##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553642_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	509240
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.901545440263924	33.0	31.0	34.0	30.0	34.0
2	32.0188457308931	33.0	31.0	34.0	30.0	34.0
3	31.974512999764354	33.0	31.0	34.0	30.0	34.0
4	35.66560364464692	37.0	35.0	37.0	33.0	37.0
5	35.57239219228654	37.0	35.0	37.0	33.0	37.0
6	35.539071950357396	37.0	35.0	37.0	33.0	37.0
7	35.796606708035505	37.0	35.0	37.0	35.0	37.0
8	35.67354489042495	37.0	35.0	37.0	35.0	37.0
9	37.40665305160631	39.0	37.0	39.0	35.0	39.0
10	36.913203990259994	39.0	37.0	39.0	33.0	39.0
11	36.82920626816432	39.0	37.0	39.0	33.0	39.0
12	36.71718050428089	39.0	35.0	39.0	33.0	39.0
13	36.63972193857513	39.0	35.0	39.0	32.0	39.0
14	37.728418820202656	40.0	36.0	41.0	33.0	41.0
15	37.887123949414814	40.0	36.0	41.0	33.0	41.0
16	37.94387125913126	40.0	36.0	41.0	33.0	41.0
17	37.85083654072736	40.0	36.0	41.0	33.0	41.0
18	37.789678736941326	39.0	36.0	41.0	33.0	41.0
19	37.670181054119865	39.0	36.0	41.0	33.0	41.0
20	37.5519067630194	39.0	35.0	41.0	33.0	41.0
21	37.43872240986568	39.0	35.0	41.0	33.0	41.0
22	37.43562956562721	39.0	35.0	41.0	33.0	41.0
23	37.32936532872516	39.0	35.0	41.0	32.0	41.0
24	37.299245935119	39.0	35.0	41.0	32.0	41.0
25	37.167127484093946	39.0	35.0	41.0	32.0	41.0
26	37.11447647474668	39.0	35.0	41.0	32.0	41.0
27	37.063255046736316	39.0	35.0	41.0	32.0	41.0
28	36.99428560207368	39.0	35.0	41.0	31.0	41.0
29	36.95721467284581	39.0	35.0	41.0	32.0	41.0
30	36.79812662006127	39.0	35.0	41.0	31.0	41.0
31	36.655040845181055	39.0	35.0	41.0	31.0	41.0
32	36.45343256617705	39.0	35.0	41.0	30.0	41.0
33	36.30338740083261	39.0	35.0	41.0	30.0	41.0
34	36.154308381116955	39.0	35.0	41.0	30.0	41.0
35	36.027209174456054	39.0	35.0	41.0	29.0	41.0
36	35.883059068415676	38.0	35.0	40.0	29.0	41.0
37	35.83838268792711	38.0	35.0	40.0	28.0	41.0
38	35.71560560835755	38.0	35.0	40.0	28.0	41.0
39	35.62336030162595	38.0	35.0	40.0	27.0	41.0
40	35.47136713533894	38.0	35.0	40.0	27.0	41.0
41	35.33874793810384	38.0	34.0	40.0	26.0	41.0
42	35.222227240593824	38.0	34.0	40.0	26.0	41.0
43	35.14995090723431	38.0	34.0	40.0	26.0	41.0
44	35.04798523289608	38.0	34.0	40.0	26.0	41.0
45	35.02118254653994	38.0	34.0	40.0	26.0	41.0
46	34.8100384887283	37.0	34.0	40.0	25.0	41.0
47	34.69807556358495	37.0	34.0	40.0	24.0	41.0
48	34.600206189615896	37.0	34.0	40.0	24.0	41.0
49	34.49015984604509	37.0	34.0	40.0	24.0	41.0
50	34.335637420469716	36.0	33.0	40.0	24.0	41.0
51	32.88937436179405	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	6.0
12	5.0
13	10.0
14	9.0
15	43.0
16	69.0
17	147.0
18	259.0
19	495.0
20	892.0
21	1510.0
22	2270.0
23	3020.0
24	3820.0
25	4966.0
26	5878.0
27	6828.0
28	7671.0
29	8776.0
30	10523.0
31	12962.0
32	16595.0
33	22592.0
34	37805.0
35	47176.0
36	42013.0
37	59404.0
38	91790.0
39	121674.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.288586913832376	19.724491398947453	22.750962218207523	16.235959469012645
2	30.140798051999056	28.08400754064881	24.919487864268323	16.855706543083812
3	31.01680936297227	27.820477574424636	25.489356688398395	15.673356374204698
4	27.603683921137378	26.860615819652818	26.52894509465085	19.00675516455895
5	25.422197784934415	30.679640248213026	24.442109810698295	19.456052156154268
6	23.447097635692405	37.73564527531223	24.20960647239023	14.607650616605136
7	78.03412929070772	8.150184588798995	10.056358495012176	3.759327625481109
8	79.76474746681329	8.62677715811798	7.907862697352918	3.7006126777158115
9	74.55070300840468	9.023250333830807	9.861165658628543	6.564880999135967
10	41.223980834184275	26.458055140994425	17.339957583850442	14.978006440970859
11	31.596103998114838	26.67347419684235	23.481266200612676	18.249155604430133
12	28.24051527766868	23.73144293456916	28.90503495404917	19.12300683371298
13	24.342156939753355	25.901932291257562	29.58820988139188	20.167700887597203
14	19.377700102112954	30.477378053570025	28.55569083339879	21.58923101091823
15	19.297580708506796	26.634396355353076	33.47969523211059	20.588327704029535
16	21.07670253711413	25.33815097007305	32.188162752336815	21.396983740476003
17	21.170764276176264	25.42278689812269	28.643468698452594	24.76298012724845
18	22.41045479538135	26.09300133532323	28.618333202419294	22.87821066687613
19	24.44053884219621	27.71836462178933	26.234584871573325	21.60651166444113
20	25.605608357552427	26.417799073128585	26.058440028277435	21.918152541041554
21	23.25799230225434	28.55353075170843	26.979223941559972	21.20925300447726
22	21.95801586678187	26.01248919959155	27.08879899458016	24.940695939046424
23	22.337012017909043	29.346869845259604	26.036250098185533	22.279868038645827
24	22.056005027099207	27.873105019244367	28.375422197784932	21.695467755871494
25	21.466499096693113	28.574738826486527	27.543987118058283	22.414774958762077
26	20.425928835126854	29.10886811719425	28.001335323226765	22.463867724452125
27	22.07996229675595	28.182782185217185	27.37510800408452	22.362147513942347
28	20.47070143743618	28.424711334537744	29.240043987118057	21.86454324090802
29	21.92266907548504	27.555769381823897	27.579137538292358	22.942424004398713
30	22.517869766711176	27.597203676066297	26.883002120807475	23.00192443641505
31	24.155211688005657	28.495797659256933	24.62120021993559	22.72779043280182
32	24.37554002042259	30.112913361087113	24.521247348990652	20.990299269499644
33	24.082750765847145	28.80194014610007	23.537035582436573	23.578273505616213
34	22.90668447097636	28.081847458958446	26.11774408923101	22.893723980834185
35	22.628819417170686	27.326604351582752	25.115662555965752	24.92891367528081
36	23.514649281281912	29.951496347498235	25.355038881470428	21.178815489749432
37	22.92219778493441	28.4017359201948	27.299701515984605	21.376364778886185
38	22.07623124656351	29.679129683449844	25.189694446626344	23.054944623360303
39	23.049838975728537	28.645628780142957	26.264040530987355	22.04049171314115
40	23.009582907862697	26.679758070850678	28.035111146021524	22.2755478752651
41	20.293181996700966	27.222724059382607	26.847655329510644	25.636438614405783
42	21.556044301311758	28.480284345298877	25.903896001885162	24.059775351504204
43	22.143979263215773	26.997093708271148	26.04646139344906	24.81246563506402
44	21.64912418506009	27.902364307595633	26.617704815018463	23.83080669232582
45	22.497839918309637	27.9074699552274	25.936493598303358	23.65819652815961
46	22.12257481737491	28.823933705129214	26.510682585814155	22.54280889168172
47	21.555062445997958	27.466616919330768	27.980323619511427	22.997997015159847
48	22.55144921844317	26.825661770481503	27.327586206896555	23.295302804178778
49	22.293810384101796	26.552313251119315	28.609496504595082	22.544379860183803
50	21.21121671510486	27.47113345377425	27.803589663027257	23.51406016809363
51	21.674456052156156	27.252376089859396	25.74954834655565	25.323619511428795
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3646.0
1	3812.0
2	3978.0
3	3904.0
4	3830.0
5	2798.0
6	1766.0
7	1743.5
8	1721.0
9	1846.0
10	1971.0
11	1940.5
12	1910.0
13	1984.0
14	2058.0
15	2043.5
16	2029.0
17	2072.5
18	2116.0
19	2173.5
20	2231.0
21	2264.5
22	2298.0
23	2867.5
24	3437.0
25	3751.0
26	4655.0
27	5245.0
28	6446.5
29	7648.0
30	8787.5
31	9927.0
32	10451.0
33	10975.0
34	11584.5
35	12194.0
36	13046.0
37	13898.0
38	15890.5
39	17883.0
40	19505.5
41	21128.0
42	23464.5
43	25801.0
44	28107.5
45	30414.0
46	35786.0
47	41158.0
48	38936.5
49	36715.0
50	37223.0
51	37731.0
52	35406.5
53	33082.0
54	31266.0
55	29450.0
56	27137.0
57	24824.0
58	22908.0
59	20992.0
60	20953.5
61	20915.0
62	19732.5
63	18550.0
64	16299.5
65	14049.0
66	12674.5
67	11300.0
68	10165.0
69	9030.0
70	7421.0
71	5812.0
72	5613.0
73	5414.0
74	4584.5
75	2712.0
76	1669.0
77	1566.5
78	1464.0
79	975.0
80	486.0
81	403.5
82	321.0
83	248.5
84	176.0
85	139.0
86	102.0
87	71.0
88	40.0
89	25.0
90	10.0
91	7.0
92	4.0
93	5.5
94	7.0
95	5.0
96	3.0
97	5.5
98	8.0
99	6.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	509240.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.59662068817102
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.5289739050605	19.588831167027713
2	8.74302476348258	4.475837770762995
3	3.1819515101687568	2.4434161756182786
4	1.5722694817858949	1.6097914217944307
5	1.0040532170346987	1.2850184673587517
6	0.7101833710282788	1.0906976620354485
7	0.5291394560151316	0.9480927362735134
8	0.41064688816442674	0.8408938106498093
9	0.3506519706080387	0.8077954936692291
>10	5.4504052692097416	34.76598690107994
>50	1.3464526547802134	22.86515164549692
>100	0.16225100523051117	6.13710684789782
>500	0.004613772660583256	0.8079802312578753
>1k	0.005382734770680465	2.33339966907725
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3043	0.5975571439792632	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCG	1861	0.36544654779671665	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1680	0.329903385437122	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1568	0.3079098264079805	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGC	1299	0.25508601052548896	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTC	1288	0.2529259288351268	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCC	1116	0.2191501060403739	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	860	0.16887911397376484	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	793	0.155722252768832	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	700	0.13745974393213417	No Hit
GCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	626	0.12292828528787998	No Hit
CCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	606	0.11900086403267615	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	520	0.10211295263529965	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05753672138873615	0.0
2	0.0	0.0	0.0	0.3882255910768989	0.0
3	0.0	0.0	0.0	0.5445369570340115	0.0
4	0.0	0.0	0.0	0.8100306338857906	0.0
5	0.0	0.0	0.0	1.6779907312858378	0.0
6	0.0	0.0	0.0	2.1840389600188517	0.0
7	0.0	0.0	0.0	2.6749666169193307	0.0
8	0.0	0.0	0.0	3.3390935511742987	0.0
9	0.0	0.0	0.0	3.6165658628544497	0.0
10	1.9637106276019166E-4	0.0	0.0	4.592333673709842	0.0
11	1.9637106276019166E-4	0.0	0.0	5.534718403896002	0.0
12	1.9637106276019166E-4	0.0	0.0	6.433116016023878	0.0
13	1.9637106276019166E-4	0.0	0.0	6.68054355510172	0.0
14	1.9637106276019166E-4	0.0	0.0	6.788351268557065	0.0
15	1.9637106276019166E-4	0.0	0.0	6.988453381509701	0.0
16	1.9637106276019166E-4	0.0	0.0	7.386301154661849	0.0
17	1.9637106276019166E-4	0.0	0.0	7.862697352918074	0.0
18	1.9637106276019166E-4	0.0	0.0	8.42137302647082	0.0
19	1.9637106276019166E-4	0.0	0.0	8.75913125441835	0.0
20	1.9637106276019166E-4	0.0	0.0	9.05741889875108	0.0
21	1.9637106276019166E-4	0.0	0.0	9.393998900322048	0.0
22	3.9274212552038333E-4	0.0	0.0	9.724687770010211	0.0
23	3.9274212552038333E-4	0.0	0.0	10.090527059932448	0.0
24	3.9274212552038333E-4	0.0	0.0	10.360930013353233	0.0
25	3.9274212552038333E-4	0.0	0.0	10.60521561542691	0.0
26	3.9274212552038333E-4	0.0	0.0	10.841646374990182	0.0
27	3.9274212552038333E-4	0.0	0.0	11.081808184745896	0.0
28	3.9274212552038333E-4	0.0	0.0	11.333555887204461	0.0
29	3.9274212552038333E-4	0.0	0.0	11.610242714633571	0.0
30	3.9274212552038333E-4	0.0	0.0	11.923847301861597	0.0
31	3.9274212552038333E-4	0.0	0.0	12.203872437357631	0.0
32	3.9274212552038333E-4	0.0	0.0	12.492930641740633	0.0
33	3.9274212552038333E-4	0.0	0.0	12.759406173906212	0.0
34	5.89113188280575E-4	0.0	0.0	13.039627680465006	0.0
35	5.89113188280575E-4	0.0	0.0	13.343610085617783	0.0
36	7.854842510407667E-4	0.0	0.0	13.635221113816668	0.0
37	9.818553138009583E-4	0.0	0.0	13.935865210902522	0.0
38	0.00117822637656115	0.0	0.0	14.231010918231089	0.0
39	0.00117822637656115	0.0	0.0	14.52006912261409	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATGTA	45	3.8380676E-10	45.000004	10
TTGATCG	20	7.0295826E-4	45.0	24
TCGCAAT	20	7.0295826E-4	45.0	41
GATCGTA	20	7.0295826E-4	45.0	10
CAACGAC	25	3.887734E-5	45.0	16
GTCCTCG	20	7.0295826E-4	45.0	38
GACGTAG	20	7.0295826E-4	45.0	1
GTTACCG	20	7.0295826E-4	45.0	43
TACGCGG	35	1.2101373E-7	45.0	2
GTTAACG	20	7.0295826E-4	45.0	1
CATAGCG	20	7.0295826E-4	45.0	1
CTACGCA	105	0.0	45.0	15
CGAGGTT	25	3.887734E-5	45.0	14
CGCTAGG	20	7.0295826E-4	45.0	2
GTCAATT	35	1.2101373E-7	45.0	15
GTACTAG	35	1.2101373E-7	45.0	1
TCGTACT	25	3.887734E-5	45.0	12
TACTCGC	20	7.0295826E-4	45.0	45
TCATCGG	20	7.0295826E-4	45.0	2
ATGCGTT	20	7.0295826E-4	45.0	12
>>END_MODULE