##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553656_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	541757
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.958080836980418	33.0	31.0	34.0	30.0	34.0
2	32.04572714335025	33.0	31.0	34.0	30.0	34.0
3	32.03729901044195	33.0	31.0	34.0	30.0	34.0
4	35.71662756549523	37.0	35.0	37.0	33.0	37.0
5	35.613561061509124	37.0	35.0	37.0	33.0	37.0
6	35.58830619632049	37.0	35.0	37.0	33.0	37.0
7	35.86674283857892	37.0	35.0	37.0	35.0	37.0
8	35.74769315394171	37.0	35.0	37.0	35.0	37.0
9	37.509267069922494	39.0	37.0	39.0	35.0	39.0
10	36.98498588850721	39.0	37.0	39.0	33.0	39.0
11	36.88649523679435	39.0	37.0	39.0	33.0	39.0
12	36.700162619033996	39.0	35.0	39.0	33.0	39.0
13	36.59414276142255	39.0	35.0	39.0	32.0	39.0
14	37.697008068192936	40.0	36.0	41.0	33.0	41.0
15	37.84350548308559	40.0	36.0	41.0	33.0	41.0
16	37.922420937800524	40.0	36.0	41.0	33.0	41.0
17	37.82356850026857	40.0	36.0	41.0	33.0	41.0
18	37.82034380727891	40.0	36.0	41.0	33.0	41.0
19	37.74031899910845	40.0	36.0	41.0	33.0	41.0
20	37.58952260884492	39.0	35.0	41.0	33.0	41.0
21	37.47410001162883	39.0	35.0	41.0	33.0	41.0
22	37.46869352864845	39.0	35.0	41.0	33.0	41.0
23	37.34210356303657	39.0	35.0	41.0	32.0	41.0
24	37.291462777592166	39.0	35.0	41.0	32.0	41.0
25	37.175842674852376	39.0	35.0	41.0	32.0	41.0
26	37.087915799888144	39.0	35.0	41.0	32.0	41.0
27	37.0457806728847	39.0	35.0	41.0	32.0	41.0
28	36.93540277282988	39.0	35.0	41.0	31.0	41.0
29	36.9098507264327	39.0	35.0	41.0	31.0	41.0
30	36.74631246112187	39.0	35.0	41.0	31.0	41.0
31	36.674049435448	39.0	35.0	41.0	31.0	41.0
32	36.47001515439579	39.0	35.0	41.0	30.0	41.0
33	36.346365621487124	39.0	35.0	41.0	30.0	41.0
34	36.117824042882695	39.0	35.0	41.0	30.0	41.0
35	36.037498361811664	38.0	35.0	41.0	30.0	41.0
36	35.87880728813841	38.0	35.0	40.0	29.0	41.0
37	35.77163193092106	38.0	35.0	40.0	29.0	41.0
38	35.69007507055746	38.0	35.0	40.0	28.0	41.0
39	35.530134359131495	38.0	35.0	40.0	27.0	41.0
40	35.411933763661565	38.0	34.0	40.0	27.0	41.0
41	35.25338482013154	38.0	34.0	40.0	26.0	41.0
42	35.15399524140897	38.0	34.0	40.0	26.0	41.0
43	35.089455235465344	38.0	34.0	40.0	26.0	41.0
44	35.00806080955115	37.0	34.0	40.0	26.0	41.0
45	34.94710543657027	37.0	34.0	40.0	26.0	41.0
46	34.75491594940167	37.0	34.0	40.0	25.0	41.0
47	34.598530337402195	37.0	34.0	40.0	24.0	41.0
48	34.54196992378502	37.0	34.0	40.0	24.0	41.0
49	34.479829517661976	36.0	33.0	40.0	24.0	41.0
50	34.35468300363447	36.0	33.0	40.0	24.0	41.0
51	32.80070400567044	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	6.0
11	7.0
12	5.0
13	9.0
14	21.0
15	37.0
16	75.0
17	157.0
18	275.0
19	539.0
20	988.0
21	1689.0
22	2501.0
23	3182.0
24	3966.0
25	4973.0
26	6042.0
27	6835.0
28	7748.0
29	9130.0
30	11152.0
31	13915.0
32	17770.0
33	24450.0
34	41192.0
35	51149.0
36	46729.0
37	64377.0
38	96438.0
39	126365.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.72959463375646	19.09380035698662	22.60367655609434	16.57292845316258
2	28.335397604461043	27.67089303876092	27.13707437098182	16.856634985796216
3	30.602650265709535	26.792639504427264	27.01838647216372	15.586323757699486
4	27.57250944611699	26.1718814893024	27.837757518592284	18.41785154598833
5	26.25863625204658	30.122545716991194	24.165262285489618	19.4535557454726
6	24.1266841037587	37.68405392085381	23.229233770860365	14.960028204527124
7	80.89789333594213	6.136330495037443	9.359177638683027	3.6065985303374024
8	82.99163647170225	7.1862477088436325	6.633232242499866	3.188883576954243
9	78.56308271051412	6.881313947027912	7.663768073139803	6.891835269318163
10	45.40744282030504	23.56148605371043	15.333627438131856	15.697443687852672
11	34.888889299076894	26.441928761418865	19.935690724808357	18.73349121469589
12	29.163074957960855	22.053983612579074	27.039244532142643	21.743696897317434
13	24.989801700762516	25.836860437428587	28.801658308060624	20.37167955374827
14	21.279466624335264	28.609136568609173	27.80840856694053	22.30298824011503
15	20.195032090032985	24.832166451010323	32.439636220667204	22.53316523828949
16	22.570082158606162	22.673264950891266	30.52789350206827	24.228759388434298
17	22.64003972260626	24.236142772497633	27.66480174690867	25.459015757987437
18	23.048340861308667	23.818981572919224	28.979782448588576	24.152895117183533
19	24.71347855219222	25.94428867555011	25.274061987200902	24.06817078505677
20	26.84801488490227	24.86096164885733	26.357758183096852	21.933265283143548
21	24.954915211063263	26.87330297531919	26.124812415898642	22.046969397718904
22	24.875728416983996	23.628305679483606	25.91475513929677	25.581210764235625
23	24.429772019558584	26.719728586801832	25.367092626398918	23.483406767240663
24	23.24529263119812	25.435388928984764	28.722840683184526	22.596477756632584
25	22.827393093213377	27.244502609103343	27.17362212209533	22.75448217558795
26	21.979411433539394	26.900252327150366	27.077268960068814	24.04306727924143
27	23.177550082417024	26.787840304786094	26.753876738094757	23.280732874702124
28	21.21412367537475	27.19983313552017	29.094409486171845	22.491633702933235
29	22.203497139861597	25.13562353601338	28.848727381464386	23.812151942660638
30	22.459331397656147	27.262038146253765	26.959134815055457	23.319495641034635
31	24.460043894218256	27.292494605515017	23.852575970407397	24.394885529859327
32	26.0897413415978	27.37851102985287	24.075000415315355	22.456747213233978
33	25.40677831573934	26.143086291455393	23.296422565836714	25.15371282696855
34	22.85729579866988	27.750633586644934	24.606234898672284	24.7858357160129
35	24.420911958682584	25.850888867148925	25.411208346177343	24.316990827991148
36	25.08523193978112	27.515288219626143	23.889862059927236	23.5096177806655
37	23.918841842375826	27.26960611491868	26.064822420384047	22.746729622321446
38	24.49326912250326	26.845799869683272	24.896032723158168	23.7648982846553
39	24.450076325732756	26.838231901018357	24.870560048139666	23.84113172510923
40	24.23595818789605	26.518162201872798	26.23870111507558	23.00717849515558
41	21.323766928715273	27.03112280967297	25.77557834970291	25.869531911908844
42	21.966305926826973	25.743460629027403	26.6501401920049	25.640093252140723
43	22.963062775377153	24.231528157458047	26.11262983219414	26.692779234970658
44	23.359735084179807	25.355832965702334	26.248299514357914	25.036132435759946
45	23.675928506692117	25.083755262968456	25.97954433445253	25.2607718958869
46	24.476656508360758	27.27551282216935	25.510884031032365	22.736946638437527
47	22.616782062806756	26.468139774843703	27.977672646592477	22.937405515757064
48	23.75419237776346	26.147885491096563	25.977144734631946	24.12077739650803
49	22.690615903440104	25.24212885112698	27.526917049525895	24.540338195907022
50	23.4164025568659	25.077848555717786	26.92258706394195	24.583161823474363
51	22.220294338605683	26.05337817508588	25.57641156459446	26.14991592171398
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1958.0
1	2098.5
2	2239.0
3	2597.0
4	2955.0
5	2180.0
6	1405.0
7	1443.5
8	1482.0
9	1541.5
10	1601.0
11	1597.0
12	1593.0
13	1644.0
14	1695.0
15	1745.5
16	1796.0
17	1720.0
18	1644.0
19	1585.5
20	1527.0
21	1717.5
22	1908.0
23	2129.5
24	2351.0
25	2728.0
26	3413.5
27	3722.0
28	4749.5
29	5777.0
30	7325.0
31	8873.0
32	9408.5
33	9944.0
34	11753.0
35	13562.0
36	14547.0
37	15532.0
38	15756.0
39	15980.0
40	17984.0
41	19988.0
42	22626.0
43	25264.0
44	28419.0
45	31574.0
46	37575.5
47	43577.0
48	41400.5
49	39224.0
50	39259.5
51	39295.0
52	36873.0
53	34451.0
54	32572.5
55	30694.0
56	29411.0
57	28128.0
58	27225.5
59	26323.0
60	25579.0
61	24835.0
62	23388.0
63	21941.0
64	19823.0
65	17705.0
66	16222.0
67	14739.0
68	12973.0
69	11207.0
70	10586.0
71	9965.0
72	8372.0
73	6779.0
74	6187.0
75	4900.5
76	4206.0
77	3240.5
78	2275.0
79	1922.5
80	1570.0
81	1201.0
82	832.0
83	611.0
84	390.0
85	332.5
86	275.0
87	228.0
88	181.0
89	121.0
90	61.0
91	41.0
92	21.0
93	13.0
94	5.0
95	6.0
96	7.0
97	4.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	541757.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.376859920163298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.9829348506882	18.5222535917812
2	8.067989984729946	3.9334452339008465
3	2.7351298424788077	2.0002163110069264
4	1.4573312188540526	1.4210063591714432
5	0.9765458906001356	1.190256119038531
6	0.7088254204287475	1.036736278898546
7	0.5740272294647376	0.9795086954114941
8	0.4858645043281097	0.9475080769748724
9	0.43649568349587303	0.9576354719101349
>10	7.348900564338949	46.22098369516456
>50	1.1561171371716936	17.762010215530978
>100	0.061487516816091395	2.5982308587232694
>500	0.004554630875266029	0.7179784724625229
>1k	0.003795525729388358	1.712230620024671
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCG	2745	0.5066847313463416	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGC	1926	0.3555099426495643	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTC	1671	0.30844086924580577	RNA PCR Primer, Index 30 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1524	0.28130693281305086	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1387	0.25601884239612965	No Hit
GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	883	0.1629882031981128	Illumina Single End Adapter 2 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	720	0.13290091314002406	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCC	632	0.11665746820068777	No Hit
CCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	592	0.1092740841373531	RNA PCR Primer, Index 30 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07697177886026392	0.0
2	0.0	0.0	0.0	0.4330354753145783	0.0
3	0.0	0.0	0.0	0.5334494985759298	0.0
4	0.0	0.0	0.0	0.7161882541434629	0.0
5	0.0	0.0	0.0	1.4517578914531792	0.0
6	0.0	0.0	0.0	1.8510143846780014	0.0
7	0.0	0.0	0.0	2.2497171240980736	0.0
8	0.0	0.0	0.0	2.7218845349483254	0.0
9	1.845846015833667E-4	0.0	0.0	2.9066537211332757	0.0
10	1.845846015833667E-4	0.0	0.0	3.869262418390533	0.0
11	1.845846015833667E-4	0.0	0.0	4.4846674800694775	0.0
12	3.691692031667334E-4	0.0	0.0	5.338740431595715	0.0
13	3.691692031667334E-4	0.0	0.0	5.5310775864455834	0.0
14	3.691692031667334E-4	0.0	0.0	5.6220777950261835	0.0
15	3.691692031667334E-4	0.0	0.0	5.833980917643888	0.0
16	3.691692031667334E-4	0.0	0.0	6.164018185274948	0.0
17	3.691692031667334E-4	0.0	0.0	6.537063665074932	0.0
18	3.691692031667334E-4	0.0	0.0	6.984127570109846	0.0
19	3.691692031667334E-4	0.0	0.0	7.335022897719827	0.0
20	3.691692031667334E-4	0.0	0.0	7.569445341730702	0.0
21	3.691692031667334E-4	0.0	0.0	7.8596123354197545	0.0
22	3.691692031667334E-4	0.0	0.0	8.176544096338395	0.0
23	3.691692031667334E-4	0.0	0.0	8.489968749826952	0.0
24	3.691692031667334E-4	0.0	0.0	8.729374978080578	0.0
25	5.537538047501001E-4	0.0	0.0	8.935740562650782	0.0
26	5.537538047501001E-4	0.0	0.0	9.136014855368735	0.0
27	5.537538047501001E-4	0.0	0.0	9.339796255516772	0.0
28	5.537538047501001E-4	0.0	0.0	9.550776455126561	0.0
29	7.383384063334668E-4	0.0	0.0	9.757695793501515	0.0
30	7.383384063334668E-4	0.0	0.0	10.000793713786809	0.0
31	7.383384063334668E-4	0.0	0.0	10.2261715123201	0.0
32	7.383384063334668E-4	0.0	0.0	10.450810972447057	0.0
33	7.383384063334668E-4	0.0	0.0	10.663452433471095	0.0
34	7.383384063334668E-4	0.0	0.0	10.877385986706217	0.0
35	7.383384063334668E-4	0.0	0.0	11.139496120954597	0.0
36	7.383384063334668E-4	0.0	0.0	11.37650274938764	0.0
37	7.383384063334668E-4	0.0	0.0	11.614063131625434	0.0
38	7.383384063334668E-4	0.0	0.0	11.825781669641556	0.0
39	7.383384063334668E-4	0.0	0.0	12.048390699151096	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTACGG	30	2.1633696E-6	45.000004	2
CATCGAA	30	2.1633696E-6	45.000004	42
TCGACAG	30	2.1633696E-6	45.000004	1
ACGTTAG	25	3.8880666E-5	45.0	1
CGACCGA	20	7.02998E-4	45.0	18
TATGCTA	20	7.02998E-4	45.0	38
GTAGCGT	20	7.02998E-4	45.0	42
GTATGCG	25	3.8880666E-5	45.0	1
GCGATTC	20	7.02998E-4	45.0	9
GCGATAA	25	3.8880666E-5	45.0	9
ACGTACG	20	7.02998E-4	45.0	1
CGCACAG	25	3.8880666E-5	45.0	2
ATCGGTT	25	3.8880666E-5	45.0	34
ATCGCAT	20	7.02998E-4	45.0	1
ATCGATC	20	7.02998E-4	45.0	25
GGCGATT	235	0.0	42.12766	8
TACGCAT	70	0.0	41.785713	16
AGTACGG	65	0.0	41.53846	2
CACCAAA	150	0.0	40.5	16
GCATATC	150	0.0	40.5	28
>>END_MODULE