##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553659_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1492351
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23618371281287	33.0	31.0	34.0	30.0	34.0
2	32.41755324317135	34.0	31.0	34.0	31.0	34.0
3	32.3884796539152	34.0	31.0	34.0	30.0	34.0
4	35.94181663697079	37.0	35.0	37.0	35.0	37.0
5	35.881612971747266	37.0	35.0	37.0	35.0	37.0
6	35.85625298606025	37.0	35.0	37.0	35.0	37.0
7	36.00970348128557	37.0	35.0	37.0	35.0	37.0
8	35.96333034252666	37.0	35.0	37.0	35.0	37.0
9	37.75646479950092	39.0	38.0	39.0	35.0	39.0
10	37.23258603371459	39.0	37.0	39.0	34.0	39.0
11	37.15377548579389	39.0	37.0	39.0	34.0	39.0
12	37.092064802449286	39.0	37.0	39.0	33.0	39.0
13	37.10988768728	39.0	37.0	39.0	33.0	39.0
14	38.34790273869887	40.0	38.0	41.0	34.0	41.0
15	38.426780294984226	40.0	38.0	41.0	34.0	41.0
16	38.470363205438936	40.0	38.0	41.0	34.0	41.0
17	38.419722974018846	40.0	38.0	41.0	34.0	41.0
18	38.260639085577054	40.0	37.0	41.0	34.0	41.0
19	38.078257058828655	40.0	37.0	41.0	34.0	41.0
20	37.92127589286971	40.0	36.0	41.0	34.0	41.0
21	37.826628588046646	40.0	35.0	41.0	33.0	41.0
22	37.782553166111725	40.0	35.0	41.0	33.0	41.0
23	37.70054229869515	40.0	35.0	41.0	33.0	41.0
24	37.663190496069625	40.0	35.0	41.0	33.0	41.0
25	37.58257474280514	39.0	35.0	41.0	33.0	41.0
26	37.51878344973803	39.0	35.0	41.0	33.0	41.0
27	37.44519218334025	39.0	35.0	41.0	33.0	41.0
28	37.331950057325656	39.0	35.0	41.0	33.0	41.0
29	37.21262558205141	39.0	35.0	41.0	33.0	41.0
30	37.00205045595842	39.0	35.0	41.0	32.0	41.0
31	36.77654385596954	39.0	35.0	41.0	31.0	41.0
32	36.44785174533337	39.0	35.0	41.0	30.0	41.0
33	36.15698987704635	39.0	35.0	41.0	29.0	41.0
34	35.90618091856406	39.0	35.0	41.0	27.0	41.0
35	35.678570255925045	39.0	35.0	41.0	26.0	41.0
36	35.557223468205535	39.0	35.0	41.0	25.0	41.0
37	35.459440842000305	39.0	35.0	41.0	24.0	41.0
38	35.342054248631854	39.0	35.0	41.0	24.0	41.0
39	35.25292240230348	39.0	35.0	41.0	23.0	41.0
40	35.13917905372128	39.0	35.0	41.0	23.0	41.0
41	34.98179248715617	38.0	34.0	41.0	23.0	41.0
42	34.8687895810034	38.0	34.0	40.0	23.0	41.0
43	34.77555012192172	38.0	34.0	40.0	22.0	41.0
44	34.63477626912167	38.0	34.0	40.0	22.0	41.0
45	34.546948405569466	38.0	34.0	40.0	22.0	41.0
46	34.42350626628722	38.0	34.0	40.0	21.0	41.0
47	34.33025943628543	38.0	34.0	40.0	20.0	41.0
48	34.17768943097167	37.0	33.0	40.0	20.0	41.0
49	34.109588829973646	37.0	33.0	40.0	20.0	41.0
50	34.00551679866198	37.0	33.0	40.0	20.0	41.0
51	32.715911337212226	36.0	31.0	39.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	0.0
9	6.0
10	4.0
11	15.0
12	17.0
13	26.0
14	39.0
15	99.0
16	178.0
17	333.0
18	671.0
19	1277.0
20	2273.0
21	3511.0
22	5407.0
23	8468.0
24	13374.0
25	20759.0
26	27476.0
27	28193.0
28	26685.0
29	25934.0
30	28539.0
31	32676.0
32	40162.0
33	53534.0
34	86322.0
35	107896.0
36	116130.0
37	163783.0
38	275608.0
39	422787.0
40	167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.08983007348807	18.26688225491188	22.652043654609404	16.991244016990642
2	36.82873533103137	22.068534815200984	24.034694250883337	17.068035602884308
3	31.0679592133486	22.547309580654954	31.125988457139105	15.25874274885734
4	25.879769571635624	25.977199733842777	30.45543575204493	17.68759494247667
5	22.924432656928566	30.258297143232387	29.369498194459613	17.44777200537943
6	23.531260407236633	35.25819328026717	28.19705283810578	13.013493474390408
7	79.52445503772236	5.609404221929023	11.736247035717469	3.129893704631149
8	81.88201033134966	4.151972290701048	11.199979093390228	2.7660382845590616
9	76.1897837707081	7.002039064536427	12.984947911047737	3.8232292537077406
10	36.60532944327441	27.584730401896067	21.53434413217802	14.275596022651508
11	28.069535920168914	26.493164141679802	27.774900140784574	17.662399797366703
12	23.277566738655985	24.463681801399268	33.19125326414497	19.067498195799782
13	23.12284442466953	24.778219065085892	33.98034376631235	18.118592743932226
14	21.51564879843951	26.87156037688185	32.75931734558425	18.853473479094397
15	21.128206434009158	25.79922551732133	33.873465424688966	19.19910262398055
16	23.722100229771684	24.73660687063566	32.953641603081316	18.587651296511343
17	24.27558932181504	24.62992955410624	31.412315199306327	19.68216592477239
18	25.169614923030842	23.73979043804038	32.08012056144969	19.010474077479092
19	23.835880432954447	25.69656870267115	30.544623885399613	19.922926978974786
20	24.822042535569715	25.16043477707322	30.826595083864316	19.190927603492742
21	24.080863014130056	25.85819287821699	31.770943966935395	18.290000140717567
22	23.61636103034742	23.75587244555738	31.54653295370861	21.081233570386594
23	21.715400733473558	26.566940351164035	31.34383265062978	20.373826264732628
24	20.990571253009513	25.78408162690949	32.89581338438477	20.329533735696227
25	22.26922486734019	26.739754923607112	31.007919718618478	19.98310049043422
26	22.18593347007507	26.13319520675766	30.74544795426813	20.93542336889914
27	22.079390170274955	25.79158656375075	31.555512074572267	20.573511191402023
28	21.145293566995967	26.574378279640648	32.307613959450556	19.97271419391283
29	22.07925615354565	25.945638794090662	31.532930255683816	20.44217479667987
30	22.284770807939953	25.508074172899004	32.08769250665561	20.119462512505436
31	23.033656291314845	26.54060606385495	29.965135547870442	20.46060209695976
32	22.11711587957525	27.11500176567041	30.356665422544697	20.411216932209648
33	22.469177827468204	26.24690840157577	29.155875527942154	22.128038243013876
34	21.89230281616054	27.397509031052348	30.035896380945232	20.67429177184188
35	22.057344418303735	27.468470889221102	29.24874912135282	21.225435571122343
36	23.266778391946666	28.06886583652237	27.956626825726655	20.707728945804305
37	22.08073033756804	28.43962311815384	28.231763170996636	21.247883373281486
38	22.803817600551078	28.485121797754015	27.860469822447936	20.850590779246975
39	23.17792530041525	27.14709877233975	27.600142325766523	22.07483360147847
40	23.728264999319865	27.09637344029655	28.420860776050677	20.754500784332908
41	22.124687824781166	27.247410294227027	28.466694497474123	22.161207383517684
42	22.446327975121132	27.429002962439803	28.42541734484716	21.69925171759191
43	22.311775178895584	26.457917741871718	29.379013382240505	21.851293696992197
44	22.635291563445865	26.984737504782725	28.393253329813163	21.986717601958254
45	22.867073496784602	26.653984216849793	28.555078530452953	21.923863755912652
46	22.458590505852847	26.852060942767487	28.42642247031697	22.262926081062698
47	21.55350852446911	28.15517261019693	28.943928070541048	21.347390794792915
48	21.7667961491633	27.687923283463473	28.719450048949614	21.825830518423615
49	22.730912499807353	27.05482825421097	28.811854583807694	21.40240466217398
50	21.584265363845372	27.034658736450073	28.946139346574633	22.434936553129926
51	21.769476483749468	26.67408672624604	28.017470420832634	23.538966369171863
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2858.0
1	2934.0
2	3010.0
3	14242.0
4	25474.0
5	17714.0
6	9954.0
7	10124.5
8	10295.0
9	10639.5
10	10984.0
11	11055.0
12	11126.0
13	10936.0
14	10746.0
15	10509.0
16	10272.0
17	9893.0
18	9514.0
19	9450.5
20	9387.0
21	9573.0
22	9759.0
23	10283.0
24	10807.0
25	11693.5
26	14348.5
27	16117.0
28	18759.0
29	21401.0
30	23407.5
31	25414.0
32	30589.5
33	35765.0
34	38658.0
35	41551.0
36	46524.0
37	51497.0
38	56351.0
39	61205.0
40	66032.0
41	70859.0
42	75119.5
43	79380.0
44	87030.5
45	94681.0
46	99915.0
47	105149.0
48	105311.5
49	105474.0
50	101494.0
51	97514.0
52	95406.5
53	93299.0
54	86437.0
55	79575.0
56	76922.0
57	74269.0
58	68533.5
59	62798.0
60	58218.5
61	53639.0
62	48558.0
63	43477.0
64	38666.0
65	33855.0
66	30116.5
67	26378.0
68	23022.0
69	19666.0
70	17305.0
71	14944.0
72	13736.0
73	12528.0
74	10944.0
75	7646.5
76	5933.0
77	5215.0
78	4497.0
79	3508.0
80	2519.0
81	2061.5
82	1604.0
83	1088.5
84	573.0
85	408.0
86	243.0
87	190.0
88	137.0
89	135.0
90	133.0
91	94.5
92	56.0
93	51.5
94	47.0
95	35.5
96	24.0
97	24.0
98	24.0
99	12.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1492351.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.585705270206912
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.06925656383655	20.48940540400118
2	7.498536156921769	3.987077444518243
3	2.89637683925451	2.310066629996216
4	1.6453287239445447	1.7496889810958114
5	1.0565665873636436	1.4044783944999075
6	0.8137343436678437	1.2980220857399132
7	0.6450066624791589	1.2003569918193524
8	0.5512426454020493	1.1724139602422448
9	0.4547236995678712	1.0880235230480582
>10	6.004833848835333	37.82983428614878
>50	1.2795992318591598	22.49376715489742
>100	0.08047879916574174	3.1397237941167617
>500	0.003554268695565405	0.6044160618737197
>1k	5.077526707950578E-4	0.24001155957821851
>5k	0.0	0.0
>10k+	2.538763353975289E-4	0.9927137284241951
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14708	0.985559027333382	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2411	0.16155716718117924	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.700836465415978E-5	0.0	0.0	0.04462757085967041	0.0
2	6.700836465415978E-5	0.0	0.0	0.13549091333071106	0.0
3	6.700836465415978E-5	0.0	0.0	0.22883356529395565	0.0
4	6.700836465415978E-5	0.0	0.0	0.32666577768902894	0.0
5	6.700836465415978E-5	0.0	0.0	0.5640094052940628	0.0
6	6.700836465415978E-5	0.0	0.0	0.8471867543225421	0.0
7	6.700836465415978E-5	0.0	0.0	1.0814479971534847	0.0
8	1.3401672930831956E-4	0.0	0.0	1.509430422199603	0.0
9	1.3401672930831956E-4	0.0	0.0	1.7309600757462553	0.0
10	1.3401672930831956E-4	0.0	0.0	2.1407162256064423	0.0
11	1.3401672930831956E-4	0.0	0.0	2.614867413899277	0.0
12	1.3401672930831956E-4	0.0	0.0	3.013701200320836	0.0
13	1.3401672930831956E-4	0.0	0.0	3.2056131566903496	0.0
14	2.0102509396247934E-4	0.0	0.0	3.300564009405294	0.0
15	2.0102509396247934E-4	0.0	0.0	3.4164214718923365	0.0
16	2.0102509396247934E-4	0.0	0.0	3.641368552036351	0.0
17	2.0102509396247934E-4	0.0	0.0	3.915767805295135	0.0
18	2.0102509396247934E-4	0.0	0.0	4.212011785431176	0.0
19	2.0102509396247934E-4	0.0	0.0	4.388444809565578	0.0
20	2.0102509396247934E-4	0.0	0.0	4.561527415467273	0.0
21	2.0102509396247934E-4	0.0	0.0	4.779840667510525	0.0
22	2.0102509396247934E-4	0.0	0.0	5.018792495867259	0.0
23	2.680334586166391E-4	0.0	0.0	5.2692027545798545	0.0
24	2.680334586166391E-4	0.0	0.0	5.471635024200071	0.0
25	2.680334586166391E-4	0.0	0.0	5.665021164591976	0.0
26	2.680334586166391E-4	0.0	0.0	5.839175904328138	0.0
27	2.680334586166391E-4	0.0	0.0	6.032562044720042	0.0
28	2.680334586166391E-4	0.0	0.0	6.241427117347058	0.0
29	2.680334586166391E-4	0.0	0.0	6.451297315443887	0.0
30	2.680334586166391E-4	0.0	0.0	6.708743452445169	0.0
31	2.680334586166391E-4	0.0	0.0	6.932283356931446	0.0
32	2.680334586166391E-4	0.0	0.0	7.174719620250196	0.0
33	2.680334586166391E-4	0.0	0.0	7.4048933528372345	0.0
34	2.680334586166391E-4	0.0	0.0	7.637412378187169	0.0
35	3.3504182327079887E-4	0.0	0.0	7.878441465848182	0.0
36	3.3504182327079887E-4	0.0	0.0	8.108079131517988	0.0
37	4.020501879249587E-4	0.0	0.0	8.34475267547648	0.0
38	4.020501879249587E-4	0.0	0.0	8.584374587479756	0.0
39	4.020501879249587E-4	0.0	0.0	8.854284280306711	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCCG	20	7.0339645E-4	45.0	1
ACGATAG	20	7.0339645E-4	45.0	1
TTGCGAT	20	7.0339645E-4	45.0	23
TCGTACC	25	3.891367E-5	45.0	36
CGTTCGA	30	2.1659398E-6	44.999996	14
CGTTTTT	11125	0.0	42.89663	1
CGCATCG	60	3.6379788E-12	41.249996	21
CGTATGG	205	0.0	40.609753	2
CGTTAGG	250	0.0	39.6	2
TAGTACG	40	3.459154E-7	39.375	1
ACACGAC	455	0.0	39.065933	26
TAACGCC	70	0.0	38.571426	12
ACTCGCA	35	6.2495437E-6	38.571426	37
TCGACGG	100	0.0	38.25	2
TCGTTTA	65	9.094947E-12	38.07692	38
AACACGT	480	0.0	37.968746	41
ACGACGA	30	1.1400854E-4	37.499996	17
GACACGA	475	0.0	37.42105	25
CACGACC	475	0.0	37.42105	27
CACAACG	345	0.0	37.173912	12
>>END_MODULE