##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553648_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1481628
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.941542681428807	33.0	31.0	34.0	30.0	34.0
2	32.044594189634644	33.0	31.0	34.0	30.0	34.0
3	32.03408750374588	33.0	31.0	34.0	30.0	34.0
4	35.69123828653346	37.0	35.0	37.0	33.0	37.0
5	35.595990356553735	37.0	35.0	37.0	33.0	37.0
6	35.56158765898053	37.0	35.0	37.0	33.0	37.0
7	35.89429870385819	37.0	35.0	37.0	35.0	37.0
8	35.840086715423844	37.0	35.0	37.0	35.0	37.0
9	37.55122608374032	39.0	37.0	39.0	35.0	39.0
10	36.96760725364261	39.0	37.0	39.0	33.0	39.0
11	36.90861876260438	39.0	37.0	39.0	33.0	39.0
12	36.740822257678715	39.0	35.0	39.0	33.0	39.0
13	36.70828845027227	39.0	35.0	39.0	33.0	39.0
14	37.60370079399147	40.0	36.0	41.0	32.0	41.0
15	37.77939806753112	40.0	36.0	41.0	33.0	41.0
16	37.862636235276334	40.0	36.0	41.0	33.0	41.0
17	37.84032631672728	40.0	36.0	41.0	33.0	41.0
18	37.83619977484227	40.0	36.0	41.0	33.0	41.0
19	37.79605272038595	40.0	36.0	41.0	33.0	41.0
20	37.716076505033655	40.0	36.0	41.0	33.0	41.0
21	37.5774978604616	40.0	35.0	41.0	33.0	41.0
22	37.62308352703918	39.0	35.0	41.0	33.0	41.0
23	37.562967222541694	39.0	35.0	41.0	33.0	41.0
24	37.50885647409471	39.0	35.0	41.0	33.0	41.0
25	37.38765398602078	39.0	35.0	41.0	32.0	41.0
26	37.34682052445013	39.0	35.0	41.0	32.0	41.0
27	37.31500079642123	39.0	35.0	41.0	32.0	41.0
28	37.195022637261175	39.0	35.0	41.0	32.0	41.0
29	37.15216977540921	39.0	35.0	41.0	32.0	41.0
30	36.97305463989611	39.0	35.0	41.0	31.0	41.0
31	36.880876306333306	39.0	35.0	41.0	31.0	41.0
32	36.764316684079944	39.0	35.0	41.0	31.0	41.0
33	36.676498419306334	39.0	35.0	41.0	31.0	41.0
34	36.50343068570518	39.0	35.0	41.0	30.0	41.0
35	36.41251582718469	39.0	35.0	41.0	30.0	41.0
36	36.26435650514164	39.0	35.0	41.0	30.0	41.0
37	36.120062525816195	39.0	35.0	41.0	29.0	41.0
38	35.97959339321341	39.0	35.0	41.0	29.0	41.0
39	35.856651602156546	38.0	35.0	40.0	29.0	41.0
40	35.72991601130648	38.0	35.0	40.0	28.0	41.0
41	35.582084031889245	38.0	35.0	40.0	27.0	41.0
42	35.51366807322756	38.0	34.0	40.0	27.0	41.0
43	35.49337418029357	38.0	34.0	40.0	27.0	41.0
44	35.35812295663959	38.0	34.0	40.0	27.0	41.0
45	35.285000688431914	38.0	34.0	40.0	27.0	41.0
46	35.178435477731256	38.0	34.0	40.0	26.0	41.0
47	35.02470255691712	37.0	34.0	40.0	26.0	41.0
48	34.94390899740016	37.0	34.0	40.0	26.0	41.0
49	34.90455836417778	37.0	34.0	40.0	26.0	41.0
50	34.77025474680554	37.0	34.0	40.0	25.0	41.0
51	33.318048794974175	35.0	31.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	9.0
10	14.0
11	14.0
12	17.0
13	34.0
14	77.0
15	96.0
16	203.0
17	405.0
18	765.0
19	1353.0
20	2178.0
21	3259.0
22	4714.0
23	6657.0
24	8805.0
25	11902.0
26	15446.0
27	19192.0
28	22404.0
29	26513.0
30	30766.0
31	37324.0
32	46943.0
33	64454.0
34	106063.0
35	119013.0
36	123280.0
37	173670.0
38	278487.0
39	377454.0
40	113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.547619240457124	18.137683683083743	22.960081747915133	14.354615328544007
2	30.876711293253095	24.117659763449396	25.630050188036403	19.375578755261106
3	32.625800808300056	24.845035326006258	25.521048468306486	17.0081153973872
4	28.243864181832418	25.967314332612506	25.118450785217345	20.670370700337738
5	24.98353162872192	29.712181465253085	25.14619054175542	20.158096364269575
6	24.87412494904254	34.909842416585	24.902877105454273	15.313155528918191
7	83.29486213813453	5.554903120081424	7.106034713166867	4.04420002861717
8	85.31439740609653	4.559241591006649	6.357668726562943	3.76869227633387
9	80.18470223294915	6.290445374952418	8.338867785975967	5.185984606122455
10	41.76473446776114	25.439111571865542	16.63818448355458	16.157969476818742
11	32.797098866922056	26.790800389841447	21.30993744718647	19.10216329605002
12	27.444473241596405	23.87650611354537	28.47624369949812	20.202776945360103
13	26.151166149667798	24.182183382063513	29.281304079026583	20.385346389242105
14	21.859603085254868	26.416279929914932	30.490649474766947	21.233467510063257
15	21.48757987835003	25.22016322585696	32.15240262738015	21.139854268412854
16	26.216094728231376	24.159033171619328	29.133831164097874	20.491040936051423
17	25.754237905871108	24.048479105416472	26.54957924661251	23.647703742099907
18	27.160326343724606	23.729235678591387	27.44150353530036	21.668934442383648
19	26.350676418102246	26.162909988202166	25.030641969509215	22.45577162418637
20	26.35526596419614	27.028444386850143	25.039753568372085	21.57653608058163
21	25.897391248005576	26.38246577413494	27.22282516259142	20.497317815268072
22	24.50925603457818	23.744219196721446	27.597278129193022	24.149246639507354
23	23.637849716663023	26.797212255707908	27.651002815821514	21.91393521180755
24	23.19043646583353	26.66296803246159	27.91503670287009	22.231558798834794
25	25.322348119770954	25.828953016546663	25.98270281069202	22.865996052990358
26	22.529609321638087	25.96225233324424	26.30802063675902	25.200117708358643
27	22.733709136166432	25.254517328236236	27.965926669852355	24.045846865744977
28	21.257225160431634	26.91154594810573	27.87973769394207	23.951491197520568
29	23.409114838542468	26.45488611176355	26.554978712605326	23.581020337088663
30	24.640125591578993	24.833831434071172	28.13722472847435	22.388818245875484
31	25.42959501305321	26.084415251331645	25.80445293960427	22.681536796010874
32	25.678712875296632	25.3226180930706	26.077193465566257	22.921475566066515
33	25.604942671169823	24.656863936156714	25.293258496734673	24.44493489593879
34	22.705969379628353	25.77603824981709	28.45714308854854	23.06084928200601
35	22.48000172782912	26.668569978429137	27.32460509655595	23.5268231971858
36	24.80433685108543	27.488343902788014	25.23852141023253	22.46879783589403
37	23.047148137049245	27.81055703590915	25.68303244809088	23.459262378950722
38	23.64824368869919	27.39317831466468	25.70314545891411	23.255432537722022
39	24.418814979198558	24.710318649485565	25.888482129117428	24.982384242198446
40	25.325047852767362	24.449524442032683	27.783222239320533	22.442205465879425
41	21.249058468117504	25.171298058622003	27.29261326054853	26.287030212711965
42	22.44085559938122	25.372698140153936	27.782547306071432	24.403898954393412
43	22.12842899837206	24.11624240362628	28.196551361070387	25.558777236931267
44	23.73072053173941	24.79293047917561	27.513046459705137	23.963302529379842
45	23.789979671010535	24.453776521502025	26.093931810143978	25.662311997343462
46	22.995313263518238	25.01248626510838	27.099312378005813	24.892888093367567
47	21.745606859481597	26.430386034821158	28.37621859198125	23.447788513715995
48	21.667382095910714	26.868080246863585	27.65829209491181	23.806245562313887
49	23.684150137551395	25.36567883436328	27.92597062150553	23.02420040657979
50	21.89604948070636	25.88389258302354	28.150993366756026	24.069064569514076
51	22.340897985189265	24.949177526342645	26.4067633711026	26.30316111736549
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2778.0
1	3186.5
2	3595.0
3	4733.0
4	5871.0
5	4384.5
6	2898.0
7	2726.5
8	2555.0
9	2702.5
10	2850.0
11	2751.0
12	2652.0
13	3039.0
14	3426.0
15	3486.5
16	3547.0
17	3389.0
18	3231.0
19	3472.5
20	3714.0
21	3943.0
22	4172.0
23	4726.0
24	5280.0
25	6933.0
26	9677.0
27	10768.0
28	13762.0
29	16756.0
30	19832.0
31	22908.0
32	25435.5
33	27963.0
34	31074.5
35	34186.0
36	38677.5
37	43169.0
38	46953.5
39	50738.0
40	55790.0
41	60842.0
42	66073.5
43	71305.0
44	77620.5
45	83936.0
46	92510.0
47	101084.0
48	107979.5
49	114875.0
50	118539.0
51	122203.0
52	116492.5
53	110782.0
54	103782.5
55	96783.0
56	91753.0
57	86723.0
58	82022.0
59	77321.0
60	72942.0
61	68563.0
62	63403.0
63	58243.0
64	51472.5
65	44702.0
66	39898.5
67	35095.0
68	30100.0
69	25105.0
70	21884.0
71	18663.0
72	17056.0
73	15449.0
74	13902.5
75	9707.5
76	7059.0
77	5579.5
78	4100.0
79	3219.5
80	2339.0
81	1765.5
82	1192.0
83	886.5
84	581.0
85	440.5
86	300.0
87	231.0
88	162.0
89	143.5
90	125.0
91	75.5
92	26.0
93	31.0
94	36.0
95	26.5
96	17.0
97	15.5
98	14.0
99	9.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1481628.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.21800113974848
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.73897457393322	17.35289596841214
2	9.198546138121067	4.271437094378477
3	3.58432155275187	2.4966234569105388
4	1.8776824482577295	1.743841328949347
5	1.152542827684051	1.3379870343388616
6	0.7826215783146987	1.0902545218381459
7	0.6028131481889814	0.9797281453194997
8	0.4704344891876928	0.8738038804909478
9	0.40680513439455623	0.8500681866625507
>10	5.057686465160613	28.67073718526288
>50	1.84391603053469	29.786321432379655
>100	0.2760446269394193	8.700410100396109
>500	0.004976414270573401	0.8720731967033059
>1k	0.0026345722608918007	0.973818467957678
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2874	0.1939758157918182	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2265	0.15287238092152686	No Hit
GCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	1816	0.12256787803686216	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06614345841196306	0.0
2	0.0	0.0	0.0	0.3679736074102271	0.0
3	0.0	0.0	0.0	0.49202633859511297	0.0
4	0.0	0.0	0.0	0.6638643438163966	0.0
5	0.0	0.0	0.0	1.217377101404671	0.0
6	0.0	0.0	0.0	1.7609683402311511	0.0
7	0.0	0.0	0.0	2.188943513486516	0.0
8	0.0	0.0	0.0	2.8092746627358554	0.0
9	0.0	0.0	0.0	3.092679134033644	0.0
10	0.0	0.0	0.0	3.714562629755917	0.0
11	0.0	0.0	0.0	4.451927204399485	0.0
12	0.0	0.0	0.0	5.038916651143202	0.0
13	6.749332491016638E-5	0.0	0.0	5.26542424954172	0.0
14	6.749332491016638E-5	0.0	0.0	5.363087090686731	0.0
15	6.749332491016638E-5	0.0	0.0	5.529188163290651	0.0
16	6.749332491016638E-5	0.0	0.0	5.859500495401005	0.0
17	6.749332491016638E-5	0.0	0.0	6.251704206453982	0.0
18	6.749332491016638E-5	0.0	0.0	6.72166022780347	0.0
19	6.749332491016638E-5	0.0	0.0	6.97725744923827	0.0
20	6.749332491016638E-5	0.0	0.0	7.249323041951151	0.0
21	6.749332491016638E-5	0.0	0.0	7.576868147740188	0.0
22	1.3498664982033277E-4	0.0	0.0	7.937012529460836	0.0
23	1.3498664982033277E-4	0.0	0.0	8.2706995278167	0.0
24	1.3498664982033277E-4	0.0	0.0	8.545127386901436	0.0
25	1.3498664982033277E-4	0.0	0.0	8.797349942090728	0.0
26	1.3498664982033277E-4	0.0	0.0	9.042958151438823	0.0
27	1.3498664982033277E-4	0.0	0.0	9.34175110081613	0.0
28	1.3498664982033277E-4	0.0	0.0	9.615976479926136	0.0
29	2.0247997473049915E-4	0.0	0.0	9.931642760530984	0.0
30	2.6997329964066554E-4	0.0	0.0	10.318649485565878	0.0
31	3.374666245508319E-4	0.0	0.0	10.600839077015284	0.0
32	3.374666245508319E-4	0.0	0.0	10.888293147807682	0.0
33	3.374666245508319E-4	0.0	0.0	11.173789912177686	0.0
34	4.049599494609983E-4	0.0	0.0	11.485069126663372	0.0
35	4.049599494609983E-4	0.0	0.0	11.824763030936241	0.0
36	4.724532743711647E-4	0.0	0.0	12.118763954244924	0.0
37	4.724532743711647E-4	0.0	0.0	12.423158849589775	0.0
38	6.074399241914975E-4	0.0	0.0	12.780670991638926	0.0
39	6.074399241914975E-4	0.0	0.0	13.425434724505747	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	60	0.0	45.000004	1
CGAAACG	20	7.0339476E-4	45.0	40
TAGGTCG	40	6.8175723E-9	45.0	1
ACTCGAC	20	7.0339476E-4	45.0	10
TCGACGT	25	3.891354E-5	45.0	26
TATCGTG	20	7.0339476E-4	45.0	1
ACGAAGT	20	7.0339476E-4	45.0	35
CGGTCTA	75	0.0	42.000004	31
TCGTAAG	60	3.6379788E-12	41.250004	1
TATACGG	50	1.0822987E-9	40.5	2
ACGTAAC	45	1.9290383E-8	40.0	15
CTTACGG	90	0.0	40.0	2
CGTAAGG	270	0.0	39.166664	2
CGTTAGG	195	0.0	38.076923	2
TTAGGGA	775	0.0	37.741936	4
ACGTTAT	30	1.14008115E-4	37.500004	39
CGACTCG	30	1.14008115E-4	37.500004	17
GCTTACG	90	0.0	37.5	1
TACGCGG	170	0.0	37.058823	2
GTCAACG	55	2.750312E-9	36.818184	1
>>END_MODULE