FastQCFastQC Report
Sat 18 Jun 2016
SRR3553644_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553644_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850401
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC20960.24647195852309675No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17860.21001856771099753No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17180.20202234004898867No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT9820.11547493476606917No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9500.11171200410159442No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT9320.10959535560282738No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAG302.164832E-645.0000041
GTCGACG351.2114288E-745.0000041
TATAGCG302.164832E-645.0000041
CGTCCTA207.032249E-445.00000413
CGTTTCA207.032249E-445.00000444
TAGGTCG253.8899474E-545.05
AATGCGC253.8899474E-545.026
CGAATAT900.045.014
TCGACGT551.8189894E-1245.026
TAGTCAA253.8899474E-545.024
TAGTACG502.1827873E-1145.01
GTACGAG551.8189894E-1245.01
TGCGTAG700.041.7857171
TTTCGAC603.6379788E-1241.25000424
TACGCAT556.002665E-1140.90909216
CTACGAA1000.040.511
CTTACGG501.0804797E-940.52
TCGTTCA1200.039.37500416
GTATACG403.4568075E-739.3750041
TCGTGTA3400.039.0441217