##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553644_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	850401
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96983423114507	33.0	31.0	34.0	30.0	34.0
2	32.06310199541158	33.0	31.0	34.0	30.0	34.0
3	32.05145572500503	33.0	31.0	34.0	30.0	34.0
4	35.71373387378425	37.0	35.0	37.0	33.0	37.0
5	35.62303195786458	37.0	35.0	37.0	33.0	37.0
6	35.59933960566838	37.0	35.0	37.0	33.0	37.0
7	35.90725669419486	37.0	35.0	37.0	35.0	37.0
8	35.83567516971405	37.0	35.0	37.0	35.0	37.0
9	37.537172463343765	39.0	37.0	39.0	35.0	39.0
10	37.001742707263986	39.0	37.0	39.0	33.0	39.0
11	36.91980489204505	39.0	37.0	39.0	33.0	39.0
12	36.846356013222	39.0	35.0	39.0	33.0	39.0
13	36.82828101095836	39.0	37.0	39.0	33.0	39.0
14	37.92294929098155	40.0	37.0	41.0	33.0	41.0
15	38.05327957046146	40.0	37.0	41.0	33.0	41.0
16	38.07635103909803	40.0	37.0	41.0	33.0	41.0
17	38.02570904784919	40.0	37.0	41.0	33.0	41.0
18	37.97847015702004	40.0	37.0	41.0	33.0	41.0
19	37.899986006601594	40.0	36.0	41.0	33.0	41.0
20	37.84968503094422	40.0	36.0	41.0	33.0	41.0
21	37.682764954415624	40.0	36.0	41.0	33.0	41.0
22	37.666577297063384	40.0	36.0	41.0	33.0	41.0
23	37.606531506900865	39.0	36.0	41.0	33.0	41.0
24	37.569760618813945	39.0	35.0	41.0	33.0	41.0
25	37.417888737195746	39.0	35.0	41.0	32.0	41.0
26	37.39184455333425	39.0	35.0	41.0	32.0	41.0
27	37.34913646620829	39.0	35.0	41.0	32.0	41.0
28	37.23798067029554	39.0	35.0	41.0	32.0	41.0
29	37.18394381003785	39.0	35.0	41.0	32.0	41.0
30	37.02640871776962	39.0	35.0	41.0	31.0	41.0
31	36.97295628768075	39.0	35.0	41.0	31.0	41.0
32	36.862087415231166	39.0	35.0	41.0	31.0	41.0
33	36.75768843169281	39.0	35.0	41.0	31.0	41.0
34	36.6551027103684	39.0	35.0	41.0	31.0	41.0
35	36.542260651151636	39.0	35.0	41.0	30.0	41.0
36	36.394074089752955	39.0	35.0	41.0	30.0	41.0
37	36.301727067583414	39.0	35.0	41.0	30.0	41.0
38	36.171417954588485	39.0	35.0	41.0	30.0	41.0
39	36.06693430511018	38.0	35.0	40.0	29.0	41.0
40	35.94854309907914	38.0	35.0	40.0	29.0	41.0
41	35.828896014938834	38.0	35.0	40.0	29.0	41.0
42	35.725710576539775	38.0	35.0	40.0	28.0	41.0
43	35.64665610694249	38.0	34.0	40.0	28.0	41.0
44	35.500997764584	38.0	34.0	40.0	28.0	41.0
45	35.42902936379426	38.0	34.0	40.0	28.0	41.0
46	35.2822880029539	38.0	34.0	40.0	27.0	41.0
47	35.18484456156566	37.0	34.0	40.0	27.0	41.0
48	35.08448602482829	37.0	34.0	40.0	27.0	41.0
49	34.99570555537917	37.0	34.0	40.0	27.0	41.0
50	34.84082450514522	37.0	34.0	40.0	26.0	41.0
51	33.358296850544626	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	6.0
10	9.0
11	8.0
12	11.0
13	12.0
14	33.0
15	51.0
16	116.0
17	227.0
18	395.0
19	666.0
20	1097.0
21	1699.0
22	2462.0
23	3427.0
24	4634.0
25	6231.0
26	7979.0
27	9644.0
28	11447.0
29	13613.0
30	16833.0
31	20559.0
32	26515.0
33	36032.0
34	60061.0
35	72193.0
36	72956.0
37	103330.0
38	162903.0
39	215208.0
40	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.261589532467625	18.063831063227816	23.003265518267266	14.671313886037293
2	30.555702545034634	24.94293868422074	26.33863318599108	18.162725584753545
3	32.96339021238216	24.09486818571474	26.116737868370333	16.825003733532768
4	29.118263031205277	26.376262492635828	25.26831459511454	19.237159881044356
5	24.22210227880729	31.23197174039071	24.776781777067526	19.769144203734474
6	24.475041774409956	36.92646175157367	24.04500935441045	14.553487119605926
7	82.90724023137321	6.333600266227345	6.923086873133968	3.836072629265488
8	85.25766079767074	5.225769960289322	6.540679044356722	2.9758901976832104
9	79.78059762394447	7.469299777399133	7.962126102862062	4.787976495794337
10	41.84837506070666	26.124851687615607	15.788669110219766	16.23810414145797
11	31.28030188111256	27.55217832528419	21.895317620745978	19.272202172857273
12	27.489854786153828	24.443409638511714	28.24467515913081	19.822060416203648
13	25.13096762586121	25.497970957230763	29.03806557141866	20.332995845489364
14	22.15401910392862	28.254317668958528	28.656833658474063	20.934829568638797
15	22.197880764486403	26.55700075611388	30.209865698652756	21.035252780746966
16	24.06452955723241	24.802534333802523	29.555350946200676	21.57758516276439
17	24.8598014348525	23.943292634886365	26.915184718738573	24.281721211522562
18	25.326757611997163	25.021842636591447	27.55100240945154	22.10039734195985
19	26.098746356130814	26.53583427112621	25.456696311504807	21.90872306123817
20	27.08310549964076	25.358154564728874	26.255848711372636	21.302891224257735
21	26.41424457403037	26.125204462365403	26.78113031381666	20.679420649787573
22	25.06382283181699	24.83875254144809	25.775957460068838	24.32146716666608
23	23.90542814507509	27.399897224956227	25.43188448743593	23.262790142532758
24	23.545245125534894	26.84733437519476	27.51984063988636	22.087579859383986
25	24.513494222137556	26.468336702332195	26.47398109832891	22.544187977201343
26	23.33628488207328	26.8127624497149	26.07675673006029	23.774195938151532
27	24.411895094196737	26.108271274375266	26.578990382184404	22.90084324924359
28	22.72327995851369	27.21128032539943	28.414712588531764	21.65072712755512
29	23.920832642482782	26.21480924881321	26.36603202489179	23.498326083812223
30	23.804064200300797	26.590279174177827	27.47739007832775	22.12826654719362
31	26.02548679975682	26.37202919563829	25.11285852203843	22.48962548256646
32	24.64413847114479	26.45269702175797	25.79641839555692	23.10674611154032
33	24.32934580274482	26.506201192143468	24.85321630618967	24.311236698922038
34	23.6713033027948	27.093335967384796	25.94987541171753	23.28548531810287
35	24.18600166274499	25.837575449699614	26.614150265580587	23.362272621974807
36	25.682472151373293	28.018546544512528	24.849100600775397	21.449880703338778
37	24.162836120841817	27.063820479985324	26.680471918541958	22.0928714806309
38	23.309826775838694	28.046180566579764	26.27054765928074	22.3734449983008
39	24.119092051867298	26.070994742480313	27.169064947007353	22.64084825864504
40	23.531604501876174	25.630731854736766	28.694815739868602	22.142847903518458
41	22.30077339984313	25.995971312357348	27.505847241477845	24.197408046321677
42	23.51549445496889	26.20422600631937	26.70504855944431	23.575230979267428
43	24.013259626928942	24.60568602341719	27.509727763725582	23.871326585928283
44	24.208344063565306	24.964693127124733	27.24526429296297	23.581698516346993
45	24.49514993514824	25.547829788535054	26.17353460308725	23.783485673229453
46	23.437060868931244	26.74691116308659	26.51619647672098	23.29983149126118
47	23.163778029423764	26.281366084941105	28.127318759032505	22.427537126602626
48	22.88449801916978	26.866384211683663	26.79477093747538	23.454346831671177
49	23.01408394392763	26.951755701133933	27.60086124075583	22.433299114182603
50	22.592635709506457	26.311704713423435	28.489736018654728	22.605923558415384
51	22.005265751098598	26.012316542431158	27.261256748286982	24.72116095818326
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2301.0
1	2780.0
2	3259.0
3	3655.5
4	4052.0
5	3005.5
6	1959.0
7	1941.5
8	1924.0
9	2045.5
10	2167.0
11	2161.0
12	2155.0
13	2251.5
14	2348.0
15	2337.5
16	2327.0
17	2286.5
18	2246.0
19	2274.0
20	2302.0
21	2578.0
22	2854.0
23	3062.0
24	3270.0
25	4071.0
26	6207.0
27	7542.0
28	9100.5
29	10659.0
30	13289.0
31	15919.0
32	17642.0
33	19365.0
34	20187.0
35	21009.0
36	23797.0
37	26585.0
38	27093.5
39	27602.0
40	31320.5
41	35039.0
42	37337.5
43	39636.0
44	44473.0
45	49310.0
46	53200.5
47	57091.0
48	62097.5
49	67104.0
50	64982.5
51	62861.0
52	60093.5
53	57326.0
54	53824.5
55	50323.0
56	47512.5
57	44702.0
58	43829.5
59	42957.0
60	41157.5
61	39358.0
62	36545.0
63	33732.0
64	29782.5
65	25833.0
66	23388.5
67	20944.0
68	19275.0
69	17606.0
70	15596.5
71	13587.0
72	11189.0
73	8791.0
74	7719.0
75	5473.0
76	4299.0
77	3644.0
78	2989.0
79	2307.5
80	1626.0
81	1356.0
82	1086.0
83	737.0
84	388.0
85	300.0
86	212.0
87	156.0
88	100.0
89	76.0
90	52.0
91	41.5
92	31.0
93	29.0
94	27.0
95	21.0
96	15.0
97	13.5
98	12.0
99	6.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	850401.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.177529273374432
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.87264163506379	16.604902016365006
2	8.660747925901475	3.8414798131199377
3	3.0758115511728894	2.0464170214655995
4	1.549850739926059	1.374874406162848
5	1.0010130633155365	1.1099998257355264
6	0.7296656818502099	0.9709309211405847
7	0.6160027123912506	0.9562992730574507
8	0.5026760382965252	0.8918490043476061
9	0.42929860225571703	0.8568704086690403
>10	6.482037251836213	36.14850117556761
>50	1.9054345126352612	28.13153958542502
>100	0.16682545523211362	5.4016735687531545
>500	0.006395864098355794	1.0027221515981666
>1k	0.0015989660245889486	0.6619408285924475
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2096	0.24647195852309675	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1786	0.21001856771099753	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1718	0.20202234004898867	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	982	0.11547493476606917	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	950	0.11171200410159442	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	932	0.10959535560282738	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3518316652967249E-4	0.0	0.0	0.052681029302646634	0.0
2	2.3518316652967249E-4	0.0	0.0	0.28904011166496746	0.0
3	2.3518316652967249E-4	0.0	0.0	0.37993840552868585	0.0
4	2.3518316652967249E-4	0.0	0.0	0.5082308228706223	0.0
5	2.3518316652967249E-4	0.0	0.0	0.9036913173902664	0.0
6	2.3518316652967249E-4	0.0	0.0	1.2405912034440223	0.0
7	2.3518316652967249E-4	0.0	0.0	1.5142268177012963	0.0
8	2.3518316652967249E-4	0.0	0.0	1.9501388168640441	0.0
9	2.3518316652967249E-4	0.0	0.0	2.1407547733363437	0.0
10	2.3518316652967249E-4	0.0	0.0	2.5894842550749586	0.0
11	2.3518316652967249E-4	0.0	0.0	3.164389505656743	0.0
12	2.3518316652967249E-4	0.0	0.0	3.5603203665094467	0.0
13	2.3518316652967249E-4	0.0	0.0	3.7135421995035283	0.0
14	2.3518316652967249E-4	0.0	0.0	3.7736314985518598	0.0
15	2.3518316652967249E-4	0.0	0.0	3.889929574400783	0.0
16	2.3518316652967249E-4	0.0	0.0	4.152511579831162	0.0
17	4.7036633305934497E-4	0.0	0.0	4.475770842226197	0.0
18	4.7036633305934497E-4	0.0	0.0	4.871466519912371	0.0
19	4.7036633305934497E-4	0.0	0.0	5.068550013464236	0.0
20	5.879579163241812E-4	0.0	0.0	5.257284504604298	0.0
21	5.879579163241812E-4	0.0	0.0	5.518690594202029	0.0
22	5.879579163241812E-4	0.0	0.0	5.764339411642272	0.0
23	5.879579163241812E-4	0.0	0.0	6.035388011067719	0.0
24	5.879579163241812E-4	0.0	0.0	6.265044373183945	0.0
25	5.879579163241812E-4	0.0	0.0	6.465890797400285	0.0
26	5.879579163241812E-4	0.0	0.0	6.649216075710165	0.0
27	7.055494995890174E-4	0.0	0.0	6.833364495102899	0.0
28	7.055494995890174E-4	0.0	0.0	7.0366803425678	0.0
29	7.055494995890174E-4	0.0	0.0	7.245640586029414	0.0
30	7.055494995890174E-4	0.0	0.0	7.4904662623868035	0.0
31	7.055494995890174E-4	0.0	0.0	7.7095393820091935	0.0
32	7.055494995890174E-4	0.0	0.0	7.930846741713615	0.0
33	8.231410828538537E-4	0.0	0.0	8.178377024486096	0.0
34	8.231410828538537E-4	0.0	0.0	8.399566792607253	0.0
35	9.407326661186899E-4	0.0	0.0	8.648155399629117	0.0
36	9.407326661186899E-4	0.0	0.0	8.872872915248218	0.0
37	9.407326661186899E-4	0.0	0.0	9.1097023639436	0.0
38	9.407326661186899E-4	0.0	0.0	9.34535589680633	0.0
39	0.0011759158326483624	0.0	0.0	9.577011315838057	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAG	30	2.164832E-6	45.000004	1
GTCGACG	35	1.2114288E-7	45.000004	1
TATAGCG	30	2.164832E-6	45.000004	1
CGTCCTA	20	7.032249E-4	45.000004	13
CGTTTCA	20	7.032249E-4	45.000004	44
TAGGTCG	25	3.8899474E-5	45.0	5
AATGCGC	25	3.8899474E-5	45.0	26
CGAATAT	90	0.0	45.0	14
TCGACGT	55	1.8189894E-12	45.0	26
TAGTCAA	25	3.8899474E-5	45.0	24
TAGTACG	50	2.1827873E-11	45.0	1
GTACGAG	55	1.8189894E-12	45.0	1
TGCGTAG	70	0.0	41.785717	1
TTTCGAC	60	3.6379788E-12	41.250004	24
TACGCAT	55	6.002665E-11	40.909092	16
CTACGAA	100	0.0	40.5	11
CTTACGG	50	1.0804797E-9	40.5	2
TCGTTCA	120	0.0	39.375004	16
GTATACG	40	3.4568075E-7	39.375004	1
TCGTGTA	340	0.0	39.04412	17
>>END_MODULE