FastQCFastQC Report
Sat 18 Jun 2016
SRR3553652_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553652_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580807
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19490.3355675809692376No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11390.19610645188504958No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11370.19576210341817504No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA9730.1675255291344629No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT9290.15994986286322307No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC7720.1329185082135718No Hit
GTGGGGAGGGAGTGTTCTGCTGGTCTCCAATTACCAAGAATTCTCCAAAAA6050.10416541122954787No Hit
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC5870.101066275027677TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCC5830.10037757809392793No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGTA207.0304004E-445.00000439
CGACGGT207.0304004E-445.00000428
ATCGTAG207.0304004E-445.0000041
ACGATTG207.0304004E-445.00000413
CCCGTTG207.0304004E-445.0000049
AAGCACG207.0304004E-445.00000425
TATTCGA207.0304004E-445.00000427
CGAATCA207.0304004E-445.00000443
ATGGTCG207.0304004E-445.00000444
TAGGCAC207.0304004E-445.00000410
TTCGATC207.0304004E-445.00000419
GCACTAG406.8048394E-945.0000041
TGCACGA207.0304004E-445.00000439
ACGAATC207.0304004E-445.00000442
GCGAATG351.2105374E-745.01
GACGTCT253.8884144E-545.020
CCAATGT253.8884144E-545.015
CGTTCCA253.8884144E-545.028
TATTGCG253.8884144E-545.01
ACGATAG253.8884144E-545.01