##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553639_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	538898
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.833304261659904	33.0	31.0	34.0	30.0	34.0
2	31.931764452642245	33.0	31.0	34.0	30.0	34.0
3	31.91734242843729	33.0	31.0	34.0	30.0	34.0
4	35.60249991649626	37.0	35.0	37.0	33.0	37.0
5	35.51449068283794	37.0	35.0	37.0	33.0	37.0
6	35.48064939932974	37.0	35.0	37.0	33.0	37.0
7	35.79068395132288	37.0	35.0	37.0	35.0	37.0
8	35.715712435377384	37.0	35.0	37.0	35.0	37.0
9	37.4279251361111	39.0	37.0	39.0	35.0	39.0
10	36.86958385445854	39.0	37.0	39.0	32.0	39.0
11	36.83971363783128	39.0	37.0	39.0	33.0	39.0
12	36.75067266903941	39.0	35.0	39.0	33.0	39.0
13	36.676079703394706	39.0	35.0	39.0	33.0	39.0
14	37.72516691470371	40.0	36.0	41.0	33.0	41.0
15	37.88523987841855	40.0	36.0	41.0	33.0	41.0
16	37.916334816607225	40.0	36.0	41.0	33.0	41.0
17	37.87819958507918	40.0	36.0	41.0	33.0	41.0
18	37.85390370719505	39.0	36.0	41.0	33.0	41.0
19	37.77871137024075	39.0	36.0	41.0	33.0	41.0
20	37.72620421675345	39.0	36.0	41.0	33.0	41.0
21	37.577526730475896	39.0	35.0	41.0	33.0	41.0
22	37.558601071074676	39.0	35.0	41.0	33.0	41.0
23	37.504058281901216	39.0	35.0	41.0	33.0	41.0
24	37.47455177046491	39.0	35.0	41.0	33.0	41.0
25	37.359440933163604	39.0	35.0	41.0	32.0	41.0
26	37.327067459890365	39.0	35.0	41.0	32.0	41.0
27	37.28886171409061	39.0	35.0	41.0	32.0	41.0
28	37.168766631162114	39.0	35.0	41.0	32.0	41.0
29	37.162429624901186	39.0	35.0	41.0	32.0	41.0
30	37.00881799524214	39.0	35.0	41.0	32.0	41.0
31	36.92845770442644	39.0	35.0	41.0	31.0	41.0
32	36.810086509877564	39.0	35.0	41.0	31.0	41.0
33	36.75074318331113	39.0	35.0	41.0	31.0	41.0
34	36.64198419738058	39.0	35.0	41.0	31.0	41.0
35	36.61459682537326	39.0	35.0	41.0	31.0	41.0
36	36.44659286172894	39.0	35.0	41.0	30.0	41.0
37	36.392139143214486	39.0	35.0	40.0	30.0	41.0
38	36.27416876663116	39.0	35.0	40.0	30.0	41.0
39	36.225523197339754	39.0	35.0	40.0	30.0	41.0
40	36.10838785818466	38.0	35.0	40.0	30.0	41.0
41	35.99518090621973	38.0	35.0	40.0	29.0	41.0
42	35.90086435652016	38.0	35.0	40.0	29.0	41.0
43	35.7776165433904	38.0	35.0	40.0	29.0	41.0
44	35.67369335198869	38.0	35.0	40.0	29.0	41.0
45	35.62485108499197	38.0	35.0	40.0	29.0	41.0
46	35.48485242105185	38.0	34.0	40.0	28.0	41.0
47	35.38928702648739	38.0	34.0	40.0	28.0	41.0
48	35.32147270912121	38.0	34.0	40.0	28.0	41.0
49	35.258900571165604	37.0	34.0	40.0	27.0	41.0
50	35.127447123574406	37.0	34.0	40.0	27.0	41.0
51	33.669828056515335	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	6.0
13	12.0
14	11.0
15	36.0
16	51.0
17	94.0
18	227.0
19	435.0
20	736.0
21	1155.0
22	1656.0
23	2181.0
24	2874.0
25	3766.0
26	4765.0
27	5541.0
28	6670.0
29	8320.0
30	10227.0
31	12944.0
32	17112.0
33	23505.0
34	39312.0
35	49921.0
36	46209.0
37	65549.0
38	104392.0
39	131156.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.461282097910924	19.47734079547521	23.205319002853972	14.856058103759896
2	29.998626827340242	25.238913486411157	26.042219492371473	18.720240193877135
3	31.424128499270733	25.863521482729567	26.541757438327846	16.17059257967185
4	27.414649896640924	27.415021024386803	25.594082739219665	19.576246339752608
5	24.205879405750252	30.62583271787982	25.132585387216132	20.035702489153792
6	22.965941606760463	36.22856273357852	25.338932413926194	15.466563245734816
7	78.70060753612	7.733003277057997	8.672142038010904	4.894247148811092
8	80.07044004616829	6.510694045997573	8.025266376939607	5.393599530894529
9	75.17006928955016	8.472846438472587	10.199703840058786	6.157380431918471
10	39.27199581367903	26.038322651039714	18.4114619093038	16.278219625977457
11	30.608018586077513	27.067645454241806	22.91713830817706	19.407197651503623
12	26.41427505761758	23.03088896228971	30.119243344751695	20.43559263534101
13	22.858500124327794	26.254318999142694	30.46272207356494	20.42445880296457
14	20.467509621486812	29.762589580959663	28.252099655222324	21.5178011423312
15	18.75085823291235	27.177313703149764	33.75944984022951	20.312378223708382
16	19.960920248358686	24.39719575875212	33.221129044828515	22.420754948060672
17	20.270811916169666	24.712468778878378	29.28884501334204	25.727874291609915
18	22.01474119406641	25.277881899728705	29.878381437674662	22.828995468530223
19	24.187508582329123	27.162097465568625	25.970406273543418	22.679987678558835
20	24.849414917108618	26.278813430370867	26.44823324636573	22.423538406154783
21	23.77555678440076	27.89600258304911	27.18993204650973	21.1385085860404
22	21.62561375250975	26.039436034277358	26.167660670479385	26.167289542733503
23	20.961109523509087	28.84813081510787	26.66367290284989	23.527086758533155
24	22.181377551967163	25.56012455047152	28.421705035090127	23.83679286247119
25	21.017149813137177	28.091958032874498	27.27807488615656	23.61281726783176
26	20.241863951990915	27.69299570605198	28.989530486288682	23.075609855668418
27	22.789470363593853	27.658109697939125	26.78243378153194	22.76998615693508
28	19.435960051809435	27.739201110414214	30.81158215469347	22.013256683082886
29	21.354876061889264	26.822701141960074	28.041855787180502	23.78056700897016
30	22.600380777067276	26.533778191791395	27.87577612089858	22.990064910242754
31	23.1129081941295	27.058738388340654	26.758124914176705	23.07022850335314
32	22.952024316289908	28.014206770112338	26.509654888309104	22.524114025288643
33	23.03645587847793	25.679998812391215	25.3608289509332	25.922716358197658
34	20.401634446592862	25.909726887091804	28.915861628731225	24.772777037584106
35	20.607795909429985	25.622659575652534	27.0407386926654	26.728805822252077
36	23.547684348429573	26.24800982746271	26.564952922445435	23.63935290166228
37	21.957401957327733	27.22426136300376	28.47885870795587	22.339477971712643
38	21.02958259262421	28.52228065422399	25.558825603360937	24.889311149790867
39	21.958329776692434	25.296809414768656	27.288280899168306	25.456579909370603
40	21.997669317755864	24.282702849147704	28.776503160152757	24.94312467294367
41	19.54785506719268	25.47439404117291	27.246900155502523	27.730850736131885
42	21.861465435017386	24.81341552575812	26.96948216545617	26.35563687376832
43	22.737698043043398	24.4285560532791	28.081380892116876	24.752365011560627
44	22.08358539092741	25.069679234289232	28.141874714695547	24.70486066008781
45	22.032369761995778	25.833089007567295	26.69020853668041	25.444332693756515
46	22.479022004164054	25.664782574810076	27.403887192010362	24.45230822901551
47	20.903399159024527	24.811745450901654	29.567747514371924	24.717107875701895
48	20.85923495726464	25.211450033215932	27.80117944397641	26.128135565543015
49	21.327041480948157	24.414638762808547	29.539912933430816	24.71840682281248
50	21.291969908962365	24.02384124639542	29.27010306217503	25.414085782467183
51	20.413324970588125	24.683335250826687	26.938864126420953	27.964475652164232
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2705.0
1	2809.0
2	2913.0
3	2744.0
4	2575.0
5	1908.5
6	1242.0
7	1241.5
8	1241.0
9	1383.5
10	1526.0
11	1527.0
12	1528.0
13	1598.0
14	1668.0
15	1625.5
16	1583.0
17	1569.5
18	1556.0
19	1555.5
20	1555.0
21	1663.5
22	1772.0
23	2037.5
24	2303.0
25	3047.5
26	4193.0
27	4594.0
28	5700.0
29	6806.0
30	7486.0
31	8166.0
32	8600.5
33	9035.0
34	10427.0
35	11819.0
36	13253.5
37	14688.0
38	15424.5
39	16161.0
40	18445.5
41	20730.0
42	24769.5
43	28809.0
44	33230.0
45	37651.0
46	42578.5
47	47506.0
48	50391.0
49	53276.0
50	52341.5
51	51407.0
52	44160.5
53	36914.0
54	33328.5
55	29743.0
56	27494.5
57	25246.0
58	24166.5
59	23087.0
60	21658.0
61	20229.0
62	18690.0
63	17151.0
64	15201.5
65	13252.0
66	11737.0
67	10222.0
68	8788.5
69	7355.0
70	6376.5
71	5398.0
72	5005.0
73	4612.0
74	3922.0
75	2387.5
76	1543.0
77	1369.0
78	1195.0
79	877.0
80	559.0
81	433.5
82	308.0
83	236.5
84	165.0
85	99.0
86	33.0
87	23.5
88	14.0
89	17.0
90	20.0
91	15.5
92	11.0
93	5.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	538898.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.254809513945887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.7948484538791	20.385193472349247
2	10.090514985670024	5.500301276641058
3	3.8296701267352073	3.131307894162512
4	1.9397402136495459	2.1146900011823626
5	1.0861686815639393	1.4801660258019458
6	0.7634043355989142	1.2483863849321293
7	0.5699812627526658	1.0874311519989572
8	0.4183618747675875	0.9121898563750307
9	0.407269480088884	0.9990046890599678
>10	4.6264508228548396	30.466035477818277
>50	1.3280753352721422	24.28438542938047
>100	0.13595009862096516	5.572278129370893
>500	0.005465330597827745	1.0261243950600791
>1k	0.004098997948370809	1.7925058158670626
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2208	0.4097250314530764	No Hit
CCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	2068	0.38374608924137776	TruSeq Adapter, Index 23 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	1778	0.32993256608857335	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGCT	1291	0.23956295996645005	TruSeq Adapter, Index 20 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1196	0.22193439203708307	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1086	0.20152236601360557	No Hit
TCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	807	0.14975004546314885	TruSeq Adapter, Index 23 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	784	0.14548207638551267	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCC	765	0.14195636279963927	No Hit
AAAACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC	726	0.13471937175495177	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	653	0.12117320903028031	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	619	0.11486403735029634	No Hit
ACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	604	0.11208057925618577	TruSeq Adapter, Index 20 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	553	0.10261682173620983	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.711277458814098E-4	0.0	0.0	0.2453154400276119	0.0
2	5.566916188221148E-4	0.0	0.0	1.2529272700956395	0.0
3	5.566916188221148E-4	0.0	0.0	1.5787774309795175	0.0
4	7.422554917628196E-4	0.0	0.0	2.014295840771352	0.0
5	7.422554917628196E-4	0.0	0.0	3.367056474509091	0.0
6	7.422554917628196E-4	0.0	0.0	4.443698065311061	0.0
7	7.422554917628196E-4	0.0	0.0	5.344053976819361	0.0
8	7.422554917628196E-4	0.0	0.0	6.6485680035925165	0.0
9	7.422554917628196E-4	0.0	0.0	7.16814684782649	0.0
10	7.422554917628196E-4	0.0	0.0	8.362435934072867	0.0
11	7.422554917628196E-4	0.0	0.0	9.69478454178713	0.0
12	7.422554917628196E-4	0.0	0.0	10.805013193591366	0.0
13	7.422554917628196E-4	0.0	0.0	11.220676268978545	0.0
14	7.422554917628196E-4	0.0	0.0	11.37432315577345	0.0
15	7.422554917628196E-4	0.0	0.0	11.658235881372727	0.0
16	7.422554917628196E-4	0.0	0.0	12.281544930580555	0.0
17	7.422554917628196E-4	0.0	0.0	13.0412434263998	0.0
18	7.422554917628196E-4	0.0	0.0	13.978526548623302	0.0
19	7.422554917628196E-4	0.0	0.0	14.39400406013754	0.0
20	7.422554917628196E-4	0.0	0.0	14.792780823087115	0.0
21	7.422554917628196E-4	0.0	0.0	15.300483579452884	0.0
22	7.422554917628196E-4	0.0	0.0	15.82321700952685	0.0
23	7.422554917628196E-4	0.0	0.0	16.38399103355366	0.0
24	7.422554917628196E-4	0.0	0.0	16.79520057599026	0.0
25	7.422554917628196E-4	0.0	0.0	17.198616435763356	0.0
26	7.422554917628196E-4	0.0	0.0	17.538940578736607	0.0
27	7.422554917628196E-4	0.0	0.0	17.88408938240632	0.0
28	7.422554917628196E-4	0.0	0.0	18.244083295911285	0.0
29	7.422554917628196E-4	0.0	0.0	18.59832472935509	0.0
30	7.422554917628196E-4	0.0	0.0	19.005822994332878	0.0
31	7.422554917628196E-4	0.0	0.0	19.365074652346085	0.0
32	7.422554917628196E-4	0.0	0.0	19.746965102858056	0.0
33	7.422554917628196E-4	0.0	0.0	20.091928342654825	0.0
34	7.422554917628196E-4	0.0	0.0	20.463427216282117	0.0
35	7.422554917628196E-4	0.0	0.0	20.845503230667028	0.0
36	7.422554917628196E-4	0.0	0.0	21.21922887076961	0.0
37	7.422554917628196E-4	0.0	0.0	21.566047749295784	0.0
38	9.278193647035246E-4	0.0	0.0	21.92010361886665	0.0
39	9.278193647035246E-4	0.0	0.0	22.257458739872853	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCA	30	2.1633477E-6	45.000004	6
TCGCACG	25	3.888037E-5	45.000004	1
TAGACTA	25	3.888037E-5	45.000004	35
TCGATAG	25	3.888037E-5	45.000004	43
ATGTACG	30	2.1633477E-6	45.000004	1
CACTCGC	25	3.888037E-5	45.000004	28
TTACGTT	25	3.888037E-5	45.000004	34
GCTACGA	100	0.0	45.000004	10
AATAGCG	30	2.1633477E-6	45.000004	45
CCTATCA	25	3.888037E-5	45.000004	21
GCTCGAT	25	3.888037E-5	45.000004	41
GTTGGCG	35	1.2103192E-7	45.000004	1
CAATACG	30	2.1633477E-6	45.000004	1
CGATAGC	30	2.1633477E-6	45.000004	10
ACGAACT	25	3.888037E-5	45.000004	1
AACACGA	35	1.2103192E-7	45.000004	45
ACAACGA	90	0.0	45.0	13
TTCGTTA	20	7.029949E-4	45.0	1
TGACGAT	20	7.029949E-4	45.0	37
ACGGGTA	105	0.0	45.0	5
>>END_MODULE