##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553630_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 806117 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.97970145772884 33.0 31.0 34.0 30.0 34.0 2 32.05717904472924 33.0 31.0 34.0 30.0 34.0 3 32.04772756312049 33.0 31.0 34.0 30.0 34.0 4 35.717429355788305 37.0 35.0 37.0 33.0 37.0 5 35.6267390465652 37.0 35.0 37.0 33.0 37.0 6 35.61728012186816 37.0 35.0 37.0 33.0 37.0 7 35.94524616153735 37.0 35.0 37.0 35.0 37.0 8 35.8651138730482 37.0 35.0 37.0 35.0 37.0 9 37.58424769605405 39.0 37.0 39.0 35.0 39.0 10 37.00940682307903 39.0 37.0 39.0 33.0 39.0 11 36.92873367017443 39.0 37.0 39.0 33.0 39.0 12 36.919047731284664 39.0 37.0 39.0 33.0 39.0 13 36.930368668567965 39.0 37.0 39.0 33.0 39.0 14 38.04491531626302 40.0 37.0 41.0 33.0 41.0 15 38.15893474520448 40.0 37.0 41.0 33.0 41.0 16 38.21262670307164 40.0 37.0 41.0 33.0 41.0 17 38.12047630802973 40.0 37.0 41.0 33.0 41.0 18 38.11069360899224 40.0 37.0 41.0 33.0 41.0 19 38.06908550495772 40.0 37.0 41.0 34.0 41.0 20 37.963077320041634 40.0 37.0 41.0 33.0 41.0 21 37.876980636805825 40.0 36.0 41.0 33.0 41.0 22 37.88182236573599 40.0 36.0 41.0 33.0 41.0 23 37.75077315079573 40.0 36.0 41.0 33.0 41.0 24 37.769042210994186 40.0 36.0 41.0 33.0 41.0 25 37.687142189037075 40.0 36.0 41.0 33.0 41.0 26 37.6155123884002 40.0 36.0 41.0 33.0 41.0 27 37.56212931869691 40.0 36.0 41.0 33.0 41.0 28 37.48749499142184 40.0 36.0 41.0 33.0 41.0 29 37.46009450241094 40.0 36.0 41.0 33.0 41.0 30 37.32793006474246 39.0 36.0 41.0 32.0 41.0 31 37.268695487131524 39.0 35.0 41.0 32.0 41.0 32 37.06979383885962 39.0 35.0 41.0 31.0 41.0 33 36.97446400460479 39.0 35.0 41.0 31.0 41.0 34 36.841981995169434 39.0 35.0 41.0 31.0 41.0 35 36.7498464863041 39.0 35.0 41.0 31.0 41.0 36 36.65669375537298 39.0 35.0 41.0 30.0 41.0 37 36.57073352875575 39.0 35.0 41.0 30.0 41.0 38 36.48370645948417 39.0 35.0 41.0 30.0 41.0 39 36.386417852495356 39.0 35.0 41.0 30.0 41.0 40 36.318375620412425 39.0 35.0 41.0 30.0 41.0 41 36.21460780507048 39.0 35.0 41.0 30.0 41.0 42 36.126419614026254 39.0 35.0 41.0 29.0 41.0 43 36.06923188569401 39.0 35.0 41.0 29.0 41.0 44 35.98408667724412 39.0 35.0 40.0 29.0 41.0 45 35.95928630707453 38.0 35.0 40.0 29.0 41.0 46 35.76891940003746 38.0 35.0 40.0 28.0 41.0 47 35.66465165726563 38.0 35.0 40.0 28.0 41.0 48 35.613241005958194 38.0 35.0 40.0 28.0 41.0 49 35.51933280156602 38.0 34.0 40.0 28.0 41.0 50 35.38976476119471 38.0 34.0 40.0 27.0 41.0 51 33.93998141708958 36.0 32.0 40.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 4.0 10 0.0 11 5.0 12 13.0 13 10.0 14 17.0 15 49.0 16 84.0 17 133.0 18 296.0 19 568.0 20 1062.0 21 1981.0 22 3000.0 23 3724.0 24 4500.0 25 5752.0 26 6992.0 27 8134.0 28 9359.0 29 11137.0 30 13963.0 31 17606.0 32 22765.0 33 30592.0 34 49975.0 35 62526.0 36 64701.0 37 94080.0 38 159407.0 39 233620.0 40 61.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.42219057531351 18.09377546931773 23.056578635607487 13.427455319761275 2 30.821952644591295 26.305114518115857 25.35773343075509 17.51519940653776 3 32.62429647309262 26.052545722271088 26.014958126425817 15.308199678210482 4 27.219870068488817 25.960995736350927 27.488689607091775 19.33044458806848 5 26.283281459143026 30.355271009047076 24.614665116850283 18.746782414959615 6 23.906083112004833 37.81833158214006 23.82222431731374 14.453360988541366 7 83.19995732629383 5.515700574482365 7.913987671764769 3.3703544274590413 8 85.41129885612139 6.139059218450919 5.585293449958257 2.8643484754694417 9 79.65865997119525 7.458222565706964 7.264702270265978 5.61841519283181 10 38.08789542957164 35.10408538710882 13.904929433320474 12.903089749999069 11 29.177030133342928 26.76708219774549 26.18701751730828 17.868870151603303 12 26.217286076338798 24.648903322966763 29.42463687033024 19.709173730364203 13 25.459455637333043 25.514162336236552 30.098236360230583 18.928145666199818 14 21.097805901624703 30.20851811833766 28.700424380083785 19.993251599953854 15 19.93482335690725 27.748453388279863 31.97513512306526 20.341588131747628 16 23.498201873921527 26.472335901612297 30.107540220588326 19.92192200387785 17 23.244640666305262 25.919934699305436 29.0492571177633 21.786167516625998 18 24.04055490704203 26.3235981873599 28.981649065830396 20.654197839767676 19 24.132973253262243 27.579123129769005 27.077086824865372 21.21081679210338 20 26.537090769702164 27.199897781587534 27.22408781851766 19.038923630192638 21 24.984090398788265 27.996060125267174 28.127430633518458 18.8924188424261 22 23.515072874036896 25.80121744114068 28.282743075756994 22.400966609065435 23 22.516954734858587 28.479612760926763 28.18908421482241 20.814348289392235 24 21.530125279581004 27.637799475758484 29.362363031669098 21.469712212991414 25 22.313634373174118 29.221068405702894 28.001146235596075 20.464150985526917 26 21.787035876926055 28.801154174890247 28.40691859866496 21.004891349518743 27 23.307906916737892 27.764828182509486 28.234735156311057 20.69252974444156 28 20.707415921013947 29.300337295950836 29.509612128264262 20.482634654770955 29 22.15323582060669 27.99878925763878 28.605773107377715 21.24220181437682 30 22.25781121102768 28.2854722081286 28.505787621399868 20.95092895944385 31 23.867379052916636 28.4304883782379 26.211951863067025 21.490180705778442 32 23.94193398724999 28.802146586661735 27.539922864795063 19.71599656129321 33 23.241043173633607 28.59944648233445 26.325210856488575 21.834299487543372 34 22.621157970865273 28.508640805242912 27.442170305303076 21.428030918588743 35 22.77771092781817 28.647454401780386 26.666104299996153 21.908730370405287 36 23.53169577120939 30.376607862134158 25.63982647680175 20.4518698898547 37 23.294385306351312 29.407393715800563 26.703567844369985 20.594653133478143 38 22.327280035032135 29.30058539889371 26.380909967163575 21.99122459891058 39 23.016510010333487 28.75910072607326 26.242344473568973 21.982044790024275 40 23.386555549628653 28.350723282104212 27.36128874592646 20.901432422340676 41 21.251753777677436 28.698563608012233 26.853546073336748 23.19613654097358 42 21.883796024646546 28.700548431555223 27.573292710611486 21.842362833186748 43 22.768283015988995 27.45494760686104 27.086638788165985 22.69013058898398 44 22.89382310508276 27.424679047830523 27.49179089387769 22.189706953209026 45 22.6779735447832 27.143578413555353 27.09879583236677 23.07965220929468 46 22.644231544552465 28.844696241364463 26.910981904611862 21.600090309471206 47 21.657774243689193 29.07716869883652 28.06875428752898 21.196302769945305 48 22.104359540860692 28.58381599693345 27.224211869989094 22.08761259221676 49 22.22059576959672 28.0409667579272 28.08066322878689 21.657774243689193 50 21.170996269772253 28.28435574488567 27.750190108879973 22.7944578764621 51 21.52491511778067 27.48806934973459 26.960850596129347 24.026164936355393 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2004.0 1 2201.0 2 2398.0 3 3355.5 4 4313.0 5 3094.0 6 1875.0 7 1895.0 8 1915.0 9 1964.5 10 2014.0 11 2088.0 12 2162.0 13 2266.5 14 2371.0 15 2298.5 16 2226.0 17 2193.0 18 2160.0 19 2144.0 20 2128.0 21 2669.5 22 3211.0 23 3854.0 24 4497.0 25 5403.0 26 7867.0 27 9425.0 28 10789.0 29 12153.0 30 14177.5 31 16202.0 32 19015.5 33 21829.0 34 23670.5 35 25512.0 36 27606.0 37 29700.0 38 33857.0 39 38014.0 40 39020.0 41 40026.0 42 44095.0 43 48164.0 44 50637.0 45 53110.0 46 59231.0 47 65352.0 48 64987.5 49 64623.0 50 62902.0 51 61181.0 52 58498.5 53 55816.0 54 51480.5 55 47145.0 56 43608.0 57 40071.0 58 36603.5 59 33136.0 60 30893.5 61 28651.0 62 26624.5 63 24598.0 64 20543.0 65 16488.0 66 13782.5 67 11077.0 68 8919.5 69 6762.0 70 6023.0 71 5284.0 72 4795.5 73 4307.0 74 3673.0 75 2617.5 76 2196.0 77 1743.5 78 1291.0 79 1059.0 80 827.0 81 584.5 82 342.0 83 229.5 84 117.0 85 80.5 86 44.0 87 29.0 88 14.0 89 14.5 90 15.0 91 12.5 92 10.0 93 7.0 94 4.0 95 6.5 96 9.0 97 4.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 806117.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.264561336566633 #Duplication Level Percentage of deduplicated Percentage of total 1 73.91080036757808 18.67323949321408 2 8.838245739017413 4.465888031621082 3 3.1859535804959065 2.4147515894967877 4 1.7499293209294393 1.768447866531129 5 1.188469559342211 1.5013081039321803 6 0.8883409512791471 1.3466126670825578 7 0.7124516030460724 1.25998440571449 8 0.6095832638470948 1.2320683007367528 9 0.49663735688525557 1.1292592468552087 >10 7.191918386564344 42.83690245382108 >50 1.1451332895099195 18.575336766015592 >100 0.07808850226234183 3.206540928522056 >500 0.0019769241079073884 0.4004430201623923 >1k 0.0024711551348842358 1.1892171262945508 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAATGATACCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCG 2693 0.3340706125785711 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2066 0.25629033998786777 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGC 1667 0.20679380288469293 No Hit GAATCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTC 1563 0.19389244985529397 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1535 0.19041900865507116 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCC 927 0.11499571402166188 No Hit GCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC 889 0.11028175810707379 TruSeq Adapter, Index 15 (95% over 22bp) GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 808 0.10023358892071497 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.06314219896119298 0.0 2 0.0 0.0 0.0 0.2869310534326903 0.0 3 0.0 0.0 0.0 0.3728987231382045 0.0 4 1.2405147143652846E-4 0.0 0.0 0.5296997830339765 0.0 5 1.2405147143652846E-4 0.0 0.0 1.0830933971123298 0.0 6 1.2405147143652846E-4 0.0 0.0 1.369032038773528 0.0 7 1.2405147143652846E-4 0.0 0.0 1.6811455409078335 0.0 8 1.2405147143652846E-4 0.0 0.0 2.103788904092086 0.0 9 1.2405147143652846E-4 0.0 0.0 2.271630544945709 0.0 10 1.2405147143652846E-4 0.0 0.0 3.0623346238821414 0.0 11 1.2405147143652846E-4 0.0 0.0 3.753425371255041 0.0 12 1.2405147143652846E-4 0.0 0.0 4.506789957289079 0.0 13 1.2405147143652846E-4 0.0 0.0 4.72971045146052 0.0 14 1.2405147143652846E-4 0.0 0.0 4.830192143324108 0.0 15 1.2405147143652846E-4 0.0 0.0 5.02594536525095 0.0 16 1.2405147143652846E-4 0.0 0.0 5.3727932793874835 0.0 17 1.2405147143652846E-4 0.0 0.0 5.843692664960545 0.0 18 1.2405147143652846E-4 0.0 0.0 6.343123888964009 0.0 19 1.2405147143652846E-4 0.0 0.0 6.6935692957722015 0.0 20 1.2405147143652846E-4 0.0 0.0 6.9999764302204275 0.0 21 1.2405147143652846E-4 0.0 0.0 7.330449550127339 0.0 22 1.2405147143652846E-4 0.0 0.0 7.743540950010979 0.0 23 1.2405147143652846E-4 0.0 0.0 8.13765247476483 0.0 24 1.2405147143652846E-4 0.0 0.0 8.43922160182703 0.0 25 1.2405147143652846E-4 0.0 0.0 8.706056316887002 0.0 26 1.2405147143652846E-4 0.0 0.0 8.952546590631385 0.0 27 1.2405147143652846E-4 0.0 0.0 9.215039504191079 0.0 28 1.2405147143652846E-4 0.0 0.0 9.492046439908847 0.0 29 1.2405147143652846E-4 0.0 0.0 9.787661096342095 0.0 30 1.2405147143652846E-4 0.0 0.0 10.13947106933609 0.0 31 1.2405147143652846E-4 0.0 0.0 10.437690806669503 0.0 32 1.2405147143652846E-4 0.0 0.0 10.739011830788831 0.0 33 1.2405147143652846E-4 0.0 0.0 11.030036582778926 0.0 34 2.481029428730569E-4 0.0 0.0 11.331605709841128 0.0 35 2.481029428730569E-4 0.0 0.0 11.654263587047538 0.0 36 2.481029428730569E-4 0.0 0.0 11.969230273024884 0.0 37 2.481029428730569E-4 0.0 0.0 12.292632459059913 0.0 38 2.481029428730569E-4 0.0 0.0 12.611196637708918 0.0 39 2.481029428730569E-4 0.0 0.0 12.94204191203014 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAACGAA 25 3.889766E-5 45.0 9 GTTAGCG 25 3.889766E-5 45.0 1 GCCGATT 50 2.1827873E-11 45.0 9 CGCTCAC 25 3.889766E-5 45.0 34 TGCGATT 20 7.032032E-4 45.0 32 TTACGAG 40 6.8102963E-9 45.0 1 TATTGCG 30 2.164692E-6 44.999996 1 TCTACGG 65 0.0 44.999996 2 TTACGCG 30 2.164692E-6 44.999996 1 CTCGAAT 160 0.0 42.1875 43 CGCTAGG 65 0.0 41.53846 2 TCCCGAT 65 0.0 41.53846 39 GGCCGAT 120 0.0 41.249996 8 GTATCCG 60 3.6379788E-12 41.249996 29 GCGTATG 60 3.6379788E-12 41.249996 1 CGGACTG 60 3.6379788E-12 41.249996 40 ACGTAAG 50 1.0804797E-9 40.5 1 GCGTACG 50 1.0804797E-9 40.5 1 TGCGAAG 45 1.9270374E-8 40.0 1 TAGCGAG 80 0.0 39.375 1 >>END_MODULE