##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553660_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	587866
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.048669254558014	33.0	31.0	34.0	30.0	34.0
2	32.18871817727169	34.0	31.0	34.0	30.0	34.0
3	32.21757509364379	34.0	31.0	34.0	30.0	34.0
4	35.764728696675775	37.0	35.0	37.0	35.0	37.0
5	35.696857787318876	37.0	35.0	37.0	33.0	37.0
6	35.66750075697523	37.0	35.0	37.0	33.0	37.0
7	35.804608533237165	37.0	35.0	37.0	35.0	37.0
8	35.73063929534962	37.0	35.0	37.0	35.0	37.0
9	37.535957173913786	39.0	37.0	39.0	35.0	39.0
10	36.993658418755295	39.0	37.0	39.0	33.0	39.0
11	36.92192778626422	39.0	37.0	39.0	33.0	39.0
12	36.76770216341819	39.0	35.0	39.0	33.0	39.0
13	36.66015554565156	39.0	35.0	39.0	33.0	39.0
14	37.8137789904502	40.0	37.0	41.0	33.0	41.0
15	37.93437960351509	40.0	37.0	41.0	33.0	41.0
16	38.00332898993988	40.0	37.0	41.0	33.0	41.0
17	37.91991372183457	40.0	36.0	41.0	33.0	41.0
18	37.87146050290372	40.0	36.0	41.0	33.0	41.0
19	37.75405619648015	40.0	36.0	41.0	33.0	41.0
20	37.65779276229617	39.0	35.0	41.0	33.0	41.0
21	37.55120724791024	39.0	35.0	41.0	33.0	41.0
22	37.49404626224344	39.0	35.0	41.0	33.0	41.0
23	37.41140498004647	39.0	35.0	41.0	33.0	41.0
24	37.38078745836636	39.0	35.0	41.0	33.0	41.0
25	37.2667359568337	39.0	35.0	41.0	32.0	41.0
26	37.19883953145785	39.0	35.0	41.0	32.0	41.0
27	37.14085692998064	39.0	35.0	41.0	32.0	41.0
28	37.032876539891745	39.0	35.0	41.0	32.0	41.0
29	36.94668512892394	39.0	35.0	41.0	31.0	41.0
30	36.805023593812194	39.0	35.0	41.0	31.0	41.0
31	36.68411168531604	39.0	35.0	41.0	31.0	41.0
32	36.53219951485543	39.0	35.0	41.0	31.0	41.0
33	36.399657404918806	39.0	35.0	41.0	30.0	41.0
34	36.27775377381921	39.0	35.0	41.0	30.0	41.0
35	36.075250142039174	39.0	35.0	41.0	30.0	41.0
36	35.962550989511215	38.0	35.0	40.0	29.0	41.0
37	35.85680920481879	38.0	35.0	40.0	29.0	41.0
38	35.71052076493623	38.0	35.0	40.0	28.0	41.0
39	35.621104129172295	38.0	35.0	40.0	28.0	41.0
40	35.49122419054682	38.0	34.0	40.0	27.0	41.0
41	35.336956041002544	38.0	34.0	40.0	27.0	41.0
42	35.242921005807446	38.0	34.0	40.0	26.0	41.0
43	35.14314486634709	38.0	34.0	40.0	26.0	41.0
44	34.99290144352624	38.0	34.0	40.0	26.0	41.0
45	34.873440545974766	37.0	34.0	40.0	25.0	41.0
46	34.733631473839274	37.0	34.0	40.0	25.0	41.0
47	34.61129236934948	37.0	33.0	40.0	24.0	41.0
48	34.45738994941024	37.0	33.0	40.0	24.0	41.0
49	34.36529753379171	36.0	33.0	40.0	24.0	41.0
50	34.22805367209534	36.0	33.0	40.0	24.0	41.0
51	32.756005620328445	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	1.0
11	3.0
12	5.0
13	5.0
14	17.0
15	38.0
16	87.0
17	202.0
18	341.0
19	612.0
20	977.0
21	1598.0
22	2281.0
23	3129.0
24	4185.0
25	5295.0
26	6505.0
27	7603.0
28	8818.0
29	10022.0
30	12347.0
31	15104.0
32	19081.0
33	25681.0
34	43605.0
35	54772.0
36	49219.0
37	68964.0
38	108316.0
39	139006.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.01473465041353	21.057179697414036	23.993052838572055	11.935032813600378
2	31.40919869494068	25.327370523214476	25.199960535224015	18.063470246620827
3	31.544263488618157	24.928129879938627	27.022144502318557	16.505462129124666
4	26.40720844546206	27.816032905458048	26.714251206907697	19.062507442172198
5	24.11229770049637	32.54942452871913	25.211357690358007	18.126920080426494
6	24.42019099590723	37.476227575672006	24.380726219920867	13.722855208499896
7	80.26591093888743	7.741219937876999	7.857913197905646	4.134955925329923
8	82.49856259759876	6.231522149605522	7.083076755587157	4.186838497208548
9	76.86258433044266	8.682931144172311	9.20481878523337	5.249665740151667
10	40.33980532978604	26.802196418911794	16.981931256442795	15.876066994859372
11	31.312067716112175	27.70036028618767	21.27270500420164	19.714866993498518
12	27.33105843848768	23.88180299592084	29.15171144444482	19.635427121146655
13	24.107704817084166	26.88520853391759	29.460795487406994	19.54629116159125
14	21.11042312363702	30.00956000176911	28.446108466895513	20.433908407698354
15	20.568973201375822	26.415713785114292	32.21975756379855	20.79555544971133
16	22.506489574154653	25.07135979968224	31.07306767188441	21.349082954278696
17	22.212885249359548	24.350787424345004	27.741356023311436	25.694971302984015
18	23.417581557701926	25.900290202188934	28.866782566094994	21.815345674014146
19	25.423991181663848	27.475989426161746	25.687826817676136	21.41219257449827
20	24.764317038236605	26.504849744669706	26.388496698227147	22.342336518866546
21	23.843358860692742	28.181592403711047	26.855780058720864	21.11926867687534
22	22.41854436215056	25.751786971860934	25.46294563727108	26.36672302871743
23	21.557123562172332	28.63968999738036	25.856912969962544	23.94627347048477
24	22.14450231855559	26.802196418911794	28.25626928585766	22.797031976674955
25	22.780871831335713	27.001731687153196	26.54159281196735	23.67580366954374
26	20.948311349865445	28.6813661616763	25.74396205938088	24.626360429077373
27	22.7892070641949	26.69809106156845	26.63362058700452	23.879081287232122
28	21.450636709726368	28.22088707290437	27.75938734337417	22.569088873995096
29	23.077708185198667	26.85407899079042	26.965158726648593	23.103054097362325
30	22.853507431965788	26.97145267799124	26.87806404860972	23.29697584143325
31	25.8887229402619	27.12454879173145	23.637019320729554	23.3497089472771
32	24.99889430584521	27.587409375606004	24.530250091007133	22.88344622754165
33	23.720711862907535	27.012788628701102	23.445308964968206	25.82119054342316
34	22.60242980543185	27.122847723801	26.22672513804166	24.047997332725483
35	22.751443356138985	25.64257841072625	26.30973725304746	25.296240980087298
36	24.176768175060303	28.13022015221156	24.50133193618954	23.1916797365386
37	22.90641064460268	27.96028346595993	26.650291052722903	22.483014836714492
38	22.80298571443152	29.211759142389592	24.88356190016092	23.10169324301797
39	23.217876182667478	25.897058173121085	26.540572171209085	24.344493473002352
40	23.27026907492524	25.78955067991685	28.312064313976315	22.628115931181597
41	21.07333984275328	26.816485389527543	26.483756502332163	25.62641826538701
42	22.690034803849855	27.296526759499613	25.608216838531227	24.4052215981193
43	23.32895591852565	24.971847325751106	26.363661106442628	25.335535649280615
44	22.903518829120923	24.62363872038866	26.80253663249788	25.670305817992535
45	23.30207904522459	25.49458550077739	26.594155811018155	24.60917964297986
46	23.798960987708085	27.012618521908056	25.626928585766144	23.561491904617718
47	22.824759383941238	26.64944051875768	27.09869255918866	23.427107538112427
48	22.068124368478532	26.49736504577574	26.673765790163063	24.760744795582667
49	22.551227660725402	25.743621845794788	27.609523258701813	24.095627234777993
50	22.054685931828004	25.20642459335971	28.0218621250421	24.717027349770184
51	22.42551874066539	25.39303174532972	26.038757131727298	26.142692382277595
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3506.0
1	3238.5
2	2971.0
3	3407.5
4	3844.0
5	2874.0
6	1904.0
7	1967.0
8	2030.0
9	2180.0
10	2330.0
11	2427.5
12	2525.0
13	2473.0
14	2421.0
15	2404.5
16	2388.0
17	2392.0
18	2396.0
19	2288.5
20	2181.0
21	2141.5
22	2102.0
23	2151.5
24	2201.0
25	2828.5
26	4127.0
27	4798.0
28	5603.0
29	6408.0
30	7865.0
31	9322.0
32	9958.0
33	10594.0
34	11976.0
35	13358.0
36	14191.5
37	15025.0
38	16811.0
39	18597.0
40	19938.5
41	21280.0
42	24908.0
43	28536.0
44	33023.5
45	37511.0
46	41333.0
47	45155.0
48	47569.5
49	49984.0
50	48697.0
51	47410.0
52	43465.0
53	39520.0
54	36191.5
55	32863.0
56	30631.5
57	28400.0
58	27887.5
59	27375.0
60	25205.0
61	23035.0
62	21832.5
63	20630.0
64	18936.5
65	17243.0
66	15281.5
67	13320.0
68	11675.5
69	10031.0
70	9372.0
71	8713.0
72	7993.5
73	7274.0
74	6658.0
75	4928.0
76	3814.0
77	2956.5
78	2099.0
79	1876.5
80	1654.0
81	1178.0
82	702.0
83	573.0
84	444.0
85	366.0
86	288.0
87	189.0
88	90.0
89	75.0
90	60.0
91	40.5
92	21.0
93	13.0
94	5.0
95	3.5
96	2.0
97	2.0
98	2.0
99	4.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	587866.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.191838727418187
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.62049163269707	20.83452051691793
2	9.227690972703677	5.018357695124221
3	3.2078875311319215	2.616850812067047
4	1.640506562678004	1.7843355953444575
5	1.0466425054794484	1.423006710712903
6	0.7046238225645987	1.1496010408004096
7	0.522741343733053	0.9950008820460137
8	0.4536341573602176	0.9868117478549826
9	0.3526112488162461	0.8629333390156403
>10	4.6939598496711215	31.4090951999671
>50	1.3841394917678862	25.210841897385833
>100	0.13816274418735705	5.615368714856795
>500	0.00439608731505227	0.903704991607279
>1k	0.0025120498943155827	1.1895708562993776
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3123	0.5312435146785152	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1424	0.24223207329561497	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1341	0.22811320947290709	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCC	1078	0.18337512290215796	No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	970	0.16500358925333325	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	869	0.14782280315582122	No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT	811	0.13795660915923016	No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	761	0.1294512695069965	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	756	0.12860073554177312	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7010679304467344E-4	0.0	0.0	0.14305981295057038	0.0
2	1.7010679304467344E-4	0.0	0.0	0.6040492221016354	0.0
3	1.7010679304467344E-4	0.0	0.0	0.8399873440545975	0.0
4	1.7010679304467344E-4	0.0	0.0	1.150262134568082	0.0
5	1.7010679304467344E-4	0.0	0.0	2.0082807986854148	0.0
6	1.7010679304467344E-4	0.0	0.0	2.602974147169593	0.0
7	1.7010679304467344E-4	0.0	0.0	3.161434748735256	0.0
8	1.7010679304467344E-4	0.0	0.0	4.084774421381743	0.0
9	1.7010679304467344E-4	0.0	0.0	4.4952421129985405	0.0
10	1.7010679304467344E-4	0.0	0.0	5.419942639989386	0.0
11	1.7010679304467344E-4	0.0	0.0	6.48838340710298	0.0
12	1.7010679304467344E-4	0.0	0.0	7.250631946736161	0.0
13	1.7010679304467344E-4	0.0	0.0	7.553592145148724	0.0
14	1.7010679304467344E-4	0.0	0.0	7.674538075003487	0.0
15	1.7010679304467344E-4	0.0	0.0	7.867098964730057	0.0
16	1.7010679304467344E-4	0.0	0.0	8.355135353975225	0.0
17	1.7010679304467344E-4	0.0	0.0	8.934519091085383	0.0
18	1.7010679304467344E-4	0.0	0.0	9.652029544147815	0.0
19	1.7010679304467344E-4	0.0	0.0	9.976933518863142	0.0
20	1.7010679304467344E-4	0.0	0.0	10.311193367195926	0.0
21	1.7010679304467344E-4	0.0	0.0	10.701078136854317	0.0
22	1.7010679304467344E-4	0.0	0.0	11.108994226575444	0.0
23	1.7010679304467344E-4	0.0	0.0	11.527116723879251	0.0
24	1.7010679304467344E-4	0.0	0.0	11.870902552622537	0.0
25	3.402135860893469E-4	0.0	0.0	12.182538197480378	0.0
26	3.402135860893469E-4	0.0	0.0	12.480225085308557	0.0
27	3.402135860893469E-4	0.0	0.0	12.778422293515868	0.0
28	3.402135860893469E-4	0.0	0.0	13.10349637502424	0.0
29	3.402135860893469E-4	0.0	0.0	13.42346725274127	0.0
30	3.402135860893469E-4	0.0	0.0	13.784263760789024	0.0
31	3.402135860893469E-4	0.0	0.0	14.112059550986109	0.0
32	3.402135860893469E-4	0.0	0.0	14.454314418591991	0.0
33	3.402135860893469E-4	0.0	0.0	14.797249713370054	0.0
34	3.402135860893469E-4	0.0	0.0	15.126746571497586	0.0
35	5.103203791340204E-4	0.0	0.0	15.485331691235757	0.0
36	5.103203791340204E-4	0.0	0.0	15.811766627088486	0.0
37	5.103203791340204E-4	0.0	0.0	16.117958854568897	0.0
38	5.103203791340204E-4	0.0	0.0	16.469569595792237	0.0
39	6.804271721786938E-4	0.0	0.0	16.796684958817146	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGCGG	85	0.0	45.000004	2
TCTATAC	30	2.1636843E-6	45.000004	10
TCGCCAA	20	7.030471E-4	45.0	27
TCGTTCA	50	2.1827873E-11	45.0	16
TACCCGA	20	7.030471E-4	45.0	28
GTATTCG	20	7.030471E-4	45.0	38
TTTACGT	25	3.8884733E-5	45.0	27
ACGCATT	135	0.0	45.0	17
CGTGAGT	20	7.030471E-4	45.0	32
TAAACGG	55	1.8189894E-12	45.0	2
CGTCTTG	20	7.030471E-4	45.0	18
CGTTCAT	50	2.1827873E-11	45.0	17
GCACCGT	20	7.030471E-4	45.0	24
ATCGTGA	20	7.030471E-4	45.0	30
TACGCAT	135	0.0	45.0	16
CGTAGTG	20	7.030471E-4	45.0	28
CGCTATA	20	7.030471E-4	45.0	34
GATATCG	20	7.030471E-4	45.0	12
TTGCGAA	25	3.8884733E-5	45.0	14
TCCGTTA	20	7.030471E-4	45.0	23
>>END_MODULE