FastQCFastQC Report
Sat 18 Jun 2016
SRR3553638_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553638_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615002
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22660.36845408632817456No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13930.22650332844446033No Hit
CCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC11430.1858530541364093No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10950.17804820146926353No Hit
GCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC10760.17495878062185163No Hit
GGTCTTTGGGGATGAAGGCGGGTGGATCTCTAAGTTGGGTTCAAGACTTGC8490.1380483315501413No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCC8320.13528411289719383No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC7830.12731665913281584No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7270.11821099768781239No Hit
GTTAGGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAA6890.11203215599298864No Hit
CTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGCT6420.10438990442307504Illumina Single End Adapter 1 (95% over 21bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT6310.10260129235352079No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTCG253.888682E-545.0000049
CGCATGG302.1638498E-645.0000042
TTATGCA253.888682E-545.00000444
GCACGGT253.888682E-545.00000421
CCCGTCA502.1827873E-1145.00000412
ATGACGG502.1827873E-1145.0000042
GCGATAC302.1638498E-645.0000049
CAGTACG302.1638498E-645.0000041
CGATCAT253.888682E-545.00000410
TATAACG302.1638498E-645.0000041
CGATACA253.888682E-545.00000410
ACGGTCG253.888682E-545.00000423
GCGAATG453.8380676E-1045.01
CGTGCCG207.030726E-445.025
ATTACGA207.030726E-445.023
GACCGAA207.030726E-445.09
GTTAACG351.2107012E-745.01
CCCCCGT551.8189894E-1245.010
TTACGAA207.030726E-445.024
CCGGTCA207.030726E-445.035