##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553643_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 516872 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.9374738813478 33.0 31.0 34.0 30.0 34.0 2 32.042374127443544 33.0 31.0 34.0 30.0 34.0 3 32.046355770867834 33.0 31.0 34.0 30.0 34.0 4 35.70633735238125 37.0 35.0 37.0 33.0 37.0 5 35.595367905400174 37.0 35.0 37.0 33.0 37.0 6 35.553227878468945 37.0 35.0 37.0 33.0 37.0 7 35.81232297357953 37.0 35.0 37.0 35.0 37.0 8 35.7311500719714 37.0 35.0 37.0 35.0 37.0 9 37.432420405825816 39.0 37.0 39.0 35.0 39.0 10 36.905665232397965 39.0 37.0 39.0 32.0 39.0 11 36.85444365336099 39.0 37.0 39.0 33.0 39.0 12 36.752793728427925 39.0 35.0 39.0 33.0 39.0 13 36.69088478385364 39.0 35.0 39.0 33.0 39.0 14 37.774547663638195 40.0 36.0 41.0 33.0 41.0 15 37.906030893528765 40.0 37.0 41.0 33.0 41.0 16 37.95334821774056 40.0 36.0 41.0 33.0 41.0 17 37.87039731306784 40.0 36.0 41.0 33.0 41.0 18 37.84656162454147 40.0 36.0 41.0 33.0 41.0 19 37.751091179247474 40.0 36.0 41.0 33.0 41.0 20 37.67972728257673 40.0 36.0 41.0 33.0 41.0 21 37.54821116253154 39.0 35.0 41.0 33.0 41.0 22 37.56875009673575 39.0 35.0 41.0 33.0 41.0 23 37.46491974802272 39.0 35.0 41.0 33.0 41.0 24 37.42074633564983 39.0 35.0 41.0 32.0 41.0 25 37.268190190221176 39.0 35.0 41.0 32.0 41.0 26 37.210922626878606 39.0 35.0 41.0 32.0 41.0 27 37.20900338962064 39.0 35.0 41.0 32.0 41.0 28 37.10834984290114 39.0 35.0 41.0 32.0 41.0 29 37.08214219381201 39.0 35.0 41.0 32.0 41.0 30 36.91666989119163 39.0 35.0 41.0 31.0 41.0 31 36.865653391942296 39.0 35.0 41.0 31.0 41.0 32 36.68255583587426 39.0 35.0 41.0 31.0 41.0 33 36.57204878577288 39.0 35.0 41.0 31.0 41.0 34 36.453021250909316 39.0 35.0 41.0 30.0 41.0 35 36.366179634416255 39.0 35.0 41.0 30.0 41.0 36 36.198256047919024 39.0 35.0 41.0 30.0 41.0 37 36.19604660341439 39.0 35.0 41.0 30.0 41.0 38 36.04048391091025 38.0 35.0 40.0 29.0 41.0 39 35.91815768700955 38.0 35.0 40.0 29.0 41.0 40 35.788916791778234 38.0 35.0 40.0 29.0 41.0 41 35.68398365552787 38.0 35.0 40.0 28.0 41.0 42 35.569268600349794 38.0 34.0 40.0 28.0 41.0 43 35.49874243526444 38.0 34.0 40.0 28.0 41.0 44 35.39577303471653 38.0 34.0 40.0 27.0 41.0 45 35.31703593926543 38.0 34.0 40.0 27.0 41.0 46 35.11442097850145 37.0 34.0 40.0 27.0 41.0 47 35.00992315312108 37.0 34.0 40.0 26.0 41.0 48 34.917567598941325 37.0 34.0 40.0 26.0 41.0 49 34.82595884474299 37.0 34.0 40.0 26.0 41.0 50 34.7114024361931 36.0 34.0 40.0 26.0 41.0 51 33.263999597579286 35.0 31.0 39.0 22.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 7.0 10 1.0 11 8.0 12 7.0 13 12.0 14 18.0 15 35.0 16 83.0 17 134.0 18 264.0 19 458.0 20 789.0 21 1169.0 22 1813.0 23 2302.0 24 3157.0 25 4026.0 26 5107.0 27 6140.0 28 7024.0 29 8409.0 30 10226.0 31 12993.0 32 16819.0 33 22667.0 34 37998.0 35 46716.0 36 45078.0 37 62276.0 38 96938.0 39 124164.0 40 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.19837019610271 17.583657075639618 23.178659319908988 15.039313408348681 2 29.639446516739156 26.748595396926124 25.13059326100079 18.48136482533393 3 32.34069556872882 25.76982308966243 24.913711711990587 16.975769629618163 4 28.119727901685525 25.91705489947221 26.198942871736136 19.76427432710613 5 25.414415948242503 29.94377718274544 24.464470894147876 20.17733597486418 6 24.503552136699223 36.91339441873423 23.73992013496572 14.843133309600828 7 80.6683279419276 6.939048739339721 8.0677614573821 4.324861861350586 8 83.41407543840641 6.304268755126994 6.620981596991131 3.66067420947546 9 78.24916033369963 7.699778668606541 8.39995201906855 5.651108978625269 10 43.091326285811576 25.790331068426998 15.891168413069387 15.22717423269204 11 32.16173443328329 26.18926929684719 21.45482827469857 20.19416799517095 12 28.512668513674566 23.09604699035738 27.867247597084 20.524036898884056 13 25.225200823414696 25.55294154065223 28.30043028061106 20.921427355322013 14 21.20138061260815 27.59832221517126 28.04504790354285 23.15524926867774 15 20.352040737358575 26.295098206132273 31.290725750282466 22.062135306226686 16 23.718251327214475 23.344851336501108 30.768739649274867 22.168157687009547 17 23.532712160844465 24.60318995805538 26.69036047609466 25.173737405005493 18 23.924298472349054 24.393660325960777 28.020283551827145 23.66175764986302 19 24.83496881239456 27.032998498661176 25.084740516027175 23.047292172917086 20 26.521459858533643 24.879273785385937 25.686049931124145 22.913216424956275 21 25.693014905044187 26.786322338993017 25.39197325450015 22.128689501462645 22 24.44183472890774 24.405655558823074 24.39888405640081 26.75362565586838 23 23.756945626770264 28.209305205157175 25.17876766394775 22.85498150412481 24 23.73740500549459 25.788976767942547 28.496803850856693 21.976814375706173 25 24.701280007429304 25.83250785494281 26.374808463217196 23.091403674410685 26 21.9342506461948 26.67449581327679 26.77877695057964 24.612476589948766 27 22.238194369205527 26.20087758671393 27.54298936680648 24.017938677274064 28 21.201187141110374 26.428400068101965 29.13874228048724 23.23167051030042 29 22.464942964602454 25.32077574331749 26.68900617561021 25.525275116469842 30 22.73038585955517 26.841268244362244 26.197782042749463 24.230563853333127 31 24.820458450061135 26.414663591759663 24.08874924546116 24.676128712718043 32 24.879467256883718 26.98656533919423 25.258477921032675 22.87548948288938 33 24.54398767973502 26.536163692364838 23.59752511260041 25.32232351529973 34 22.6216548778034 27.114449999226114 25.124402173071864 25.139492949898624 35 23.080182327539507 25.50012382175858 26.305545667012336 25.11414818368958 36 24.290540017644602 28.41941525174511 24.269064681391136 23.02098004921915 37 23.31660449782538 27.525770403504158 25.77543376309802 23.382191335572443 38 23.4891810738442 28.402196288442788 25.918215728458883 22.19040690925413 39 24.305824265969136 26.529972604435915 25.581962265319074 23.582240864275875 40 23.516073612035477 26.420274265195253 27.127412589577304 22.936239533191973 41 21.36892692968472 26.152316240771412 25.90602702409881 26.57272980544506 42 22.05729851878221 26.82637093903326 25.520438329025367 25.595892213159154 43 22.672731353217042 26.150961940286955 24.97562259127985 26.200684115216145 44 22.443467628348994 26.12445634509124 26.030235725672895 25.401840300886875 45 23.99085266758501 26.053258833908586 25.20256465817456 24.75332384033184 46 23.848844588215265 27.72465910322091 25.260606107508245 23.165890201055582 47 22.383684935535296 26.0623519943042 28.998668916095284 22.555294154065223 48 23.396895169403646 25.313423826401895 27.213313934591156 24.076367069603304 49 22.674666068194835 25.89364484824096 28.12591898961445 23.30577009394976 50 22.356405454348465 25.459494807224996 27.922386973950996 24.261712764475536 51 23.461127706666254 25.313423826401895 25.355987555913263 25.86946091101859 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1984.0 1 2221.0 2 2458.0 3 2535.0 4 2612.0 5 1892.0 6 1172.0 7 1189.5 8 1207.0 9 1288.5 10 1370.0 11 1374.5 12 1379.0 13 1358.5 14 1338.0 15 1286.5 16 1235.0 17 1233.0 18 1231.0 19 1275.0 20 1319.0 21 1593.0 22 1867.0 23 1873.0 24 1879.0 25 2355.0 26 3291.5 27 3752.0 28 4624.0 29 5496.0 30 7075.5 31 8655.0 32 9465.5 33 10276.0 34 11101.5 35 11927.0 36 13092.5 37 14258.0 38 14875.0 39 15492.0 40 17394.0 41 19296.0 42 22340.5 43 25385.0 44 26780.5 45 28176.0 46 32574.5 47 36973.0 48 36402.0 49 35831.0 50 37362.0 51 38893.0 52 37420.0 53 35947.0 54 34538.5 55 33130.0 56 30950.0 57 28770.0 58 27907.5 59 27045.0 60 26185.5 61 25326.0 62 23247.0 63 21168.0 64 20020.0 65 18872.0 66 16250.5 67 13629.0 68 12517.5 69 11406.0 70 9313.0 71 7220.0 72 6685.0 73 6150.0 74 5086.0 75 3444.5 76 2867.0 77 2163.5 78 1460.0 79 1094.5 80 729.0 81 649.0 82 569.0 83 364.5 84 160.0 85 99.0 86 38.0 87 30.5 88 23.0 89 22.0 90 21.0 91 23.0 92 25.0 93 13.0 94 1.0 95 1.5 96 2.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 516872.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.557914265673357 #Duplication Level Percentage of deduplicated Percentage of total 1 76.04692736295581 17.91506994984402 2 8.070521334780862 3.802492993681106 3 2.778669315657782 1.9637896053276986 4 1.5281772231495077 1.4400267202284354 5 0.9700350883067029 1.1426001722512096 6 0.7350076242555529 1.0389147958097145 7 0.611481669722199 1.008366292524245 8 0.5225862465255279 0.9848833593654741 9 0.46250689528005073 0.980612800766118 >10 6.461096011844352 38.02514972987445 >50 1.6961302405456944 26.058512640027292 >100 0.10780837530882385 3.764244337614537 >500 0.006583717576111381 1.1198122833777076 >1k 0.0024688940910417676 0.7555243193079857 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1530 0.2960113916017892 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCG 1245 0.24087201473478925 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1122 0.21707502050797878 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 902 0.1745112909966104 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 864 0.1671593740810104 No Hit GAATCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTC 840 0.16251605813431566 RNA PCR Primer, Index 46 (95% over 23bp) ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC 769 0.1487795817920104 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGC 697 0.1348496339519262 No Hit GCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC 665 0.1286585460229999 RNA PCR Primer, Index 40 (95% over 23bp) GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT 533 0.10312030831617885 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.934714977789472E-4 0.0 0.0 0.04430497299137891 0.0 2 1.934714977789472E-4 0.0 0.0 0.3082000959618629 0.0 3 1.934714977789472E-4 0.0 0.0 0.39371449798015756 0.0 4 1.934714977789472E-4 0.0 0.0 0.5419136652788311 0.0 5 1.934714977789472E-4 0.0 0.0 1.1025940658422202 0.0 6 1.934714977789472E-4 0.0 0.0 1.4978563358046093 0.0 7 1.934714977789472E-4 0.0 0.0 1.8457180888111564 0.0 8 1.934714977789472E-4 0.0 0.0 2.352613412991998 0.0 9 1.934714977789472E-4 0.0 0.0 2.576072992926682 0.0 10 1.934714977789472E-4 0.0 0.0 3.253610178148555 0.0 11 1.934714977789472E-4 0.0 0.0 3.8123558637341546 0.0 12 1.934714977789472E-4 0.0 0.0 4.394511600551007 0.0 13 1.934714977789472E-4 0.0 0.0 4.578696466436565 0.0 14 1.934714977789472E-4 0.0 0.0 4.648539677134765 0.0 15 1.934714977789472E-4 0.0 0.0 4.7909346995000694 0.0 16 1.934714977789472E-4 0.0 0.0 5.05908619542169 0.0 17 3.869429955578944E-4 0.0 0.0 5.397080902041512 0.0 18 3.869429955578944E-4 0.0 0.0 5.812270736275132 0.0 19 3.869429955578944E-4 0.0 0.0 6.037278088192047 0.0 20 3.869429955578944E-4 0.0 0.0 6.2259127985265215 0.0 21 3.869429955578944E-4 0.0 0.0 6.463108854803511 0.0 22 3.869429955578944E-4 0.0 0.0 6.7223606618273 0.0 23 3.869429955578944E-4 0.0 0.0 6.967489049513226 0.0 24 5.804144933368417E-4 0.0 0.0 7.175083966630036 0.0 25 5.804144933368417E-4 0.0 0.0 7.362944790973394 0.0 26 5.804144933368417E-4 0.0 0.0 7.550418672321194 0.0 27 5.804144933368417E-4 0.0 0.0 7.7334427092200775 0.0 28 5.804144933368417E-4 0.0 0.0 7.919368818585646 0.0 29 5.804144933368417E-4 0.0 0.0 8.104521041960098 0.0 30 5.804144933368417E-4 0.0 0.0 8.328174093392562 0.0 31 7.738859911157888E-4 0.0 0.0 8.526095435620425 0.0 32 7.738859911157888E-4 0.0 0.0 8.740848798155056 0.0 33 7.738859911157888E-4 0.0 0.0 8.959278119147488 0.0 34 7.738859911157888E-4 0.0 0.0 9.176740082651024 0.0 35 7.738859911157888E-4 0.0 0.0 9.431542045225898 0.0 36 7.738859911157888E-4 0.0 0.0 9.633913231902676 0.0 37 7.738859911157888E-4 0.0 0.0 9.852729495890665 0.0 38 7.738859911157888E-4 0.0 0.0 10.065548143447508 0.0 39 7.738859911157888E-4 0.0 0.0 10.287846894395518 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTATAC 20 7.029681E-4 45.0 28 ACCGCAC 20 7.029681E-4 45.0 12 CGTGACA 20 7.029681E-4 45.0 22 TGAACGT 25 3.8878163E-5 45.0 19 GCGTTTA 20 7.029681E-4 45.0 32 CACTGCG 50 2.1827873E-11 45.0 1 CATAGGT 20 7.029681E-4 45.0 40 CGGGCTA 20 7.029681E-4 45.0 6 CGTAATG 20 7.029681E-4 45.0 2 TGTAACG 20 7.029681E-4 45.0 1 AGTACGG 35 1.2101918E-7 45.0 2 TCGCGGA 20 7.029681E-4 45.0 23 TAGTAAG 40 6.8030204E-9 45.0 1 GTACGAG 50 2.1827873E-11 45.0 1 GTGCCGT 20 7.029681E-4 45.0 43 ACGCGCT 20 7.029681E-4 45.0 15 TACGCAT 135 0.0 43.333336 16 ACACGAC 320 0.0 42.890625 26 CACGACC 325 0.0 42.23077 27 ACGCATT 140 0.0 41.785713 17 >>END_MODULE