FastQCFastQC Report
Sat 18 Jun 2016
SRR3553642_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553642_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509240
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30430.5975571439792632No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCG18610.36544654779671665No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16800.329903385437122No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15680.3079098264079805No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGC12990.25508601052548896No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTC12880.2529259288351268No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCC11160.2191501060403739No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC8600.16887911397376484No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7930.155722252768832No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA7000.13745974393213417No Hit
GCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC6260.12292828528787998No Hit
CCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC6060.11900086403267615No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5200.10211295263529965No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGTA453.8380676E-1045.00000410
TTGATCG207.0295826E-445.024
TCGCAAT207.0295826E-445.041
GATCGTA207.0295826E-445.010
CAACGAC253.887734E-545.016
GTCCTCG207.0295826E-445.038
GACGTAG207.0295826E-445.01
GTTACCG207.0295826E-445.043
TACGCGG351.2101373E-745.02
GTTAACG207.0295826E-445.01
CATAGCG207.0295826E-445.01
CTACGCA1050.045.015
CGAGGTT253.887734E-545.014
CGCTAGG207.0295826E-445.02
GTCAATT351.2101373E-745.015
GTACTAG351.2101373E-745.01
TCGTACT253.887734E-545.012
TACTCGC207.0295826E-445.045
TCATCGG207.0295826E-445.02
ATGCGTT207.0295826E-445.012