FastQCFastQC Report
Sat 18 Jun 2016
SRR3553660_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553660_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587866
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31230.5312435146785152No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14240.24223207329561497No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13410.22811320947290709No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCC10780.18337512290215796No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC9700.16500358925333325No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC8690.14782280315582122No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT8110.13795660915923016No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC7610.1294512695069965No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC7560.12860073554177312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCGG850.045.0000042
TCTATAC302.1636843E-645.00000410
TCGCCAA207.030471E-445.027
TCGTTCA502.1827873E-1145.016
TACCCGA207.030471E-445.028
GTATTCG207.030471E-445.038
TTTACGT253.8884733E-545.027
ACGCATT1350.045.017
CGTGAGT207.030471E-445.032
TAAACGG551.8189894E-1245.02
CGTCTTG207.030471E-445.018
CGTTCAT502.1827873E-1145.017
GCACCGT207.030471E-445.024
ATCGTGA207.030471E-445.030
TACGCAT1350.045.016
CGTAGTG207.030471E-445.028
CGCTATA207.030471E-445.034
GATATCG207.030471E-445.012
TTGCGAA253.8884733E-545.014
TCCGTTA207.030471E-445.023