##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553636_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	893820
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76952518404153	31.0	31.0	34.0	30.0	34.0
2	31.856125394374708	33.0	31.0	34.0	30.0	34.0
3	31.86365823096373	33.0	31.0	34.0	30.0	34.0
4	35.55149470810678	37.0	35.0	37.0	33.0	37.0
5	35.457847217560584	37.0	35.0	37.0	33.0	37.0
6	35.43761607482491	37.0	35.0	37.0	33.0	37.0
7	35.769078785437785	37.0	35.0	37.0	35.0	37.0
8	35.68560672171131	37.0	35.0	37.0	35.0	37.0
9	37.361653353024096	39.0	37.0	39.0	34.0	39.0
10	36.823473406278666	39.0	37.0	39.0	32.0	39.0
11	36.784278713835	39.0	37.0	39.0	32.0	39.0
12	36.74690206081761	39.0	35.0	39.0	33.0	39.0
13	36.75374572061489	39.0	35.0	39.0	33.0	39.0
14	37.80285404220089	40.0	37.0	41.0	33.0	41.0
15	37.95209885659305	40.0	37.0	41.0	33.0	41.0
16	37.971966391443466	40.0	37.0	41.0	33.0	41.0
17	37.90744557069656	40.0	37.0	41.0	33.0	41.0
18	37.85882056789958	39.0	36.0	41.0	33.0	41.0
19	37.78156899599472	39.0	36.0	41.0	33.0	41.0
20	37.71821395806762	39.0	36.0	41.0	33.0	41.0
21	37.54988252668323	39.0	36.0	41.0	33.0	41.0
22	37.56194983329977	39.0	35.0	41.0	33.0	41.0
23	37.48869571054575	39.0	35.0	41.0	33.0	41.0
24	37.438188897093376	39.0	35.0	41.0	32.0	41.0
25	37.30073169094449	39.0	35.0	41.0	32.0	41.0
26	37.25319191783581	39.0	35.0	41.0	32.0	41.0
27	37.242552191716456	39.0	35.0	41.0	32.0	41.0
28	37.14570047660603	39.0	35.0	41.0	32.0	41.0
29	37.07772258396545	39.0	35.0	41.0	32.0	41.0
30	36.90531538788571	39.0	35.0	41.0	31.0	41.0
31	36.823605423910855	39.0	35.0	41.0	31.0	41.0
32	36.67901814683046	39.0	35.0	41.0	31.0	41.0
33	36.59793246962476	39.0	35.0	41.0	30.0	41.0
34	36.49533798751426	39.0	35.0	41.0	30.0	41.0
35	36.386279116600655	39.0	35.0	41.0	30.0	41.0
36	36.25239309928173	39.0	35.0	40.0	30.0	41.0
37	36.166474234185856	39.0	35.0	40.0	30.0	41.0
38	36.018360520015214	38.0	35.0	40.0	29.0	41.0
39	35.94314627106129	38.0	35.0	40.0	29.0	41.0
40	35.79735181580184	38.0	35.0	40.0	29.0	41.0
41	35.70007943433801	38.0	35.0	40.0	28.0	41.0
42	35.58518717415139	38.0	35.0	40.0	28.0	41.0
43	35.46891767917478	38.0	34.0	40.0	27.0	41.0
44	35.35508715401311	38.0	34.0	40.0	27.0	41.0
45	35.27989080575507	38.0	34.0	40.0	27.0	41.0
46	35.11542816226981	38.0	34.0	40.0	26.0	41.0
47	35.01778769774675	37.0	34.0	40.0	26.0	41.0
48	34.928842496252045	37.0	34.0	40.0	26.0	41.0
49	34.856263005974355	37.0	34.0	40.0	26.0	41.0
50	34.734258575552126	37.0	34.0	40.0	26.0	41.0
51	33.14056968964668	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	9.0
11	10.0
12	9.0
13	17.0
14	20.0
15	55.0
16	116.0
17	238.0
18	427.0
19	829.0
20	1276.0
21	1981.0
22	3044.0
23	4152.0
24	5785.0
25	7315.0
26	8771.0
27	10450.0
28	12649.0
29	15192.0
30	18299.0
31	22876.0
32	28719.0
33	39015.0
34	62583.0
35	77234.0
36	80518.0
37	112052.0
38	174018.0
39	206099.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.68006981271397	19.78015707860643	23.45226108164955	14.08751202703005
2	29.9319773556197	26.36806068335907	25.566333266205728	18.133628694815513
3	31.28627687901367	25.45624398648497	26.386520775995166	16.870958358506186
4	28.09469468125573	26.183012239600817	26.485534000134255	19.236759079009197
5	22.97173927636437	31.535320310577074	25.13235327023338	20.360587142825175
6	23.413438947439083	36.159517576245776	25.344700275223197	15.082343201091941
7	79.56691503882215	7.24418786780336	8.390168042782662	4.798729050591842
8	80.4070170727887	6.368060683359065	8.057326978586293	5.167595265265938
9	74.43937258060907	8.592222147635988	10.421673267548275	6.546732004206664
10	38.40157973641226	25.816159853214295	18.336130317066075	17.446130093307378
11	28.824595556152243	26.644402676154034	23.500145443154103	21.030856324539617
12	25.422680181692066	22.326307310196682	31.005012194849073	21.24600031326218
13	22.837372177843413	25.180125752388626	31.141393121657607	20.841108948110357
14	20.452328209259136	28.381105815488578	28.832427110603927	22.334138864648363
15	19.051822514600257	26.011836835156966	32.934035935646996	22.00230471459578
16	20.05280705287418	25.24233067060482	31.162090801279902	23.5427714752411
17	20.213242039784298	24.917768678257367	28.587970732362223	26.281018549596112
18	21.597637108142578	24.90188181065539	29.79089749614016	23.709583585061868
19	23.286903403369806	27.012038217985722	26.66565975252288	23.03539862612159
20	24.245933185652593	26.993913763397554	26.148105882616186	22.612047168333667
21	23.04546776755946	28.108903358618065	27.65713454610548	21.188494327716988
22	21.534537155131904	25.542950482199995	25.08838468595467	27.834127676713432
23	20.879931082320823	27.753686424559753	26.533418361638812	24.83296413148061
24	22.007115526616097	25.914613680606834	28.551050547090018	23.52722024568705
25	22.359982994338907	26.97433487726835	26.311897250005593	24.353784878387145
26	19.844263945760893	27.291512832561367	27.52097737799557	25.343245843682173
27	21.688259381083437	26.428251773287688	27.22136448054418	24.662124365084694
28	20.53086751247455	26.565080217493453	29.16739388243718	23.736658387594815
29	21.352397574455708	25.573157906513615	28.126692175158308	24.947752343872367
30	22.07021547962677	25.161330021704593	28.33053634960059	24.437918149068043
31	23.854243583719317	26.491799243695596	24.61312121008704	25.040835962498043
32	23.28466581638361	25.43051173614374	27.49345505806538	23.791367389407263
33	22.241167125371998	24.006511378129826	26.17238370141639	27.579937795081783
34	21.09429191559822	23.84954465104831	28.557427670000674	26.498735763352798
35	21.106710523371596	22.20670828578461	29.34483453044237	27.341746660401423
36	22.85762234006847	24.231165111543714	27.64102391980488	25.270188628582936
37	21.954084715043297	24.90154617260746	28.288134076212213	24.85623503613703
38	21.859882302924525	26.28347989528093	28.173681501868387	23.682956299926158
39	22.753798303909065	23.08417802242062	27.776733570517553	26.38529010315276
40	22.51694972142042	22.650645543845517	31.10380166028954	23.728603074444518
41	19.91407665973015	23.320467208162718	28.763509431429146	28.001946700677987
42	21.515070148352017	24.078449799735964	28.08417802242062	26.322302029491397
43	21.71913808149292	22.51269830614665	29.078561679085276	26.68960193327516
44	22.519522946454543	22.64449217963348	28.6718802443445	26.164104629567476
45	22.36166118457855	22.932469624756664	28.415452775726656	26.29041641493813
46	23.26642948244613	23.38804233514578	28.48560112774384	24.85992705466425
47	21.4007294533575	23.18330312590902	29.966771833255017	25.449195587478464
48	20.994048018616724	23.26642948244613	29.36799355574948	26.371528943187666
49	21.60860128437493	22.856391667226063	29.891365151820278	25.64364189657873
50	21.530509498556757	21.882817569533017	30.41216352285695	26.174509409053275
51	20.79065136157168	22.25492828533709	27.946566422769685	29.00785393032154
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3713.0
1	3693.0
2	3673.0
3	4156.0
4	4639.0
5	3528.0
6	2417.0
7	2537.5
8	2658.0
9	2785.5
10	2913.0
11	3014.0
12	3115.0
13	3241.0
14	3367.0
15	3213.0
16	3059.0
17	2981.5
18	2904.0
19	2946.0
20	2988.0
21	3168.0
22	3348.0
23	3553.5
24	3759.0
25	4460.0
26	5581.0
27	6001.0
28	8614.0
29	11227.0
30	11560.5
31	11894.0
32	14197.0
33	16500.0
34	18012.5
35	19525.0
36	21443.5
37	23362.0
38	24449.5
39	25537.0
40	28422.0
41	31307.0
42	36607.0
43	41907.0
44	48103.5
45	54300.0
46	63523.5
47	72747.0
48	80439.5
49	88132.0
50	86469.0
51	84806.0
52	74609.0
53	64412.0
54	57406.0
55	50400.0
56	45758.5
57	41117.0
58	39908.0
59	38699.0
60	35770.5
61	32842.0
62	31429.5
63	30017.0
64	27169.0
65	24321.0
66	20861.0
67	17401.0
68	16115.5
69	14830.0
70	14529.0
71	14228.0
72	12519.0
73	10810.0
74	9464.0
75	6228.5
76	4339.0
77	3739.0
78	3139.0
79	2466.5
80	1794.0
81	1384.5
82	975.0
83	817.0
84	659.0
85	488.5
86	318.0
87	260.5
88	203.0
89	165.0
90	127.0
91	92.0
92	57.0
93	49.5
94	42.0
95	24.0
96	6.0
97	5.5
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	893820.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.59284141910005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.10772898913828	20.17249972507836
2	10.918285260654098	6.025330275314521
3	4.106066872245892	3.3989415618630314
4	2.144990269602248	2.3674550541859
5	1.2559993540431817	1.7328295499302808
6	0.8805491222163748	1.457811137462651
7	0.6289158969827296	1.214750362797687
8	0.4732190578266492	1.0445966735285335
9	0.3942014153859178	0.9789423427735575
>10	4.788022921026781	31.723189160244917
>50	1.1715333156019687	21.449544843603814
>100	0.12029318736374679	5.164273126082573
>500	0.0061166027473091595	1.1710696478336184
>1k	0.004077735164872772	2.0987665393005845
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3277	0.36662862768790133	No Hit
CCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	3058	0.3421270501890761	Illumina PCR Primer Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	2128	0.23807925533105098	Illumina PCR Primer Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGCT	2110	0.23606542704347633	Illumina PCR Primer Index 10 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2033	0.22745071714662907	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1473	0.1647982815331946	No Hit
TCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	1267	0.14175113557539548	Illumina PCR Primer Index 10 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCC	1171	0.13101071804166387	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1101	0.12317916358998457	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1035	0.11579512653554407	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2375869861940883E-4	0.0	0.0	0.24512765433756237	0.0
2	2.2375869861940883E-4	0.0	0.0	1.0949631916940772	0.0
3	2.2375869861940883E-4	0.0	0.0	1.456892886710971	0.0
4	2.2375869861940883E-4	0.0	0.0	1.9435680562081852	0.0
5	2.2375869861940883E-4	0.0	0.0	3.43301783356828	0.0
6	2.2375869861940883E-4	0.0	0.0	4.55740529413081	0.0
7	3.3563804792911323E-4	0.0	0.0	5.557494797610257	0.0
8	3.3563804792911323E-4	0.0	0.0	6.960014320556712	0.0
9	3.3563804792911323E-4	0.0	0.0	7.570763688438388	0.0
10	3.3563804792911323E-4	0.0	0.0	9.003714394397083	0.0
11	4.4751739723881766E-4	0.0	0.0	10.642746861784252	0.0
12	4.4751739723881766E-4	0.0	0.0	11.959231165111543	0.0
13	4.4751739723881766E-4	0.0	0.0	12.449710232485288	0.0
14	4.4751739723881766E-4	0.0	0.0	12.637555659976282	0.0
15	4.4751739723881766E-4	0.0	0.0	12.941196214002819	0.0
16	4.4751739723881766E-4	0.0	0.0	13.659685395269742	0.0
17	4.4751739723881766E-4	0.0	0.0	14.519813832762749	0.0
18	4.4751739723881766E-4	0.0	0.0	15.706741849589402	0.0
19	4.4751739723881766E-4	0.0	0.0	16.208632610592737	0.0
20	5.593967465485221E-4	0.0	0.0	16.687923743035512	0.0
21	5.593967465485221E-4	0.0	0.0	17.252355060302968	0.0
22	5.593967465485221E-4	0.0	0.0	17.88458526325211	0.0
23	5.593967465485221E-4	0.0	0.0	18.52352822715983	0.0
24	5.593967465485221E-4	0.0	0.0	19.028663489293145	0.0
25	5.593967465485221E-4	0.0	0.0	19.448882325300396	0.0
26	6.712760958582265E-4	0.0	0.0	19.831733458638205	0.0
27	6.712760958582265E-4	0.0	0.0	20.247477120673068	0.0
28	6.712760958582265E-4	0.0	0.0	20.667695956680316	0.0
29	6.712760958582265E-4	0.0	0.0	21.087579154639638	0.0
30	6.712760958582265E-4	0.0	0.0	21.562171354411404	0.0
31	6.712760958582265E-4	0.0	0.0	22.014499563670537	0.0
32	7.83155445167931E-4	0.0	0.0	22.425768051733012	0.0
33	7.83155445167931E-4	0.0	0.0	22.841399834418564	0.0
34	7.83155445167931E-4	0.0	0.0	23.241816025597995	0.0
35	7.83155445167931E-4	0.0	0.0	23.703318341500527	0.0
36	7.83155445167931E-4	0.0	0.0	24.111454207782327	0.0
37	8.950347944776353E-4	0.0	0.0	24.509744691324876	0.0
38	8.950347944776353E-4	0.0	0.0	24.891924548566827	0.0
39	8.950347944776353E-4	0.0	0.0	25.27567071669911	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAA	35	1.2115197E-7	45.000004	33
TGTCACG	25	3.890108E-5	45.0	1
ACACGTA	20	7.0324435E-4	45.0	43
ACGCATG	25	3.890108E-5	45.0	1
CGTAATA	20	7.0324435E-4	45.0	28
ACGTAAG	45	3.8380676E-10	45.0	1
CCGTTAG	25	3.890108E-5	45.0	1
TACACGA	40	6.8121153E-9	45.0	9
TACGTAG	25	3.890108E-5	45.0	1
ATAACGC	110	0.0	42.954544	11
TCGTTTA	100	0.0	42.75	38
AATGCGG	125	0.0	41.399998	2
GCATCGC	115	0.0	41.08696	22
CACAACG	55	6.002665E-11	40.909092	12
TACGCAT	105	0.0	40.714287	16
CGTAAGG	145	0.0	40.344826	2
TCGTTGC	45	1.9274012E-8	40.0	16
TTACACG	295	0.0	38.898304	34
TCGGCAT	110	0.0	38.863636	32
CATCGTT	110	0.0	38.863636	36
>>END_MODULE