FastQCFastQC Report
Sat 18 Jun 2016
SRR3553628_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553628_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611913
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCG145212.3730497636101866No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC82031.340550045513006No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGC62971.0290678576856513No Hit
GCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC27120.44320025886032816No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCT26170.42767517604626804No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22280.3641040474708006No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCGTGTATT22260.36377720362208354No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18810.3073966397183913No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTC17490.2858249457030656No Hit
GAACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT15450.2524868731339259No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTA15340.2506892319659821No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12830.20967032895199156No Hit
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC12370.20215292043149927No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC11650.19038654187768522No Hit
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGCT10770.17600541253413476No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCC8920.14577235652780707No Hit
TCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC6770.11063664279072352No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGCG600.045.0000041
CGAATTC302.1638316E-645.00000444
CCGAACT302.1638316E-645.00000411
TCGCGAC302.1638316E-645.0000041
CTTACCG302.1638316E-645.00000425
CGTATTA207.0306985E-445.017
TTTACGT207.0306985E-445.036
ACGTTTA207.0306985E-445.039
CGCTCGA207.0306985E-445.042
AACGCTC207.0306985E-445.022
CTCGAAT351.210683E-745.043
AACTCGT207.0306985E-445.039
CGCCGTG207.0306985E-445.045
GCGTCTA207.0306985E-445.034
CGTTTAG207.0306985E-445.040
CGTCAAT406.8066583E-945.014
TGAGACG207.0306985E-445.013
AACACGC351.210683E-745.028
AACGTCG253.888661E-544.99999617
ACTTAGA253.888661E-544.99999620