SRR3553639_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553639_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	538898
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2208	0.4097250314530764	No Hit
CCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	2068	0.38374608924137776	TruSeq Adapter, Index 23 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	1778	0.32993256608857335	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGCT	1291	0.23956295996645005	TruSeq Adapter, Index 20 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1196	0.22193439203708307	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1086	0.20152236601360557	No Hit
TCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	807	0.14975004546314885	TruSeq Adapter, Index 23 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	784	0.14548207638551267	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCC	765	0.14195636279963927	No Hit
AAAACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC	726	0.13471937175495177	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	653	0.12117320903028031	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	619	0.11486403735029634	No Hit
ACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	604	0.11208057925618577	TruSeq Adapter, Index 20 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	553	0.10261682173620983	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCA	30	2.1633477E-6	45.000004	6
TCGCACG	25	3.888037E-5	45.000004	1
TAGACTA	25	3.888037E-5	45.000004	35
TCGATAG	25	3.888037E-5	45.000004	43
ATGTACG	30	2.1633477E-6	45.000004	1
CACTCGC	25	3.888037E-5	45.000004	28
TTACGTT	25	3.888037E-5	45.000004	34
GCTACGA	100	0.0	45.000004	10
AATAGCG	30	2.1633477E-6	45.000004	45
CCTATCA	25	3.888037E-5	45.000004	21
GCTCGAT	25	3.888037E-5	45.000004	41
GTTGGCG	35	1.2103192E-7	45.000004	1
CAATACG	30	2.1633477E-6	45.000004	1
CGATAGC	30	2.1633477E-6	45.000004	10
ACGAACT	25	3.888037E-5	45.000004	1
AACACGA	35	1.2103192E-7	45.000004	45
ACAACGA	90	0.0	45.0	13
TTCGTTA	20	7.029949E-4	45.0	1
TGACGAT	20	7.029949E-4	45.0	37
ACGGGTA	105	0.0	45.0	5