##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553649_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1055770
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.928273203443933	33.0	31.0	34.0	30.0	34.0
2	32.027488941720264	33.0	31.0	34.0	30.0	34.0
3	32.02106708847571	33.0	31.0	34.0	30.0	34.0
4	35.683823181185296	37.0	35.0	37.0	33.0	37.0
5	35.586627769305814	37.0	35.0	37.0	33.0	37.0
6	35.554611326330544	37.0	35.0	37.0	33.0	37.0
7	35.839747293444596	37.0	35.0	37.0	35.0	37.0
8	35.714273942241206	37.0	35.0	37.0	35.0	37.0
9	37.40611307387026	39.0	37.0	39.0	35.0	39.0
10	36.911329172073465	39.0	37.0	39.0	33.0	39.0
11	36.84940375271129	39.0	37.0	39.0	33.0	39.0
12	36.769324758233324	39.0	35.0	39.0	33.0	39.0
13	36.71985659755439	39.0	35.0	39.0	33.0	39.0
14	37.812744253009654	40.0	37.0	41.0	33.0	41.0
15	37.95376455099122	40.0	37.0	41.0	33.0	41.0
16	38.01592960588007	40.0	37.0	41.0	33.0	41.0
17	37.921202534642966	40.0	36.0	41.0	33.0	41.0
18	37.906582873163664	40.0	36.0	41.0	33.0	41.0
19	37.84427100599562	40.0	36.0	41.0	33.0	41.0
20	37.745714502211655	40.0	36.0	41.0	33.0	41.0
21	37.6394195705504	40.0	36.0	41.0	33.0	41.0
22	37.6321556778465	40.0	36.0	41.0	33.0	41.0
23	37.52814154598066	39.0	35.0	41.0	33.0	41.0
24	37.496348636540155	39.0	35.0	41.0	32.0	41.0
25	37.35003457192381	39.0	35.0	41.0	32.0	41.0
26	37.30325923259801	39.0	35.0	41.0	32.0	41.0
27	37.27206114968222	39.0	35.0	41.0	32.0	41.0
28	37.1570834556769	39.0	35.0	41.0	32.0	41.0
29	37.130359832160416	39.0	35.0	41.0	32.0	41.0
30	36.97409568371899	39.0	35.0	41.0	31.0	41.0
31	36.916969605122326	39.0	35.0	41.0	31.0	41.0
32	36.765795580476805	39.0	35.0	41.0	31.0	41.0
33	36.66392017200716	39.0	35.0	41.0	31.0	41.0
34	36.53518853538176	39.0	35.0	41.0	30.0	41.0
35	36.42010665201701	39.0	35.0	41.0	30.0	41.0
36	36.266566581736555	39.0	35.0	41.0	30.0	41.0
37	36.22261761557915	39.0	35.0	41.0	30.0	41.0
38	36.10631955823712	39.0	35.0	40.0	30.0	41.0
39	36.0230608939447	38.0	35.0	40.0	29.0	41.0
40	35.89291133485513	38.0	35.0	40.0	29.0	41.0
41	35.7513776674844	38.0	35.0	40.0	28.0	41.0
42	35.65845307216534	38.0	35.0	40.0	28.0	41.0
43	35.60131183875276	38.0	35.0	40.0	28.0	41.0
44	35.547718726616594	38.0	34.0	40.0	28.0	41.0
45	35.464184434109704	38.0	34.0	40.0	28.0	41.0
46	35.307321670439585	38.0	34.0	40.0	27.0	41.0
47	35.1733341542192	37.0	34.0	40.0	27.0	41.0
48	35.09829413603342	37.0	34.0	40.0	26.0	41.0
49	35.025265919660534	37.0	34.0	40.0	26.0	41.0
50	34.89017210187825	37.0	34.0	40.0	26.0	41.0
51	33.47225342640916	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	10.0
11	20.0
12	15.0
13	24.0
14	48.0
15	80.0
16	144.0
17	276.0
18	483.0
19	936.0
20	1572.0
21	2753.0
22	4076.0
23	5234.0
24	6683.0
25	8078.0
26	10154.0
27	11826.0
28	13876.0
29	16714.0
30	20361.0
31	25715.0
32	33294.0
33	45270.0
34	75253.0
35	89010.0
36	90337.0
37	126182.0
38	198020.0
39	269234.0
40	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.09833581177718	18.49020146433409	22.495051005427317	14.916411718461408
2	29.86199645756178	26.853102474970875	25.49030565369351	17.794595413773834
3	31.932712617331426	25.683908427024825	25.766218020970477	16.617160934673272
4	28.571469164685492	25.33705257773947	26.886443069986832	19.20503518758821
5	25.709861049281567	29.79796736031522	23.89687147768927	20.595300112713943
6	24.426342858766585	36.90424997868854	24.02682402417193	14.642583138372942
7	81.31572217433721	6.0410884946531915	8.651694971442645	3.9914943595669508
8	82.16069787927294	6.880475861219773	7.03259232598009	3.9262339335271887
9	76.87299317086108	7.232825331274804	8.629436335565511	7.264745162298607
10	41.479867774231124	25.457154493876505	16.156643966015324	16.906333765877037
11	31.784669009348626	25.317256599448747	22.95405249249363	19.944021898709
12	27.511200356138172	22.378074770073027	28.843971698381278	21.266753175407523
13	24.8733152106993	24.008354092273883	30.217566325999034	20.90076437102778
14	21.317048220729895	27.725451566155506	28.172045047690315	22.78545516542429
15	20.715970334447846	25.133504456463058	31.425026284133857	22.725498924955247
16	22.936908606988265	23.693133921213903	29.490892902810273	23.879064568987562
17	23.378008467753393	24.45295850421967	27.654413366547637	24.5146196614793
18	23.794292317455508	24.468113320136016	27.94879566572265	23.78879869668583
19	25.225854115953283	26.319274084317605	25.56579558047681	22.8890762192523
20	26.405182947043386	24.993606562035293	26.248330602309217	22.352879888612105
21	25.303617265124036	27.048694317891208	25.276528031673568	22.371160385311196
22	24.28388758915294	23.562991939532285	26.32211561230192	25.83100485901285
23	23.645680403875843	26.940432101688817	25.895602261856276	23.518285232579064
24	23.383217935724637	25.896833590649475	27.387499171221002	23.33244930240488
25	23.70952006592345	27.061860064218536	26.46078217793648	22.767837691921535
26	21.99588925618269	26.53930306790305	27.001051365354193	24.463756310560065
27	23.84354546918363	25.348323972077253	26.709794746961933	24.098335811777186
28	21.544086306676643	26.982486715856673	28.59884255093439	22.874584426532294
29	23.220114229424972	24.77566136563835	27.33796186669445	24.666262538242233
30	23.19444575996666	25.954043020733682	27.113860026331494	23.737651192968166
31	24.976841546927837	25.887930136298625	25.25029125661839	23.88493706015515
32	24.706043930022638	26.36483324966612	25.90640006819667	23.02272275211457
33	24.51622986067041	24.94738437349044	25.29746062115802	25.238925144681133
34	22.859429610615948	25.729751745171768	26.497532606533618	24.91328603767866
35	24.353126154370745	25.167886945073263	26.192920806615078	24.286066093940914
36	24.69221516049897	26.474516229860672	25.522604355115224	23.310664254525136
37	23.66007747899637	26.459456131543803	27.694478911126474	22.18598747833335
38	23.485702378358923	27.429743220587817	26.36691703685462	22.71763736419864
39	24.24817905415005	25.25635318298493	26.676548869545453	23.81891889331957
40	24.988112941265616	25.694422080566792	26.748913115545996	22.568551862621593
41	22.030840050389763	25.27397065648768	27.180257063564984	25.514932229557573
42	22.250490163577293	25.842844558947498	26.369663847239455	25.53700143023575
43	22.499692167801697	23.95749074135465	27.737764854087537	25.80505223675611
44	23.42887181867263	24.456936643397707	27.54340433996041	24.570787197969253
45	23.765119296816543	24.72792369550186	27.00266156454531	24.50429544313629
46	24.410525019653903	26.141962738096364	26.10776968468511	23.339742557564623
47	22.74330583365695	25.356280250433333	28.9193669075651	22.98104700834462
48	22.656828665334306	25.623478598558396	27.30196917889313	24.417723557214167
49	22.285725110582796	25.125832330905407	28.41802665353249	24.170415904979304
50	22.357805203784913	24.252725498924953	29.135038881574587	24.254430415715543
51	22.751546264811466	24.35066349678434	26.943747217670516	25.954043020733682
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2241.0
1	2681.5
2	3122.0
3	3788.5
4	4455.0
5	3330.0
6	2205.0
7	2215.5
8	2226.0
9	2247.5
10	2269.0
11	2304.0
12	2339.0
13	2325.5
14	2312.0
15	2564.5
16	2817.0
17	2707.5
18	2598.0
19	2746.5
20	2895.0
21	2907.0
22	2919.0
23	3162.5
24	3406.0
25	4622.5
26	6963.0
27	8087.0
28	10463.0
29	12839.0
30	14692.0
31	16545.0
32	18530.0
33	20515.0
34	23654.5
35	26794.0
36	29285.0
37	31776.0
38	32798.5
39	33821.0
40	37095.5
41	40370.0
42	44388.5
43	48407.0
44	55787.5
45	63168.0
46	73454.5
47	83741.0
48	83907.0
49	84073.0
50	82726.5
51	81380.0
52	74973.0
53	68566.0
54	64886.5
55	61207.0
56	59425.5
57	57644.0
58	54754.0
59	51864.0
60	50349.5
61	48835.0
62	45082.5
63	41330.0
64	37881.5
65	34433.0
66	30622.0
67	26811.0
68	23807.5
69	20804.0
70	18350.0
71	15896.0
72	14720.0
73	13544.0
74	11047.0
75	7259.5
76	5969.0
77	4681.0
78	3393.0
79	2570.5
80	1748.0
81	1370.5
82	993.0
83	790.5
84	588.0
85	380.5
86	173.0
87	123.5
88	74.0
89	75.0
90	76.0
91	51.5
92	27.0
93	28.5
94	30.0
95	40.0
96	50.0
97	28.0
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1055770.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.155979722618664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.19599002842693	16.660408302920622
2	8.849127231511376	3.9212216700847744
3	3.294858967875621	2.1900248544342156
4	1.7073784749845171	1.5131457148237013
5	1.026985403227887	1.1376933884671205
6	0.736948617748261	0.9796691178865393
7	0.5513286688106319	0.855065876466669
8	0.455887113716271	0.808050051784068
9	0.4067880500846101	0.8111509010170387
>10	5.473960847011585	30.202742251210406
>50	2.0542380327574774	30.98617372080098
>100	0.23532318398450366	7.048373473900779
>500	0.00731351760098092	1.1231149964565552
>1k	0.0038718622593428395	1.7631656797465294
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCG	4397	0.41647328490106744	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTC	3203	0.3033804711253398	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGC	2222	0.21046250603824695	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1945	0.18422573098307396	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1609	0.1524006175587486	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1491	0.14122394082044384	No Hit
GCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	1463	0.1385718480350834	TruSeq Adapter, Index 14 (95% over 21bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1154	0.1093041097966413	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06734421322825994	0.0
2	0.0	0.0	0.0	0.3902365098458945	0.0
3	0.0	0.0	0.0	0.5095806851871146	0.0
4	0.0	0.0	0.0	0.7191907328300672	0.0
5	0.0	0.0	0.0	1.5216382356005569	0.0
6	0.0	0.0	0.0	2.024967559222179	0.0
7	0.0	0.0	0.0	2.4778124023224755	0.0
8	0.0	0.0	0.0	3.077280089413414	0.0
9	0.0	0.0	0.0	3.3166314632921945	0.0
10	0.0	0.0	0.0	4.254809286113453	0.0
11	0.0	0.0	0.0	4.8977523513644075	0.0
12	9.471759947715885E-5	0.0	0.0	5.6278356081343475	0.0
13	9.471759947715885E-5	0.0	0.0	5.82911050702331	0.0
14	9.471759947715885E-5	0.0	0.0	5.912746147361641	0.0
15	1.894351989543177E-4	0.0	0.0	6.093277891965106	0.0
16	1.894351989543177E-4	0.0	0.0	6.396468927891491	0.0
17	1.894351989543177E-4	0.0	0.0	6.76122640347803	0.0
18	1.894351989543177E-4	0.0	0.0	7.212745200185647	0.0
19	1.894351989543177E-4	0.0	0.0	7.483637534690321	0.0
20	1.894351989543177E-4	0.0	0.0	7.701582731087264	0.0
21	1.894351989543177E-4	0.0	0.0	7.9753165935762524	0.0
22	3.788703979086354E-4	0.0	0.0	8.276329124714664	0.0
23	3.788703979086354E-4	0.0	0.0	8.582266971025886	0.0
24	3.788703979086354E-4	0.0	0.0	8.829764058459702	0.0
25	3.788703979086354E-4	0.0	0.0	9.042783939683833	0.0
26	3.788703979086354E-4	0.0	0.0	9.23752332420887	0.0
27	3.788703979086354E-4	0.0	0.0	9.456794566998495	0.0
28	3.788703979086354E-4	0.0	0.0	9.676539397785502	0.0
29	4.7358799738579427E-4	0.0	0.0	9.897515557365715	0.0
30	4.7358799738579427E-4	0.0	0.0	10.15202174716084	0.0
31	4.7358799738579427E-4	0.0	0.0	10.384648171476742	0.0
32	4.7358799738579427E-4	0.0	0.0	10.616516854996828	0.0
33	4.7358799738579427E-4	0.0	0.0	10.862972048836394	0.0
34	4.7358799738579427E-4	0.0	0.0	11.094082991560661	0.0
35	4.7358799738579427E-4	0.0	0.0	11.357682070905595	0.0
36	6.630231963401119E-4	0.0	0.0	11.598075338378624	0.0
37	6.630231963401119E-4	0.0	0.0	11.836858406660541	0.0
38	6.630231963401119E-4	0.0	0.0	12.071663335764418	0.0
39	6.630231963401119E-4	0.0	0.0	12.30798374645993	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGT	20	7.0330256E-4	45.0	30
TATGCGA	20	7.0330256E-4	45.0	17
CTAACGG	50	2.1827873E-11	45.0	2
GCCGAAC	20	7.0330256E-4	45.0	20
ATTCACG	20	7.0330256E-4	45.0	1
AATTGCG	50	2.1827873E-11	45.0	1
CGGTGTA	50	2.1827873E-11	45.0	21
TTACGTA	20	7.0330256E-4	45.0	31
TATCCGT	25	3.890589E-5	45.0	36
TACGCAT	60	3.6379788E-12	41.249996	16
CGGTCTA	60	3.6379788E-12	41.249996	31
GCGATAT	100	0.0	40.5	9
CACGTAG	50	1.0822987E-9	40.5	1
TTCACGG	45	1.9281288E-8	40.000004	2
TGTAACG	45	1.9281288E-8	40.000004	1
CGATGAA	645	0.0	39.069767	19
AACACGT	260	0.0	38.94231	41
TAGCCGT	35	6.2478066E-6	38.571426	44
GCCGATT	35	6.2478066E-6	38.571426	9
GTTAACG	35	6.2478066E-6	38.571426	1
>>END_MODULE