SRR3553656_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553656_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	541757
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCG	2745	0.5066847313463416	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGC	1926	0.3555099426495643	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTC	1671	0.30844086924580577	RNA PCR Primer, Index 30 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1524	0.28130693281305086	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1387	0.25601884239612965	No Hit
GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	883	0.1629882031981128	Illumina Single End Adapter 2 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	720	0.13290091314002406	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCC	632	0.11665746820068777	No Hit
CCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	592	0.1092740841373531	RNA PCR Primer, Index 30 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTACGG	30	2.1633696E-6	45.000004	2
CATCGAA	30	2.1633696E-6	45.000004	42
TCGACAG	30	2.1633696E-6	45.000004	1
ACGTTAG	25	3.8880666E-5	45.0	1
CGACCGA	20	7.02998E-4	45.0	18
TATGCTA	20	7.02998E-4	45.0	38
GTAGCGT	20	7.02998E-4	45.0	42
GTATGCG	25	3.8880666E-5	45.0	1
GCGATTC	20	7.02998E-4	45.0	9
GCGATAA	25	3.8880666E-5	45.0	9
ACGTACG	20	7.02998E-4	45.0	1
CGCACAG	25	3.8880666E-5	45.0	2
ATCGGTT	25	3.8880666E-5	45.0	34
ATCGCAT	20	7.02998E-4	45.0	1
ATCGATC	20	7.02998E-4	45.0	25
GGCGATT	235	0.0	42.12766	8
TACGCAT	70	0.0	41.785713	16
AGTACGG	65	0.0	41.53846	2
CACCAAA	150	0.0	40.5	16
GCATATC	150	0.0	40.5	28