##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553638_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	615002
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.891127183326233	33.0	31.0	34.0	30.0	34.0
2	31.96714319628229	33.0	31.0	34.0	30.0	34.0
3	31.965487917112466	33.0	31.0	34.0	30.0	34.0
4	35.653513321907894	37.0	35.0	37.0	33.0	37.0
5	35.55898517403195	37.0	35.0	37.0	33.0	37.0
6	35.54304864049222	37.0	35.0	37.0	33.0	37.0
7	35.83609646797897	37.0	35.0	37.0	35.0	37.0
8	35.76209996065054	37.0	35.0	37.0	35.0	37.0
9	37.463548086022485	39.0	37.0	39.0	35.0	39.0
10	36.96506515425966	39.0	37.0	39.0	33.0	39.0
11	36.85746875619917	39.0	37.0	39.0	33.0	39.0
12	36.76601214304994	39.0	35.0	39.0	33.0	39.0
13	36.705919655545834	39.0	35.0	39.0	33.0	39.0
14	37.75988208168429	40.0	36.0	41.0	33.0	41.0
15	37.87834023303989	40.0	36.0	41.0	33.0	41.0
16	37.9331042825877	40.0	36.0	41.0	33.0	41.0
17	37.875112601259836	40.0	36.0	41.0	33.0	41.0
18	37.8536069801399	40.0	36.0	41.0	33.0	41.0
19	37.77399097889113	40.0	36.0	41.0	33.0	41.0
20	37.711417198643254	40.0	36.0	41.0	33.0	41.0
21	37.527611292321	39.0	35.0	41.0	33.0	41.0
22	37.53654622261391	39.0	35.0	41.0	33.0	41.0
23	37.450816420109206	39.0	35.0	41.0	33.0	41.0
24	37.455473315533936	39.0	35.0	41.0	33.0	41.0
25	37.32575341218403	39.0	35.0	41.0	32.0	41.0
26	37.248522769031645	39.0	35.0	41.0	32.0	41.0
27	37.2365878484948	39.0	35.0	41.0	32.0	41.0
28	37.124786586059884	39.0	35.0	41.0	32.0	41.0
29	37.07222252935763	39.0	35.0	41.0	32.0	41.0
30	36.94791236451264	39.0	35.0	41.0	31.0	41.0
31	36.85720046438873	39.0	35.0	41.0	31.0	41.0
32	36.71409686472564	39.0	35.0	41.0	31.0	41.0
33	36.61424678293729	39.0	35.0	41.0	31.0	41.0
34	36.4901951538369	39.0	35.0	41.0	30.0	41.0
35	36.3922865291495	39.0	35.0	41.0	30.0	41.0
36	36.247457731844776	39.0	35.0	41.0	30.0	41.0
37	36.20004487790283	39.0	35.0	41.0	30.0	41.0
38	36.10070536355979	38.0	35.0	40.0	30.0	41.0
39	36.006087785080375	38.0	35.0	40.0	29.0	41.0
40	35.84688830280227	38.0	35.0	40.0	29.0	41.0
41	35.736246386190615	38.0	35.0	40.0	29.0	41.0
42	35.607786641344255	38.0	35.0	40.0	28.0	41.0
43	35.54280636485735	38.0	35.0	40.0	28.0	41.0
44	35.42420024650326	38.0	34.0	40.0	28.0	41.0
45	35.375990647184885	38.0	34.0	40.0	27.0	41.0
46	35.198532687698574	38.0	34.0	40.0	27.0	41.0
47	35.10401104386652	37.0	34.0	40.0	26.0	41.0
48	35.02673649841789	37.0	34.0	40.0	26.0	41.0
49	34.94913349875285	37.0	34.0	40.0	26.0	41.0
50	34.81569816033119	37.0	34.0	40.0	26.0	41.0
51	33.37127196334321	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	5.0
10	4.0
11	4.0
12	9.0
13	17.0
14	24.0
15	44.0
16	98.0
17	193.0
18	329.0
19	576.0
20	924.0
21	1487.0
22	2070.0
23	2951.0
24	3827.0
25	4871.0
26	5973.0
27	7315.0
28	8551.0
29	9832.0
30	12160.0
31	15101.0
32	19513.0
33	26466.0
34	44625.0
35	55064.0
36	52032.0
37	73040.0
38	115401.0
39	152440.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.32717617178481	18.6472564316864	23.596833831434694	14.428733565094099
2	29.912423049030735	27.28804133970296	25.785281999082933	17.014253612183374
3	30.96315133934524	26.0875574388376	26.47601146012533	16.47327976169183
4	27.801047801470563	27.588853369582537	24.90317104659822	19.706927782348675
5	23.78089827350155	30.75843655792989	25.671298629923157	19.789366538645403
6	24.276994221157004	36.042809616879296	24.937479878114218	14.742716283849482
7	79.82266724335855	7.3094071238792715	8.379159742569943	4.488765890192227
8	81.8647744234978	6.103882588999711	7.406317377829666	4.625025609672814
9	76.4986130126406	8.377858933792085	9.435904273482038	5.687623780085268
10	41.72734397611715	25.744306522580413	16.17929697789601	16.349052523406428
11	31.55306812010367	27.000074796504727	22.26122841876937	19.18562866462223
12	28.4677123001226	23.89390603607793	28.0455998517078	19.592781812091665
13	25.08186965245641	25.97845210259479	28.561045329933886	20.37863291501491
14	22.02887145082455	28.33925743330916	28.33128997954478	21.300581136321508
15	20.111479312262396	26.707718023681227	32.43062624186588	20.750176422190496
16	22.264643041811247	25.255365023203176	30.565266454418033	21.914725480567544
17	21.91537588495647	24.85195170097008	28.099583415988892	25.133088998084556
18	23.852442756283722	24.793577907063717	28.740231739083775	22.61374759756879
19	24.62268415387267	28.124298782768186	25.594063108737856	21.658953954621285
20	26.863164672635207	25.886907684853057	25.678127876006908	21.571799766504824
21	24.738618736199232	27.742348805369737	26.513409712488738	21.00562274594229
22	23.049518538151094	25.636664596212693	25.388697922933584	25.925118942702625
23	22.67504821122533	27.93649451546499	25.184958748101632	24.203498525208047
24	22.987567520105625	26.26397962933454	28.10429884780862	22.64415400275121
25	23.17439618082543	27.007229244782945	26.08999645529608	23.72837811909555
26	21.087085895655626	28.923808377858933	26.25682518105632	23.73228054542912
27	22.98935613217518	27.134871106110225	26.719913105973642	23.155859655740958
28	20.568388395484895	27.460886306060793	28.535842159862895	23.43488313859142
29	21.988058575419267	27.419585627363812	27.325927395358065	23.266428401858853
30	23.47407000302438	25.80674534391758	26.83942491243931	23.87975974061873
31	24.15862062237196	27.21357003717061	24.92349618375225	23.704313156705183
32	24.77016334906228	27.619747578056657	25.31715344015141	22.292935632729648
33	24.178132754039826	26.718612297195783	25.134227205765185	23.96902774299921
34	22.284317774576344	27.153245030097462	26.556661604352506	24.005775590973688
35	23.874068702215602	25.829021694238392	26.432922169358797	23.863987434187205
36	24.731626889018248	27.63600768777988	25.24821057492496	22.384154848276914
37	23.005453640801168	27.738934182327863	27.047879519090994	22.207732657779975
38	23.643825548534803	27.16609051677881	26.02333000543088	23.166753929255517
39	23.59894764569871	25.208048103908602	27.190318080266408	24.002686170126275
40	23.41065557510382	25.077479422831146	28.899580814371333	22.6122841876937
41	21.67391325556665	25.63552638853207	27.639584911918984	25.0509754439823
42	22.206919652293813	26.29747545536437	27.342837909470212	24.1527669828716
43	22.96577897307651	24.949187157114935	27.9299904715757	24.155043398232852
44	22.404317384333712	25.188048168949045	27.127391455637547	25.280242991079703
45	23.519923512443864	25.015203202591213	27.032595015951166	24.43227826901376
46	23.25065609542733	26.076663165323037	26.698774963333456	23.973905775916176
47	22.71960091186695	25.696989603285843	28.478281371442694	23.10512811340451
48	22.530170633591435	24.56739978081372	28.385761347117572	24.516668238477273
49	21.864156539328327	24.95845541965717	28.78624784960049	24.391140191414014
50	21.564645318226606	24.94219530993395	28.777467390349948	24.715691981489492
51	21.459605009414602	25.1600807802251	27.133732898429596	26.246581311930694
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2596.0
1	2730.0
2	2864.0
3	3092.5
4	3321.0
5	2431.5
6	1542.0
7	1564.5
8	1587.0
9	1737.5
10	1888.0
11	1937.0
12	1986.0
13	1961.5
14	1937.0
15	1971.0
16	2005.0
17	2066.5
18	2128.0
19	2141.5
20	2155.0
21	2313.5
22	2472.0
23	2789.5
24	3107.0
25	3386.5
26	4389.5
27	5113.0
28	6087.5
29	7062.0
30	8744.0
31	10426.0
32	11029.5
33	11633.0
34	13155.0
35	14677.0
36	16079.5
37	17482.0
38	18044.0
39	18606.0
40	20800.0
41	22994.0
42	26459.5
43	29925.0
44	34295.0
45	38665.0
46	41578.0
47	44491.0
48	48378.5
49	52266.0
50	51390.0
51	50514.0
52	47569.0
53	44624.0
54	40874.0
55	37124.0
56	34760.5
57	32397.0
58	29972.0
59	27547.0
60	27141.5
61	26736.0
62	24372.0
63	22008.0
64	19777.0
65	17546.0
66	15254.5
67	12963.0
68	11072.5
69	9182.0
70	8909.0
71	8636.0
72	7826.0
73	7016.0
74	5694.0
75	3553.5
76	2735.0
77	2415.0
78	2095.0
79	1788.0
80	1481.0
81	1137.0
82	793.0
83	572.0
84	351.0
85	262.0
86	173.0
87	116.5
88	60.0
89	41.0
90	22.0
91	17.0
92	12.0
93	10.5
94	9.0
95	7.5
96	6.0
97	5.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	615002.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.80064237693438
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.53051684538752	19.487358536728614
2	9.330704015861798	4.814763148765514
3	3.2600546311392233	2.523345110018755
4	1.655900079091486	1.7089314301030711
5	0.9983736112754767	1.2879340251543538
6	0.7381406884173644	1.142670235543234
7	0.6126329676443459	1.1064426874558246
8	0.4548088479457223	0.9387488348570486
9	0.39929426713097205	0.9271843730465684
>10	5.488243009208406	34.94943643605474
>50	1.3991889284288028	23.961703829622895
>100	0.12329048397813672	4.983362313916195
>500	0.0056903300297601565	1.0306354741340966
>1k	0.003161294460977865	1.1374835645990908
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2266	0.36845408632817456	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1393	0.22650332844446033	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	1143	0.1858530541364093	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1095	0.17804820146926353	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	1076	0.17495878062185163	No Hit
GGTCTTTGGGGATGAAGGCGGGTGGATCTCTAAGTTGGGTTCAAGACTTGC	849	0.1380483315501413	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCC	832	0.13528411289719383	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	783	0.12731665913281584	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	727	0.11821099768781239	No Hit
GTTAGGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAA	689	0.11203215599298864	No Hit
CTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGCT	642	0.10438990442307504	Illumina Single End Adapter 1 (95% over 21bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	631	0.10260129235352079	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10829233075664794	0.0
2	0.0	0.0	0.0	0.6230874045938062	0.0
3	0.0	0.0	0.0	0.8291029947870088	0.0
4	0.0	0.0	0.0	1.1050370567900591	0.0
5	0.0	0.0	0.0	1.9756033313712802	0.0
6	0.0	0.0	0.0	2.624544310425007	0.0
7	0.0	0.0	0.0	3.190233527695845	0.0
8	0.0	0.0	0.0	4.023076347719194	0.0
9	0.0	0.0	0.0	4.374294717740756	0.0
10	0.0	0.0	0.0	5.230226893571078	0.0
11	0.0	0.0	0.0	6.31851603734622	0.0
12	0.0	0.0	0.0	7.108757369894732	0.0
13	0.0	0.0	0.0	7.419325465608242	0.0
14	0.0	0.0	0.0	7.546154321449361	0.0
15	0.0	0.0	0.0	7.754283725906582	0.0
16	0.0	0.0	0.0	8.209729399253986	0.0
17	0.0	0.0	0.0	8.747613828898118	0.0
18	0.0	0.0	0.0	9.391676775034878	0.0
19	0.0	0.0	0.0	9.746472369195548	0.0
20	0.0	0.0	0.0	10.067446935131919	0.0
21	0.0	0.0	0.0	10.466307426642516	0.0
22	0.0	0.0	0.0	10.887606869571156	0.0
23	1.6260109723220412E-4	0.0	0.0	11.30256486970774	0.0
24	3.2520219446440825E-4	0.0	0.0	11.655409250701624	0.0
25	3.2520219446440825E-4	0.0	0.0	11.928904296246191	0.0
26	3.2520219446440825E-4	0.0	0.0	12.204187953860313	0.0
27	3.2520219446440825E-4	0.0	0.0	12.4893902784056	0.0
28	3.2520219446440825E-4	0.0	0.0	12.770527575520079	0.0
29	4.8780329169661237E-4	0.0	0.0	13.092802950234308	0.0
30	4.8780329169661237E-4	0.0	0.0	13.431988839060686	0.0
31	4.8780329169661237E-4	0.0	0.0	13.732150464551335	0.0
32	6.504043889288165E-4	0.0	0.0	14.022393423110818	0.0
33	8.130054861610206E-4	0.0	0.0	14.32857128919906	0.0
34	8.130054861610206E-4	0.0	0.0	14.625643493842297	0.0
35	8.130054861610206E-4	0.0	0.0	14.94157742576447	0.0
36	8.130054861610206E-4	0.0	0.0	15.26255199170084	0.0
37	8.130054861610206E-4	0.0	0.0	15.568892458886312	0.0
38	8.130054861610206E-4	0.0	0.0	15.88141176776661	0.0
39	8.130054861610206E-4	0.0	0.0	16.214744017092627	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTCG	25	3.888682E-5	45.000004	9
CGCATGG	30	2.1638498E-6	45.000004	2
TTATGCA	25	3.888682E-5	45.000004	44
GCACGGT	25	3.888682E-5	45.000004	21
CCCGTCA	50	2.1827873E-11	45.000004	12
ATGACGG	50	2.1827873E-11	45.000004	2
GCGATAC	30	2.1638498E-6	45.000004	9
CAGTACG	30	2.1638498E-6	45.000004	1
CGATCAT	25	3.888682E-5	45.000004	10
TATAACG	30	2.1638498E-6	45.000004	1
CGATACA	25	3.888682E-5	45.000004	10
ACGGTCG	25	3.888682E-5	45.000004	23
GCGAATG	45	3.8380676E-10	45.0	1
CGTGCCG	20	7.030726E-4	45.0	25
ATTACGA	20	7.030726E-4	45.0	23
GACCGAA	20	7.030726E-4	45.0	9
GTTAACG	35	1.2107012E-7	45.0	1
CCCCCGT	55	1.8189894E-12	45.0	10
TTACGAA	20	7.030726E-4	45.0	24
CCGGTCA	20	7.030726E-4	45.0	35
>>END_MODULE