##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553664_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	934705
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.986113265682757	33.0	31.0	34.0	30.0	34.0
2	32.132436437164664	33.0	31.0	34.0	30.0	34.0
3	32.173020364714	34.0	31.0	34.0	30.0	34.0
4	35.72198394145747	37.0	35.0	37.0	33.0	37.0
5	35.664286593096215	37.0	35.0	37.0	33.0	37.0
6	35.63659122396906	37.0	35.0	37.0	33.0	37.0
7	35.82911185882177	37.0	35.0	37.0	35.0	37.0
8	35.74996603206359	37.0	35.0	37.0	35.0	37.0
9	37.535083261563805	39.0	37.0	39.0	35.0	39.0
10	37.00112548879058	39.0	37.0	39.0	33.0	39.0
11	36.93978848941644	39.0	37.0	39.0	33.0	39.0
12	36.826300276557845	39.0	35.0	39.0	33.0	39.0
13	36.76813540100888	39.0	35.0	39.0	33.0	39.0
14	37.87878849476573	40.0	37.0	41.0	33.0	41.0
15	37.99686104171904	40.0	37.0	41.0	33.0	41.0
16	38.069799562428784	40.0	37.0	41.0	33.0	41.0
17	38.03677630910287	40.0	37.0	41.0	33.0	41.0
18	37.99729861293135	40.0	37.0	41.0	33.0	41.0
19	37.93164367367244	40.0	36.0	41.0	33.0	41.0
20	37.85819590138065	40.0	36.0	41.0	33.0	41.0
21	37.75395873564386	40.0	36.0	41.0	33.0	41.0
22	37.70629770890281	39.0	36.0	41.0	33.0	41.0
23	37.62424615252941	39.0	36.0	41.0	33.0	41.0
24	37.60548515306969	39.0	36.0	41.0	33.0	41.0
25	37.549534879988876	39.0	35.0	41.0	33.0	41.0
26	37.521345237267376	39.0	35.0	41.0	33.0	41.0
27	37.45988520442278	39.0	35.0	41.0	33.0	41.0
28	37.373610925372176	39.0	35.0	41.0	33.0	41.0
29	37.294285362761514	39.0	35.0	41.0	32.0	41.0
30	37.17894843827732	39.0	35.0	41.0	32.0	41.0
31	37.03103653024216	39.0	35.0	41.0	31.0	41.0
32	36.924606159162515	39.0	35.0	41.0	31.0	41.0
33	36.83345119583184	39.0	35.0	41.0	31.0	41.0
34	36.70642181222953	39.0	35.0	41.0	31.0	41.0
35	36.60717338625555	39.0	35.0	41.0	31.0	41.0
36	36.50446504512119	39.0	35.0	41.0	30.0	41.0
37	36.42728026489641	39.0	35.0	41.0	30.0	41.0
38	36.33649333212083	39.0	35.0	40.0	30.0	41.0
39	36.27457647065117	39.0	35.0	40.0	30.0	41.0
40	36.14594979164549	39.0	35.0	40.0	30.0	41.0
41	36.023802162179514	38.0	35.0	40.0	29.0	41.0
42	35.94170781155552	38.0	35.0	40.0	29.0	41.0
43	35.85319860276772	38.0	35.0	40.0	29.0	41.0
44	35.719301811801586	38.0	35.0	40.0	29.0	41.0
45	35.653282051556374	38.0	35.0	40.0	29.0	41.0
46	35.51874227697509	38.0	34.0	40.0	28.0	41.0
47	35.40864229890714	38.0	34.0	40.0	28.0	41.0
48	35.27601221775854	38.0	34.0	40.0	27.0	41.0
49	35.25381590983251	37.0	34.0	40.0	27.0	41.0
50	35.170430242696895	37.0	34.0	40.0	27.0	41.0
51	33.743886038910674	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	6.0
11	7.0
12	9.0
13	11.0
14	27.0
15	62.0
16	111.0
17	188.0
18	392.0
19	681.0
20	1054.0
21	1611.0
22	2456.0
23	3598.0
24	4825.0
25	6453.0
26	8061.0
27	9595.0
28	11313.0
29	13797.0
30	17315.0
31	22128.0
32	28462.0
33	39295.0
34	64283.0
35	80084.0
36	81507.0
37	115650.0
38	185369.0
39	236282.0
40	70.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.72738457588223	20.938156958612613	24.76300009093778	11.571458374567376
2	32.25199394461354	25.050042526786527	24.741068037509162	17.956895491090773
3	31.997368153588567	24.48301870643679	26.234587383185072	17.285025756789576
4	27.540132983133713	26.90517328996849	26.730679733177848	18.824013993719944
5	23.328964753585353	30.664113276381315	25.60176740254947	20.40515456748386
6	24.16869493583537	36.35542764829545	24.98125076895919	14.494626646909989
7	80.5717311879149	6.774222883155648	8.188465879609074	4.465580049320374
8	81.18176323011004	5.3884380633461895	8.081801210007436	5.34799749653634
9	74.8271379740132	7.992147255016288	10.620035198271111	6.560679572699407
10	38.333056953798256	25.125253422202725	19.259873436003875	17.28181618799514
11	29.74681851493252	26.256091494107764	22.419479942869675	21.57761004809004
12	25.67205695914754	22.73134304406203	31.21476829587945	20.381831700910983
13	24.391545995795465	25.03142702777882	30.945271502773604	19.631755473652117
14	20.151170690217768	28.715263104401924	29.57703232570704	21.556533879673264
15	20.108269453998854	26.220679251742528	32.723479600515674	20.947571693742944
16	21.289711727229445	24.453811630407454	30.975869391947192	23.28060725041591
17	21.24670350538405	24.230639613567917	27.942184967449624	26.58047191359841
18	22.003305855858265	25.455945993655753	29.943886038910673	22.596862111575312
19	23.14345167726716	27.271385089413236	26.833813877105612	22.751349356213993
20	23.85330130896914	26.32959061950027	27.309044029934576	22.50806404159601
21	23.36897737788928	26.879817696492474	28.506748118390295	21.244456807227948
22	22.123878656902445	25.216191204711645	26.086840233014698	26.573089905371212
23	21.238144655265565	27.331617997122088	26.995255187465567	24.434982160146784
24	21.980517917417792	25.856286207947964	28.488025633756102	23.67517024087814
25	21.6802092638854	26.833171963346725	27.081913544915242	24.404705227852638
26	20.443455421764085	26.599301383859082	27.997068593834417	24.960174600542416
27	23.181538560294424	26.13273706677508	27.612455266634928	23.07326910629557
28	20.30908147490385	26.721264998047513	29.357711791420822	23.611941735627816
29	21.222096811293405	25.711641640945537	28.9065534045501	24.15970814321096
30	23.498857928437314	26.00135871745631	28.120316035540625	22.37946731856575
31	23.620714556999268	28.61544551489507	24.85971509727668	22.90412483082898
32	22.3706944971943	26.995255187465567	26.940157589827805	23.693892725512328
33	22.072846513070967	25.901969070455383	26.668735055445303	25.356449361028343
34	19.966620484537902	27.12021439919547	29.04488581959014	23.86827929667649
35	21.287679000326307	25.097758116197088	28.395911009355896	25.218651874120713
36	23.36084647027672	26.725544423106758	26.68392701440562	23.22968209221091
37	21.487207193713527	28.10940350163956	26.949465339331663	23.45392396531526
38	20.991007858094264	27.721901562525076	27.373770333955633	23.913320245425027
39	21.42141103342766	25.41422159932813	28.66455191744989	24.499815449794323
40	22.596327183442906	23.99248960902103	30.062961041184117	23.348222166351952
41	19.867123851910495	25.289155401971747	27.087369811865774	27.756350934251984
42	21.452864807613096	25.319646305518855	27.62775421122172	25.599734675646328
43	22.313564172653404	23.732942479177925	28.751745203031977	25.201748145136698
44	21.50860431900974	24.142804414226948	28.88216068171241	25.466430585050897
45	22.55524470287417	24.17768172845978	28.19862951412478	25.068444054541274
46	23.677523924660722	24.77936889178939	28.111864171048616	23.43124301250127
47	21.005557903295692	25.42427824821735	29.45934813657785	24.110815711909105
48	20.861448264425675	24.830615006873828	28.454432147041047	25.853504581659454
49	21.63730802766648	23.45381697968878	29.836472469923663	25.07240252272107
50	20.69733231340369	23.58027399018942	30.187064367902174	25.535329328504712
51	20.467206230842887	23.041494375230688	28.09282072953499	28.39847866439144
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3737.0
1	3570.0
2	3403.0
3	3724.0
4	4045.0
5	3047.5
6	2050.0
7	2099.5
8	2149.0
9	2319.5
10	2490.0
11	2600.5
12	2711.0
13	2755.5
14	2800.0
15	2785.5
16	2771.0
17	2798.0
18	2825.0
19	2720.5
20	2616.0
21	2961.5
22	3307.0
23	3704.0
24	4101.0
25	4855.5
26	6185.0
27	6760.0
28	8956.5
29	11153.0
30	12571.5
31	13990.0
32	15107.0
33	16224.0
34	17464.5
35	18705.0
36	21552.0
37	24399.0
38	26708.0
39	29017.0
40	34375.0
41	39733.0
42	46977.0
43	54221.0
44	64526.0
45	74831.0
46	83961.0
47	93091.0
48	92509.0
49	91927.0
50	81967.5
51	72008.0
52	65618.0
53	59228.0
54	55038.0
55	50848.0
56	48236.5
57	45625.0
58	42688.5
59	39752.0
60	36176.0
61	32600.0
62	29789.0
63	26978.0
64	24548.5
65	22119.0
66	19922.5
67	17726.0
68	15595.5
69	13465.0
70	11921.5
71	10378.0
72	9920.5
73	9463.0
74	7886.5
75	5297.0
76	4284.0
77	3415.0
78	2546.0
79	1873.5
80	1201.0
81	1011.0
82	821.0
83	557.0
84	293.0
85	251.5
86	210.0
87	144.0
88	78.0
89	58.0
90	38.0
91	28.0
92	18.0
93	31.5
94	45.0
95	23.5
96	2.0
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	934705.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.94720408941707
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.72831186010468	19.59824662773013
2	11.360800215794944	6.1228360406823965
3	4.290458386327861	3.468475733205837
4	2.1551269370088626	2.3229858164071246
5	1.2684137115358092	1.709010157728522
6	0.8760933763337992	1.416496020807202
7	0.6186411973286073	1.1669455421774724
8	0.47788283153665556	1.030208495379742
9	0.40259852123998563	0.9764014066156266
>10	4.278665574847378	27.129499757923263
>50	1.3586304040784183	25.062406198756566
>100	0.17477818210008877	6.977161420752112
>500	0.00599925110183371	1.0786158283988867
>1k	0.0035995506611002257	1.9407109534351275
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3283	0.3512338117373931	No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	2675	0.28618655083689504	RNA PCR Primer, Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	2522	0.26981774998528946	TruSeq Adapter, Index 10 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	2400	0.25676550355459743	Illumina PCR Primer Index 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1660	0.17759613995859655	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCC	1494	0.1598365259627369	No Hit
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1459	0.15609202903589903	Illumina PCR Primer Index 2 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1394	0.1491379633146287	No Hit
ACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1120	0.11982390165881213	TruSeq Adapter, Index 10 (95% over 23bp)
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	961	0.10281318704832006	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0698562648108227E-4	0.0	0.0	0.27794865759785176	0.0
2	1.0698562648108227E-4	0.0	0.0	1.1278424743635693	0.0
3	1.0698562648108227E-4	0.0	0.0	1.5068925489860436	0.0
4	1.0698562648108227E-4	0.0	0.0	2.0199956135893142	0.0
5	1.0698562648108227E-4	0.0	0.0	3.5949310210173264	0.0
6	1.0698562648108227E-4	0.0	0.0	4.637933893581398	0.0
7	1.0698562648108227E-4	0.0	0.0	5.581547119144543	0.0
8	1.0698562648108227E-4	0.0	0.0	6.996325043730375	0.0
9	1.0698562648108227E-4	0.0	0.0	7.595337566397954	0.0
10	1.0698562648108227E-4	0.0	0.0	8.941537704409413	0.0
11	1.0698562648108227E-4	0.0	0.0	10.664220262007799	0.0
12	1.0698562648108227E-4	0.0	0.0	11.937242231506197	0.0
13	1.0698562648108227E-4	0.0	0.0	12.410867599937948	0.0
14	1.0698562648108227E-4	0.0	0.0	12.59402699247356	0.0
15	1.0698562648108227E-4	0.0	0.0	12.920761095746787	0.0
16	1.0698562648108227E-4	0.0	0.0	13.679289187497659	0.0
17	1.0698562648108227E-4	0.0	0.0	14.636382601997422	0.0
18	1.0698562648108227E-4	0.0	0.0	15.772676940852996	0.0
19	1.0698562648108227E-4	0.0	0.0	16.301506892548986	0.0
20	1.0698562648108227E-4	0.0	0.0	16.838146794978094	0.0
21	1.0698562648108227E-4	0.0	0.0	17.475674143178864	0.0
22	3.209568794432468E-4	0.0	0.0	18.15439095757485	0.0
23	3.209568794432468E-4	0.0	0.0	18.84241552147469	0.0
24	6.419137588864936E-4	0.0	0.0	19.389860972178386	0.0
25	6.419137588864936E-4	0.0	0.0	19.878892270823414	0.0
26	6.419137588864936E-4	0.0	0.0	20.348559171075365	0.0
27	6.419137588864936E-4	0.0	0.0	20.796935931657583	0.0
28	6.419137588864936E-4	0.0	0.0	21.24659651975757	0.0
29	6.419137588864936E-4	0.0	0.0	21.70331815920531	0.0
30	6.419137588864936E-4	0.0	0.0	22.242632702296447	0.0
31	6.419137588864936E-4	0.0	0.0	22.72449596396724	0.0
32	6.419137588864936E-4	0.0	0.0	23.188492626015694	0.0
33	6.419137588864936E-4	0.0	0.0	23.672281628963148	0.0
34	6.419137588864936E-4	0.0	0.0	24.112741453185766	0.0
35	6.419137588864936E-4	0.0	0.0	24.613648156370193	0.0
36	6.419137588864936E-4	0.0	0.0	25.022547220780886	0.0
37	6.419137588864936E-4	0.0	0.0	25.459155562450185	0.0
38	6.419137588864936E-4	0.0	0.0	25.87179912378772	0.0
39	6.419137588864936E-4	0.0	0.0	26.31364976115459	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	20	7.032608E-4	45.000004	1
CGAACTT	20	7.032608E-4	45.000004	6
CTATCGG	20	7.032608E-4	45.000004	2
GCACGAC	20	7.032608E-4	45.000004	22
CGATTGT	40	6.8121153E-9	45.000004	10
CGACATA	20	7.032608E-4	45.000004	25
ATCGTAG	20	7.032608E-4	45.000004	1
TCGTCGA	20	7.032608E-4	45.000004	42
ACAGCGA	20	7.032608E-4	45.000004	26
ACTCGAC	20	7.032608E-4	45.000004	35
CGTAACA	20	7.032608E-4	45.000004	17
ATCGGTT	20	7.032608E-4	45.000004	17
CGCGTAC	20	7.032608E-4	45.000004	43
CCGTAGT	35	1.2116107E-7	45.0	27
CGGTCTA	35	1.2116107E-7	45.0	31
CCGCAAT	25	3.8902457E-5	45.0	28
ACGACGT	25	3.8902457E-5	45.0	23
ACGATAG	30	2.1650649E-6	44.999996	1
CCGTACT	30	2.1650649E-6	44.999996	44
TACGCAT	110	0.0	42.954544	16
>>END_MODULE