SRR3553649_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553649_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1055770
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCG	4397	0.41647328490106744	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTC	3203	0.3033804711253398	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGC	2222	0.21046250603824695	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1945	0.18422573098307396	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1609	0.1524006175587486	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1491	0.14122394082044384	No Hit
GCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	1463	0.1385718480350834	TruSeq Adapter, Index 14 (95% over 21bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1154	0.1093041097966413	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGT	20	7.0330256E-4	45.0	30
TATGCGA	20	7.0330256E-4	45.0	17
CTAACGG	50	2.1827873E-11	45.0	2
GCCGAAC	20	7.0330256E-4	45.0	20
ATTCACG	20	7.0330256E-4	45.0	1
AATTGCG	50	2.1827873E-11	45.0	1
CGGTGTA	50	2.1827873E-11	45.0	21
TTACGTA	20	7.0330256E-4	45.0	31
TATCCGT	25	3.890589E-5	45.0	36
TACGCAT	60	3.6379788E-12	41.249996	16
CGGTCTA	60	3.6379788E-12	41.249996	31
GCGATAT	100	0.0	40.5	9
CACGTAG	50	1.0822987E-9	40.5	1
TTCACGG	45	1.9281288E-8	40.000004	2
TGTAACG	45	1.9281288E-8	40.000004	1
CGATGAA	645	0.0	39.069767	19
AACACGT	260	0.0	38.94231	41
TAGCCGT	35	6.2478066E-6	38.571426	44
GCCGATT	35	6.2478066E-6	38.571426	9
GTTAACG	35	6.2478066E-6	38.571426	1