FastQCFastQC Report
Sat 18 Jun 2016
SRR3553630_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553630_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806117
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCG26930.3340706125785711No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20660.25629033998786777No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGC16670.20679380288469293No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTC15630.19389244985529397No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15350.19041900865507116No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCC9270.11499571402166188No Hit
GCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC8890.11028175810707379TruSeq Adapter, Index 15 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8080.10023358892071497No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGAA253.889766E-545.09
GTTAGCG253.889766E-545.01
GCCGATT502.1827873E-1145.09
CGCTCAC253.889766E-545.034
TGCGATT207.032032E-445.032
TTACGAG406.8102963E-945.01
TATTGCG302.164692E-644.9999961
TCTACGG650.044.9999962
TTACGCG302.164692E-644.9999961
CTCGAAT1600.042.187543
CGCTAGG650.041.538462
TCCCGAT650.041.5384639
GGCCGAT1200.041.2499968
GTATCCG603.6379788E-1241.24999629
GCGTATG603.6379788E-1241.2499961
CGGACTG603.6379788E-1241.24999640
ACGTAAG501.0804797E-940.51
GCGTACG501.0804797E-940.51
TGCGAAG451.9270374E-840.01
TAGCGAG800.039.3751