAGT	35	1.2116107E-7	45.0	27
CGGTCTA	35	1.2116107E-7	45.0	31
CCGCAAT	25	3.8902457E-5	45.0	28
ACGACGT	25	3.8902457E-5	45.0	23
ACGATAG	30	2.1650649E-6	44.999996	1
CCGTACT	30	2.1650649E-6	44.999996	44
TACGCAT	110	0.0	42.954544	16