SRR3553643_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553643_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	516872
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1530	0.2960113916017892	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCG	1245	0.24087201473478925	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1122	0.21707502050797878	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	902	0.1745112909966104	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	864	0.1671593740810104	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTC	840	0.16251605813431566	RNA PCR Primer, Index 46 (95% over 23bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	769	0.1487795817920104	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGC	697	0.1348496339519262	No Hit
GCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC	665	0.1286585460229999	RNA PCR Primer, Index 40 (95% over 23bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	533	0.10312030831617885	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATAC	20	7.029681E-4	45.0	28
ACCGCAC	20	7.029681E-4	45.0	12
CGTGACA	20	7.029681E-4	45.0	22
TGAACGT	25	3.8878163E-5	45.0	19
GCGTTTA	20	7.029681E-4	45.0	32
CACTGCG	50	2.1827873E-11	45.0	1
CATAGGT	20	7.029681E-4	45.0	40
CGGGCTA	20	7.029681E-4	45.0	6
CGTAATG	20	7.029681E-4	45.0	2
TGTAACG	20	7.029681E-4	45.0	1
AGTACGG	35	1.2101918E-7	45.0	2
TCGCGGA	20	7.029681E-4	45.0	23
TAGTAAG	40	6.8030204E-9	45.0	1
GTACGAG	50	2.1827873E-11	45.0	1
GTGCCGT	20	7.029681E-4	45.0	43
ACGCGCT	20	7.029681E-4	45.0	15
TACGCAT	135	0.0	43.333336	16
ACACGAC	320	0.0	42.890625	26
CACGACC	325	0.0	42.23077	27
ACGCATT	140	0.0	41.785713	17