##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553633_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	842777
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91632306054864	33.0	31.0	34.0	30.0	34.0
2	32.00196730570483	33.0	31.0	34.0	30.0	34.0
3	31.985627277441125	33.0	31.0	34.0	30.0	34.0
4	35.66205769735055	37.0	35.0	37.0	33.0	37.0
5	35.572808702658	37.0	35.0	37.0	33.0	37.0
6	35.551831623311976	37.0	35.0	37.0	33.0	37.0
7	35.852832955811564	37.0	35.0	37.0	35.0	37.0
8	35.75484262147638	37.0	35.0	37.0	35.0	37.0
9	37.475993056288914	39.0	37.0	39.0	35.0	39.0
10	36.88720978384555	39.0	37.0	39.0	32.0	39.0
11	36.85963072081939	39.0	37.0	39.0	33.0	39.0
12	36.852685823177424	39.0	37.0	39.0	33.0	39.0
13	36.84418772700252	39.0	37.0	39.0	33.0	39.0
14	37.95355236319928	40.0	37.0	41.0	33.0	41.0
15	38.05399530362124	40.0	37.0	41.0	33.0	41.0
16	38.108917305526845	40.0	37.0	41.0	33.0	41.0
17	37.98056425365191	40.0	37.0	41.0	33.0	41.0
18	37.95703252461802	40.0	37.0	41.0	33.0	41.0
19	37.86398893182894	40.0	37.0	41.0	33.0	41.0
20	37.730267911915014	40.0	36.0	41.0	33.0	41.0
21	37.67119297275555	40.0	36.0	41.0	33.0	41.0
22	37.64923342711061	39.0	36.0	41.0	33.0	41.0
23	37.518782548645724	39.0	35.0	41.0	33.0	41.0
24	37.541914409149754	39.0	35.0	41.0	33.0	41.0
25	37.43672406816987	39.0	35.0	41.0	32.0	41.0
26	37.38233838844677	39.0	35.0	41.0	32.0	41.0
27	37.30455506023539	39.0	35.0	41.0	32.0	41.0
28	37.206402168070554	39.0	35.0	41.0	32.0	41.0
29	37.15621570118786	39.0	35.0	41.0	32.0	41.0
30	37.00287145947267	39.0	35.0	41.0	31.0	41.0
31	36.91927520565939	39.0	35.0	41.0	31.0	41.0
32	36.72213290111144	39.0	35.0	41.0	31.0	41.0
33	36.57679433586821	39.0	35.0	41.0	30.0	41.0
34	36.395456923954974	39.0	35.0	41.0	30.0	41.0
35	36.281261828455214	39.0	35.0	41.0	30.0	41.0
36	36.18904882311691	39.0	35.0	41.0	30.0	41.0
37	36.16584339629582	39.0	35.0	41.0	30.0	41.0
38	36.026191981983374	39.0	35.0	41.0	29.0	41.0
39	35.93015708781801	39.0	35.0	41.0	29.0	41.0
40	35.803042797798234	39.0	35.0	40.0	28.0	41.0
41	35.718855640341395	38.0	35.0	40.0	27.0	41.0
42	35.57491364856896	38.0	35.0	40.0	27.0	41.0
43	35.510312929754846	38.0	35.0	40.0	27.0	41.0
44	35.47380861129338	38.0	35.0	40.0	27.0	41.0
45	35.42433526306485	38.0	34.0	40.0	26.0	41.0
46	35.260993121549355	38.0	34.0	40.0	26.0	41.0
47	35.152233627638154	38.0	34.0	40.0	26.0	41.0
48	35.10874644182269	38.0	34.0	40.0	26.0	41.0
49	35.01672684470506	38.0	34.0	40.0	26.0	41.0
50	34.85368490122536	37.0	34.0	40.0	25.0	41.0
51	33.43058484035516	36.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	5.0
11	5.0
12	8.0
13	8.0
14	19.0
15	49.0
16	92.0
17	187.0
18	383.0
19	737.0
20	1368.0
21	2388.0
22	3767.0
23	5035.0
24	6261.0
25	7752.0
26	9050.0
27	10170.0
28	11321.0
29	12982.0
30	15971.0
31	19812.0
32	25779.0
33	34068.0
34	55052.0
35	67699.0
36	69418.0
37	99284.0
38	161059.0
39	222971.0
40	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.374846489640795	18.830366751821657	22.852664465214403	13.942122293323145
2	31.15770838549225	27.097203649363948	24.786390705963736	16.958697259180067
3	31.110246245448085	26.828330626013763	26.24632613372221	15.815096994815947
4	26.498943374107263	26.805311488092343	28.199749162589864	18.495995975210526
5	25.46403141044428	30.429876467915	25.557650481681392	18.548441639959325
6	23.03432580623344	38.034379201140986	25.085758154292296	13.845536838333272
7	80.2126778495379	6.135074877458687	10.01083323346508	3.6414140395383354
8	81.91906043947569	7.434232305817553	7.448470947830803	3.198236306875959
9	76.32018908916594	8.01647410880933	9.049250276170326	6.614086525854408
10	38.5189676509919	27.666749329893907	18.243972011576016	15.570311007538173
11	29.355926894065693	27.691073676666544	24.13141317335428	18.821586255913488
12	24.45415572565459	24.629172367067444	30.174292843777177	20.742379063500785
13	24.634393202472303	25.980300838774674	30.921465583422425	18.463840375330605
14	21.918846859845488	29.21342181858309	29.292802247806954	19.574929073764473
15	21.076393874061587	26.78181772877048	32.32432778777778	19.817460609390146
16	23.231530998116938	25.873866989725634	29.642479564582324	21.2521224475751
17	23.102315321846703	26.525878138582332	29.051338610332273	21.320467929238696
18	23.398004454321843	25.93983936438702	29.494753653694865	21.16740252759627
19	23.68123477503539	28.173644985565577	26.951257568728142	21.19386267067089
20	24.89163800151167	27.641119774270063	28.095688420543034	19.37155380367523
21	24.832191671106354	28.159050377502	27.945826713353593	19.062931238038058
22	23.00466196870584	25.601908927272575	28.588108123501232	22.805320980520353
23	22.55839919694059	27.848410670912948	28.266789435402245	21.326400696744216
24	22.13551152914709	27.554738679389683	29.002808572137113	21.30694121932611
25	22.19792424330517	29.342756150203435	26.959563443235872	21.499756163255523
26	21.39320365885638	27.982135250487378	28.915478234455854	21.709182856200396
27	22.664477079939296	27.609201485090363	28.137336448431792	21.588984986538552
28	20.524409185347963	29.461767466364176	29.303599884666998	20.710223463620864
29	22.579401193910133	27.1912973420015	28.814265220811674	21.415036243276692
30	21.92893256460487	28.135912584230464	28.720408838874338	21.214746012290323
31	23.214563283051152	28.503625514222623	25.937584912734923	22.344226289991305
32	24.251967009066455	28.728121436631515	26.384796927301053	20.635114627000974
33	23.761920413110467	27.11013708252598	26.23837622526481	22.88956627909874
34	21.70835226874962	28.525220787942718	26.860960847294123	22.905466096013534
35	22.32571605537408	27.190704065250948	27.230928228938378	23.252651650436594
36	22.658781623133997	30.162664619466362	25.53095302790655	21.647600729493092
37	23.0885513012339	28.77854996042844	26.333419160703247	21.799479577634415
38	22.37211029726725	29.150653138374683	26.546642824851652	21.930593739506417
39	23.46646859133555	27.235199821542356	26.706827547500705	22.591504039621395
40	22.04913043426672	27.446880966139325	28.01440950571741	22.489579093876554
41	21.155774303285448	27.511429476599385	27.051758650271662	24.281037569843505
42	21.336961022904042	28.478945201399657	27.491376722430726	22.692717053265575
43	22.928485233934957	26.630413502029597	27.439168368382145	23.001932895653297
44	21.979005122351463	27.12698614224166	27.373551959771092	23.520456775635783
45	22.35597316965223	26.830110456265416	27.4375071934806	23.376409180601748
46	23.2550247574388	27.95128485945867	26.742068186483493	22.051622196619032
47	21.300059209019707	28.221700402360295	28.922360244762256	21.55588014385775
48	22.023500878642867	27.6574942125853	27.732484393855078	22.586520514916756
49	22.856342781067827	26.639312653287877	28.468503530589945	22.03584103505435
50	22.031332131750155	26.307908260429507	28.29431747662786	23.366442131192475
51	21.51589329087054	26.932154057360368	26.89454031137537	24.657412340393723
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2755.0
1	3075.5
2	3396.0
3	4559.5
4	5723.0
5	4264.5
6	2806.0
7	2744.5
8	2683.0
9	2812.0
10	2941.0
11	3062.5
12	3184.0
13	3168.5
14	3153.0
15	3162.0
16	3171.0
17	3181.5
18	3192.0
19	3129.0
20	3066.0
21	3309.0
22	3552.0
23	4545.0
24	5538.0
25	6398.0
26	8471.5
27	9685.0
28	10915.0
29	12145.0
30	14100.0
31	16055.0
32	18913.5
33	21772.0
34	23164.0
35	24556.0
36	26140.5
37	27725.0
38	29624.0
39	31523.0
40	34881.5
41	38240.0
42	42495.0
43	46750.0
44	50532.0
45	54314.0
46	63589.0
47	72864.0
48	71638.0
49	70412.0
50	68802.0
51	67192.0
52	62762.5
53	58333.0
54	51816.5
55	45300.0
56	41188.5
57	37077.0
58	35197.0
59	33317.0
60	31794.0
61	30271.0
62	27151.5
63	24032.0
64	20788.5
65	17545.0
66	15550.0
67	13555.0
68	12157.5
69	10760.0
70	9546.5
71	8333.0
72	7440.0
73	6547.0
74	5564.5
75	3813.0
76	3044.0
77	2581.0
78	2118.0
79	1579.0
80	1040.0
81	842.0
82	644.0
83	493.5
84	343.0
85	214.5
86	86.0
87	81.5
88	77.0
89	88.5
90	100.0
91	57.5
92	15.0
93	7.5
94	0.0
95	1.0
96	2.0
97	3.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	842777.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.798687761637396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.4009140361212	20.960464662321954
2	8.657352644929022	4.81326086037535
3	3.2257375730671827	2.6901381478402984
4	1.6673756604062764	1.8540342145995208
5	1.1551141086781584	1.6055328218103107
6	0.8569710742319303	1.4293602787997046
7	0.732504937755935	1.425387323397441
8	0.5813291113743793	1.2928149163077198
9	0.501579439974807	1.2548925215564946
>10	6.201103822851354	39.28242892784118
>50	0.9194766823942	16.591226820833306
>100	0.09194766819718003	3.813731910264754
>500	0.005155944011056824	0.9274546909867152
>1k	0.0034372960073712163	2.0592719030652873
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCG	3686	0.43736362050696687	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2769	0.32855666445572196	No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTC	2525	0.29960475902878225	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2265	0.26875436800007596	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGC	2205	0.2616350469934514	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCC	1401	0.16623614550468271	No Hit
GCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC	1284	0.1523534695417649	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1106	0.13123281722211214	No Hit
GTCTTCGGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG	937	0.11118006305345304	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07546480267022	0.0
2	0.0	0.0	0.0	0.40497070992682527	0.0
3	0.0	0.0	0.0	0.5384579788010352	0.0
4	0.0	0.0	0.0	0.7862103498315687	0.0
5	0.0	0.0	0.0	1.5770482583174434	0.0
6	0.0	0.0	0.0	2.0008851689118234	0.0
7	0.0	0.0	0.0	2.431960055862939	0.0
8	0.0	0.0	0.0	3.03603444327503	0.0
9	0.0	0.0	0.0	3.2761928718984974	0.0
10	0.0	0.0	0.0	4.261269588515112	0.0
11	0.0	0.0	0.0	5.051988841650876	0.0
12	0.0	0.0	0.0	5.9197154170082955	0.0
13	0.0	0.0	0.0	6.173163244844129	0.0
14	0.0	0.0	0.0	6.292411871705089	0.0
15	1.186553501104088E-4	0.0	0.0	6.5261629114225945	0.0
16	1.186553501104088E-4	0.0	0.0	6.923776989642574	0.0
17	1.186553501104088E-4	0.0	0.0	7.419875008454194	0.0
18	1.186553501104088E-4	0.0	0.0	7.954891982102027	0.0
19	1.186553501104088E-4	0.0	0.0	8.355709754774988	0.0
20	2.373107002208176E-4	0.0	0.0	8.672282228869559	0.0
21	2.373107002208176E-4	0.0	0.0	9.038927260710722	0.0
22	2.373107002208176E-4	0.0	0.0	9.449593427442847	0.0
23	2.373107002208176E-4	0.0	0.0	9.849699268015145	0.0
24	2.373107002208176E-4	0.0	0.0	10.154643517798895	0.0
25	3.559660503312264E-4	0.0	0.0	10.444043916718183	0.0
26	3.559660503312264E-4	0.0	0.0	10.70449241021053	0.0
27	3.559660503312264E-4	0.0	0.0	10.98333248296999	0.0
28	4.746214004416352E-4	0.0	0.0	11.26751204648442	0.0
29	4.746214004416352E-4	0.0	0.0	11.57554133537104	0.0
30	4.746214004416352E-4	0.0	0.0	11.934117803404696	0.0
31	4.746214004416352E-4	0.0	0.0	12.24902910259772	0.0
32	4.746214004416352E-4	0.0	0.0	12.566432164143064	0.0
33	5.93276750552044E-4	0.0	0.0	12.874105486979355	0.0
34	5.93276750552044E-4	0.0	0.0	13.18913544152249	0.0
35	5.93276750552044E-4	0.0	0.0	13.510216818921256	0.0
36	5.93276750552044E-4	0.0	0.0	13.81883938455843	0.0
37	5.93276750552044E-4	0.0	0.0	14.139327485206644	0.0
38	5.93276750552044E-4	0.0	0.0	14.47144381016568	0.0
39	5.93276750552044E-4	0.0	0.0	14.8000004746214	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTACG	20	7.032214E-4	45.0	1
CGGTCTA	20	7.032214E-4	45.0	31
ACGTAAT	20	7.032214E-4	45.0	11
ATCCGAT	30	2.1648102E-6	44.999996	30
ATGCGTT	30	2.1648102E-6	44.999996	27
GTCGAAT	120	0.0	41.249996	43
CGTCGAA	60	3.6379788E-12	41.249996	42
CGATGAA	700	0.0	41.142857	19
CGTAAGG	55	6.002665E-11	40.909092	2
GACACGA	185	0.0	40.135136	25
TCGTTAG	45	1.9272193E-8	40.0	1
CACGACC	180	0.0	40.0	27
AATACGG	90	0.0	40.0	2
TTATCGC	40	3.456753E-7	39.375	31
TACGCAT	40	3.456753E-7	39.375	16
ATGGTCG	40	3.456753E-7	39.375	44
TGCGTTG	40	3.456753E-7	39.375	1
AACACGT	180	0.0	38.75	41
GCGAATG	35	6.246304E-6	38.57143	1
TGAAGCG	35	6.246304E-6	38.57143	1
>>END_MODULE