##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553652_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	580807
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0124723014702	33.0	31.0	34.0	30.0	34.0
2	32.0935990785235	33.0	31.0	34.0	30.0	34.0
3	32.091021630248946	33.0	31.0	34.0	30.0	34.0
4	35.761359625486605	37.0	35.0	37.0	35.0	37.0
5	35.65738188417151	37.0	35.0	37.0	33.0	37.0
6	35.643019109618166	37.0	35.0	37.0	33.0	37.0
7	35.87829003438319	37.0	35.0	37.0	35.0	37.0
8	35.81580111809947	37.0	35.0	37.0	35.0	37.0
9	37.54783947163171	39.0	37.0	39.0	35.0	39.0
10	37.03853431518559	39.0	37.0	39.0	33.0	39.0
11	36.98552703393727	39.0	37.0	39.0	33.0	39.0
12	36.84024469402056	39.0	35.0	39.0	33.0	39.0
13	36.799942149457564	39.0	35.0	39.0	33.0	39.0
14	37.88498416857924	40.0	37.0	41.0	33.0	41.0
15	38.062166950467194	40.0	37.0	41.0	33.0	41.0
16	38.07963058296474	40.0	37.0	41.0	33.0	41.0
17	38.04254253134002	40.0	37.0	41.0	33.0	41.0
18	37.992677429851916	40.0	37.0	41.0	33.0	41.0
19	37.89667652077196	40.0	36.0	41.0	33.0	41.0
20	37.86944200052685	40.0	36.0	41.0	33.0	41.0
21	37.699139301007044	40.0	36.0	41.0	33.0	41.0
22	37.68869693374908	40.0	35.0	41.0	33.0	41.0
23	37.598166000065426	39.0	35.0	41.0	33.0	41.0
24	37.53110069265694	39.0	35.0	41.0	33.0	41.0
25	37.34609775708626	39.0	35.0	41.0	32.0	41.0
26	37.31268734708776	39.0	35.0	41.0	32.0	41.0
27	37.295172062320184	39.0	35.0	41.0	32.0	41.0
28	37.201170096090436	39.0	35.0	41.0	32.0	41.0
29	37.146370481072026	39.0	35.0	41.0	32.0	41.0
30	37.01028396696321	39.0	35.0	41.0	31.0	41.0
31	36.94425687018235	39.0	35.0	41.0	31.0	41.0
32	36.7800629124649	39.0	35.0	41.0	31.0	41.0
33	36.67546706565176	39.0	35.0	41.0	31.0	41.0
34	36.552699950241646	39.0	35.0	41.0	30.0	41.0
35	36.44775975496163	39.0	35.0	41.0	30.0	41.0
36	36.28256374320557	39.0	35.0	41.0	30.0	41.0
37	36.261572260664906	39.0	35.0	41.0	30.0	41.0
38	36.12175128743283	39.0	35.0	41.0	30.0	41.0
39	36.0306452918095	38.0	35.0	40.0	29.0	41.0
40	35.85811810119368	38.0	35.0	40.0	29.0	41.0
41	35.702391672276676	38.0	35.0	40.0	28.0	41.0
42	35.60444519435888	38.0	35.0	40.0	28.0	41.0
43	35.518172129468134	38.0	34.0	40.0	28.0	41.0
44	35.369897401374295	38.0	34.0	40.0	27.0	41.0
45	35.31671966763486	38.0	34.0	40.0	27.0	41.0
46	35.115818163348585	37.0	34.0	40.0	26.0	41.0
47	35.02715532009773	37.0	34.0	40.0	26.0	41.0
48	34.94382299111409	37.0	34.0	40.0	26.0	41.0
49	34.859893217540424	37.0	34.0	40.0	26.0	41.0
50	34.69410492642134	37.0	34.0	40.0	26.0	41.0
51	33.14844690232728	35.0	31.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	1.0
10	3.0
11	7.0
12	6.0
13	9.0
14	18.0
15	37.0
16	93.0
17	137.0
18	264.0
19	513.0
20	831.0
21	1262.0
22	1789.0
23	2761.0
24	3578.0
25	4679.0
26	5749.0
27	6561.0
28	7852.0
29	9208.0
30	11191.0
31	14035.0
32	18147.0
33	24125.0
34	41590.0
35	52393.0
36	48557.0
37	68633.0
38	108198.0
39	148527.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.61740302716738	18.60256505172975	23.020728055963513	13.759303865139366
2	30.568157752919646	25.47851523828053	25.513638781901733	18.43968822689809
3	31.512189074856188	25.023458739305827	26.632254776543675	16.83209740929431
4	27.259829857422517	27.999490364269025	25.35747675217413	19.38320302613433
5	24.833722734057957	30.414578336693598	24.720604262689672	20.031094666558772
6	24.365925341808897	37.20323618689169	23.769341622948758	14.661496848350659
7	81.09303090355317	8.011267081836134	7.057077480126789	3.8386245344839165
8	83.69682183582499	6.088080894341838	6.849951877301755	3.365145392531426
9	78.96237476476695	8.3247963609254	7.922597351615941	4.790231522691703
10	42.59280621617852	26.703707083420134	15.24964402977237	15.453842670628969
11	32.894231646657154	27.373292677257677	20.97994686703156	18.752528809053608
12	28.37500236739571	25.233166955632424	27.164100983631396	19.22772969334047
13	25.352828047871323	25.953716122567393	28.14480541729008	20.5486504122712
14	21.967193921560863	29.1967899835918	27.809237836320843	21.026778258526498
15	20.96927206455845	26.719891461363243	31.10379179314299	21.20704468093532
16	22.80275547643193	23.986625505546595	31.573827450426734	21.63679156759474
17	22.837706845819696	24.53844392371304	27.261723773990326	25.362125456476935
18	24.45528376896284	24.90672460903536	27.797702162680547	22.840289459321255
19	25.352655873637886	26.94492318446575	25.876582065987495	21.825838875908865
20	27.556830410101806	25.689773022708064	24.968190810372466	21.785205756817668
21	26.381569092659007	26.353676866842168	25.61539375386316	21.649360286635662
22	24.629524093201354	25.335266276060725	24.422915013076633	25.61229461766129
23	23.1731022525555	28.66700986730532	26.14138603701402	22.01850184312517
24	23.522443772199715	26.754326308050697	27.645672314555437	22.077557605194155
25	23.6004386999468	27.197330610684787	26.584734688114985	22.61749600125343
26	21.754042220565523	28.540461805728924	26.03601540615041	23.669480567555144
27	23.474923683771028	27.401701425774828	27.377252684626736	21.746122205827408
28	21.588066259531995	27.859340538251086	28.339706649541068	22.212886552675844
29	22.02349489589485	27.302529067314964	27.634480989382016	23.039495047408177
30	23.702882368841973	26.664451358196438	26.335942920798132	23.296723352163458
31	24.619537987661992	27.75586382395529	24.4112071652029	23.213391023179817
32	24.88950718569163	28.247249086185256	25.403791620968757	21.459452107154355
33	25.192533836541227	27.29168209060841	23.591657814041497	23.924126258808865
34	23.03674025967318	28.49156432343274	25.260542658748946	23.211152758145133
35	23.727847632690377	26.71799754479543	25.025869178573952	24.528285643940244
36	24.332179192055193	28.940078201536828	24.260210362478414	22.46753224392957
37	24.02898036697216	28.945415602773384	25.199765154345595	21.825838875908865
38	23.476301077638528	28.70471602442808	24.99298389998743	22.825998997945963
39	24.769501744985856	26.11469903083124	25.385368977990968	23.730430246191936
40	24.999526520858048	26.475059701415443	25.887256868460607	22.6381569092659
41	21.90125119015439	27.084728662016815	25.610572875326916	25.403447272501882
42	23.39520701368957	27.95334766970784	24.59767186001546	24.053773456587127
43	23.57065255756215	26.18029741377084	25.377448963252853	24.871601065414158
44	22.68240568725928	26.78566201853628	26.564762477036265	23.967169817168184
45	24.2536677416078	26.289111529303195	25.688223454607122	23.768997274481883
46	23.025032411799444	27.32060736182587	25.84920636287097	23.805153863503712
47	22.59132551777096	27.35555873121364	26.899124838371442	23.15399091264396
48	23.03157503267006	27.16117402166296	26.41703698474708	23.390213960919894
49	23.50832548505786	26.43545962772487	26.836969940100587	23.219244947116685
50	22.091675892336006	27.173054043770133	27.089549540553058	23.6457205233408
51	22.142295116966565	27.454214566973196	25.138471127241925	25.265019188818314
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2358.0
1	2678.0
2	2998.0
3	2956.5
4	2915.0
5	2185.0
6	1455.0
7	1438.0
8	1421.0
9	1485.5
10	1550.0
11	1553.5
12	1557.0
13	1604.0
14	1651.0
15	1693.5
16	1736.0
17	1843.0
18	1950.0
19	1909.0
20	1868.0
21	1999.5
22	2131.0
23	2534.5
24	2938.0
25	4218.5
26	5179.0
27	4859.0
28	5638.0
29	6417.0
30	8353.0
31	10289.0
32	10877.5
33	11466.0
34	13105.0
35	14744.0
36	14976.5
37	15209.0
38	16113.5
39	17018.0
40	20687.0
41	24356.0
42	26422.0
43	28488.0
44	30711.0
45	32934.0
46	36781.0
47	40628.0
48	42550.5
49	44473.0
50	43025.0
51	41577.0
52	40050.0
53	38523.0
54	36304.5
55	34086.0
56	34032.0
57	33978.0
58	31226.0
59	28474.0
60	27523.0
61	26572.0
62	25413.5
63	24255.0
64	21842.5
65	19430.0
66	16807.0
67	14184.0
68	12243.0
69	10302.0
70	9120.0
71	7938.0
72	7605.5
73	7273.0
74	6100.0
75	3941.0
76	2955.0
77	2210.0
78	1465.0
79	1233.5
80	1002.0
81	744.5
82	487.0
83	365.0
84	243.0
85	185.5
86	128.0
87	77.5
88	27.0
89	28.0
90	29.0
91	21.0
92	13.0
93	12.0
94	11.0
95	11.5
96	12.0
97	9.5
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	580807.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.871145306482553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.71358037818176	17.545274438099238
2	8.430729669539911	3.85640886623442
3	2.8403955501684814	1.9488929806736939
4	1.4569417845945156	1.3328770903418867
5	0.9228336217246171	1.0553130928085634
6	0.6590782895711258	0.9044325197477523
7	0.5266463968257273	0.8431504386855677
8	0.41339418266675204	0.7563838736500702
9	0.37263711371809427	0.7670373816991318
>10	5.474009659044189	31.312662589080148
>50	1.9370604936209976	30.348102606323085
>100	0.24514978988583502	7.7333214756869255
>500	0.005280149320617985	0.8672513063462762
>1k	0.0022629211374077074	0.7288913406232379
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1949	0.3355675809692376	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1139	0.19610645188504958	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1137	0.19576210341817504	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	973	0.1675255291344629	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	929	0.15994986286322307	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	772	0.1329185082135718	No Hit
GTGGGGAGGGAGTGTTCTGCTGGTCTCCAATTACCAAGAATTCTCCAAAAA	605	0.10416541122954787	No Hit
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	587	0.101066275027677	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCC	583	0.10037757809392793	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08488189708457371	0.0
2	0.0	0.0	0.0	0.3520963073792155	0.0
3	0.0	0.0	0.0	0.45385127934064157	0.0
4	0.0	0.0	0.0	0.5917628403238941	0.0
5	0.0	0.0	0.0	0.9426539280690488	0.0
6	0.0	0.0	0.0	1.231906640243661	0.0
7	0.0	0.0	0.0	1.4834531952955112	0.0
8	0.0	0.0	0.0	1.9006313629140144	0.0
9	0.0	0.0	0.0	2.1037969583699923	0.0
10	0.0	0.0	0.0	2.5325107996287923	0.0
11	0.0	0.0	0.0	3.195037249895404	0.0
12	0.0	0.0	0.0	3.6805685881885033	0.0
13	0.0	0.0	0.0	3.867721979934815	0.0
14	0.0	0.0	0.0	4.007527457485877	0.0
15	0.0	0.0	0.0	4.144750321535381	0.0
16	0.0	0.0	0.0	4.488065743009296	0.0
17	0.0	0.0	0.0	4.8508368528616215	0.0
18	0.0	0.0	0.0	5.299006382498834	0.0
19	0.0	0.0	0.0	5.5362624761753905	0.0
20	0.0	0.0	0.0	5.742010685132927	0.0
21	0.0	0.0	0.0	5.998894641421333	0.0
22	0.0	0.0	0.0	6.2848760431606365	0.0
23	0.0	0.0	0.0	6.6156227455936305	0.0
24	0.0	0.0	0.0	6.850468400002066	0.0
25	0.0	0.0	0.0	7.049846162322424	0.0
26	0.0	0.0	0.0	7.247330008074972	0.0
27	0.0	0.0	0.0	7.449290383896888	0.0
28	0.0	0.0	0.0	7.651767282419117	0.0
29	0.0	0.0	0.0	7.878520747856	0.0
30	0.0	0.0	0.0	8.121458591235987	0.0
31	0.0	0.0	0.0	8.34321900390319	0.0
32	0.0	0.0	0.0	8.566873333138203	0.0
33	0.0	0.0	0.0	8.803440729881011	0.0
34	0.0	0.0	0.0	9.025028968314777	0.0
35	0.0	0.0	0.0	9.294481643644103	0.0
36	1.7217423343726917E-4	0.0	0.0	9.544822979061891	0.0
37	1.7217423343726917E-4	0.0	0.0	9.793786920612183	0.0
38	1.7217423343726917E-4	0.0	0.0	10.028804749254055	0.0
39	1.7217423343726917E-4	0.0	0.0	10.27311998650154	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGTA	20	7.0304004E-4	45.000004	39
CGACGGT	20	7.0304004E-4	45.000004	28
ATCGTAG	20	7.0304004E-4	45.000004	1
ACGATTG	20	7.0304004E-4	45.000004	13
CCCGTTG	20	7.0304004E-4	45.000004	9
AAGCACG	20	7.0304004E-4	45.000004	25
TATTCGA	20	7.0304004E-4	45.000004	27
CGAATCA	20	7.0304004E-4	45.000004	43
ATGGTCG	20	7.0304004E-4	45.000004	44
TAGGCAC	20	7.0304004E-4	45.000004	10
TTCGATC	20	7.0304004E-4	45.000004	19
GCACTAG	40	6.8048394E-9	45.000004	1
TGCACGA	20	7.0304004E-4	45.000004	39
ACGAATC	20	7.0304004E-4	45.000004	42
GCGAATG	35	1.2105374E-7	45.0	1
GACGTCT	25	3.8884144E-5	45.0	20
CCAATGT	25	3.8884144E-5	45.0	15
CGTTCCA	25	3.8884144E-5	45.0	28
TATTGCG	25	3.8884144E-5	45.0	1
ACGATAG	25	3.8884144E-5	45.0	1
>>END_MODULE