##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553653_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 458138 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05151504568492 33.0 31.0 34.0 30.0 34.0 2 32.134819639497266 33.0 31.0 34.0 30.0 34.0 3 32.132322575293905 33.0 31.0 34.0 30.0 34.0 4 35.78653375183897 37.0 35.0 37.0 35.0 37.0 5 35.68717504332756 37.0 35.0 37.0 33.0 37.0 6 35.66929396819299 37.0 35.0 37.0 33.0 37.0 7 35.910550969358574 37.0 35.0 37.0 35.0 37.0 8 35.824463371298606 37.0 35.0 37.0 35.0 37.0 9 37.57220095255141 39.0 37.0 39.0 35.0 39.0 10 37.0643452409536 39.0 37.0 39.0 33.0 39.0 11 37.00140568998861 39.0 37.0 39.0 33.0 39.0 12 36.828307191282974 39.0 35.0 39.0 33.0 39.0 13 36.77384325246978 39.0 35.0 39.0 33.0 39.0 14 37.89150212381422 40.0 37.0 41.0 33.0 41.0 15 38.04576132082473 40.0 37.0 41.0 33.0 41.0 16 38.074176339880125 40.0 37.0 41.0 33.0 41.0 17 38.02478292566869 40.0 37.0 41.0 33.0 41.0 18 38.00211071773134 40.0 36.0 41.0 33.0 41.0 19 37.914778953066545 40.0 36.0 41.0 33.0 41.0 20 37.85542784051967 40.0 36.0 41.0 33.0 41.0 21 37.68638488839607 40.0 35.0 41.0 33.0 41.0 22 37.67895262999358 40.0 35.0 41.0 33.0 41.0 23 37.600454448223026 40.0 35.0 41.0 33.0 41.0 24 37.549011433236274 40.0 35.0 41.0 33.0 41.0 25 37.42679280042258 39.0 35.0 41.0 33.0 41.0 26 37.37132698008024 39.0 35.0 41.0 33.0 41.0 27 37.33577219091191 39.0 35.0 41.0 33.0 41.0 28 37.22348069795564 39.0 35.0 41.0 32.0 41.0 29 37.19730954428578 39.0 35.0 41.0 32.0 41.0 30 37.037015047867676 39.0 35.0 41.0 32.0 41.0 31 36.935270595322805 39.0 35.0 41.0 31.0 41.0 32 36.778863573857656 39.0 35.0 41.0 31.0 41.0 33 36.69978696375328 39.0 35.0 41.0 31.0 41.0 34 36.557279684287266 39.0 35.0 41.0 31.0 41.0 35 36.450237701303976 39.0 35.0 41.0 30.0 41.0 36 36.308105854567835 39.0 35.0 41.0 30.0 41.0 37 36.236740894664926 39.0 35.0 41.0 30.0 41.0 38 36.12503874378462 38.0 35.0 41.0 30.0 41.0 39 36.05934238155316 38.0 35.0 41.0 30.0 41.0 40 35.9045025734604 38.0 35.0 40.0 29.0 41.0 41 35.77013258013961 38.0 35.0 40.0 29.0 41.0 42 35.60981407348877 38.0 35.0 40.0 28.0 41.0 43 35.530073907861826 38.0 35.0 40.0 28.0 41.0 44 35.43322972554121 38.0 34.0 40.0 27.0 41.0 45 35.363645888356785 38.0 34.0 40.0 27.0 41.0 46 35.205490485399594 37.0 34.0 40.0 27.0 41.0 47 35.12632001711275 37.0 34.0 40.0 26.0 41.0 48 35.03091644875561 37.0 34.0 40.0 26.0 41.0 49 34.91667139595493 37.0 34.0 40.0 26.0 41.0 50 34.81236876225068 37.0 34.0 40.0 26.0 41.0 51 33.373858968258475 35.0 32.0 39.0 22.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 3.0 10 6.0 11 6.0 12 5.0 13 12.0 14 14.0 15 31.0 16 66.0 17 130.0 18 224.0 19 396.0 20 736.0 21 1108.0 22 1578.0 23 2147.0 24 2800.0 25 3504.0 26 4217.0 27 5043.0 28 5973.0 29 7266.0 30 8732.0 31 10947.0 32 14005.0 33 19223.0 34 32290.0 35 41881.0 36 37550.0 37 53053.0 38 84237.0 39 120903.0 40 49.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.006526417804245 18.0921469077003 22.738126939917667 14.163199734577791 2 29.796917086118157 25.874736433127136 26.50795175252871 17.820394728225995 3 31.919639933819067 25.598837031636755 25.93672648852529 16.544796546018887 4 27.56702129052818 27.680087659176927 25.248287633857046 19.504603416437842 5 24.71787976548551 31.33881057672579 24.099507135404615 19.843802522384085 6 25.10750036015349 36.49926441377925 23.709886540736633 14.683348685330621 7 82.30838742911524 6.862779337230267 7.08672932609825 3.742103907556239 8 84.12705342058506 6.3275694223138 6.136578934731456 3.408798222369679 9 79.04888919932422 8.295971956048177 7.52480693590141 5.130331908726192 10 43.222566126363674 25.52484185987628 15.698326705053937 15.55426530870611 11 33.90703237889021 27.331939284669687 20.42572325369212 18.335305082747993 12 28.53921744103305 24.80759072593847 27.47796515460407 19.175226678424405 13 24.590188982359027 26.917871907591163 27.705407540959275 20.786531569090535 14 20.84830334964574 29.385032457469144 28.020814689023833 21.74584950386128 15 21.283761661333482 27.034867223413034 30.199852446206165 21.48151866904732 16 23.657500578428333 24.19445669208841 29.924171319558734 22.22387140992452 17 22.895284826842566 24.891844815317654 26.854790477978252 25.358079879861528 18 24.86827113227892 24.926550515346904 27.38672627025045 22.818452082123727 19 25.08087082931344 27.225857710995378 25.501049901994595 22.19222155769659 20 27.61613313019221 25.640964076326345 24.22436034557273 22.51854244790871 21 26.06376244712292 28.079530621777714 24.542386791752705 21.31432013934666 22 24.340700836865746 25.57941057061409 25.286704006216464 24.793184586303692 23 23.74742981372425 27.386289720564545 25.233444944536366 23.63283552117484 24 22.755152377667866 27.262091334925287 28.3993032667013 21.58345302070555 25 24.13006561341779 27.53886383578747 26.28793071083385 22.043139839960883 26 21.727514417053374 28.757928833670203 25.6935683134776 23.82098843579882 27 23.39164181971371 26.631713588482075 27.497828165312637 22.478816426491584 28 21.260842802823603 28.25786116846889 27.9627535807988 22.51854244790871 29 22.55019230013664 27.846631364348735 26.440723100899728 23.162453234614897 30 23.82273463454243 27.312949373332923 25.8601120186494 23.00420397347524 31 25.39867900065046 27.82873282722673 24.29049762298696 22.48209054913585 32 25.588359839175094 28.385333676752424 24.584077286756393 21.44222919731609 33 25.41308514028524 27.909712793961646 22.651908376951923 24.02529368880119 34 23.467819739903696 27.906438671317375 24.994433991504746 23.631307597274184 35 23.970506703220426 26.713348379745838 25.146789831884714 24.169355085149018 36 24.572945269765878 29.412753362523958 24.669641025193283 21.34466034251688 37 23.78781065967023 28.946300023137134 25.277754737655467 21.98813457953717 38 23.533520467632023 29.503773972034626 24.855611191387748 22.107094368945603 39 25.263130323177734 27.451990448292875 24.641265295609617 22.643613932919777 40 24.95885519210369 26.957379654165337 25.80881743055586 22.274947723175114 41 22.154896559551926 26.536327482112377 25.330140699963767 25.97863525837193 42 22.961422104256794 27.2830457198486 25.28081058545678 24.474721590437813 43 23.533738742474974 25.878010555771404 25.781969624872854 24.806281076880765 44 23.35300717251134 26.654850721834904 26.118985982389585 23.87315612326417 45 23.452758775740058 27.733346720857032 24.971733407837814 23.842161095565093 46 24.596737227647562 27.847286188877586 25.165561468378524 22.390415115096324 47 23.135605428931893 27.531442491127127 27.226075985838328 22.106876094102653 48 23.17882384783624 26.834927467269686 26.467134356896832 23.51911432799724 49 22.998092277872605 27.648437806949 26.50511417957035 22.84835573560805 50 22.38277549559303 26.55313464501962 27.169542801513952 23.894547057873393 51 22.59493864294165 26.285966237247294 26.22463100637799 24.89446411343307 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1703.0 1 1904.5 2 2106.0 3 2185.0 4 2264.0 5 1662.0 6 1060.0 7 1072.0 8 1084.0 9 1202.0 10 1320.0 11 1292.5 12 1265.0 13 1200.0 14 1135.0 15 1148.5 16 1162.0 17 1249.5 18 1337.0 19 1447.5 20 1558.0 21 1419.5 22 1281.0 23 1724.0 24 2167.0 25 2408.0 26 2852.0 27 3055.0 28 4825.0 29 6595.0 30 7366.5 31 8138.0 32 8621.0 33 9104.0 34 10565.5 35 12027.0 36 12690.5 37 13354.0 38 13788.0 39 14222.0 40 16260.5 41 18299.0 42 20937.0 43 23575.0 44 25932.0 45 28289.0 46 30288.0 47 32287.0 48 32395.5 49 32504.0 50 32966.0 51 33428.0 52 31981.5 53 30535.0 54 29338.5 55 28142.0 56 26732.5 57 25323.0 58 23484.5 59 21646.0 60 21175.0 61 20704.0 62 19011.5 63 17319.0 64 16127.0 65 14935.0 66 12999.5 67 11064.0 68 9717.5 69 8371.0 70 7941.5 71 7512.0 72 6552.5 73 5593.0 74 4959.5 75 3254.0 76 2182.0 77 1892.0 78 1602.0 79 1287.0 80 972.0 81 696.0 82 420.0 83 365.5 84 311.0 85 206.0 86 101.0 87 81.5 88 62.0 89 43.0 90 24.0 91 16.0 92 8.0 93 8.5 94 9.0 95 7.0 96 5.0 97 2.5 98 0.0 99 2.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 458138.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.002552014712126 #Duplication Level Percentage of deduplicated Percentage of total 1 75.76964113255971 16.671254701552165 2 7.922209454833854 3.486176512028521 3 2.6558321972585914 1.7530525818758806 4 1.4468247152696554 1.273353442155666 5 0.9448833781494616 1.0394922837785219 6 0.7559066351440991 0.9979125034814437 7 0.6629181181864641 1.0210123263824928 8 0.4929391134557177 0.8676734787116409 9 0.46194294201890057 0.9147531248640005 >10 6.7561654961494115 37.38541901725105 >50 1.973756206807597 28.624864860155956 >100 0.1519812276771807 4.969542649723035 >500 0.0039995059915047555 0.7116946510183726 >1k 9.998764978761889E-4 0.28379786702123666 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1290 0.28157454740711313 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 989 0.21587381967878677 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 977 0.21325452156337174 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 752 0.16414268189934036 No Hit ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC 517 0.11284809380579651 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.3654968590250097E-4 0.0 0.0 0.0657007277283264 0.0 2 4.3654968590250097E-4 0.0 0.0 0.2964172367277982 0.0 3 4.3654968590250097E-4 0.0 0.0 0.3747779053472971 0.0 4 4.3654968590250097E-4 0.0 0.0 0.48085947902160486 0.0 5 4.3654968590250097E-4 0.0 0.0 0.8202768598107993 0.0 6 4.3654968590250097E-4 0.0 0.0 1.0861356185254225 0.0 7 4.3654968590250097E-4 0.0 0.0 1.306156660220283 0.0 8 4.3654968590250097E-4 0.0 0.0 1.7112747687378038 0.0 9 4.3654968590250097E-4 0.0 0.0 1.8703971292492656 0.0 10 4.3654968590250097E-4 0.0 0.0 2.3217895044724517 0.0 11 4.3654968590250097E-4 0.0 0.0 2.8078875797248863 0.0 12 4.3654968590250097E-4 0.0 0.0 3.2363610964381913 0.0 13 4.3654968590250097E-4 0.0 0.0 3.381950416686675 0.0 14 4.3654968590250097E-4 0.0 0.0 3.4371739519533415 0.0 15 4.3654968590250097E-4 0.0 0.0 3.5541692677752117 0.0 16 4.3654968590250097E-4 0.0 0.0 3.7988553667235636 0.0 17 4.3654968590250097E-4 0.0 0.0 4.097018802194972 0.0 18 4.3654968590250097E-4 0.0 0.0 4.449096123875339 0.0 19 4.3654968590250097E-4 0.0 0.0 4.64663485674622 0.0 20 4.3654968590250097E-4 0.0 0.0 4.809467889587854 0.0 21 4.3654968590250097E-4 0.0 0.0 5.0067883476157835 0.0 22 4.3654968590250097E-4 0.0 0.0 5.237286581772304 0.0 23 4.3654968590250097E-4 0.0 0.0 5.453378676294043 0.0 24 4.3654968590250097E-4 0.0 0.0 5.651353958850827 0.0 25 4.3654968590250097E-4 0.0 0.0 5.810258044519337 0.0 26 4.3654968590250097E-4 0.0 0.0 5.958030113197333 0.0 27 4.3654968590250097E-4 0.0 0.0 6.113441801378624 0.0 28 4.3654968590250097E-4 0.0 0.0 6.2795489568645255 0.0 29 4.3654968590250097E-4 0.0 0.0 6.442818539392061 0.0 30 4.3654968590250097E-4 0.0 0.0 6.647996891766236 0.0 31 4.3654968590250097E-4 0.0 0.0 6.834840157332507 0.0 32 4.3654968590250097E-4 0.0 0.0 7.011861054965971 0.0 33 4.3654968590250097E-4 0.0 0.0 7.194775373359119 0.0 34 4.3654968590250097E-4 0.0 0.0 7.389039983585731 0.0 35 4.3654968590250097E-4 0.0 0.0 7.591817312687444 0.0 36 4.3654968590250097E-4 0.0 0.0 7.786955022285862 0.0 37 4.3654968590250097E-4 0.0 0.0 7.979473433768864 0.0 38 4.3654968590250097E-4 0.0 0.0 8.190326932059772 0.0 39 4.3654968590250097E-4 0.0 0.0 8.401616980036582 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACACT 65 0.0 45.000004 18 ACGTTAG 30 2.1626329E-6 45.000004 1 ACGCTAG 30 2.1626329E-6 45.000004 1 AGGTATG 20 7.0288405E-4 45.0 1 CGGCGAA 20 7.0288405E-4 45.0 31 CGAACCC 20 7.0288405E-4 45.0 34 ACGTTAT 20 7.0288405E-4 45.0 10 GCGAACC 20 7.0288405E-4 45.0 33 GTTTACG 20 7.0288405E-4 45.0 1 CGTTGTA 25 3.88712E-5 45.0 10 CTATACG 20 7.0288405E-4 45.0 1 GGCCGTA 40 6.7993824E-9 45.0 42 TCCTCGA 20 7.0288405E-4 45.0 21 TTACTAA 20 7.0288405E-4 45.0 41 CGATTCG 25 3.88712E-5 45.0 10 TTATCGG 40 6.7993824E-9 45.0 2 ACGGGTC 25 3.88712E-5 45.0 5 CGTTATA 20 7.0288405E-4 45.0 11 CCCGTTT 25 3.88712E-5 45.0 45 ATTCCCG 25 3.88712E-5 45.0 42 >>END_MODULE