Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553630_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 806117 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCG | 2693 | 0.3340706125785711 | No Hit |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 2066 | 0.25629033998786777 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGC | 1667 | 0.20679380288469293 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTC | 1563 | 0.19389244985529397 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1535 | 0.19041900865507116 | No Hit |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCC | 927 | 0.11499571402166188 | No Hit |
GCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC | 889 | 0.11028175810707379 | TruSeq Adapter, Index 15 (95% over 22bp) |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 808 | 0.10023358892071497 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CAACGAA | 25 | 3.889766E-5 | 45.0 | 9 |
GTTAGCG | 25 | 3.889766E-5 | 45.0 | 1 |
GCCGATT | 50 | 2.1827873E-11 | 45.0 | 9 |
CGCTCAC | 25 | 3.889766E-5 | 45.0 | 34 |
TGCGATT | 20 | 7.032032E-4 | 45.0 | 32 |
TTACGAG | 40 | 6.8102963E-9 | 45.0 | 1 |
TATTGCG | 30 | 2.164692E-6 | 44.999996 | 1 |
TCTACGG | 65 | 0.0 | 44.999996 | 2 |
TTACGCG | 30 | 2.164692E-6 | 44.999996 | 1 |
CTCGAAT | 160 | 0.0 | 42.1875 | 43 |
CGCTAGG | 65 | 0.0 | 41.53846 | 2 |
TCCCGAT | 65 | 0.0 | 41.53846 | 39 |
GGCCGAT | 120 | 0.0 | 41.249996 | 8 |
GTATCCG | 60 | 3.6379788E-12 | 41.249996 | 29 |
GCGTATG | 60 | 3.6379788E-12 | 41.249996 | 1 |
CGGACTG | 60 | 3.6379788E-12 | 41.249996 | 40 |
ACGTAAG | 50 | 1.0804797E-9 | 40.5 | 1 |
GCGTACG | 50 | 1.0804797E-9 | 40.5 | 1 |
TGCGAAG | 45 | 1.9270374E-8 | 40.0 | 1 |
TAGCGAG | 80 | 0.0 | 39.375 | 1 |